Cell Structure and Microscopy Quiz
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Questions and Answers

What is the primary function of chloroplasts in plant cells?

  • Protein synthesis
  • Photosynthesis (correct)
  • Storage of organic compounds
  • Cell division
  • Which structure is unique to animal cells?

  • Central vacuole
  • Centriole (correct)
  • Cell wall
  • Chloroplasts
  • What role does the large central vacuole play in plant cells?

  • Photosynthesis
  • Storage of nutrients and waste products (correct)
  • DNA replication
  • Respiration
  • How do plasmodesmata function in plant cells?

    <p>They allow communication between neighboring cells. (A)</p> Signup and view all the answers

    What does the Golgi apparatus primarily function in?

    <p>Protein sorting and distribution (C)</p> Signup and view all the answers

    Which of the following cell organelles is considered to be the most abundant in cells?

    <p>Mitochondria (D)</p> Signup and view all the answers

    What is the composition of the plant cell wall primarily made of?

    <p>Cellulose (B)</p> Signup and view all the answers

    What structure in the nucleus is involved in the production of ribosomes?

    <p>Nucleolus (B)</p> Signup and view all the answers

    What is the basic unit of length in the International System of Units (SI units)?

    <p>Meter (B)</p> Signup and view all the answers

    Which of the following is the correct representation of one millionth of a meter?

    <p>10^-6 (C)</p> Signup and view all the answers

    What does magnification represent in microscopy?

    <p>The number of times larger an image is compared to the real object (B)</p> Signup and view all the answers

    What is resolution in the context of microscopy?

    <p>The ability to distinguish between two separate points (D)</p> Signup and view all the answers

    What must be done before determining the size of a specimen using an eyepiece graticule?

    <p>Calibrate the eyepiece graticule (D)</p> Signup and view all the answers

    When using a stage micrometer, what does the equation 'Stage micrometer scale = Eyepiece graticule division' help determine?

    <p>The actual size of the specimen (A)</p> Signup and view all the answers

    Which type of microscope provides a maximum resolution of 200 nm?

    <p>Light microscope (D)</p> Signup and view all the answers

    What happens to wavelengths as energy increases in the electromagnetic spectrum?

    <p>Wavelengths become shorter (D)</p> Signup and view all the answers

    What is a key structural difference between prokaryotic and eukaryotic DNA?

    <p>Eukaryotic DNA is linear and associated with proteins, forming chromosomes. (A)</p> Signup and view all the answers

    Which component is NOT found in prokaryotic cells?

    <p>80s ribosomes (D)</p> Signup and view all the answers

    What role do pili play in prokaryotic cells?

    <p>They assist in the attachment to surfaces. (C)</p> Signup and view all the answers

    What is the main reason viruses are classified as parasitic?

    <p>They must infect living cells to replicate. (D)</p> Signup and view all the answers

    Which of the following correctly describes eukaryotic cells?

    <p>They can measure up to 40 μm in diameter. (D)</p> Signup and view all the answers

    What feature distinguishes viruses from bacteria?

    <p>Viruses can only reproduce inside living cells. (C)</p> Signup and view all the answers

    What do the ribosomes of prokaryotic cells consist of?

    <p>70s subunits (C)</p> Signup and view all the answers

    What is the function of the flagellum in prokaryotic cells?

    <p>It is involved in locomotion. (D)</p> Signup and view all the answers

    What happens to an object smaller than half the wavelength of radiation used to view it?

    <p>It cannot be separated from nearby objects. (A)</p> Signup and view all the answers

    What is the primary function of the nucleus in a cell?

    <p>To control enzyme production and regulate cell functions. (B)</p> Signup and view all the answers

    What distinguishes rough endoplasmic reticulum from smooth endoplasmic reticulum?

    <p>Rough ER has ribosomes, while smooth ER does not. (A)</p> Signup and view all the answers

    What role does the Golgi apparatus play in cellular processes?

    <p>It collects, processes, and sorts molecules for transport. (C)</p> Signup and view all the answers

    What is the primary function of lysosomes in a cell?

