Bioreactors Overview Quiz

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Questions and Answers

Photobioreactors are used to grow small phototrophic organisms such as cyanobacteria, algae, or ______.

moss

These organisms use light through ______ as their energy source.

photosynthesis

In conventional sewage treatment, bioreactors are utilized to undertake the main purification processes in ______.

sewage

The separation of excess biological film takes place in settling tanks or ______.

<p>cyclones</p> Signup and view all the answers

An extremely simple version of a sewage bioreactor is a ______ tank.

<p>septic</p> Signup and view all the answers

Many research groups have developed novel bioreactors for growing specialized ______ and cells.

<p>tissues</p> Signup and view all the answers

Currently, scaling production of specialized bioreactors for ______ use remains challenging.

<p>industrial</p> Signup and view all the answers

A biosafety cabinet is also known as a biological safety ______.

<p>cabinet</p> Signup and view all the answers

The acronym for biosafety cabinet is ______.

<p>BSC</p> Signup and view all the answers

Biosafety cabinets are primarily used for ______ protection.

<p>worker</p> Signup and view all the answers

All exhaust air from a biosafety cabinet is ______-filtered.

<p>HEPA</p> Signup and view all the answers

A ______ sequencer is used to automate the DNA sequencing process.

<p>DNA</p> Signup and view all the answers

The four bases in DNA are G, C, A, and ______.

<p>T</p> Signup and view all the answers

The Sanger sequencing method was developed in ______.

<p>2001</p> Signup and view all the answers

Most models of biosafety cabinets aim to maintain the sterility of materials inside the ______.

<p>product</p> Signup and view all the answers

Biosafety cabinets differ from laminar flow clean benches in that they provide ______ protection.

<p>environmental</p> Signup and view all the answers

A cleanroom maintains a very low concentration of airborne ______.

<p>particulates</p> Signup and view all the answers

In US climates, a typical fume hood uses 3.5 times as much energy as a ______.

<p>home</p> Signup and view all the answers

Cleanrooms are commonly used in scientific research and ______ production.

<p>industrial</p> Signup and view all the answers

In a cleanroom, outside air is filtered and cooled by outdoor air ______.

<p>handlers</p> Signup and view all the answers

The primary aim of a cleanroom can be to prevent hazardous materials from ______.

<p>escaping</p> Signup and view all the answers

An enclosed glovebox completely isolates the operator from all direct physical contact with the work material and tools, especially when handling ______ materials.

<p>hazardous</p> Signup and view all the answers

Most fume hoods are fitted with a mains-powered control ______.

<p>panel</p> Signup and view all the answers

The front of a glovebox generally consists of a sash window that can move up and down on a ______ mechanism.

<p>counterbalance</p> Signup and view all the answers

Recirculation of lab air is not permitted; instead, air handling units serving the non-laboratory areas are kept ______ from the laboratory units.

<p>segregated</p> Signup and view all the answers

To improve indoor air quality, some laboratories utilize single-pass air handling ______ systems.

<p>air</p> Signup and view all the answers

A 'high sash' alarm is caused by the sliding glass being raised higher than is considered ______.

<p>safe</p> Signup and view all the answers

Many labs have fume hoods that draw conditioned air from the lab space and disperse it into the outside ______.

<p>atmosphere</p> Signup and view all the answers

To minimize energy and running costs, laboratories continue to use return air systems to the laboratory areas while still providing adequate ______ rates.

<p>ventilation</p> Signup and view all the answers

Variable air volume (VAV) systems are employed to reduce the volume of the air ______ as the fume hood sash is closed.

<p>exhausted</p> Signup and view all the answers

Ducted fume hoods exist to draw heated or cooled air from the lab space into the fume hood and then disperse it via ______ into the outside atmosphere.

<p>ducts</p> Signup and view all the answers

A fume hood is designed to limit exposure to hazardous or toxic ______, vapors or dusts.

<p>fumes</p> Signup and view all the answers

The two main types of fume hoods are ducted and ______.

<p>recirculating</p> Signup and view all the answers

Fume hoods help protect the user from inhaling toxic ______.

<p>gases</p> Signup and view all the answers

A common modern fume hood encloses ______ sides of a work area.

<p>five</p> Signup and view all the answers

The principle of fume hoods is that air is drawn in from the front ______ side of the cabinet.

<p>open</p> Signup and view all the answers

Fume hoods can also protect the ______ or experiment from contamination.

<p>product</p> Signup and view all the answers

Secondary functions of fume hoods may include explosion protection and spill ______.

<p>containment</p> Signup and view all the answers

Fume hoods are often fitted with infills above to cover up the exhaust ______.

<p>ductwork</p> Signup and view all the answers

DNA sequencers can be used in the genotyping of genetic ______ where only the length needs to be determined.

