Cell Membrane Structure and Functions Quiz

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Questions and Answers

What does the asymmetry of protein distribution in membranes indicate?

  • All proteins are equally distributed.
  • Proteins are located based on their function. (correct)
  • Only lipids show asymmetry.
  • Proteins can easily switch layers.

Carbohydrate motifs in the plasma membrane are directed inwardly.

False (B)

What enzyme is responsible for the displacement of lipids from one membrane layer to another?

flipases

The majority of phosphatidylcholine is located in the _______ leaflet of red blood cells.

<p>outer</p> Signup and view all the answers

Which of the following protein types is likely to be found in the outer leaflet?

<p>Proteins mediating attachment to the extracellular matrix (D)</p> Signup and view all the answers

The inner leaflet of a cell membrane tends to have a more positive charge than the outer leaflet due to lipid distribution.

<p>False (B)</p> Signup and view all the answers

Name one factor that affects membrane fluidity.

<p>Temperature</p> Signup and view all the answers

Match the following proteins or lipids with their corresponding leaflet distribution:

<p>Phosphatidylcholine = Outer leaflet Phosphatidylethanolamine = Inner leaflet Phosphatidylserine = Inner leaflet Disulfide bond proteins = Outer leaflet</p> Signup and view all the answers

What did Frye and Edidin use to facilitate the formation of heterokaryon in their experiment?

<p>Sendai Virus (C)</p> Signup and view all the answers

Proteins in the membrane are fixed in precise positions and do not move laterally.

<p>False (B)</p> Signup and view all the answers

What is the maximum lateral movement speed of a phospholipid molecule in the membrane?

<p>2 μm per second</p> Signup and view all the answers

The movement of proteins between the two layers of the membrane is almost impossible without the intervention of __________.

<p>flipases</p> Signup and view all the answers

Match the following terms with their descriptions:

<p>Heterokaryon = Hybrid cell formed from the fusion of two distinct species' cells Fluorescence Microscopy = Technique used to visualize labeled proteins Lateral Movement = Movement of proteins within the same membrane layer Cytoskeleton = Structure that may control the movement of certain membrane proteins</p> Signup and view all the answers

What was observed about the distribution of membrane proteins immediately after the fusion of cells?

<p>They were located at opposite poles. (C)</p> Signup and view all the answers

What is the primary structure of the cell membrane as described in the fluid-mosaic model?

<p>A bimolecular lipid layer (A)</p> Signup and view all the answers

Movement of membrane proteins is always random.

<p>True (A)</p> Signup and view all the answers

Integral proteins exclusively penetrate only the outer layer of the lipid bilayer.

<p>False (B)</p> Signup and view all the answers

What role do extracellular matrix and membrane proteins play in protein movement?

<p>They control the movement of membrane proteins in the same area.</p> Signup and view all the answers

What are peripheral proteins primarily attached to?

<p>The inner and outer polar surfaces of the lipid bilayer</p> Signup and view all the answers

What primarily stabilizes the lipid bilayer structure of cell membranes?

<p>Hydrophobic interactions (C)</p> Signup and view all the answers

Integral proteins must have a stretch rich in ___________ to be stable inside the hydrophobic region of the lipid bilayer.

<p>hydrophobic amino acids</p> Signup and view all the answers

The Gorter and Grindel model explains the organization of proteins in the lipid bilayer.

<p>False (B)</p> Signup and view all the answers

Which type of membrane protein is covalently attached to lipid molecules?

<p>Peripheral proteins (D)</p> Signup and view all the answers

Match the types of membrane proteins with their characteristics:

<p>Peripheral proteins = Weakly attached at polar surfaces Integral proteins = Can penetrate the membrane Extrinsic proteins = Covalently bound to lipids Trimeric G proteins = Anchored by fatty acids</p> Signup and view all the answers

What appearance did Robertson observe in cell membranes using osmium tetroxide staining?

<p>trilaminar appearance</p> Signup and view all the answers

The central clear lamina in Robertson's trilaminar model is referred to as the ______ lamina.

<p>osmophobic</p> Signup and view all the answers

All membrane proteins are covalently bonded to the lipid bilayer.

<p>False (B)</p> Signup and view all the answers

Match the scientists with their contributions to cell membrane models:

<p>Gorter and Grindel = Lipid bilayer model without protein organization Danielli and Davson = Proposed proteins adsorbed at polar faces of membranes Robertson = Confirmed trilaminar appearance with osmium tetroxide Singer and Nicolson = Developed the fluid-mosaic model</p> Signup and view all the answers

How many times do single-pass polytopic membrane proteins cross the membrane?

