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Questions and Answers
What does magnification do to the image of a specimen?
What does magnification do to the image of a specimen?
Magnification enlarges the image of a specimen.
What is resolving power?
What is resolving power?
Resolving power is the ability of a microscope to distinguish two points as separate entities.
What is a disadvantage of the electron microscope?
What is a disadvantage of the electron microscope?
Samples must be placed in a vacuum and are typically fixed, dehydrated, and sometimes coated with heavy metals, which means you cannot view living cells.
What type of electron microscopy provides 3D images of a specimen's surface topography?
What type of electron microscopy provides 3D images of a specimen's surface topography?
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What type of electron microscopy produces detailed, 2D images of thin section, revealing internal structures at high resolution?
What type of electron microscopy produces detailed, 2D images of thin section, revealing internal structures at high resolution?
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What is one of the smallest organelles isolated in cell fractionation?
What is one of the smallest organelles isolated in cell fractionation?
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What are the two domains with prokaryotic cells?
What are the two domains with prokaryotic cells?
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In prokaryotes, DNA is enclosed within a nucleus.
In prokaryotes, DNA is enclosed within a nucleus.
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What rigid structure provides shape and protection to the cell?
What rigid structure provides shape and protection to the cell?
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What regulates material exchange in the cell?
What regulates material exchange in the cell?
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What part is the region where the chromosome is located?
What part is the region where the chromosome is located?
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What is the site of protein synthesis?
What is the site of protein synthesis?
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What structure is used for motility?
What structure is used for motility?
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As a cell increases in size, what grows faster, its volume or its surface area?
As a cell increases in size, what grows faster, its volume or its surface area?
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What is the function of microvilli?
What is the function of microvilli?
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Where does protein folding and modification occur in the endoplasmic reticulum(ER)?
Where does protein folding and modification occur in the endoplasmic reticulum(ER)?
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What do transport vesicles do?
What do transport vesicles do?
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Rough ER is studded with ribosomes, while smooth ER lacks ribosomes.
Rough ER is studded with ribosomes, while smooth ER lacks ribosomes.
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What processes is smooth ER involved in?
What processes is smooth ER involved in?
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What process does chronic alcohol use induce that leads to tolerance?
What process does chronic alcohol use induce that leads to tolerance?
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As secretory proteins are synthesized by ribosomes on the rough ER where do they enter?
As secretory proteins are synthesized by ribosomes on the rough ER where do they enter?
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What does the cis face of the golgi apparatus do?
What does the cis face of the golgi apparatus do?
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Within the golgi apparatus, what region modifies proteins?
Within the golgi apparatus, what region modifies proteins?
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Lysosomes contain what?
Lysosomes contain what?
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What is the process by which cells such as macrophages engulf large particles or pathogens?
What is the process by which cells such as macrophages engulf large particles or pathogens?
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Describe autophagy.
Describe autophagy.
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What is the cause of Tay-Sachs disease?
What is the cause of Tay-Sachs disease?
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What type of vacuole is used for temporary storage for ingested materials in phagocytic cells?
What type of vacuole is used for temporary storage for ingested materials in phagocytic cells?
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What type of vacuole expels excess water in freshwater protists?
What type of vacuole expels excess water in freshwater protists?
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What type of vacuole maintains turgor pressure and store nutrients/waste?
What type of vacuole maintains turgor pressure and store nutrients/waste?
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What does the endosymbiont theroy suggest about mitochodnria and chloroplastss?
What does the endosymbiont theroy suggest about mitochodnria and chloroplastss?
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Mitochondria and chloroplasts have singular membranes.
Mitochondria and chloroplasts have singular membranes.
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Mitochondria and chloroplasts their own circular DNA similar to bacterial genomes.
Mitochondria and chloroplasts their own circular DNA similar to bacterial genomes.
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What is the folds of the inner membrane of a mitochodnria called?
What is the folds of the inner membrane of a mitochodnria called?
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Where does the Calvin cycle take place in the chloroplast?
Where does the Calvin cycle take place in the chloroplast?
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What is the function of mitochondria?
What is the function of mitochondria?
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What is the function of chloroplasts?
What is the function of chloroplasts?
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Peroxisomes help with the breakdown of which molecules?
Peroxisomes help with the breakdown of which molecules?
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What enzyme do peroxisomes user to detoxify hydrogen peroxide?
