Cell Cycle Cloning

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30 Questions

Which phase of the cell cycle involves the division of genetic information?

M phase

What is the purpose of the G1/S checkpoint?

To ensure DNA replication is complete

Which phase of the cell cycle is responsible for DNA synthesis?

S phase

What is the purpose of the G2/M checkpoint?

To prepare for cell division

Which phase of the cell cycle is known as the growth phase?

G1 phase

Which phase of the cell cycle is responsible for the synthesis of nuclear DNA?

S phase

What is the purpose of the G0 phase in the cell cycle?

Resting state for cells in G1

During which phase of mitosis do sister chromatids separate and migrate to opposite poles of the spindle?

Anaphase

What is the purpose of cytokinesis in the cell cycle?

Formation of two separate daughter cells

What are the genetic consequences of the cell cycle?

Two genetically identical cells

Which enzyme removes inhibitory phosphorylations from CDK1 during the cell cycle?

Cdc25c phosphatase

What is the process of programmed cell death called?

Apoptosis

Which molecule becomes exposed on the surface of apoptotic cells?

Phosphatidylserine

What is the purpose of macrophages and dendritic cells in apoptosis?

To degrade apoptotic cells

What is the goal of recombinant DNA technology?

To combine DNA from different sources

Which technique is used to determine patterns of mRNA production and can analyze thousands of genes at the same time?

Microarray

Which technique involves the conversion of mRNA to cDNA and the use of a fluorescent tag to determine the amount of mRNA in a sample?

Microarray

Which technique involves the use of a radiolabeled probe specific for target mRNA to analyze the amount and size of particular mRNA in a sample?

Northern Blots

Which technique involves the use of an enzyme-linked antibody to detect the amount of protein in a sample?

ELISA

Which technique involves the separation of proteins by electrophoresis and the use of a labeled antibody as a probe?

Western Blots

Which of the following is NOT a property of a cloning vector?

It is a small, circular, extrachromosomal DNA molecule

What is the purpose of transformation in DNA cloning?

To introduce foreign DNA into a bacterial or yeast cell

What is the difference between a genomic DNA library and a cDNA library?

Genomic DNA library contains all DNA sequences transcribed into mRNA, while cDNA library contains only a subset of DNA sequences

What is the purpose of the Sanger dideoxy method in DNA sequencing?

To determine the base sequence of cloned DNA fragments

What is the purpose of southern blotting in DNA analysis?

To detect mutations in DNA

Which of the following is true about cyclins?

Cyclins regulate specific phases of the cell cycle

Which of the following is true about cyclin-dependent kinases (CDKs)?

CDKs are regulated by cyclin levels

Which of the following is true about checkpoint regulation in the cell cycle?

Checkpoints delay progression to the next phase/step

Which of the following is true about tumor suppressors and checkpoints?

Retinoblastoma (RB) protein prevents entry into S phase

Which of the following is true about p53?

p53 stimulates the transcription of cyclin-dependent kinase inhibitors (CKI)

Test your knowledge on cell cycle regulation and cell death mechanisms in this quiz. Learn about the role of CDK1 and cyclin B in cell cycle progression, as well as the functions of Cdc25c phosphatase. Explore the differences between necrosis and apoptosis in cell death processes.

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