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Cell Cycle Regulation Quiz

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Questions and Answers

What method was used to analyze the proteins present in sea urchin eggs in the experiment mentioned?

Radioactive labeling (correct)

Western blotting

Immunofluorescence

PCR amplification

What was observed about the protein that gradually built up but was rapidly degraded when cells divided?

Its level peaked just before cell division (correct)

Its level increased continuously during cell division

It was not influenced by cell division

It was stable throughout the cell cycle

What is the term used for the proteins that fluctuated in levels as cells progressed through divisions?

Hormones

Cytokines

Cyclins (correct)

Enzymes

When does the level of cyclin fall according to the observations made in the experiment?

At the end of mitosis Signup and view all the answers

What was correlated with the peak of cyclin levels in the experiment?

Higher MPF activity Signup and view all the answers

How many subunits were revealed in the biochemical purification of MPF?

2 subunits Signup and view all the answers

What is the regulatory subunit that activates the cyclin dependent kinase?

Cyclin B Signup and view all the answers

Which of the following phosphorylation events on the cyclin dependent kinase is inhibitory?

Phosphorylation on Tyrosine 15 Signup and view all the answers

What is the role of the Cdc25 phosphatase in the cell cycle regulation?

Cdc25 activates the CDK-cyclin complex by removing inhibitory phosphorylations Signup and view all the answers

What is the function of the T-loop in the cyclin dependent kinase structure?

The T-loop blocks access to the active site of the CDK Signup and view all the answers

Which of the following is not a mechanism of regulation of the cyclin dependent kinase activity?

Protein degradation Signup and view all the answers

What is the relationship between the Cdc2 protein in fission yeast and the Cdc28 protein in budding yeast?

Cdc2 is the equivalent of Cdc28 in fission yeast Signup and view all the answers