Podcast
Questions and Answers
What is a common characteristic of bacterial contamination in cell culture?
What is a common characteristic of bacterial contamination in cell culture?
- Bacterial colonies are genetically identical
- Detection usually involves visible turbidity/cloudiness (correct)
- Bacterial contamination always leads to a rise in pH
- Bacterial cells are extremely large in size
What sets mycoplasma contamination apart from other types of contamination?
What sets mycoplasma contamination apart from other types of contamination?
- Mycoplasma usually go unnoticed until reaching high densities (correct)
- Mycoplasma bacteria have a cell wall
- Mycoplasma contamination always results in visible mold growth
- Mycoplasma are relatively easy to detect due to their large size
What is the distinguishing feature of yeast contamination in cell culture?
What is the distinguishing feature of yeast contamination in cell culture?
- Yeast cells are unicellular (correct)
- Yeast cells are multicellular in nature
- Yeast contamination leads to a drop in pH
- Yeast cells commonly form colonies with genetically distinct nuclei
Which type of contamination grows as multicellular filaments called hyphae?
Which type of contamination grows as multicellular filaments called hyphae?
How do infected cell cultures with bacterial contamination usually appear?
How do infected cell cultures with bacterial contamination usually appear?
Why are mycoplasma difficult to detect until they reach high densities?
Why are mycoplasma difficult to detect until they reach high densities?
Which of the following is NOT a key element of aseptic technique?
Which of the following is NOT a key element of aseptic technique?
What is the primary purpose of wearing personal protective equipment during cell culture work?
What is the primary purpose of wearing personal protective equipment during cell culture work?
Which of the following is NOT recommended for routine cleaning of the work surface in a tissue culture laboratory?
Which of the following is NOT recommended for routine cleaning of the work surface in a tissue culture laboratory?
What is the primary reason for maintaining good personal hygiene in a tissue culture laboratory?
What is the primary reason for maintaining good personal hygiene in a tissue culture laboratory?
Which of the following statements about sterile reagents and media is correct?
Which of the following statements about sterile reagents and media is correct?
What is the primary function of disinfecting the work surface in a tissue culture laboratory?
What is the primary function of disinfecting the work surface in a tissue culture laboratory?
What is the primary purpose of autoclaving and sterile filtration in cell culture procedures?
What is the primary purpose of autoclaving and sterile filtration in cell culture procedures?
Which statement accurately describes the importance of sterile handling techniques in cell culture?
Which statement accurately describes the importance of sterile handling techniques in cell culture?
Which of the following statements about commercial reagents and media is accurate?
Which of the following statements about commercial reagents and media is accurate?
What is the primary purpose of using a biosafety cabinet during cell culture procedures?
What is the primary purpose of using a biosafety cabinet during cell culture procedures?
Which of the following statements accurately describes the importance of sterilizing reagents prepared in the laboratory?
Which of the following statements accurately describes the importance of sterilizing reagents prepared in the laboratory?
Which of the following techniques is typically used for sterilizing heat-sensitive solutions or reagents in cell culture?
Which of the following techniques is typically used for sterilizing heat-sensitive solutions or reagents in cell culture?
