Cell Cycle Cloning
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Cell Cycle Cloning

Test your knowledge on cell cycle regulation and cell death mechanisms in this quiz. Learn about the role of CDK1 and cyclin B in cell cycle progression, as well as the functions of Cdc25c phosphatase. Explore the differences between necrosis and apoptosis in cell death processes.

Created by
@EffectualJubilation

Questions and Answers

Which phase of the cell cycle involves the division of genetic information?

M phase

What is the purpose of the G1/S checkpoint?

To ensure DNA replication is complete

Which phase of the cell cycle is responsible for DNA synthesis?

S phase

What is the purpose of the G2/M checkpoint?

<p>To prepare for cell division</p> Signup and view all the answers

Which phase of the cell cycle is known as the growth phase?

<p>G1 phase</p> Signup and view all the answers

Which phase of the cell cycle is responsible for the synthesis of nuclear DNA?

<p>S phase</p> Signup and view all the answers

What is the purpose of the G0 phase in the cell cycle?

<p>Resting state for cells in G1</p> Signup and view all the answers

During which phase of mitosis do sister chromatids separate and migrate to opposite poles of the spindle?

<p>Anaphase</p> Signup and view all the answers

What is the purpose of cytokinesis in the cell cycle?

<p>Formation of two separate daughter cells</p> Signup and view all the answers

What are the genetic consequences of the cell cycle?

<p>Two genetically identical cells</p> Signup and view all the answers

Which enzyme removes inhibitory phosphorylations from CDK1 during the cell cycle?

<p>Cdc25c phosphatase</p> Signup and view all the answers

What is the process of programmed cell death called?

<p>Apoptosis</p> Signup and view all the answers

Which molecule becomes exposed on the surface of apoptotic cells?

<p>Phosphatidylserine</p> Signup and view all the answers

What is the purpose of macrophages and dendritic cells in apoptosis?

<p>To degrade apoptotic cells</p> Signup and view all the answers

What is the goal of recombinant DNA technology?

<p>To combine DNA from different sources</p> Signup and view all the answers

Which technique is used to determine patterns of mRNA production and can analyze thousands of genes at the same time?

<p>Microarray</p> Signup and view all the answers

Which technique involves the conversion of mRNA to cDNA and the use of a fluorescent tag to determine the amount of mRNA in a sample?

<p>Microarray</p> Signup and view all the answers

Which technique involves the use of a radiolabeled probe specific for target mRNA to analyze the amount and size of particular mRNA in a sample?

<p>Northern Blots</p> Signup and view all the answers

Which technique involves the use of an enzyme-linked antibody to detect the amount of protein in a sample?

<p>ELISA</p> Signup and view all the answers

Which technique involves the separation of proteins by electrophoresis and the use of a labeled antibody as a probe?

<p>Western Blots</p> Signup and view all the answers

Which of the following is NOT a property of a cloning vector?

<p>It is a small, circular, extrachromosomal DNA molecule</p> Signup and view all the answers

What is the purpose of transformation in DNA cloning?

<p>To introduce foreign DNA into a bacterial or yeast cell</p> Signup and view all the answers

What is the difference between a genomic DNA library and a cDNA library?

<p>Genomic DNA library contains all DNA sequences transcribed into mRNA, while cDNA library contains only a subset of DNA sequences</p> Signup and view all the answers

What is the purpose of the Sanger dideoxy method in DNA sequencing?

<p>To determine the base sequence of cloned DNA fragments</p> Signup and view all the answers

What is the purpose of southern blotting in DNA analysis?

<p>To detect mutations in DNA</p> Signup and view all the answers

Which of the following is true about cyclins?

<p>Cyclins regulate specific phases of the cell cycle</p> Signup and view all the answers

Which of the following is true about cyclin-dependent kinases (CDKs)?

<p>CDKs are regulated by cyclin levels</p> Signup and view all the answers

Which of the following is true about checkpoint regulation in the cell cycle?

<p>Checkpoints delay progression to the next phase/step</p> Signup and view all the answers

Which of the following is true about tumor suppressors and checkpoints?

<p>Retinoblastoma (RB) protein prevents entry into S phase</p> Signup and view all the answers

Which of the following is true about p53?

<p>p53 stimulates the transcription of cyclin-dependent kinase inhibitors (CKI)</p> Signup and view all the answers

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