Podcast
Questions and Answers
What triggers the movement from one phase of the cell cycle to another?
What triggers the movement from one phase of the cell cycle to another?
- Centrosomes
- Cyclins (correct)
- Cyclosomes
- Cytokines
Which phase of interphase is primarily focused on DNA replication?
Which phase of interphase is primarily focused on DNA replication?
- M phase
- G2 phase
- G1 phase
- S phase (correct)
What occurs during the G1 phase of interphase?
What occurs during the G1 phase of interphase?
- Cell growth and synthesis of proteins (correct)
- DNA synthesis
- Chromosome separation
- Cell division
What is the main role of the G2 phase in the cell cycle?
What is the main role of the G2 phase in the cell cycle?
What happens to the cell during the M phase?
What happens to the cell during the M phase?
What is the primary function of cytokinesis?
What is the primary function of cytokinesis?
Which type of cell division results in genetically identical daughter cells?
Which type of cell division results in genetically identical daughter cells?
How often do onion root tip cells typically divide during the cell cycle?
How often do onion root tip cells typically divide during the cell cycle?
What is the primary function of xylem vessels?
What is the primary function of xylem vessels?
Which of the following statements accurately describes phloem vessel cells?
Which of the following statements accurately describes phloem vessel cells?
What is the significance of the thickening of outer walls in xylem cells?
What is the significance of the thickening of outer walls in xylem cells?
What is a key adaptation of skeletal muscle cells?
What is a key adaptation of skeletal muscle cells?
How does ciliated epithelium aid in protecting the body from infections?
How does ciliated epithelium aid in protecting the body from infections?
What is the primary function of squamous epithelium?
What is the primary function of squamous epithelium?
Which characteristic of cartilage contributes to its flexibility?
Which characteristic of cartilage contributes to its flexibility?
What is meant by potency in the context of stem cells?
What is meant by potency in the context of stem cells?
What distinguishes totipotent stem cells from other types of stem cells?
What distinguishes totipotent stem cells from other types of stem cells?
Which of the following is NOT a function of muscle cells?
Which of the following is NOT a function of muscle cells?
What is the end result of cytokinesis in meiosis I?
What is the end result of cytokinesis in meiosis I?
Which process results in genetic variation due to the exchange of alleles?
Which process results in genetic variation due to the exchange of alleles?
In meiosis II, what happens during Anaphase II?
In meiosis II, what happens during Anaphase II?
What occurs during Metaphase I of meiosis?
What occurs during Metaphase I of meiosis?
How does independent assortment increase genetic diversity?
How does independent assortment increase genetic diversity?
How many combinations of chromosomes can be produced in humans due to independent assortment alone?
How many combinations of chromosomes can be produced in humans due to independent assortment alone?
What is the primary structural feature that allows erythrocytes to function effectively?
What is the primary structural feature that allows erythrocytes to function effectively?
What phase precedes Prophase II in meiosis?
What phase precedes Prophase II in meiosis?
What phase of meiosis does crossing over occur during?
What phase of meiosis does crossing over occur during?
What is true about the DNA content during meiosis II?
What is true about the DNA content during meiosis II?
What are chiasmata?
What are chiasmata?
Which stage in meiosis I leads to two haploid daughter cells?
Which stage in meiosis I leads to two haploid daughter cells?
How does meiosis contribute to natural selection?
How does meiosis contribute to natural selection?
Which option describes the result of gamete fusion during fertilization?
Which option describes the result of gamete fusion during fertilization?
What type of stem cells can differentiate into any cell type found in an embryo?
What type of stem cells can differentiate into any cell type found in an embryo?
Which of the following best describes multipotent stem cells?
Which of the following best describes multipotent stem cells?
Which cell type is specifically produced from multipotent stem cells in the bone marrow?
Which cell type is specifically produced from multipotent stem cells in the bone marrow?
During erythropoiesis, the ejection of the nucleus from the precursor cell allows for what adaptation?
During erythropoiesis, the ejection of the nucleus from the precursor cell allows for what adaptation?
Which statement accurately describes how neutrophils are structurally modified during differentiation?