    <p>To digest unwanted structures within the cell. (B)</p> Signup and view all the answers

    How do microtubules contribute to cell structure?

    <p>They are long, rigid tubes that make up the cytoskeleton. (D)</p> Signup and view all the answers

    What is the function of centrioles in a cell?

    <p>To grow spindle fibers used for nuclear division. (A)</p> Signup and view all the answers

    What are the main components found in cilia and flagella?

    <p>Microtubules covered by an extension of the plasma membrane. (A)</p> Signup and view all the answers

    According to the endosymbiont theory, what unique characteristic do mitochondria and chloroplasts share?

    <p>They contain smaller ribosomes similar to those found in bacteria. (D)</p> Signup and view all the answers

    What is the diameter of a typical mitochondrion?

    <p>Around 1 μm. (A)</p> Signup and view all the answers

    Flashcards

    Light Microscopy

    A type of microscope that uses light to illuminate and magnify objects.

    Photomicrograph

    A microscope image of a specimen.

    Plant vs. Animal Cell: Size

    Plant cells are typically larger than animal cells and have several unique features.

    Plant Cell: Cell Wall

    Plant cells possess a cell wall, providing structural support and preventing bursting.

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    Plant Cell: Vacuole

    Large, fluid-filled sacs in plant cells that store various substances.

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    Plant Cell: Chloroplast

    Green organelles in plant cells where photosynthesis takes place.

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    Mitochondria

    Organelles responsible for aerobic respiration, providing energy to the cell.

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    Golgi Apparatus

    The Golgi apparatus sorts and distributes molecules within the cell. It acts as a complex packaging and distribution system.

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    Resolution (in microscopy)

    The ability to distinguish between two separate points. If two objects are closer together than the resolution of the apparatus used, then the objects cannot be distinguished as separate.

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    Magnification

    The number of times larger an image is compared with the real size of the object. Magnification = I (size of image) / A (actual size of specimen)

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    Eyepiece Graticule

    A transparent scale placed in the eyepiece of a microscope, used for measuring cells and organelles.

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    Stage Micrometer

    A calibrated scale on the stage of a microscope used to determine the size of each division on the eyepiece graticule.

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    Transmission Electron Microscope (TEM)

    A type of electron microscope that transmits a beam of electrons through a thin slice of a specimen, producing a high-resolution image.

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    Scanning Electron Microscope (SEM)

    A type of electron microscope that scans a beam of electrons across the surface of a specimen, generating a 3D-like image.

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    Electromagnetic Spectrum

    The range of wavelengths of electromagnetic radiation, ranging from very long radio waves to very short gamma rays.

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    Meter

    The fundamental unit of length in the International System of Units (SI units), symbolized by 'm.'

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    Limit of Resolution

    The smallest detail that can be distinguished by a microscope, determined by the wavelength of the light used.

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    Ultrastructure of a Cell

    The detailed structure of a cell visible under an electron microscope, revealing tiny organelles and their internal components.

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    What is the role of the nucleus?

    The control center of the cell, containing genetic material (DNA) and regulating cell activities.

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    What does the endoplasmic reticulum do?

    A network of interconnected membranes throughout the cytoplasm, involved in protein synthesis, lipid production, and transport.

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    What is the function of the Golgi apparatus?

    A stack of flattened sacs involved in processing and sorting molecules for transport within the cell or secretion outside.

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    What is the function of lysosomes?

    Spherical sacs containing enzymes that break down waste materials, old organelles, and engulfed bacteria.

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    What is the role of mitochondria?

    An organelle with a double membrane, responsible for generating energy for the cell through cellular respiration.

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    What is the function of microtubules?

    Long, hollow tubes made of tubulin protein, forming the structural framework of the cell and supporting its shape.

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    What do centrioles do?

    Small cylindrical structures near the nucleus, responsible for organizing microtubules during cell division.

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    What are microvilli, cilia, and flagella?

    Finger-like extensions of the cell membrane, increasing surface area for absorption, while cilia and flagella are long, thin extensions for movement.