What enzyme do peroxisomes user to detoxify hydrogen peroxide?
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What are the three types of cytoskeletal fibers?
What are the three types of cytoskeletal fibers?
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What structures are seal adjacent cells to prevent leakage of extracellular?
What structures are seal adjacent cells to prevent leakage of extracellular?
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What structures provide strong adhesion between cells, anchoring intermediate filaments?
What structures provide strong adhesion between cells, anchoring intermediate filaments?
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What structures Allow direct communication by permitting ions and small molecules to pass between cells?
What structures Allow direct communication by permitting ions and small molecules to pass between cells?
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What is the primary function of the cytoskeleton?
What is the primary function of the cytoskeleton?
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In terms of cellular function, what is the most important difference between prokaryotic and eukaryotic cells?
In terms of cellular function, what is the most important difference between prokaryotic and eukaryotic cells?
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The extracellular matrix of the animal cell has all of the following molecular components except which of these?
The extracellular matrix of the animal cell has all of the following molecular components except which of these?
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Flashcards
Magnification
Magnification
The process of enlarging the image of a specimen under a microscope.
Resolving Power
Resolving Power
Ability to distinguish two close points as separate entities in microscopy.
Electron Microscope Disadvantage
Electron Microscope Disadvantage
Cannot view living cells due to vacuum and sample preparation.
Scanning Electron Microscopy (SEM)
Scanning Electron Microscopy (SEM)
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Transmission Electron Microscopy (TEM)
Transmission Electron Microscopy (TEM)
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Cell Fractionation
Cell Fractionation
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Prokaryotic Domains
Prokaryotic Domains
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DNA Location in Prokaryotes
DNA Location in Prokaryotes
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Cell Wall Function
Cell Wall Function
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Plasma Membrane Function
Plasma Membrane Function
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Microvilli Function
Microvilli Function
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Surface Area-to-Volume Ratio
Surface Area-to-Volume Ratio
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Nuclear Envelope
Nuclear Envelope
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Nuclear Lamina
Nuclear Lamina
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Role of Ribosomes
Role of Ribosomes
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Bound vs. Free Ribosomes
Bound vs. Free Ribosomes
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Endomembrane System
Endomembrane System
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Rough ER Function
Rough ER Function
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Smooth ER Functions
Smooth ER Functions
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Golgi Apparatus
Golgi Apparatus
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Lysosomes
Lysosomes
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Phagocytosis
Phagocytosis
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Vacuoles
Vacuoles
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Mitochondria Function
Mitochondria Function
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Chloroplast Function
Chloroplast Function
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Cytoskeleton Definition
Cytoskeleton Definition
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Cilia vs. Flagella
Cilia vs. Flagella
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Intercellular Junctions
Intercellular Junctions
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Brain Cell Function
Brain Cell Function
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Extracellular Matrix (ECM)
Extracellular Matrix (ECM)
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Cell Wall Composition
Cell Wall Composition
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Study Notes
Cell Structure and Function
-
Magnification vs. Resolving Power:
- Magnification enlarges the image of a specimen.
- Resolving power is the ability of a microscope to distinguish two points as separate entities. Higher resolving power yields clearer images.
-
Electron Microscopy Disadvantages:
- Samples must be placed in a vacuum and are typically fixed, dehydrated, and coated with heavy metals. This prevents the study of living cells.
-
Electron Micrograph Types:
- Scanning Electron Microscopy (SEM) produces 3D images of the specimen's surface topography.
- Transmission Electron Microscopy (TEM) produces detailed 2D images of thin sections, revealing internal structures at high resolution.
-
Cell Fractionation:
- Ribosomes are among the smallest organelles isolated through high-speed centrifugation.
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Prokaryotic Domains:
- Bacteria and Archaea are domains with prokaryotic cells.
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DNA Location Difference:
- In prokaryotes, DNA is located in a region called the nucleoid, not enclosed by a membrane.
- In eukaryotes, DNA is enclosed within a nucleus.
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Prokaryotic Cell Diagram Labels:
- Cell Wall: Rigid structure providing shape and protection
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Cell Size and Microvilli:
- As a cell increases in size, its volume grows faster than its surface area, limiting material exchange.
- Microvilli increase the surface area of cells like intestinal epithelial cells to enhance absorption.
