Cell Growth Models and Kinetics in Biochemical Engineering
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Questions and Answers

What is the purpose of cell quantification in biochemical engineering?

  • To monitor bioprocesses in-line
  • To perform direct cell counting
  • To understand and model biological systems
  • To predict and optimize bioprocess performance (correct)

    • Which technology aims to monitor bioprocesses either in-line or off-line?

  • Hemocytometer
  • Process analytical technology (correct)
  • Bioprocessing
  • Cell quantification

    • What is a common way to perform direct cell counting?

  • Bioprocessing
  • Cell quantification
  • Hemocytometer (correct)
  • Process analytical technology

    • Which fundamental tools are provided in the chapter to model cell growth and product formation?

    <p>Quantitative methods and cell growth models</p> Signup and view all the answers

    What are the environmental conditions that affect cell growth described as?

    <p>Optimizing bioprocess performance</p> Signup and view all the answers

    What does biochemical engineering seek to understand and model using quantitative methods?

    <p>Biological systems</p> Signup and view all the answers

    What is the purpose of using stains in cell quantification?

    <p>To determine the viability of the cells</p> Signup and view all the answers

    Which method is suitable for non-aggregated cultures where individual cells are easily distinguishable by eye or image analysis?

    <p>Colony formation assay</p> Signup and view all the answers

    What is correlated with particle size in flow cytometry?

    <p>Forward scatter</p> Signup and view all the answers

    What is correlated with particle granularity in flow cytometry?

    <p>Side scatter</p> Signup and view all the answers

    In CFU counting, what does CFU stand for?

    <p>Colony Formation Units</p> Signup and view all the answers

    What is the purpose of using an automated system where cells pass through a small channel in cell quantification?

    <p>To measure changes in light scattering</p> Signup and view all the answers

    What do stains help to determine in the cell quantification process?

    <p>Viability of the cells</p> Signup and view all the answers

    What is a common apparatus used for particle enumeration and fluorescence measurement?

    <p>Coulter counter</p> Signup and view all the answers

    What method is commonly used to quantify mammalian stem or progenitor cells?

    <p>Colony formation assay</p> Signup and view all the answers

    What do culture samples undergo before being spread on Petri dishes in colony formation assays?

    <p>Dilution</p> Signup and view all the answers

    What is the purpose of using a hemocytometer in cell quantification?

    <p>To quantify non-aggregated cultures where individual cells are easily distinguishable by eye or image analysis</p> Signup and view all the answers

    In biochemical engineering, what technology is commonly used to monitor bioprocesses in-line or off-line?

    <p>Automated system with small channel for cell passage</p> Signup and view all the answers

    What tool is commonly used for particle enumeration and fluorescence measurement in biochemical engineering?

    <p>Flow cytometry</p> Signup and view all the answers

    What fundamental tools are provided in the chapter to model cell growth and product formation in bioprocessing?

    <p>Cell growth models and product formation assays</p> Signup and view all the answers

    What environmental conditions affect cell growth in biochemical engineering?

    <p>Biomass and metabolic heat</p> Signup and view all the answers

    What is correlated with particle size in flow cytometry?

    <p>Particle granularity</p> Signup and view all the answers

    What is the purpose of using a hemocytometer in cell quantification?

    <p>To create a small chamber for placing the sample and counting cells</p> Signup and view all the answers

    What method is commonly used to quantify mammalian stem or progenitor cells?

    <p>Spread on Petri dishes with appropriate cell culture surfaces</p> Signup and view all the answers

    What environmental conditions affect cell growth in biochemical engineering?

    <p>Temperature, pH, and nutrient availability</p> Signup and view all the answers

    What is correlated with particle size in flow cytometry?

    <p>Light scattering</p> Signup and view all the answers

    What do stains help to determine in the cell quantification process?

    <p>The viability of the cells</p> Signup and view all the answers

    What does CFU stand for in CFU counting method?

    <p>Colony-Forming Units</p> Signup and view all the answers

    What is a common apparatus used for particle enumeration and fluorescence measurement?

    <p>Flow cytometer</p> Signup and view all the answers

    What technology aims to monitor bioprocesses either in-line or off-line?

    <p><strong>Flow cytometer</strong></p> Signup and view all the answers

    What is correlated with particle granularity in flow cytometry?

    <p>Blockage of light by the particle such as cells</p> Signup and view all the answers

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