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    What are prokaryotic cells?

    Prokaryotic cells are simple cells lacking a nucleus and other membrane-bound organelles. They include bacteria and are generally smaller than eukaryotic cells.

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    What are eukaryotic cells?

    Eukaryotic cells are complex cells with a nucleus and other membrane-bound organelles. They include animals, plants, fungi, and protists.

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    What are mitochondria?

    Mitochondria are organelles found in both plant and animal cells. They are responsible for cellular respiration, a process that generates energy for the cell.

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    What are chloroplasts?

    Chloroplasts are organelles found in plant cells. They are responsible for photosynthesis, a process that uses sunlight to convert carbon dioxide and water into glucose (sugar) and oxygen.

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    What are viruses?

    Viruses are non-living entities that are much smaller than bacteria. They lack a cellular structure and can only reproduce by infecting other living cells.

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    What is a capsid?

    A capsid is a protective protein coat that surrounds the genetic material of a virus. It helps the virus to survive and infect host cells.

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    What are plasmids?

    Plasmids are small, circular pieces of DNA found in some bacteria. They can replicate independently of the main bacterial chromosome and often carry genes for antibiotic resistance.

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    What are pili?

    Pili are hair-like structures found on the surface of some bacteria. They help bacteria to attach to surfaces and other cells, and can also be involved in sexual reproduction.

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    Study Notes

    Cell Structure - Microscopy

    • Light microscopy is used to view cells.
    • Components of a light microscope include: body tube, revolving nosepiece, objectives, stage clips, diaphragm, light source, stage, coarse adjustment knob, fine adjustment knob, and base, ocular lens (eyepiece).
    • Light microscopes utilize light to illuminate the specimen and form an image.

    Cell Structure - Photomicrographs

    • Photomicrographs are images of cells taken using a microscope.
    • Examples shown include plant cells, cheek cells, and guard cells.

    Cell Structure - Plant vs. Animal Cells

    • Plant and animal cells share similarities but also have differences in structures.
    • Structures found in plant cells but not animal cells include a cell wall, chloroplasts, and a large central vacuole.
    • Structures found in animal cells but not plant cells include centrioles.
    • Both plant and animal cells have cell membranes, cytoplasm, mitochondria, and Golgi apparatus.
    • Both types of cells use staining techniques to highlight cell structures.

    Cell Structure - Measuring Cells

    • Measuring objects in microscopy uses units like millimeters (mm), micrometers (µm), and nanometers (nm).
    • The international system uses the meter (m), as its basic unit of length.

    Cell Structure - Magnification and Resolution

    • Magnification is the increase in size of an image as compared to the size of the specimen.
    • Resolution is the ability to distinguish two points that are extremely close as separate.
    • Units for magnification and resolution need to be consistent ( e.g., nm for both ).
    • A light microscope has a maximum resolution of 200nm.

    Cell Structure - Measuring Cells with Graticules

    • Eye-piece graticules are transparent scales placed in the eyepiece of a microscope to measure cell and organelle sizes.
    • A stage micrometer scale is used to calibrate the eyepiece graticule. This is done by placing the stage micrometer on the stage of the microscope, focusing, and aligning the scales.
    • The value of the eyepiece graticule division must be calculated using the scale values of the stage micrometer.
    • To measure a sample, determine how many divisions are present in the specimen and use the calculated value to find the actual specimen size.

    Cell Structure - Electron Microscopy

    • Transmission electron microscopes (TEM) and scanning electron microscopes (SEM) provide higher resolution than light microscopes to view cell ultrastructures.

    Cell Structure - The Electromagnetic Spectrum

    • The electromagnetic spectrum is the range of wavelengths of electromagnetic radiation.
    • The shorter the wavelength, the higher the energy of the radiation.
    • Microscopes use specific forms of electromagnetic radiation.