Surface Area to Volume Ratio
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Cell A (125 × 1 × 1):
- Volume: 125 cubic units
- Surface Area: approximately 502 square units
- SA:Volume Ratio: approximately 4.02
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Cell B (5 × 5 × 5):
- Volume: 125 cubic units
- Surface Area: approximately 150 square units
- SA:Volume Ratio: approximately 1.2
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Conclusion: Cell A (elongated) has a higher SA:Volume ratio, facilitating efficient material exchange.
Eukaryotic Cell Structures and Functions
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Nuclear Envelope:
- Double membrane enclosing the nucleus.
- Nuclear pores allow molecules to pass between the nucleus and cytoplasm
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Nuclear Lamina and Matrix:
- Nuclear Lamina provides structural support to the inner nuclear membrane.
- Nuclear Matrix organizes chromatin and maintains the shape of the nucleus.
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Chromatin and Condensation:
- Chromatin consists of DNA and histone proteins.
- During interphase, chromatin is loosely organized; during cell division, it condenses into visible chromosomes.
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Nucleolus: Synthesizes ribosomal RNA and assembles ribosomes.
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Ribosomes:
- Function in protein synthesis.
- Two subunits (small and large)
- Free ribosomes synthesize proteins for the cytosol.
- Bound ribosomes synthesize proteins for secretion, membranes, or lysosomes
Endomembrane System
-
Endoplasmic Reticulum (ER):
- Lumen: Interior space for protein folding and modification.
- Transport Vesicles: Shuttle proteins and lipids between ER, Golgi, and other destinations.
- Rough ER: Studded with ribosomes for protein synthesis; smooth ER with no ribosomes for lipid synthesis and detoxification.
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Golgi Apparatus:
- Cis face receives vesicles from the rough ER.
- Modifies proteins (e.g., glycosylation, phosphorylation).
- Trans face sorts and packages proteins into vesicles for secretion.
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Lysosomes:
- Membrane-bound vesicles containing hydrolytic enzymes for breaking down macromolecules.
- Involved in phagocytosis and autophagy.
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Vacuoles:
- Food vacuoles: Temporarily store ingested materials.
- Contractile vacuoles: Expel water in freshwater protists.
- Central vacuoles (in plants): Large, persistent storage organelles.
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Endomembrane System Coordination:
- Proteins synthesized on rough ER are packaged into vesicles transported to the Golgi.
- Golgi modifies and sorts them, dispatching vesicles that fuse with the plasma membrane or lysosomes.
Mitochondria and Chloroplasts
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Mitochondria:
- Double membranes (inner folded into cristae).
- Matrix contains enzymes for cellular respiration.
- Produce ATP (energy).
-
Chloroplast:
- Double membranes.
- Thylakoids where light reactions occur.
- Stroma for the Calvin cycle (photosynthesis).
- Convert light energy into chemical energy.
Cytoskeleton
-
Cytoskeleton:
- Dynamic network of protein fibers maintaining cell shape, positioning organelles, and enabling movement.
- Three types: Microfilaments, Intermediate filaments, Microtubules
- Functions: Maintaining shape, Facilitating movement, Organizing organelles.
-
Microtubules:
- Serve as tracks for motor proteins during vesicle and organelle transport.
- Form the spindle apparatus during cell division.
- Form cilia and flagella.
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Cilia and Flagella:
- Motility (movement).
- Cilia are numerous, short, coordinated movements. Flagella are longer, fewer, whip-like motions.
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Centrioles:
- Organize microtubules and the formation of the mitotic spindle during cell division.
Cell Walls (Plant Cells)
- Cell Wall:
- Provides structural support and protection in plant cells.
- Composed of cellulose (plants). Peptidoglycans (bacteria).
- Primary cell wall is relatively thin and flexible during growth.
- Middle lamella: Cements adjacent plant cells.
- Secondary cell wall in some cells, thicker and rigid, for support.
Extracellular Matrix (Animal cells)
- Extracellular Matrix:
- Comprised of proteins (e.g., collagen, elastin) and glycoproteins.
- Provides structural support, facilitates cell adhesion, and enables intercellular communication
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Description
Explore cell structure, magnification, and resolving power. Learn about electron microscopy, including SEM and TEM techniques. Understand cell fractionation and the differences between prokaryotic domains Bacteria and Archaea and DNA location.