Cell Differentiation and Tissue Culture (Code: MB 303)

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What is a common characteristic of bacterial contamination in cell culture?

Bacterial colonies are genetically identical

Detection usually involves visible turbidity/cloudiness (correct)

Bacterial contamination always leads to a rise in pH

Bacterial cells are extremely large in size

What sets mycoplasma contamination apart from other types of contamination?

Mycoplasma usually go unnoticed until reaching high densities (correct)

Mycoplasma bacteria have a cell wall

Mycoplasma contamination always results in visible mold growth

Mycoplasma are relatively easy to detect due to their large size

What is the distinguishing feature of yeast contamination in cell culture?

Yeast cells are unicellular (correct)

Yeast cells are multicellular in nature

Yeast contamination leads to a drop in pH

Yeast cells commonly form colonies with genetically distinct nuclei

Which type of contamination grows as multicellular filaments called hyphae?

Mold contamination Signup and view all the answers

How do infected cell cultures with bacterial contamination usually appear?

Turbid or cloudy Signup and view all the answers

Why are mycoplasma difficult to detect until they reach high densities?

Mycoplasma lack a cell wall making them challenging to spot Signup and view all the answers

Which of the following is NOT a key element of aseptic technique?

Use of non-sterile supplies Signup and view all the answers

What is the primary purpose of wearing personal protective equipment during cell culture work?

To protect the operator from hazardous materials Signup and view all the answers

Which of the following is NOT recommended for routine cleaning of the work surface in a tissue culture laboratory?

Spraying with a bleach solution Signup and view all the answers

What is the primary reason for maintaining good personal hygiene in a tissue culture laboratory?

To reduce the probability of contamination from shed skin and clothing Signup and view all the answers

Which of the following statements about sterile reagents and media is correct?

They should be handled using aseptic techniques Signup and view all the answers

What is the primary function of disinfecting the work surface in a tissue culture laboratory?

To maintain a sterile environment for cell culture work Signup and view all the answers

What is the primary purpose of autoclaving and sterile filtration in cell culture procedures?

To remove any bacterial or fungal contaminants from reagents and media Signup and view all the answers

Which statement accurately describes the importance of sterile handling techniques in cell culture?

It minimizes the risk of introducing bacterial or fungal contaminants Signup and view all the answers

Which of the following statements about commercial reagents and media is accurate?

They undergo strict quality control to ensure sterility Signup and view all the answers

What is the primary purpose of using a biosafety cabinet during cell culture procedures?

To provide a sterile environment for handling cells and reagents Signup and view all the answers

Which of the following statements accurately describes the importance of sterilizing reagents prepared in the laboratory?

It ensures that the reagents are free from any biological contaminants Signup and view all the answers

Which of the following techniques is typically used for sterilizing heat-sensitive solutions or reagents in cell culture?

Sterile filtration Signup and view all the answers

Cell Differentiation and Tissue Culture (Code: MB 303) - Lecture 4

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