<p>Once</p> Signup and view all the answers

Which of the following accurately describes the lipid bilayer model?

<p>It involves hydrophobic tails avoiding water. (B)</p> Signup and view all the answers

The average thickness of osmophilic lamina is greater than that of the osmophobic lamina.

<p>False (B)</p> Signup and view all the answers

What is the average total thickness of the trilaminar appearance in cell membranes?

<p>between 65 and 85 Ã…</p> Signup and view all the answers

What structure comprises the components of gap junctions?

<p>Connexins (B)</p> Signup and view all the answers

Synaptic junctions involve direct contact between presynaptic and postsynaptic membranes.

<p>False (B)</p> Signup and view all the answers

Name the neurotransmitter that is released at the synaptic junction.

<p>acetylcholine</p> Signup and view all the answers

Gap junctions are made up of six subunits of connexins, which form a structure called a ______.

<p>connexon</p> Signup and view all the answers

Match the following features with their correct descriptions:

<p>Synaptic Junctions = Contact points between neurons and other cells Gap Junctions = Aqueous channels between neighboring cells Cadherins = Members involved in synaptic connections Neurotransmitters = Chemicals released to transmit nerve impulses</p> Signup and view all the answers

Which statement about gap junctions is correct?

<p>They enable direct ion movement between cells. (D)</p> Signup and view all the answers

Gap junctions are present in nearly all tissues of the body.

<p>False (B)</p> Signup and view all the answers

What is the width range of the synaptic cleft?

<p>20 to 50 nm</p> Signup and view all the answers

What is the primary component of the primary cell wall in plants?

<p>Cellulose (B)</p> Signup and view all the answers

Connexins can open and close the gap junctions permanently.

<p>False (B)</p> Signup and view all the answers

What diameter range do polar molecules need to be in order to pass through gap junctions?

<p>less than 2 nm</p> Signup and view all the answers

The cytoplasmic bridges between adjacent plant cells for communication and nutrient exchange are known as __________.

<p>plasmodesmata</p> Signup and view all the answers

Match the following components to their functions in plant cell walls:

<p>Cellulose = Provides structural support Pectin = Mediates cell adhesion Lignin = Increases rigidity in secondary walls Hemicellulose = Stabilizes the cell wall matrix</p> Signup and view all the answers

What causes the opening of gap junctions?

<p>Decreased pH (C), Increase in calcium concentration (D)</p> Signup and view all the answers

Gap junctions allow for selective passage of small molecules.

<p>False (B)</p> Signup and view all the answers

What is formed when connexins assemble in gap junctions?

<p>a hydrophilic channel</p> Signup and view all the answers

Flashcards

What stabilizes lipid bilayers in cell membranes?

The main force stabilizing the lipid bilayer of cell membranes is the hydrophobic interaction between the hydrophobic tails of lipids, which avoids contact with water in the surrounding environment.

What did the Gorter-Grindel model fail to explain?

The Gorter-Grindel model of the cell membrane only addressed the lipid bilayer structure, neglecting the organization of proteins.

How did the Danielli-Davson model describe protein arrangement in the membrane?

The Danielli-Davson model proposed that proteins are adsorbed to the polar faces of the lipid bilayer and may span the membrane, forming channels.

What causes the trilaminar appearance of membranes under TEM?

The trilaminar appearance of membranes observed under Transmission Electron Microscopy (TEM) after staining with osmium tetroxide is due to the different affinities of the hydrophilic and hydrophobic regions of the lipid bilayer to osmium.

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Is cell membrane thickness constant across all cells?

The thickness of cell membranes is variable, depending on the composition of fatty acids and proteins.

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What is the 'fluid-mosaic model' of cell membrane?

The fluid-mosaic model describes the cell membrane as a fluid structure where proteins are embedded in a lipid bilayer, creating a mosaic-like appearance.

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Why is the fluid-mosaic model considered an improvement on previous models?

The fluid-mosaic model combines the understanding of both the lipid bilayer structure and protein distribution, providing a comprehensive description of cell membrane organization.

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What is the main implication of the fluid-mosaic model?

The fluid-mosaic model implies that membranes are dynamic and constantly in motion, allowing for flexibility and adaptation to various cellular functions.

