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Questions and Answers
During cellular respiration, what is the theoretical maximum ATP yield from a single glucose molecule?
During cellular respiration, what is the theoretical maximum ATP yield from a single glucose molecule?
The mitochondrial matrix contains several components essential for ATP production. Which of the following is NOT typically found within the mitochondrial matrix?
The mitochondrial matrix contains several components essential for ATP production. Which of the following is NOT typically found within the mitochondrial matrix?
Which of the following is a primary function of the rough endoplasmic reticulum (RER)?
Which of the following is a primary function of the rough endoplasmic reticulum (RER)?
What is the primary function of the Smooth Endoplasmic Reticulum (SER)?
What is the primary function of the Smooth Endoplasmic Reticulum (SER)?
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The endoplasmic reticulum (ER) provides a separate chemical environment that is crucial for which of the following processes?
The endoplasmic reticulum (ER) provides a separate chemical environment that is crucial for which of the following processes?
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Which of the following is NOT a structural component of the double-membraned organelle?
Which of the following is NOT a structural component of the double-membraned organelle?
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The outer membrane of the double-membraned organelle is characterized by:
The outer membrane of the double-membraned organelle is characterized by:
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What is the primary function of the numerous integral proteins, such as porins, present in the outer membrane of the double-membraned organelle?
What is the primary function of the numerous integral proteins, such as porins, present in the outer membrane of the double-membraned organelle?
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Which characteristic of the inner membrane is essential for maintaining a proton gradient?
Which characteristic of the inner membrane is essential for maintaining a proton gradient?
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What is the primary role of the specific transport proteins found in the inner membrane?
What is the primary role of the specific transport proteins found in the inner membrane?
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How do cristae contribute to the function of certain double-membraned organelles?
How do cristae contribute to the function of certain double-membraned organelles?
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The abundance of cristae in double-membraned organelles within certain cells indicates:
The abundance of cristae in double-membraned organelles within certain cells indicates:
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Which of the following best describes the composition of the intermembrane space?
Which of the following best describes the composition of the intermembrane space?
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Which statement accurately describes the distribution of mitochondria across different organisms and cell types?
Which statement accurately describes the distribution of mitochondria across different organisms and cell types?
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A researcher is observing a cell under a microscope and notices a high concentration of mitochondria clustered around a specific area. What is the most likely explanation for this observation?
A researcher is observing a cell under a microscope and notices a high concentration of mitochondria clustered around a specific area. What is the most likely explanation for this observation?
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Which of the following is NOT a characteristic of mitochondria?
Which of the following is NOT a characteristic of mitochondria?
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Which of the following is the primary function of mitochondria within eukaryotic cells?
Which of the following is the primary function of mitochondria within eukaryotic cells?
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If a cell type is observed to have a significantly lower number of mitochondria compared to other cells in the same organism, what can be inferred about its function?
If a cell type is observed to have a significantly lower number of mitochondria compared to other cells in the same organism, what can be inferred about its function?
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A scientist discovers a new single-celled eukaryotic organism in a deep-sea vent. Upon examination, the organism's cells contain mitochondria. What can the scientist infer from this discovery?
A scientist discovers a new single-celled eukaryotic organism in a deep-sea vent. Upon examination, the organism's cells contain mitochondria. What can the scientist infer from this discovery?
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In the context of cellular respiration, which metabolic pathway does NOT directly occur within the mitochondria?
In the context of cellular respiration, which metabolic pathway does NOT directly occur within the mitochondria?
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A cell is treated with a drug that inhibits the function of the electron transport chain. What is the most likely consequence of this treatment?
A cell is treated with a drug that inhibits the function of the electron transport chain. What is the most likely consequence of this treatment?
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What is the significance of the oxidizing environment within the RER lumen?
What is the significance of the oxidizing environment within the RER lumen?
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How do proteins enter the ER lumen during synthesis?
How do proteins enter the ER lumen during synthesis?
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What determines the characteristic 'roughness' of the Rough Endoplasmic Reticulum (RER)?
What determines the characteristic 'roughness' of the Rough Endoplasmic Reticulum (RER)?
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Which of the following best describes the polarity of the Golgi apparatus?
Which of the following best describes the polarity of the Golgi apparatus?
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How does the Golgi apparatus modify structures synthesized in the ER?
How does the Golgi apparatus modify structures synthesized in the ER?
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What is the primary function of lysosomes within the cell?
What is the primary function of lysosomes within the cell?
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Why is the pH within lysosomes maintained at an acidic level (pH 4-5)?
Why is the pH within lysosomes maintained at an acidic level (pH 4-5)?
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Flashcards
Mitochondria
Mitochondria
Organelles that generate energy through aerobic respiration.
Aerobic Respiration
Aerobic Respiration
Process used by mitochondria that converts glucose and oxygen into energy.
Krebs Cycle
Krebs Cycle
A series of chemical reactions in mitochondria that produce energy carriers.
