Podcast
Questions and Answers
Photobioreactors are used to grow small phototrophic organisms such as cyanobacteria, algae, or ______.
Photobioreactors are used to grow small phototrophic organisms such as cyanobacteria, algae, or ______.
moss
These organisms use light through ______ as their energy source.
These organisms use light through ______ as their energy source.
photosynthesis
In conventional sewage treatment, bioreactors are utilized to undertake the main purification processes in ______.
In conventional sewage treatment, bioreactors are utilized to undertake the main purification processes in ______.
sewage
The separation of excess biological film takes place in settling tanks or ______.
The separation of excess biological film takes place in settling tanks or ______.
An extremely simple version of a sewage bioreactor is a ______ tank.
An extremely simple version of a sewage bioreactor is a ______ tank.
Many research groups have developed novel bioreactors for growing specialized ______ and cells.
Many research groups have developed novel bioreactors for growing specialized ______ and cells.
Currently, scaling production of specialized bioreactors for ______ use remains challenging.
Currently, scaling production of specialized bioreactors for ______ use remains challenging.
A biosafety cabinet is also known as a biological safety ______.
A biosafety cabinet is also known as a biological safety ______.
The acronym for biosafety cabinet is ______.
The acronym for biosafety cabinet is ______.
Biosafety cabinets are primarily used for ______ protection.
Biosafety cabinets are primarily used for ______ protection.
All exhaust air from a biosafety cabinet is ______-filtered.
All exhaust air from a biosafety cabinet is ______-filtered.
A ______ sequencer is used to automate the DNA sequencing process.
A ______ sequencer is used to automate the DNA sequencing process.
The four bases in DNA are G, C, A, and ______.
The four bases in DNA are G, C, A, and ______.
The Sanger sequencing method was developed in ______.
The Sanger sequencing method was developed in ______.
Most models of biosafety cabinets aim to maintain the sterility of materials inside the ______.
Most models of biosafety cabinets aim to maintain the sterility of materials inside the ______.
Biosafety cabinets differ from laminar flow clean benches in that they provide ______ protection.
Biosafety cabinets differ from laminar flow clean benches in that they provide ______ protection.
A cleanroom maintains a very low concentration of airborne ______.
A cleanroom maintains a very low concentration of airborne ______.
In US climates, a typical fume hood uses 3.5 times as much energy as a ______.
In US climates, a typical fume hood uses 3.5 times as much energy as a ______.
Cleanrooms are commonly used in scientific research and ______ production.
Cleanrooms are commonly used in scientific research and ______ production.
In a cleanroom, outside air is filtered and cooled by outdoor air ______.
In a cleanroom, outside air is filtered and cooled by outdoor air ______.
The primary aim of a cleanroom can be to prevent hazardous materials from ______.
The primary aim of a cleanroom can be to prevent hazardous materials from ______.
An enclosed glovebox completely isolates the operator from all direct physical contact with the work material and tools, especially when handling ______ materials.
An enclosed glovebox completely isolates the operator from all direct physical contact with the work material and tools, especially when handling ______ materials.
Most fume hoods are fitted with a mains-powered control ______.
Most fume hoods are fitted with a mains-powered control ______.
The front of a glovebox generally consists of a sash window that can move up and down on a ______ mechanism.
The front of a glovebox generally consists of a sash window that can move up and down on a ______ mechanism.
Recirculation of lab air is not permitted; instead, air handling units serving the non-laboratory areas are kept ______ from the laboratory units.
Recirculation of lab air is not permitted; instead, air handling units serving the non-laboratory areas are kept ______ from the laboratory units.
To improve indoor air quality, some laboratories utilize single-pass air handling ______ systems.
To improve indoor air quality, some laboratories utilize single-pass air handling ______ systems.
A 'high sash' alarm is caused by the sliding glass being raised higher than is considered ______.
A 'high sash' alarm is caused by the sliding glass being raised higher than is considered ______.
Many labs have fume hoods that draw conditioned air from the lab space and disperse it into the outside ______.
Many labs have fume hoods that draw conditioned air from the lab space and disperse it into the outside ______.
To minimize energy and running costs, laboratories continue to use return air systems to the laboratory areas while still providing adequate ______ rates.