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Fluid-Mosaic Model

A model describing the structure of cell membranes, where phospholipids form a bilayer with proteins embedded within, resembling a mosaic.

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Peripheral Protein

A type of protein that is loosely attached to the surface of the cell membrane, either on the inner or outer side.

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Integral Protein

A type of protein that is tightly bound to the cell membrane, often connected through a lipid molecule.

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Polytopic Protein

A type of integral protein that spans the entire cell membrane, passing through both layers.

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Monotopic Protein

A type of integral protein that only passes through one layer of the cell membrane.

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Hydrophobic Segment

A specific stretch of amino acids in integral proteins that is composed of hydrophobic residues. This region helps the protein anchor itself within the hydrophobic core of the membrane.

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Receptor Protein

A type of integral membrane protein that binds to a specific molecule, such as a hormone, and initiates a signal transduction pathway within the cell.

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Membrane-Associated Protein

A type of protein that associates with other proteins or cytoskeletal components, helping to maintain the structure and function of the cell membrane.

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Membrane Asymmetry

The unequal distribution of proteins, lipids, and carbohydrates between the inner and outer leaflets of a cell membrane.

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Flipases

Proteins that help transport lipids from one leaflet of the cell membrane to the other, maintaining membrane asymmetry.

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Extracellular Matrix-Interacting Proteins

Proteins that interact with the extracellular matrix, located on the outer leaflet of the cell membrane.

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Cytoskeleton-Interacting Proteins

Proteins that interact with the cytoskeleton, located on the inner leaflet of the cell membrane.

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Lipid Asymmetry

The unequal distribution of lipids between the inner and outer leaflets of a cell membrane.

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Carbohydrate Asymmetry

The presence of carbohydrate motifs always directed towards the exterior side of the plasma membrane.

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Membrane Fluidity

The property of cell membranes to be flexible and dynamic, allowing for movement of lipids and proteins within the membrane.

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Factors Affecting Membrane Fluidity

Factors like temperature, lipid composition, and cholesterol content affect the fluidity of cell membranes.

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Synaptic junctions

Specialized junctions between neurons and other cells (e.g., muscle fibers, gland cells, nerve cells). They involve recognition between presynaptic and postsynaptic membranes facilitated by cadherins.

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Synaptic cleft

A narrow space (20-50 nm) between the presynaptic and postsynaptic membranes at a synaptic junction. Neurotransmitters diffuse across this gap to transmit signals.

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Neurotransmitters

Chemical messengers released from the presynaptic neuron at a synaptic junction. Examples include acetylcholine.

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Receptors

Integral membrane proteins on the postsynaptic membrane that bind neurotransmitters, initiating a signal.

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Gap junctions

Clusters of pipeline-like structures between neighboring cells, allowing the exchange of molecules and solutes.

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Connexins

Integral proteins that form the gap junctions and facilitate the passage of molecules.

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Connexon

Clusters of six connexin subunits that form the core of a gap junction channel.

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Peristaltic movement

A movement of the esophagus that occurs due to coordinated contractions of smooth muscle facilitated by gap junctions, enabling swallowing.

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Heterokaryon

A hybrid cell formed by the fusion of two cells from different species, often containing distinct markers for identification.

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Fluorescence Recovery After Photobleaching (FRAP)

A technique used to track the movement of molecules within a cell membrane by selectively irradiating a region and observing the recovery of fluorescence.

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Lateral Diffusion

The ability of molecules, like proteins and lipids, to move laterally within the plane of a cell membrane.

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Cytoskeleton

The structural network within a cell that provides support and helps organize the movement of molecules and organelles.

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Membrane Domains

Specialized cell structures that are anchored to the cytoskeleton, influencing the movement of transmembrane proteins.

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Polarized Plasma Membrane

The process where specialized cells, like epithelial cells, have distinct membrane regions with different functions and protein composition.

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Cytoskeleton-Extracellular Matrix Interaction

The interaction between the cell's internal cytoskeleton and the extracellular matrix, affecting the mobility of membrane proteins.

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What are gap junctions?

A specialized cell junction found in animal cells, formed by the close apposition of plasma membranes from adjacent cells, allowing the passage of small molecules and ions.

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What are connexons?

Connexons are protein channels that form gap junctions. Each connexon is composed of six connexin proteins. The arrangement of these proteins allows small molecules to pass between cells.