Which statement accurately describes how neutrophils are structurally modified during differentiation?
What is the main function of erythrocytes in the body?
What is the main function of erythrocytes in the body?
Where in the body can multipotent adult stem cells be found?
Where in the body can multipotent adult stem cells be found?
Which of the following is NOT a characteristic of totipotent stem cells?
Which of the following is NOT a characteristic of totipotent stem cells?
What clinical application is being researched with adult stem cells?
What clinical application is being researched with adult stem cells?
The biconcave shape of erythrocytes enhances their ability to function by providing what advantage?
The biconcave shape of erythrocytes enhances their ability to function by providing what advantage?
During which phase of the cell cycle does the most mRNA production occur?
During which phase of the cell cycle does the most mRNA production occur?
What is the primary purpose of checkpoints in the cell cycle?
What is the primary purpose of checkpoints in the cell cycle?
What occurs during prophase of mitosis?
What occurs during prophase of mitosis?
Which phase is characterized by sister chromatids being pulled to opposite poles?
Which phase is characterized by sister chromatids being pulled to opposite poles?
How many checkpoints are present in the cell cycle?
How many checkpoints are present in the cell cycle?
What specific event happens during the S phase of the cell cycle?
What specific event happens during the S phase of the cell cycle?
Which structure is essential for the attachment of spindle fibres to chromosomes during metaphase?
Which structure is essential for the attachment of spindle fibres to chromosomes during metaphase?
What happens to the sister chromatids during metaphase?
What happens to the sister chromatids during metaphase?
What is a key characteristic of telophase in mitosis?
What is a key characteristic of telophase in mitosis?
What does the diploid number represent in human cells?
What does the diploid number represent in human cells?
What type of regions in plants are primarily responsible for growth?
What type of regions in plants are primarily responsible for growth?
Why might a cell undergo self-destruction during the cell cycle?
Why might a cell undergo self-destruction during the cell cycle?
How is mitosis in animal cells simplified in educational diagrams?
How is mitosis in animal cells simplified in educational diagrams?
What is the immediate outcome of mitosis?
What is the immediate outcome of mitosis?
What is the primary function of red blood cells?
What is the primary function of red blood cells?
What adaptation do sperm cells have that aids their motility?
What adaptation do sperm cells have that aids their motility?
What is a primary function of mitosis in multicellular organisms?
What is a primary function of mitosis in multicellular organisms?
How do root hair cells maximize their ability to absorb water?
How do root hair cells maximize their ability to absorb water?
What unique structure do neutrophils utilize to engulf microorganisms?
What unique structure do neutrophils utilize to engulf microorganisms?
Which stage of mitosis is characterized by chromosomes aligning at the center of the cell?
Which stage of mitosis is characterized by chromosomes aligning at the center of the cell?
Which cell type has adaptations to easily diffuse gases?
Which cell type has adaptations to easily diffuse gases?
What occurs during Prophase I of meiosis that is not seen in mitosis?
What occurs during Prophase I of meiosis that is not seen in mitosis?
How do optical microscopes compare to other types of microscopes?
How do optical microscopes compare to other types of microscopes?
Why do palisade cells have a large number of chloroplasts?
Why do palisade cells have a large number of chloroplasts?
What is the function of goblet cells in ciliated epithelium?
What is the function of goblet cells in ciliated epithelium?
In which phase of meiosis do homologous chromosomes separate?
In which phase of meiosis do homologous chromosomes separate?
How do guard cells regulate the opening of stomata?
How do guard cells regulate the opening of stomata?
What is significant about the daughter cells produced at the end of mitosis?
What is significant about the daughter cells produced at the end of mitosis?
What type of tissue is formed when similar specialized cells group together?
What type of tissue is formed when similar specialized cells group together?
What process follows telophase in both mitosis and meiosis?
What process follows telophase in both mitosis and meiosis?
What is the major function of ciliated epithelium in the respiratory system?
What is the major function of ciliated epithelium in the respiratory system?
Which of the following best describes asexual reproduction?
Which of the following best describes asexual reproduction?
What is a common limitation when identifying the stage of mitosis in cells?
What is a common limitation when identifying the stage of mitosis in cells?
