31 Questions
What is the purpose of cell quantification in biochemical engineering?
To predict and optimize bioprocess performance
Which technology aims to monitor bioprocesses either in-line or off-line?
Process analytical technology
What is a common way to perform direct cell counting?
Hemocytometer
Which fundamental tools are provided in the chapter to model cell growth and product formation?
Quantitative methods and cell growth models
What are the environmental conditions that affect cell growth described as?
Optimizing bioprocess performance
What does biochemical engineering seek to understand and model using quantitative methods?
Biological systems
What is the purpose of using stains in cell quantification?
To determine the viability of the cells
Which method is suitable for non-aggregated cultures where individual cells are easily distinguishable by eye or image analysis?
Colony formation assay
What is correlated with particle size in flow cytometry?
Forward scatter
What is correlated with particle granularity in flow cytometry?
Side scatter
In CFU counting, what does CFU stand for?
Colony Formation Units
What is the purpose of using an automated system where cells pass through a small channel in cell quantification?
To measure changes in light scattering
What do stains help to determine in the cell quantification process?
Viability of the cells
What is a common apparatus used for particle enumeration and fluorescence measurement?
Coulter counter
What method is commonly used to quantify mammalian stem or progenitor cells?
Colony formation assay
What do culture samples undergo before being spread on Petri dishes in colony formation assays?
Dilution
What is the purpose of using a hemocytometer in cell quantification?
To quantify non-aggregated cultures where individual cells are easily distinguishable by eye or image analysis
In biochemical engineering, what technology is commonly used to monitor bioprocesses in-line or off-line?
Automated system with small channel for cell passage
What tool is commonly used for particle enumeration and fluorescence measurement in biochemical engineering?
Flow cytometry
What fundamental tools are provided in the chapter to model cell growth and product formation in bioprocessing?
Cell growth models and product formation assays
What environmental conditions affect cell growth in biochemical engineering?
Biomass and metabolic heat
What is correlated with particle size in flow cytometry?
Particle granularity
What is the purpose of using a hemocytometer in cell quantification?
To create a small chamber for placing the sample and counting cells
What method is commonly used to quantify mammalian stem or progenitor cells?
Spread on Petri dishes with appropriate cell culture surfaces
What environmental conditions affect cell growth in biochemical engineering?
Temperature, pH, and nutrient availability
What is correlated with particle size in flow cytometry?
Light scattering
What do stains help to determine in the cell quantification process?
The viability of the cells
What does CFU stand for in CFU counting method?
Colony-Forming Units
What is a common apparatus used for particle enumeration and fluorescence measurement?
Flow cytometer
What technology aims to monitor bioprocesses either in-line or off-line?
Flow cytometer
What is correlated with particle granularity in flow cytometry?
Blockage of light by the particle such as cells
Test your knowledge on cell growth models, kinetics, and yields in biochemical engineering. Learn about the phases of batch cell growth, methods to quantify cells, interpretation of yields and rates, prediction of cell concentration based on growth models, and the relationship between biomass and metabolic heat.
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