<p>markers</p> Signup and view all the answers

Comparing data quality and cost across different sequencing ______ can be a daunting task.

<p>technologies</p> Signup and view all the answers

Flashcards

What are photobioreactors used for?

Photobioreactors are transparent containers used to cultivate photosynthetic organisms like algae and cyanobacteria. These organisms use light for energy, reducing the risk of contamination by bacteria or fungi compared to traditional bioreactors.

How does sewage treatment use bioreactors?

Sewage treatment often involves bioreactors, where microorganisms break down waste, purifying the water. These bioreactors can contain inert media for biological film growth, or they can be aerated to promote 'activated sludge' formation.

What is the function of 'biological film' in bioreactors?

In some bioreactors, a surface with a lot of space (high surface area) is provided for microbes to grow on. This is called 'biological film'. This film acts like a filter, cleaning the water.

What is 'activated sludge' in sewage treatment?

To promote the growth of microbes in sewage treatment, some bioreactors use 'activated sludge'. This is a mixture of microbes and water that helps break down waste faster.

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How do bioreactors work for cleaning sewage?

This process uses microbes to break down waste in a contained space, often with the help of aeration or special media for the microbes to grow on. This removes pollutants and improves water quality.

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What is a septic tank and how does it work?

Septic tanks are simpler bioreactors where sewage is left to decompose, with or without added media to house bacteria. The sludge in the tank acts as a host for the microbes.

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What is a tissue bioreactor and what is it used for?

Researchers are developing bioreactors for growing specific tissues and cells on a scaffold, mimicking organ structures in a lab. This is called tissue engineering.

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Biosafety Cabinet (BSC)

A specialized enclosed workspace in a lab designed to protect workers and the environment from hazardous materials, typically containing pathogens.

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What type of filtration does a Biosafety Cabinet use?

A type of safety cabinet that uses a high-efficiency particulate air (HEPA) filter to remove harmful bacteria and viruses from the exhaust air. It provides protection for the worker, the environment, and the materials inside.

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Biocontainment

The ability of a biosafety cabinet to contain infectious agents, preventing their release into the environment and protecting the worker.

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DNA Sequencer

A scientific instrument used to determine the order of the four nitrogenous bases (G, C, A, T) in a DNA molecule. It outputs a series of letters representing the sequence.

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DNA 'Read'

The order of the bases (G, C, A, T) in a DNA molecule as determined by a DNA sequencer, presented as a string of letters.

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Fluorochromes

Fluorescent markers attached to nucleotides used in DNA sequencing to visualize the sequence during the process.

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First Generation DNA Sequencers

The first generation of DNA sequencers developed in the early 2000s, based on the Sanger Sequencing method, which enabled the completion of the Human Genome Project.

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Sanger Sequencing

A method used to determine the sequence of DNA by selectively terminating DNA replication, separating the fragments by size, and reading the sequence based on the order of fragments.

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How do first generation DNA sequencers work?

Automated electrophoresis systems that separate DNA fragments based on size and label them with fluorescent markers.

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What is a cleanroom?

A controlled environment with extremely low levels of airborne particles, used for sensitive processes like semiconductor manufacturing, pharmaceutical production, and scientific research.

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How do fume hoods save energy?

Fume hoods are designed to minimize the exhaust volume when not in use, reducing energy consumption and environmental impact.

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What is the purpose of a cleanroom?

Cleanrooms are designed to keep contaminants out and prevent materials from escaping, ensuring a controlled environment.

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What are HEPA and ULPA filters used for in cleanrooms?

Cleanrooms employ high-efficiency particulate absorbing (HEPA) and ultra-low particulate air (ULPA) filters to remove airborne contaminants.

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How are cleanrooms designed to minimize particle generation?

To minimize particle generation, cleanrooms utilize specialized lighting fixtures, walls, equipment, and materials.

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DNA sequence assembly

The process of combining short DNA sequences (reads) into longer contiguous sequences (contigs).

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Fume hood

A type of local ventilation device designed to protect users from hazardous fumes, vapors, or dusts.

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Short-read sequencers

DNA sequencers that produce short reads, often a few hundred base pairs in length.

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Biosafety cabinet

A type of safety cabinet that protects the user, the experiment, and the environment from potentially harmful substances.

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Glove box

A specialized enclosure that provides a sterile and controlled environment for working with hazardous materials.

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Recirculating fume hood

A type of fume hood that relies on filtration to make the air safe before releasing it back into the room.

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Genotyping

The process of determining the sequence of DNA bases in a specific region of DNA.

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Ducted fume hood

A type of fume hood that vents the contaminated air to the outside.

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Genome sequencing

The process of determining the complete DNA sequence of an organism.