What characteristic of red blood cells allows for maximum oxygen-carrying capacity?
What characteristic of red blood cells allows for maximum oxygen-carrying capacity?
What is the role of mitochondria in root hair cells?
What is the role of mitochondria in root hair cells?
What occurs during Cytokinesis in plant cells?
What occurs during Cytokinesis in plant cells?
What adaptation allows squamous epithelial cells to effectively cover and protect organs?
What adaptation allows squamous epithelial cells to effectively cover and protect organs?
What does the term 'bivalent' refer to during Prophase I of meiosis?
What does the term 'bivalent' refer to during Prophase I of meiosis?
Which statement regarding the replacement of cells in humans is true?
Which statement regarding the replacement of cells in humans is true?
What function does the acrosome in sperm cells serve?
What function does the acrosome in sperm cells serve?
What role does crossing over play in meiosis?
What role does crossing over play in meiosis?
What might cause inconsistency in cell size or structure observed in specimen slides?
What might cause inconsistency in cell size or structure observed in specimen slides?
What process do neutrophils use to destroy pathogens?
What process do neutrophils use to destroy pathogens?
What is the role of the cambium in plants?
What is the role of the cambium in plants?
Which type of stem cells can develop into a limited range of cell types and are found in adult tissues?
Which type of stem cells can develop into a limited range of cell types and are found in adult tissues?
How do cambium cells differentiate to form xylem?
How do cambium cells differentiate to form xylem?
What is the primary function of lysosomes in neutrophils?
What is the primary function of lysosomes in neutrophils?
What type of stem cells are used in research and can differentiate into any cell type?
What type of stem cells are used in research and can differentiate into any cell type?
What causes the differentiation of cambium cells into xylem or phloem?
What causes the differentiation of cambium cells into xylem or phloem?
What characterizes adult stem cells compared to embryonic stem cells?
What characterizes adult stem cells compared to embryonic stem cells?
Which organelle is primarily involved in modifying proteins and lipids?
Which organelle is primarily involved in modifying proteins and lipids?
What happens to embryonic stem cells on day 5 after fertilization?
What happens to embryonic stem cells on day 5 after fertilization?
Why is the use of adult stem cells considered less controversial than embryonic stem cells?
Why is the use of adult stem cells considered less controversial than embryonic stem cells?
What is a key role of the nucleus in eukaryotic cells?
What is a key role of the nucleus in eukaryotic cells?
Which component of the Golgi apparatus is responsible for packaging proteins?
Which component of the Golgi apparatus is responsible for packaging proteins?
What is the primary function of mitochondria in cells?
What is the primary function of mitochondria in cells?
Which structure is responsible for the engulfing and destruction of old organelles?
Which structure is responsible for the engulfing and destruction of old organelles?
What unique feature do chloroplasts possess that is crucial for photosynthesis?
What unique feature do chloroplasts possess that is crucial for photosynthesis?
Which statement best describes the function of the plasma membrane?
Which statement best describes the function of the plasma membrane?
What role do centrioles play during cell division?
What role do centrioles play during cell division?
What are pili used for in prokaryotic cells?
What are pili used for in prokaryotic cells?
Which of the following organelles is involved in protein modification and processing?
Which of the following organelles is involved in protein modification and processing?
What is the main component of a plant cell wall?
What is the main component of a plant cell wall?
Which characteristic is true for both prokaryotic and eukaryotic cells?
Which characteristic is true for both prokaryotic and eukaryotic cells?
What is a common function of vacuoles in plant cells?
What is a common function of vacuoles in plant cells?
What is the main structural difference between eukaryotic and prokaryotic flagella?
What is the main structural difference between eukaryotic and prokaryotic flagella?
Which type of microscope allows you to visualize living cells in real time?
Which type of microscope allows you to visualize living cells in real time?
What are mesosomes theorized to be involved in?
What are mesosomes theorized to be involved in?
What is the main disadvantage of using a transmission electron microscope (TEM)?
What is the main disadvantage of using a transmission electron microscope (TEM)?
Which type of microscope uses a laser beam to produce clear, 3D images?