To minimize energy and running costs, laboratories continue to use return air systems to the laboratory areas while still providing adequate ______ rates.
Variable air volume (VAV) systems are employed to reduce the volume of the air ______ as the fume hood sash is closed.
Variable air volume (VAV) systems are employed to reduce the volume of the air ______ as the fume hood sash is closed.
Ducted fume hoods exist to draw heated or cooled air from the lab space into the fume hood and then disperse it via ______ into the outside atmosphere.
Ducted fume hoods exist to draw heated or cooled air from the lab space into the fume hood and then disperse it via ______ into the outside atmosphere.
A fume hood is designed to limit exposure to hazardous or toxic ______, vapors or dusts.
A fume hood is designed to limit exposure to hazardous or toxic ______, vapors or dusts.
The two main types of fume hoods are ducted and ______.
The two main types of fume hoods are ducted and ______.
Fume hoods help protect the user from inhaling toxic ______.
Fume hoods help protect the user from inhaling toxic ______.
A common modern fume hood encloses ______ sides of a work area.
A common modern fume hood encloses ______ sides of a work area.
The principle of fume hoods is that air is drawn in from the front ______ side of the cabinet.
The principle of fume hoods is that air is drawn in from the front ______ side of the cabinet.
Fume hoods can also protect the ______ or experiment from contamination.
Fume hoods can also protect the ______ or experiment from contamination.
Secondary functions of fume hoods may include explosion protection and spill ______.
Secondary functions of fume hoods may include explosion protection and spill ______.
Fume hoods are often fitted with infills above to cover up the exhaust ______.
Fume hoods are often fitted with infills above to cover up the exhaust ______.
DNA sequencers can be used in the genotyping of genetic ______ where only the length needs to be determined.
DNA sequencers can be used in the genotyping of genetic ______ where only the length needs to be determined.
Comparing data quality and cost across different sequencing ______ can be a daunting task.
Comparing data quality and cost across different sequencing ______ can be a daunting task.
Flashcards
What are photobioreactors used for?
What are photobioreactors used for?
Photobioreactors are transparent containers used to cultivate photosynthetic organisms like algae and cyanobacteria. These organisms use light for energy, reducing the risk of contamination by bacteria or fungi compared to traditional bioreactors.
How does sewage treatment use bioreactors?
How does sewage treatment use bioreactors?
Sewage treatment often involves bioreactors, where microorganisms break down waste, purifying the water. These bioreactors can contain inert media for biological film growth, or they can be aerated to promote 'activated sludge' formation.
What is the function of 'biological film' in bioreactors?
What is the function of 'biological film' in bioreactors?
In some bioreactors, a surface with a lot of space (high surface area) is provided for microbes to grow on. This is called 'biological film'. This film acts like a filter, cleaning the water.
What is 'activated sludge' in sewage treatment?
What is 'activated sludge' in sewage treatment?
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How do bioreactors work for cleaning sewage?
How do bioreactors work for cleaning sewage?
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What is a septic tank and how does it work?
What is a septic tank and how does it work?
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What is a tissue bioreactor and what is it used for?
What is a tissue bioreactor and what is it used for?
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Biosafety Cabinet (BSC)
Biosafety Cabinet (BSC)
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What type of filtration does a Biosafety Cabinet use?
What type of filtration does a Biosafety Cabinet use?
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Biocontainment
Biocontainment
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DNA Sequencer
DNA Sequencer
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DNA 'Read'
DNA 'Read'
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Fluorochromes
Fluorochromes
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First Generation DNA Sequencers
First Generation DNA Sequencers
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Sanger Sequencing
Sanger Sequencing
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How do first generation DNA sequencers work?
How do first generation DNA sequencers work?
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What is a cleanroom?
What is a cleanroom?
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How do fume hoods save energy?
How do fume hoods save energy?
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What is the purpose of a cleanroom?
What is the purpose of a cleanroom?
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What are HEPA and ULPA filters used for in cleanrooms?
What are HEPA and ULPA filters used for in cleanrooms?
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How are cleanrooms designed to minimize particle generation?
How are cleanrooms designed to minimize particle generation?