    Cell Structure - Cell Ultrastructure

    • Ultrastructure refers to the detailed structures revealed by the electron microscope.
    • Cell components that are viewed by the electron microscope include plant cells with peroxisomes, mitochondria, golgi complex, microfilm filaments, vacuoles, microtubules, chloroplasts, cytoplasm, ribosomes, smooth endoplasmic reticulum, rough endoplasmic reticulum, plasma membrane, cell wall, and nucleus.
    • Animal cells contain similar structures, but there are differences (e.g., centrioles, lysosomes ).

    Cell Structure - Nucleus

    • Nucleus is a double-membraned, rounded organelle embedded in the cytoplasm.
    • The nucleus controls cell division and regulation of chemical changes inside the cell, to determine its role.
    • The nucleus holds chromatin, and chromosomes, which play a role in cell division and inheritance

    Cell Structure - Endoplasmic Reticulum

    • The Endoplasmic Reticulum (ER) is an extensive membrane system in the cytoplasm that forms flattened sacs, called cisternae.
    • The Rough ER contains ribosomes for protein production and transport.
    • The Smooth ER makes lipids and steroids.

    Cell Structure - Golgi Body

    • The Golgi body is a stack of flattened sacs formed from vesicles budding off the ER.
    • The Golgi body sorts molecules being transported to other parts of the cell.
    • Lysosomes are formed from the Golgi body.

    Cell Structure - Lysosomes

    • Lysosomes are spherical sacs with a single membrane.
    • They contain hydrolytic enzymes that digest unwanted structures within the cell or old organelles. They can digest entire cells or bacteria.

    Cell Structure - Mitochondrion

    • Mitochondria are surrounded by a double membrane.
    • They are about 1 micrometer in size, and contain cristae, folded inner membranes.
    • Mitochondria release energy from food substances through cellular respiration. They convert that energy into ATP.
    • They are involved in lipid synthesis.

    Cell Structure - Microtubules

    • Microtubules are long tubular structures in the cytoplasm, forming the cell's cytoskeleton.
    • They are made up of tubulin protein that forms a cylinder with a hollow center.
    • They are part of the microtubule organizing centers (MTOCs).

    Cell Structure - Centrioles

    • Centrioles are hollow cylinders formed from microtubules to grow spindle fibres for nuclear division.
    • They are found near the animal cell nucleus.
    • Each centriole consists of nine triplets of microtubules.

    Cell Structure - Cilia, Flagella & Microvilli

    • Microvilli are finger-like extensions of the cell membrane that increase the surface area for absorption.
    • Cilia and flagella are long, thin extensions covered by plasma membrane and contain microtubules for movement.
    • Flagella are typically fewer in number than cilia.

    Cell Structure - The Endosymbiont Theory

    • Mitochondria and chloroplasts are thought to have originated from ancient bacteria that were engulfed by other cells.
    • They contain their own DNA and ribosomes, similar to bacteria.

    Cell Structure - Two Fundamentally Different Cell Types

    • Prokaryotic cells do not contain a nucleus. Examples include bacteria.
    • Eukaryotic cells contain a nucleus. Examples include plants, animals, fungi, and protists.
    • Prokaryotes cell walls contain murein, while eukaryotic cells may have different types of cell walls.

    Cell Structure - Prokaryotic Cell

    • Pili help with attachment to other cells for reproduction.
    • Cell walls contain the murein (peptidoglycan) protein.
    • Flagella are used for locomotion.
    • Plasmids are small, circular DNA.
    • Capsules are for additional protection.
    • Ribosomes are 70s.

    Cell Structure - Viruses

    • Viruses are smaller than bacteria and at the boundary between living and non-living organisms.
    • Viruses consist of a self-replicating molecule of DNA or RNA (genetic code) and a protein coat (capsid).
    • Viruses range in size from 20-300 nm.
    • Viruses are parasitic, needing a host cell to reproduce. They infect other living cells.

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    Chapter 1 - Cell Structure PDF

    Description

    This quiz covers essential concepts of cell structure, focusing on light microscopy and the differences between plant and animal cells. It explores the components of light microscopes, the importance of photomicrographs, and unique cellular structures of each type of cell. Test your understanding of these fundamental biological concepts.

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