18 Questions
What is a common characteristic of bacterial contamination in cell culture?
Detection usually involves visible turbidity/cloudiness
What sets mycoplasma contamination apart from other types of contamination?
Mycoplasma usually go unnoticed until reaching high densities
What is the distinguishing feature of yeast contamination in cell culture?
Yeast cells are unicellular
Which type of contamination grows as multicellular filaments called hyphae?
Mold contamination
How do infected cell cultures with bacterial contamination usually appear?
Turbid or cloudy
Why are mycoplasma difficult to detect until they reach high densities?
Mycoplasma lack a cell wall making them challenging to spot
Which of the following is NOT a key element of aseptic technique?
Use of non-sterile supplies
What is the primary purpose of wearing personal protective equipment during cell culture work?
To protect the operator from hazardous materials
Which of the following is NOT recommended for routine cleaning of the work surface in a tissue culture laboratory?
Spraying with a bleach solution
What is the primary reason for maintaining good personal hygiene in a tissue culture laboratory?
To reduce the probability of contamination from shed skin and clothing
Which of the following statements about sterile reagents and media is correct?
They should be handled using aseptic techniques
What is the primary function of disinfecting the work surface in a tissue culture laboratory?
To maintain a sterile environment for cell culture work
What is the primary purpose of autoclaving and sterile filtration in cell culture procedures?
To remove any bacterial or fungal contaminants from reagents and media
Which statement accurately describes the importance of sterile handling techniques in cell culture?
It minimizes the risk of introducing bacterial or fungal contaminants
Which of the following statements about commercial reagents and media is accurate?
They undergo strict quality control to ensure sterility
What is the primary purpose of using a biosafety cabinet during cell culture procedures?
To provide a sterile environment for handling cells and reagents
Which of the following statements accurately describes the importance of sterilizing reagents prepared in the laboratory?
It ensures that the reagents are free from any biological contaminants
Which of the following techniques is typically used for sterilizing heat-sensitive solutions or reagents in cell culture?
Sterile filtration
This quiz covers the requirements of a tissue culture laboratory and aseptic techniques essential for successful cell culture. Topics include the importance of keeping cells contamination-free and the use of barriers to prevent environmental microorganism exposure.
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