Podcast
Questions and Answers
What is the difference between primary culture and sub-culture?
What is the difference between primary culture and sub-culture?
Match the following cell types to their descriptions:
Match the following cell types to their descriptions:
Fibroblast-like = Elongated & spindle-shaped Epithelial-like = Polygonal & tightly packed Lymphoblast-like = Round & in suspension
What is cell culture?
What is cell culture?
Growing cells in controllable conditions
What is the optimal temperature for the growth of human and mammalian cell lines?
What is the optimal temperature for the growth of human and mammalian cell lines?
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What are the steps of the cell culture process?
What are the steps of the cell culture process?
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What is cell culture?
What is cell culture?
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What is the difference between primary culture and sub-culture?
What is the difference between primary culture and sub-culture?
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Match the following cell types with their appearances:
Match the following cell types with their appearances:
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What are the steps of the cell culture process?
What are the steps of the cell culture process?
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What is the humidity percentage that limits the evaporation of water from the media?
What is the humidity percentage that limits the evaporation of water from the media?
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What is the temperature for optimal growth of human and mammalian cell lines?
What is the temperature for optimal growth of human and mammalian cell lines?
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What are the CO2 percentage levels that are common in most cell culture experiments to balance pH?
What are the CO2 percentage levels that are common in most cell culture experiments to balance pH?
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What is the pH for mammalian cell lines provided by culture medium?
What is the pH for mammalian cell lines provided by culture medium?
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What is the purpose of aseptic techniques?
What is the purpose of aseptic techniques?
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What are the different types of aseptic techniques?
What are the different types of aseptic techniques?
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Match the following color changes with their corresponding pH levels:
Match the following color changes with their corresponding pH levels:
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What is the pH indicator used for cell culture media?
What is the pH indicator used for cell culture media?
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What are the risks of improper aseptic techniques?
What are the risks of improper aseptic techniques?
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What often signifies deterioration in a cell culture?
What often signifies deterioration in a cell culture?
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Study Notes
Cell Culture Overview
- Cell culture involves cultivating cells under controlled environmental conditions to study their behavior and growth.
Types of Cell Culture
- Primary Culture: Derived directly from animal or plant tissues, representing the initial growth stage.
- Sub-Culture: Involves transferring cells from one culture vessel to another, supporting their continued growth and replication.
Cell Culture Process Steps
- Tolation: Preparation of tissues for culturing, typically involving disaggregation.
- Sterilization: Ensures that the culture environment is free from contamination by microorganisms.
- Growth: Cells proliferate within the nutrient-rich medium provided.
- Subculture: Regularly transferring some cells to new vessels to maintain optimal density and health.
- Harvesting: Collecting cells for analysis or further experimentation once they reach desired growth levels.
Cell Morphology
- Fibroblast-like Cells: Characterized by an elongated and spindle-shaped appearance, often involved in the formation of connective tissues.
- Epithelial-like Cells: Exhibit a polygonal and tightly packed structure, commonly found in interfaces such as skin and lining of organs.
- Lymphoblast-like Cells: Typically round and suspended within the growth medium, representing a stage in lymphocyte development.
Incubator Requirements
- Optimal temperature for growth of human and mammalian cell lines is maintained at 37∘C37^\circ C37∘C, mirroring physiological conditions.
Cell Culture Overview
- Cell culture involves cultivating cells under controlled environmental conditions to study their behavior and growth.
Types of Cell Culture
- Primary Culture: Derived directly from animal or plant tissues, representing the initial growth stage.
- Sub-Culture: Involves transferring cells from one culture vessel to another, supporting their continued growth and replication.
Cell Culture Process Steps
- Tolation: Preparation of tissues for culturing, typically involving disaggregation.
- Sterilization: Ensures that the culture environment is free from contamination by microorganisms.
- Growth: Cells proliferate within the nutrient-rich medium provided.
- Subculture: Regularly transferring some cells to new vessels to maintain optimal density and health.
- Harvesting: Collecting cells for analysis or further experimentation once they reach desired growth levels.
Cell Morphology
- Fibroblast-like Cells: Characterized by an elongated and spindle-shaped appearance, often involved in the formation of connective tissues.
- Epithelial-like Cells: Exhibit a polygonal and tightly packed structure, commonly found in interfaces such as skin and lining of organs.
- Lymphoblast-like Cells: Typically round and suspended within the growth medium, representing a stage in lymphocyte development.
Incubator Requirements
- Optimal temperature for growth of human and mammalian cell lines is maintained at 37∘C37^\circ C37∘C, mirroring physiological conditions.
Humidity and CO2 Levels
- Humidity level of 95% is crucial to limit water evaporation from culture media.
- Common CO2 concentration of 5% is used in cell culture to help balance pH levels.
- Ideal pH range for mammalian cell lines in culture medium is between 7.2 and 7.4.
Cell Culture Lab Setup
- Essential equipment includes a biosafety cabinet for safe handling and a waste disposal system to manage biohazard materials.
- Microscopes and cell culture hoods are necessary for observation and sterile working conditions.
- Incubators maintain optimal temperature and CO2 levels; sterilization equipment ensures a contamination-free environment.
- Centrifuges are used for cell separation; media supplements provide necessary nutrients for cell growth.
- Refrigerators store media and biological samples at controlled temperatures.
- Culture vessels provide a suitable environment for cells; pipettes are used for precise liquid handling.
- Cryopreservation equipment is vital for the long-term storage of biological samples.
Aseptic Technique
- Aseptic techniques are employed to prevent contamination in cell culture.
- Various methods include using disinfectants, wearing personal protective equipment (PPE), and practicing sterile handling techniques.
- Failure to adhere to aseptic techniques can lead to contamination, which can compromise experimental results.
pH Indicator for Cell Culture Media
- Phenol Red is the pH indicator used in cell culture media.
- Color change indicates pH levels:
- Red indicates neutral pH.
- Yellow indicates acidic conditions.
- Pink signifies basic conditions.
Mycoplasma Detection
- Presence of Mycoplasma is often the initial indicator of cell culture deterioration.
- Monitoring for Mycoplasma is essential for maintaining culture quality and integrity.
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Description
Explore the fundamentals of cell culture, including the types, processes, and key techniques involved in cultivating cells. Learn about primary and sub-cultures, as well as essential steps like sterilization and harvesting for experimentation.
