10 Questions
What is the purpose of cryopreservation in cell culture?
To preserve cells at very low temperatures for long-term storage
What distinguishes in vivo from in vitro cell culture?
In vivo refers to cells growing in the body, while in vitro refers to cells growing on plastic or glass
What are the applications of cell lines in cell culture?
Studying the activity of normal and pathological cells in isolation
What is the primary role of maintaining cultures of animal cells and established cell lines in cell culture?
To ensure good viability and minimal contamination
What is the key feature of the special media used in cell culture?
It contains nutrients to support cell growth and division
What is the primary purpose of cryopreservation in cell culture?
To preserve cells for future use and maintain their viability
How does in vivo cell culture differ from in vitro cell culture?
In vivo refers to cells growing in the body, while in vitro refers to cells growing on plastic or glass in a controlled environment
What distinguishes mammalian cultures from bacterial cultures?
Mammalian cultures require specialized growth conditions, while bacterial cultures can grow in simpler environments
What are the key features of the special media used in cell culture?
They contain essential nutrients to support cell growth and division
What is the significance of maintaining cultures of animal cells and established cell lines with good viability and minimal contamination?
To ensure reliable and reproducible experimental results
Study Notes
Cell Culture Basics
- Cryopreservation in cell culture allows for the long-term storage of cells, preserving their viability and characteristics for future use.
In Vivo vs. In Vitro Cell Culture
- In vivo cell culture refers to the growth of cells within a living organism, whereas in vitro cell culture involves the growth of cells outside of a living organism, in a controlled laboratory environment.
- The main difference between in vivo and in vitro cell culture lies in the presence of a living organism, with in vitro cultures being more controlled and manipulated.
Applications of Cell Lines
- Cell lines are used in cell culture to study cellular biology, develop and test new therapies, and produce biological products, such as vaccines and antibodies.
Maintaining Cell Cultures
- The primary role of maintaining cultures of animal cells and established cell lines is to ensure a steady supply of cells for research, development, and production purposes.
- Maintaining cultures with good viability and minimal contamination is crucial for obtaining reliable and accurate results.
Special Media in Cell Culture
- The special media used in cell culture are designed to support the growth and survival of cells, with key features including a controlled pH, temperature, and nutrient composition.
- These media may also include supplements, such as antibiotics and growth factors, to enhance cell growth and survival.
Test your knowledge of cell culture techniques in biotechnology with this quiz. Assess your understanding of tasks such as media preparation, culture maintenance, contamination prevention, and cryopreservation. Distinguish between mammalian and established cell lines to enhance your skill set in biotechniques.
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