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Are gap junctions selective?

The passage of molecules through gap junctions is not selective. This means that small molecules such as amino acids and sugars can pass through easily. However, the passage of larger molecules is restricted.

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What controls the communication through gap junctions?

The opening and closing of gap junctions is regulated by various factors. For example, a decrease in pH or an increase in calcium concentration can cause the gap junction to close.

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What is a plant cell wall?

A rigid structure that surrounds plant cells, composed primarily of cellulose and a matrix of hemicellulose, pectin, and glycoproteins.

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What are plasmodesmata?

Plasmodesmata are channels that connect the cytoplasm of adjacent plant cells, facilitating the exchange of molecules and communication between cells.

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How does the cell wall relate to plasmodesmata?

The cell wall is interrupted at the location of plasmodesmata, allowing the plasma membranes of adjacent cells to connect.

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What is a desmotubule?

A membranous structure found in the center of a plasmodesma, derived from the smooth endoplasmic reticulum (SER), which may help regulate the passage of molecules.

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Study Notes

Cell Membranes

  • Cell membranes, also called biomembranes, are found in all cells.
  • These membranes include the plasma membrane, nuclear membrane, endoplasmic reticulum (ER) membrane, Golgi apparatus membrane, vacuole membrane, and mitochondrial membranes.
  • Cytomembranes separate specific compartments within cells from their surroundings.
  • Prokaryotes have less complex compartmentalization than eukaryotes.
  • Plasma membranes are crucial for maintaining cell integrity.
  • Damage to the plasma membrane can lead to cell death.
  • Cell membranes perform various biochemical reactions and physiological processes, including signal transduction, phosphorylation, and respiration (prokaryotes).
  • Cells communicate and interact with neighboring cells via their plasma membranes.
  • All cell membranes have selective permeability.
  • They control the passage of substances between compartments.
  • Some molecules pass freely, while others require channels or transporters.

Lipid Bilayer

  • The primary structure of cell membranes is a lipid bilayer.
  • This is composed of phospholipids (glycerol-derived and sphingosine-derived), glycolipids, and steroids (e.g., cholesterol).
  • Phospholipids arrange themselves in a bilayer to avoid contact between hydrophobic tails and water.
  • This arrangement is stabilized by hydrophobic interactions.

Fluid Mosaic Model

  • The fluid mosaic model describes membrane structure and properties.
  • The model suggests that a membrane consists of a phospholipid bilayer with proteins embedded in a mosaic-like pattern.

Types of Membrane Proteins

  • Peripheral or Extrinsic Proteins: Weakly attached to the lipid bilayer's inner or outer surfaces. Often involved in adhesion (e.g., to cytoskeleton or other proteins).
  • Integral or Intrinsic Proteins: Strongly attached to the lipid bilayer. Can span the membrane completely (transmembrane) or partially (monotopic).

Membrane Asymmetry

  • Protein and lipid distribution across the lipid bilayer is not uniform.
  • The outer and inner leaflets (layers) differ in composition.
  • This asymmetry is crucial for membrane function.

Membrane Fluidity

  • Membranes are dynamic structures; molecules are constantly moving.
  • Fluidity is affected by factors like temperature, fatty acid composition (saturation), and presence of cholesterol.
  • Cholesterol moderates membrane fluidity at both high and low temperatures.

Membrane Polarization

  • Cell membranes frequently have specialized regions with different properties and functions.
  • Examples include apical and basal domains in polarized cells (e.g., epithelial cells).

Cell Junctions

  • Tight junctions: Seal adjacent cells together.
  • Intermediate junctions (zonula adherens): Link cells via intracellular filaments (e.g., actin).
  • Desmosomes (macula adherens): Anchor cells with strong connections.
  • Gap junctions (communicating junctions): Allow small molecules and ions to pass between cells.

Synaptic Junctions

  • These are specialized cell-cell contacts between neurons which transmit signals (neurotransmitters).

Microdomains

  • Lipid rafts are specialized regions within the cell membrane, rich in specific lipids and proteins.

Plant Cell Walls and Plasmodesmata

  • Plant cells have a rigid cell wall outside the plasma membrane.
  • Plasmodesmata are intercellular channels that connect plant cells, allowing cell-cell communication and transport.

Transport of Macromolecules

  • Endocytosis, exocytosis, and phagocytosis are mechanisms for transporting large molecules in and out of the cell.

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