Which type of microscope uses a laser beam to produce clear, 3D images?
What is the maximum magnification achieved by a scanning electron microscope (SEM)?
What is the maximum magnification achieved by a scanning electron microscope (SEM)?
What does resolution in microscopy refer to?
What does resolution in microscopy refer to?
Which of the following methods is used to create contrast in a specimen before viewing it under a light microscope?
Which of the following methods is used to create contrast in a specimen before viewing it under a light microscope?
What type of images can a scanning electron microscope (SEM) produce that a transmission electron microscope (TEM) cannot?
What type of images can a scanning electron microscope (SEM) produce that a transmission electron microscope (TEM) cannot?
How is the magnification of a specimen calculated?
How is the magnification of a specimen calculated?
Which of the following is NOT a feature of electron microscopes?
Which of the following is NOT a feature of electron microscopes?
What is the maximum resolution achieved by a light microscope?
What is the maximum resolution achieved by a light microscope?
Which step is first when calibrating the eyepiece graticule?
Which step is first when calibrating the eyepiece graticule?
Flashcards
Cell cycle
Cell cycle
The regulated sequence of events that occurs between one cell division and the next; a continuous process.
Interphase
Interphase
The phase of the cell cycle where the cell grows & carries out its normal functions.
Mitosis or Nuclear Division
Mitosis or Nuclear Division
The phase of the cell cycle where the cell's nucleus divides.
Cytokinesis or Cell division
Cytokinesis or Cell division
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G1 Phase
G1 Phase
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S Phase
S Phase
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G2 Phase
G2 Phase
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Cyclins
Cyclins
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Acetic orcein
Acetic orcein
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Squashing technique
Squashing technique
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Mitosis
Mitosis
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Prophase
Prophase
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Metaphase
Metaphase
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Anaphase
Anaphase
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Telophase
Telophase
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Cytokinesis
Cytokinesis
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Asexual Reproduction
Asexual Reproduction
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Meiosis
Meiosis
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Meiosis I
Meiosis I
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Meiosis II
Meiosis II
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Prophase I
Prophase I
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Metaphase I
Metaphase I
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Anaphase I
Anaphase I
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Crossing Over
Crossing Over
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Independent Assortment
Independent Assortment
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Random Fusion of Gametes
Random Fusion of Gametes
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Telophase II and Cytokinesis
Telophase II and Cytokinesis
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Specialized Cells
Specialized Cells
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Structural Adaptations
Structural Adaptations
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Ribosomes
Ribosomes
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Erythrocytes
Erythrocytes
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Significance of Meiosis
Significance of Meiosis
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Totipotent
Totipotent
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Pluripotent
Pluripotent
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Multipotent
Multipotent
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Hematopoiesis
Hematopoiesis
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Erythropoiesis
Erythropoiesis
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Neutrophils
Neutrophils
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Stem Cell Therapy
Stem Cell Therapy
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Cell Specialization
Cell Specialization
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S Phase Checkpoint
S Phase Checkpoint
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G1 Checkpoint
G1 Checkpoint
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G2 Checkpoint
G2 Checkpoint
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Meristem
Meristem
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Squash Technique
Squash Technique
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Spindle Fibers
Spindle Fibers
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Centromere
Centromere
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Centrosome
Centrosome
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Kinetochores
Kinetochores
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Spindle Fibers
Spindle Fibers
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Chromosomes
Chromosomes
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Centromere
Centromere
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Metaphase Checkpoint
Metaphase Checkpoint
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DNA Replication
DNA Replication
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Cell Division
Cell Division
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Apoptosis
Apoptosis
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Xylem Vessel Cell
Xylem Vessel Cell
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Phloem Vessel Cell
Phloem Vessel Cell
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Muscle Cell
Muscle Cell
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Ciliated Epithelial Cell
Ciliated Epithelial Cell
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Squamous Epithelial Cell
Squamous Epithelial Cell
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Cartilage
Cartilage
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Stem Cell
Stem Cell
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Potency
Potency
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Totipotent Stem Cell
Totipotent Stem Cell
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Pluripotent Stem Cell
Pluripotent Stem Cell
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What is the function of red blood cells?
