Cell Cycle Cloning
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Questions and Answers

Which phase of the cell cycle involves the division of genetic information?

  • M phase (correct)
  • G1 phase
  • S phase
  • G2 phase

    • What is the purpose of the G1/S checkpoint?

  • To monitor DNA damage
  • To ensure DNA replication is complete (correct)
  • To prepare for cell division
  • To regulate cell growth

    • Which phase of the cell cycle is responsible for DNA synthesis?

  • G2 phase
  • M phase
  • S phase (correct)
  • G1 phase

    • What is the purpose of the G2/M checkpoint?

    <p>To prepare for cell division</p> Signup and view all the answers

    Which phase of the cell cycle is known as the growth phase?

    <p>G1 phase</p> Signup and view all the answers

    Which phase of the cell cycle is responsible for the synthesis of nuclear DNA?

    <p>S phase</p> Signup and view all the answers

    What is the purpose of the G0 phase in the cell cycle?

    <p>Resting state for cells in G1</p> Signup and view all the answers

    During which phase of mitosis do sister chromatids separate and migrate to opposite poles of the spindle?

    <p>Anaphase</p> Signup and view all the answers

    What is the purpose of cytokinesis in the cell cycle?

    <p>Formation of two separate daughter cells</p> Signup and view all the answers

    What are the genetic consequences of the cell cycle?

    <p>Two genetically identical cells</p> Signup and view all the answers

    Which enzyme removes inhibitory phosphorylations from CDK1 during the cell cycle?

    <p>Cdc25c phosphatase</p> Signup and view all the answers

    What is the process of programmed cell death called?

    <p>Apoptosis</p> Signup and view all the answers

    Which molecule becomes exposed on the surface of apoptotic cells?

    <p>Phosphatidylserine</p> Signup and view all the answers

    What is the purpose of macrophages and dendritic cells in apoptosis?

    <p>To degrade apoptotic cells</p> Signup and view all the answers

    What is the goal of recombinant DNA technology?

    <p>To combine DNA from different sources</p> Signup and view all the answers

    Which technique is used to determine patterns of mRNA production and can analyze thousands of genes at the same time?

    <p>Microarray</p> Signup and view all the answers

    Which technique involves the conversion of mRNA to cDNA and the use of a fluorescent tag to determine the amount of mRNA in a sample?

    <p>Microarray</p> Signup and view all the answers

    Which technique involves the use of a radiolabeled probe specific for target mRNA to analyze the amount and size of particular mRNA in a sample?

    <p>Northern Blots</p> Signup and view all the answers

    Which technique involves the use of an enzyme-linked antibody to detect the amount of protein in a sample?

    <p>ELISA</p> Signup and view all the answers

    Which technique involves the separation of proteins by electrophoresis and the use of a labeled antibody as a probe?

    <p>Western Blots</p> Signup and view all the answers

    Which of the following is NOT a property of a cloning vector?

    <p>It is a small, circular, extrachromosomal DNA molecule</p> Signup and view all the answers

    What is the purpose of transformation in DNA cloning?

    <p>To introduce foreign DNA into a bacterial or yeast cell</p> Signup and view all the answers

    What is the difference between a genomic DNA library and a cDNA library?

    <p>Genomic DNA library contains all DNA sequences transcribed into mRNA, while cDNA library contains only a subset of DNA sequences</p> Signup and view all the answers

    What is the purpose of the Sanger dideoxy method in DNA sequencing?

    <p>To determine the base sequence of cloned DNA fragments</p> Signup and view all the answers

    What is the purpose of southern blotting in DNA analysis?

    <p>To detect mutations in DNA</p> Signup and view all the answers

    Which of the following is true about cyclins?

    <p>Cyclins regulate specific phases of the cell cycle</p> Signup and view all the answers

    Which of the following is true about cyclin-dependent kinases (CDKs)?

    <p>CDKs are regulated by cyclin levels</p> Signup and view all the answers

    Which of the following is true about checkpoint regulation in the cell cycle?

    <p>Checkpoints delay progression to the next phase/step</p> Signup and view all the answers

    Which of the following is true about tumor suppressors and checkpoints?

    <p>Retinoblastoma (RB) protein prevents entry into S phase</p> Signup and view all the answers

    Which of the following is true about p53?

    <p>p53 stimulates the transcription of cyclin-dependent kinase inhibitors (CKI)</p> Signup and view all the answers

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