Biotech 8-Cell Cycle Regulation Part B
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Questions and Answers

Which of the following is NOT a key regulatory protein involved in cell cycle progression?

  • p53 (correct)
  • Rb
  • Cdk1
  • Cyclin B
  • Which cyclin-CDK complex operates at the G1/S transition?

  • Cyclin B/Cdk1
  • Cyclin E/Cdk2
  • Cyclin A/Cdk1
  • Cyclin D/Cdk4/6 (correct)
  • Which of the following is a CKI (cyclin-dependent kinase inhibitor)?

  • Cdk1
  • Cyclin B
  • Rb
  • p21 (correct)
  • Which proteins are phosphorylated in the active form of MPF (maturation-promoting factor)?

    <p>Cdc2 and Cyclin B</p> Signup and view all the answers

    What is the role of CDC25 in cell cycle regulation?

    <p>Promotes M phase</p> Signup and view all the answers

    How is MPF regulated?

    <p>All of the above</p> Signup and view all the answers

    Which of the following is true about the Rb protein?

    <p>It is a transcription factor</p> Signup and view all the answers

    Which of the following is true about p53?

    <p>It is a transcription factor</p> Signup and view all the answers

    Which CDK4/6 inhibitor is marketed by Pfizer?

    <p>Ibrance</p> Signup and view all the answers

    CDK4/6 inhibitors are usually administered in combination with hormone therapies to treat which type of breast cancer?

    <p>HR+ and HER2-</p> Signup and view all the answers

    What is the main function of MPF at M phase?

    <p>Regulation of MPF</p> Signup and view all the answers

    Which of the following is NOT a component of MPF?

    <p>Cyclin D</p> Signup and view all the answers

    What is the role of CKIs in the cell cycle?

    <p>Regulation of Cyclins and CDKs</p> Signup and view all the answers

    Which of the following is NOT involved in the regulation of Cyclin D / CDK4//6?

    <p>Drugs that target CDK4/6</p> Signup and view all the answers

    What is the molecular basis of cell cycle regulation?

    <p>Studies in mammalian cells</p> Signup and view all the answers

    Which of the following is NOT a method used to study the history of the discovery of MPF?

    <p>Studies in yeast</p> Signup and view all the answers

    What are the substrates of Cdc2?

    <p>Cyclin B</p> Signup and view all the answers

    Which of the following is NOT a topic covered in the lectures on Biochemical foundations of pharmaceutical biotechnology?

    <p>Control of cell behavior A: Cell membrane and transport, drugs that impact cell transport</p> Signup and view all the answers

    Which cell types were used in the studies to understand the molecular mechanism of cell cycle regulation?

    <p>Frog (Xenopus) oocytes</p> Signup and view all the answers

    What is the name of the factor found in the cytoplasm of M phase cells that can cause G2 arrested cells to enter M phase?

    <p>MPF (Maturation Promoting Factor)</p> Signup and view all the answers

    Which genes were found to be important for progression from G1 to S in yeast?

    <p>Cdc28 (gene in Saccharomyces cerevisiae) and Cdc2 (gene in Saccharomyces pombe)</p> Signup and view all the answers

    What are the proteins encoded by certain genes that are required for yeast to pass through various phases of the cell cycle called?

    <p>cdcs</p> Signup and view all the answers

    What is the name of the proteins in sea urchin eggs that show a cyclic pattern throughout the cell cycle?

    <p>Cyclins</p> Signup and view all the answers

    What is the name of the phosphatase that dephosphorylates two of Cdc2's phospho-sites, activating MPF?

    <p>A phosphatase</p> Signup and view all the answers

    What is the role of Cdc2 (CDK1) in the cell cycle?

    <p>Phosphorylates substrates important for M phase</p> Signup and view all the answers

    What happens when Cdc2 phosphorylates Cyclin B?

    <p>Cyclin B gets degraded</p> Signup and view all the answers

    What are the substrates of Cdc2 (CDK1) in the cell cycle?

    <p>Nuclear lamins, Histone H1, MAPs</p> Signup and view all the answers

    What are the two components of MPF from Xenopus?

    <p>A 34 kd protein and a 45 kd protein</p> Signup and view all the answers

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