Cell Growth Models and Kinetics in Biochemical Engineering
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Questions and Answers

What is the purpose of cell quantification in biochemical engineering?

  • To monitor bioprocesses in-line
  • To perform direct cell counting
  • To understand and model biological systems
  • To predict and optimize bioprocess performance (correct)

Which technology aims to monitor bioprocesses either in-line or off-line?

  • Hemocytometer
  • Process analytical technology (correct)
  • Bioprocessing
  • Cell quantification

What is a common way to perform direct cell counting?

  • Bioprocessing
  • Cell quantification
  • Hemocytometer (correct)
  • Process analytical technology

Which fundamental tools are provided in the chapter to model cell growth and product formation?

<p>Quantitative methods and cell growth models (C)</p> Signup and view all the answers

What are the environmental conditions that affect cell growth described as?

<p>Optimizing bioprocess performance (C)</p> Signup and view all the answers

What does biochemical engineering seek to understand and model using quantitative methods?

<p>Biological systems (B)</p> Signup and view all the answers

What is the purpose of using stains in cell quantification?

<p>To determine the viability of the cells (A)</p> Signup and view all the answers

Which method is suitable for non-aggregated cultures where individual cells are easily distinguishable by eye or image analysis?

<p>Colony formation assay (C)</p> Signup and view all the answers

What is correlated with particle size in flow cytometry?

<p>Forward scatter (C)</p> Signup and view all the answers

What is correlated with particle granularity in flow cytometry?

<p>Side scatter (B)</p> Signup and view all the answers

In CFU counting, what does CFU stand for?

<p>Colony Formation Units (A)</p> Signup and view all the answers

What is the purpose of using an automated system where cells pass through a small channel in cell quantification?

<p>To measure changes in light scattering (C)</p> Signup and view all the answers

What do stains help to determine in the cell quantification process?

<p>Viability of the cells (A)</p> Signup and view all the answers

What is a common apparatus used for particle enumeration and fluorescence measurement?

<p>Coulter counter (C)</p> Signup and view all the answers

What method is commonly used to quantify mammalian stem or progenitor cells?

<p>Colony formation assay (A)</p> Signup and view all the answers

What do culture samples undergo before being spread on Petri dishes in colony formation assays?

<p>Dilution (D)</p> Signup and view all the answers

What is the purpose of using a hemocytometer in cell quantification?

<p>To quantify non-aggregated cultures where individual cells are easily distinguishable by eye or image analysis (C)</p> Signup and view all the answers

In biochemical engineering, what technology is commonly used to monitor bioprocesses in-line or off-line?

<p>Automated system with small channel for cell passage (A)</p> Signup and view all the answers

What tool is commonly used for particle enumeration and fluorescence measurement in biochemical engineering?

<p>Flow cytometry (D)</p> Signup and view all the answers

What fundamental tools are provided in the chapter to model cell growth and product formation in bioprocessing?

<p>Cell growth models and product formation assays (B)</p> Signup and view all the answers

What environmental conditions affect cell growth in biochemical engineering?

<p>Biomass and metabolic heat (C)</p> Signup and view all the answers

What is correlated with particle size in flow cytometry?

<p>Particle granularity (D)</p> Signup and view all the answers

What is the purpose of using a hemocytometer in cell quantification?

<p>To create a small chamber for placing the sample and counting cells (B)</p> Signup and view all the answers

What method is commonly used to quantify mammalian stem or progenitor cells?

<p>Spread on Petri dishes with appropriate cell culture surfaces (B)</p> Signup and view all the answers

What environmental conditions affect cell growth in biochemical engineering?

<p>Temperature, pH, and nutrient availability (B)</p> Signup and view all the answers

What is correlated with particle size in flow cytometry?

<p>Light scattering (B)</p> Signup and view all the answers

What do stains help to determine in the cell quantification process?

<p>The viability of the cells (A)</p> Signup and view all the answers

What does CFU stand for in CFU counting method?

<p>Colony-Forming Units (A)</p> Signup and view all the answers

What is a common apparatus used for particle enumeration and fluorescence measurement?

<p>Flow cytometer (A)</p> Signup and view all the answers

What technology aims to monitor bioprocesses either in-line or off-line?

<p><strong>Flow cytometer</strong> (D)</p> Signup and view all the answers

What is correlated with particle granularity in flow cytometry?

<p>Blockage of light by the particle such as cells (B)</p> Signup and view all the answers

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