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DNA sequence assembly
DNA sequence assembly
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Fume hood
Fume hood
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Short-read sequencers
Short-read sequencers
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Biosafety cabinet
Biosafety cabinet
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Glove box
Glove box
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Recirculating fume hood
Recirculating fume hood
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Genotyping
Genotyping
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Ducted fume hood
Ducted fume hood
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Genome sequencing
Genome sequencing
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DNA fragment length
DNA fragment length
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Fume hood sash
Fume hood sash
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High sash alarm
High sash alarm
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Single-pass air handling system
Single-pass air handling system
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Return air system
Return air system
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Variable air volume (VAV) system
Variable air volume (VAV) system
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Control panel
Control panel
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Low airflow alarm
Low airflow alarm
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Enclosed glovebox
Enclosed glovebox
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Study Notes
Bioreactors
- Bioreactors are manufactured devices supporting biologically active environments.
- They can be vessels for chemical processes involving organisms or biochemically active substances.
- Processes can be aerobic or anaerobic.
- Bioreactors are typically cylindrical, ranging from liters to cubic meters.
- They're commonly made of stainless steel.
- Can also refer to devices for growing cells or tissues in cell culture (e.g., tissue engineering, biochemical/bioprocess engineering).
- Classified as batch, fed-batch, or continuous (e.g., continuous stirred-tank reactor, chemostat) based on operation mode.
- Bioreactors can support submerged cultures (organisms suspended in liquid) or immobilized cultures (organisms anchored to a solid medium).
Continuous Stirred-Tank Bioreactor
- A continuous bioreactor type.
- Organisms/substances are commonly in a liquid medium.
- Organisms can be suspended or immobilized; immobilization requires no specialized attachment surfaces.
- Continuous operation allows for larger scale operation than immobilized cultures.
- Organisms are removed from the reactor continuously.
Bioreactor Design
- A complex engineering task.
- Environmental conditions (temperature, nutrient concentration, pH, dissolved gases, e.g., oxygen for aerobic fermentations) affect organism growth and productivity.
- Maintaining optimal temperature may require cooling jackets, coils or external heat exchangers.
- Nutrients may be added continuously (fed-batch) or charged at the start of fermentation.
- pH is adjusted based on process needs.
- Oxygen addition often required for aerobic fermentations; oxygen is difficult to add due to low solubility in water, leading to pressurized systems (e.g., for increasing solubility).
- Cleaning is important to prevent fouling.
Photobioreactors (PBRs)
- Incorporate light sources (natural or artificial).
- Used for growing phototrophic microorganisms (e.g., cyanobacteria, algae, moss plants).
- Photosynthesis is the primary energy source.
- Generally, less risk of contamination from heterotrophic organisms compared to other bioreactors.
Sewage Treatment
- Bioreactors are vital for purifying sewage.
- In some systems, chemically inert mediums provide substrates for biological film growth.
- Excess biological film is separated, usually in settling tanks.
Biosafety Cabinets (BSCs)
- Enclosed, ventilated laboratory workspaces for safe handling of materials, especially if contaminated with pathogens.
- Different types, varying in biocontainment.
- Primarily protect the user and environment by HEPA-filtering exhaust air, removing harmful bacteria and viruses.
- Secondary functions may include preventing spills, explosion protection.
DNA Sequencers
- Scientific instruments for automating DNA sequencing.
- Determine the order of DNA bases (G, C, A, T).
- Often utilize optical instruments (detecting signals from fluorochromes attached to nucleotides).
- The Sanger sequencing method, which formed the basis of the first generation of DNA sequencers, was used in the human genome project.
Fume Hoods
- Local ventilation devices limiting exposure to hazardous gases, vapors, and dusts.
- Two main types: ducted (exhausts gas outside the facility) and recirculating (filters air).
- Contain a sash window to control air flow.
- Control panels regulate various functions including internal lighting, air flow warnings, and fan control.
Standard Glove Boxes
- Used for hazardous materials, isolating the user, maintaining negative pressure (or controlled pressure) to prevent leaks.
Cleanrooms
- Engineered spaces with low concentrations of airborne particulates, for e.g. semiconductors, scientific research.
- Controlled humidity and temperature.
- Filtered air using HEPA and ULPA filters.
- Carefully chosen materials to minimize airborne particle generation.
Personnel Contamination of Cleanrooms
- Users are the primary source of contamination in cleanroom environments
- Controlling microbial contamination is crucial.
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