What is the function of red blood cells?
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What shape are red blood cells and why?
What shape are red blood cells and why?
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Why are red blood cells anucleated?
Why are red blood cells anucleated?
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How do red blood cells navigate tight spaces?
How do red blood cells navigate tight spaces?
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What is the function of neutrophils?
What is the function of neutrophils?
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What makes neutrophils good at fighting infection?
What makes neutrophils good at fighting infection?
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What are lysosomes and why are they important in neutrophils?
What are lysosomes and why are they important in neutrophils?
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What is the function of sperm cells?
What is the function of sperm cells?
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What is the role of the sperm cell's head?
What is the role of the sperm cell's head?
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What is the function of the acrosome?
What is the function of the acrosome?
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What is the role of the midpiece in a sperm cell?
What is the role of the midpiece in a sperm cell?
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What is the function of root hair cells?
What is the function of root hair cells?
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How are root hair cells adapted for their function?
How are root hair cells adapted for their function?
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What is the significance of thin walls in root hair cells?
What is the significance of thin walls in root hair cells?
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What is the function of ciliated epithelial cells?
What is the function of ciliated epithelial cells?
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Phagocytosis
Phagocytosis
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Neutrophil Adaptation: Flexibility
Neutrophil Adaptation: Flexibility
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Neutrophil Adaptation: Lysosomes
Neutrophil Adaptation: Lysosomes
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Cambium
Cambium
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Plant Cell Differentiation
Plant Cell Differentiation
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Xylem Cell Differentiation
Xylem Cell Differentiation
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Phloem Cell Differentiation
Phloem Cell Differentiation
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Multipotent Adult Stem Cells
Multipotent Adult Stem Cells
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Nucleus
Nucleus
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Nucleolus
Nucleolus
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Nuclear Envelope
Nuclear Envelope
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Mitochondria
Mitochondria
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Lysosomes
Lysosomes
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Chloroplasts
Chloroplasts
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Plasma Membrane
Plasma Membrane
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Centrioles
Centrioles
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Cell Wall
Cell Wall
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Flagella
Flagella
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Cilia
Cilia
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Vacuole
Vacuole
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Protein Production
Protein Production
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Cytoskeleton
Cytoskeleton
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Prokaryotes
Prokaryotes
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Eukaryotes
Eukaryotes
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Confocal Microscopy
Confocal Microscopy
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Resolution
Resolution
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Magnification
Magnification
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Electron Microscope
Electron Microscope
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Scanning Electron Microscope (SEM)
Scanning Electron Microscope (SEM)
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Transmission Electron Microscope (TEM)
Transmission Electron Microscope (TEM)
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Eyepiece Graticule
Eyepiece Graticule
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Stage Micrometer
Stage Micrometer
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Staining
Staining
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Focusing
Focusing
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Study Notes
Cell Cycle
- The cell cycle is a precisely controlled process with three phases: interphase, nuclear division (mitosis), and cell division (cytokinesis).
- Interphase: Cell growth, normal function, and DNA replication for mitosis.
- Consists of three phases: G1 (growth), S (DNA synthesis), and G2 (growth and error checking).
- G1 is when cells produce RNA, enzymes, and other proteins.
- S phase replicates DNA, creating sister chromatids.
- G2 checks newly synthesised DNA for errors and makes necessary repairs.
- Mitosis: Nuclear division creating identical daughter nuclei.
- Divided into four stages: prophase, metaphase, anaphase, and telophase.
- Prophase: Chromosome condensation, centrosome movement, spindle fibre emergence, nuclear envelope breakdown, and nucleolus disappearance.
- Metaphase: Centrosomes at poles, spindle fibres extend and attach to chromosomes at kinetochores, chromosomes line up at metaphase plate.
- Anaphase: Sister chromatids separate, spindle fibers shorten and pull separated chromatids (now chromosomes) to opposite poles.
- Telophase: Chromosomes decondense, nuclear envelopes reform, spindle fibers break down, and nucleoli reform.
- Cytokinesis: Full cell division creating two identical daughter cells.
- Animal cells: Cytoplasm constriction.
