Cell Cycle Regulation Quiz
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Questions and Answers

What method was used to analyze the proteins present in sea urchin eggs in the experiment mentioned?

  • Radioactive labeling (correct)
  • Western blotting
  • Immunofluorescence
  • PCR amplification
  • What was observed about the protein that gradually built up but was rapidly degraded when cells divided?

  • Its level peaked just before cell division (correct)
  • Its level increased continuously during cell division
  • It was not influenced by cell division
  • It was stable throughout the cell cycle
  • What is the term used for the proteins that fluctuated in levels as cells progressed through divisions?

  • Hormones
  • Cytokines
  • Cyclins (correct)
  • Enzymes
  • When does the level of cyclin fall according to the observations made in the experiment?

    <p>At the end of mitosis</p> Signup and view all the answers

    What was correlated with the peak of cyclin levels in the experiment?

    <p>Higher MPF activity</p> Signup and view all the answers

    How many subunits were revealed in the biochemical purification of MPF?

    <p>2 subunits</p> Signup and view all the answers

    What is the regulatory subunit that activates the cyclin dependent kinase?

    <p>Cyclin B</p> Signup and view all the answers

    Which of the following phosphorylation events on the cyclin dependent kinase is inhibitory?

    <p>Phosphorylation on Tyrosine 15</p> Signup and view all the answers

    What is the role of the Cdc25 phosphatase in the cell cycle regulation?

    <p>Cdc25 activates the CDK-cyclin complex by removing inhibitory phosphorylations</p> Signup and view all the answers

    What is the function of the T-loop in the cyclin dependent kinase structure?

    <p>The T-loop blocks access to the active site of the CDK</p> Signup and view all the answers

    Which of the following is not a mechanism of regulation of the cyclin dependent kinase activity?

    <p>Protein degradation</p> Signup and view all the answers

    What is the relationship between the Cdc2 protein in fission yeast and the Cdc28 protein in budding yeast?

    <p>Cdc2 is the equivalent of Cdc28 in fission yeast</p> Signup and view all the answers

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