Electron Transport Chain
Electron Transport Chain
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Cell Types with Mitochondria
Cell Types with Mitochondria
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Mitochondrial Variation
Mitochondrial Variation
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Size of Mitochondria
Size of Mitochondria
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Shape Changes
Shape Changes
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Mitochondrial Matrix
Mitochondrial Matrix
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Endoplasmic Reticulum (ER)
Endoplasmic Reticulum (ER)
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Rough ER (RER)
Rough ER (RER)
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Smooth ER (SER)
Smooth ER (SER)
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Chloroplast
Chloroplast
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Nucleus
Nucleus
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Outer membrane
Outer membrane
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Intermembrane space
Intermembrane space
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Inner membrane
Inner membrane
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Cristae
Cristae
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Oxidative phosphorylation
Oxidative phosphorylation
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Transport proteins
Transport proteins
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Rough Endoplasmic Reticulum (RER)
Rough Endoplasmic Reticulum (RER)
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Functions of RER
Functions of RER
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Golgi Apparatus
Golgi Apparatus
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Polarity of Golgi
Polarity of Golgi
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Protein Modification in Golgi
Protein Modification in Golgi
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Lysosomes
Lysosomes
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Importance of Golgi
Importance of Golgi
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Cytoskeleton
Cytoskeleton
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Study Notes
Cell Organelles
- Cell organelles perform specific functions within the cell
- Mitochondria are the powerhouses of the cell, responsible for energy production through aerobic respiration (glycolysis, Krebs cycle, electron transport chain)
Mitochondria (MT)
- Found in animal and plant cells (excluding red blood cells), not bacteria
- Number varies based on the cell's metabolic demands (algae and fungi have one to a few, liver cells have 500-1000 per cell)
- Located throughout the cytoplasm, concentrated where energy is needed most, among muscle myofibrils
Mitochondrial Structural Features
- Vary in shape (spherical, oval, rod-like, elongated)
- Typically 0.5-1 µm in diameter and 1-2 µm in length (second largest organelle in the cell)
- Similar in size to a bacterium
- Double-membraned organelle
- Outer membrane: single continuous layer, relatively low protein content than plasma membrane, has large protein channels (porins) for transporting small molecules and ions
- Intermembrane space: space between the outer and inner membranes. Contains common ions and sugars similar to cytoplasm, but with different protein composition (e.g., cytochrome c)
- Inner membrane: highly folded (cristae) to increase surface area, highly permeable to small molecules like O2, CO2, and H2O but impermeable to most ions. Has high protein content (~75%), contains specific transport proteins to regulate movement of materials in and out of the matrix and perform oxidative phosphorylation, generating ATP (energy molecule)
- Matrix: semifluidic, contains ~30% of all mitochondrial proteins, including enzymes for ATP production (e.g., Krebs cycle), oxidation of pyruvate and fatty acids, ribosomes, tRNA, and mitochondrial DNA genome
Cellular Respiration
- One glucose molecule can generate 38 ATP molecules
- Glycolysis (cytoplasm), Krebs Cycle (matrix), Electron Transport Chain (inner membrane)
Endoplasmic Reticulum (ER)
- Internal network of membranes within the cytoplasm
- Originates from the outer nuclear membrane
- Eukaryotic cells, important for protein processing.
- Rough ER (RER): studded with ribosomes; site for protein synthesis, folding and modification, and transport (protein secretory pathway) proteins destined for secretion
- Smooth ER (SER): lacks ribosomes; synthesizes lipids, stores calcium, involved in detoxification
Golgi Apparatus
- Highly curved, tubular structure
- Modifies, sorts, and packages proteins and lipids received from the ER for secretion or use within the cell.
- Involved in secretion, a prominent structure in cells with secretory functions (e.g., epithelial cells)
- Plays a key role in modifying molecules previously synthesized in the ER (e.g., modification of carbohydrate groups using enzymes at each cisternae)
Lysosomes
- Membrane-bound organelles containing hydrolytic enzymes
- Involved in digestion, recycling of cellular material, and breakdown of captured particles (e.g. phagocytosis, endocytosis)
- Enzymes are activated in the Golgi body, and enclosed in a lipid membrane with glycosylated transport proteins
Importance of Golgi apparatus
- Golgi body (apparatus) interacts mostly with the endoplasmic reticulum (ER), plasma membrane (PM), and lysosomes of the cell
- Glycosylation in the Golgi (in addition to in ER) is the mechanism by which proteins are targeted to their correct locations within the cell
- Cell death occurs without Golgi bodies, as there are no lysosomes for breaking down substances
- Proteins can't travel to their correct locations without Golgi apparatus
Cytoskeleton
- Network of proteins (microtubules, actin filaments, intermediate filaments) in the cytoplasm
- Provides structural support and framework
- Allows movement of molecules and organelles
- Functions include:
- Cellular shape, supporting structure, motility
- Mechanical strength and integrity
Differences in Cytoskeletal components
-
Microfilaments (actin): involved in cell shape, muscle contraction, cell division
-
Intermediate filaments (keratin, vimentin): maintain cell shape and anchor nucleus
-
Microtubules: involved in cell shape, intracellular transport, cell motility, and cell division (e.g. chromosome movement, cilia, flagella movement)
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Description
Discusses the function of cell organelles, focusing on mitochondria as the cell's powerhouses. Explains their role in energy production through aerobic respiration and describes their structural features, including the double-membrane.