- Plant cells: New cell wall formation.
- Cell Cycle Length: Varies based on environmental conditions, cell type, and organism.
- Onion root tip cells divide roughly every 20 hours.
- Human intestine epithelial cells divide roughly every 10 hours.
- Cell Cycle Regulation: Chemical signals called cyclins trigger transitions between phases. Checkpoints ensure accurate DNA replication and prevent errors.
- Four checkpoints: G1 (DNA damage), S (replication completeness), G2 (DNA damage), Metaphase (chromosome attachment).
- Flow Cytometry: Measures DNA mass in cells during the cell cycle.
Mitosis in Plant Cells
- Mitosis in plants is studied in actively dividing meristem regions (e.g., root tips).
- Techniques: Pre-prepared or temporary slides (squash technique) using stains (e.g., acetic orcein).
- Limitations: Difficulty in distinguishing prophase and telophase; inconsistent cell and tissue sizes; low magnification of simple microscopes.
Significance of Mitosis
- Growth of multicellular organisms: Creates genetically identical cells for growth.
- Cell replacement & tissue repair: Replaces damaged or dying cells with identical ones.
- Asexual reproduction: Creates genetically identical offspring in unicellular and multicellular organisms.
Meiosis
- Meiosis: Nuclear division producing haploid cells from diploid cells; used in sexual reproduction.
- Two divisions: Meiosis I and Meiosis II.
- Meiosis I: Homologous chromosomes pair, crossover, separate.
- Prophase I: Homologous chromosome pairing, crossover, centriole migration, spindle formation, nuclear envelope breakdown, and nucleolus disintegration.
- Metaphase I: Homologous pairs align at the metaphase plate, independent assortment.
- Anaphase I: Homologous pairs separate.
- Telophase I: Chromosomes at poles, spindle fibers break, nuclear envelopes reform, and nucleoli reform.
- Meiosis II: Similar to mitosis; sister chromatids separate, resulting in four haploid cells.
- Crossing Over: Exchange of alleles between non-sister chromatids during Prophase I. Creates genetic variation.
- Independent Assortment: Random alignment of homologous chromosomes during Metaphase I, adding to variation.
- Random Fusion of Gametes: Fusion of any gamete increases genetic variation between zygotes.
Specialised Cells
- Eukaryotic cells specialize for specific functions in complex organisms.
- Adaptations: Shape, organelles, and absence of certain organelles.
- Examples: Erythrocytes (oxygen transport), Neutrophils (phagocytosis), Sperm cells (reproduction), Root hair cells (water absorption), Ciliated epithelium (substance movement), Squamous epithelium (diffusion), Palisade cells (photosynthesis), Guard cells (stomata regulation), Xylem vessels (water transport), Phloem cells (sugar transport), Muscle cells (movement), Cartilage (support).
Stem Cells
- Stem cells: Divide indefinitely and differentiate into specialized cells.
- Potency:
- Totipotent: Differentiate into any cell type.
- Pluripotent: Differentiate into any cell type in an embryo.
- Multipotent: Differentiate into a limited range of specialized cells.
- Uses in research and medicine: Stem cells offer potential for disease treatment and injury repair.
- Multipotent stem cells: Found in bone marrow and used for blood cell production.
Eukaryotic Cell Ultrastructure
- Organelles: Nucleus, nucleolus, nuclear envelope, rough ER, smooth ER, Golgi apparatus, ribosomes, mitochondria, lysosomes, chloroplasts, plasma membrane, centrioles (animal cells only), cell wall, flagella, cilia, vacuole, cytoskeleton.
- Functions: Protein synthesis, energy production, waste disposal, photosynthesis, etc.
- Prokaryotic vs. Eukaryotic Cells: Key differences include absence of membrane-bound organelles & nuclear envelope in Prokaryotes, distinct DNA structure, size, and cell wall composition
Microscopy
- Techniques: Light microscopy, Transmission electron microscopy (TEM), Scanning electron microscopy (SEM).
- Applications: Visualizing cells, organelles, and tissues. High-resolution, color & 3-D image capture & analysis are possible.
- Magnification and Resolution: Key concepts for evaluating images.
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