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DNA fragment length

The length of a DNA fragment, often measured in base pairs.

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Fume hood sash

A sliding window at the front of a fume hood that allows access to the work area while keeping the user safe from fumes.

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High sash alarm

A protective feature of fume hoods that warns when the sash is raised too high, potentially reducing air velocity and safety.

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Single-pass air handling system

A type of ventilation system that uses fresh air only once before exhausting it.

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Return air system

A type of ventilation system that recirculates air within the laboratory, aiming to reduce energy consumption.

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Variable air volume (VAV) system

A system that adjusts the amount of air exhausted from a fume hood based on sash position, saving energy by reducing air flow when the sash is closed.

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Control panel

The component of a fume hood that houses essential controls like lights, exhaust fan, and alarms.

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Low airflow alarm

A safety feature in fume hoods that warns of low air flow, potentially indicating a malfunction in the ventilation system.

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Enclosed glovebox

A type of glove box designed for extremely hazardous materials, isolating the user from all direct contact with the work material and tools.

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Study Notes

Bioreactors

  • Bioreactors are manufactured devices supporting biologically active environments.
  • They can be vessels for chemical processes involving organisms or biochemically active substances.
  • Processes can be aerobic or anaerobic.
  • Bioreactors are typically cylindrical, ranging from liters to cubic meters.
  • They're commonly made of stainless steel.
  • Can also refer to devices for growing cells or tissues in cell culture (e.g., tissue engineering, biochemical/bioprocess engineering).
  • Classified as batch, fed-batch, or continuous (e.g., continuous stirred-tank reactor, chemostat) based on operation mode.
  • Bioreactors can support submerged cultures (organisms suspended in liquid) or immobilized cultures (organisms anchored to a solid medium).

Continuous Stirred-Tank Bioreactor

  • A continuous bioreactor type.
  • Organisms/substances are commonly in a liquid medium.
  • Organisms can be suspended or immobilized; immobilization requires no specialized attachment surfaces.
  • Continuous operation allows for larger scale operation than immobilized cultures.
  • Organisms are removed from the reactor continuously.

Bioreactor Design

  • A complex engineering task.
  • Environmental conditions (temperature, nutrient concentration, pH, dissolved gases, e.g., oxygen for aerobic fermentations) affect organism growth and productivity.
  • Maintaining optimal temperature may require cooling jackets, coils or external heat exchangers.
  • Nutrients may be added continuously (fed-batch) or charged at the start of fermentation.
  • pH is adjusted based on process needs.
  • Oxygen addition often required for aerobic fermentations; oxygen is difficult to add due to low solubility in water, leading to pressurized systems (e.g., for increasing solubility).
  • Cleaning is important to prevent fouling.

Photobioreactors (PBRs)

  • Incorporate light sources (natural or artificial).
  • Used for growing phototrophic microorganisms (e.g., cyanobacteria, algae, moss plants).
  • Photosynthesis is the primary energy source.
  • Generally, less risk of contamination from heterotrophic organisms compared to other bioreactors.

Sewage Treatment

  • Bioreactors are vital for purifying sewage.
  • In some systems, chemically inert mediums provide substrates for biological film growth.
  • Excess biological film is separated, usually in settling tanks.

Biosafety Cabinets (BSCs)

  • Enclosed, ventilated laboratory workspaces for safe handling of materials, especially if contaminated with pathogens.
  • Different types, varying in biocontainment.
  • Primarily protect the user and environment by HEPA-filtering exhaust air, removing harmful bacteria and viruses.
  • Secondary functions may include preventing spills, explosion protection.

DNA Sequencers

  • Scientific instruments for automating DNA sequencing.
  • Determine the order of DNA bases (G, C, A, T).
  • Often utilize optical instruments (detecting signals from fluorochromes attached to nucleotides).
  • The Sanger sequencing method, which formed the basis of the first generation of DNA sequencers, was used in the human genome project.

Fume Hoods

  • Local ventilation devices limiting exposure to hazardous gases, vapors, and dusts.
  • Two main types: ducted (exhausts gas outside the facility) and recirculating (filters air).
  • Contain a sash window to control air flow.
  • Control panels regulate various functions including internal lighting, air flow warnings, and fan control.

Standard Glove Boxes

  • Used for hazardous materials, isolating the user, maintaining negative pressure (or controlled pressure) to prevent leaks.

Cleanrooms

  • Engineered spaces with low concentrations of airborne particulates, for e.g. semiconductors, scientific research.
  • Controlled humidity and temperature.
  • Filtered air using HEPA and ULPA filters.
  • Carefully chosen materials to minimize airborne particle generation.

Personnel Contamination of Cleanrooms

  • Users are the primary source of contamination in cleanroom environments
  • Controlling microbial contamination is crucial.

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