Cell Biology Quiz on Bacterial Structures

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Questions and Answers

What is the primary function of chaperones in cells?

  • To provide energy to the cell
  • To transport substances across membranes
  • To assist in protein folding (correct)
  • To catalyze metabolic reactions

Polysomes are structures formed only in eukaryotic cells during translation.

False (B)

What are second messengers and provide an example of one that is used in bacteria?

Second messengers are small molecules that relay signals inside cells; an example is cyclic AMP (cAMP).

In quorum sensing, bacteria communicate through the release of __________ to coordinate behavior.

<p>signaling molecules</p> Signup and view all the answers

Match the following gene regulation terms with their functions:

<p>Activator = Enhances gene expression Repressor = Inhibits gene expression Inducer = Triggers activation of a gene Co-repressor = Works with a repressor to inhibit gene expression</p> Signup and view all the answers

Which of the following structures are included in the term 'cell envelope'?

<p>All of the above (D)</p> Signup and view all the answers

All life forms have a cell membrane.

<p>True (A)</p> Signup and view all the answers

What primary function does a cell membrane serve in bacterial cells?

<p>Barrier</p> Signup and view all the answers

Bacterial cell walls are primarily made of __________.

<p>peptidoglycan</p> Signup and view all the answers

What is a characteristic of Gram-positive bacteria cell walls compared to Gram-negative bacteria?

<p>They have a thicker peptidoglycan layer. (D)</p> Signup and view all the answers

What are teichoic acids, and how are they related to Gram-positive bacteria?

<p>Teichoic acids are components of Gram-positive bacterial cell walls that provide structural support.</p> Signup and view all the answers

Match the types of membrane proteins with their descriptions:

<p>Integral = Span the entire membrane Peripheral = Attach temporarily to the surface Transmembrane = Cross the membrane multiple times Amphipathic = Have both hydrophilic and hydrophobic regions</p> Signup and view all the answers

Peptidoglycan is impermeable to most substances and does not allow any penetration.

<p>False (B)</p> Signup and view all the answers

Which of the following scientists is known for disproving the concept of spontaneous generation?

<p>Louis Pasteur (C)</p> Signup and view all the answers

Koch's Postulates can be applied only to non-infectious diseases.

<p>False (B)</p> Signup and view all the answers

What are the three domains of life?

<p>Bacteria, Archaea, Eukarya</p> Signup and view all the answers

The process of using a gel-based medium to isolate pure microbial cultures is often referred to as _______.

<p>streaking</p> Signup and view all the answers

Match the microbiological pioneers to their key contributions:

<p>Louis Pasteur = Germ theory of disease Robert Koch = Koch's Postulates Anton van Leeuwenhoek = Microscopy advancements Joseph Lister = Antiseptic surgical practices</p> Signup and view all the answers

What was the first genetic material according to the RNA world hypothesis?

<p>RNA (C)</p> Signup and view all the answers

Microbes are only found in specific environments such as soil and water.

<p>False (B)</p> Signup and view all the answers

What is the importance of microscopy in microbiology?

<p>Microscopy allows for the visualization of microbes, which were previously invisible to the naked eye, facilitating the study of their structure and behavior.</p> Signup and view all the answers

Which component of lipopolysaccharide (LPS) is primarily responsible for its toxic properties?

<p>Lipid A (B)</p> Signup and view all the answers

All bacteria possess surface layers (S layers).

<p>False (B)</p> Signup and view all the answers

What is the primary difference between capsules and slime layers in bacteria?

<p>Capsules are tightly bound to the bacterial cell and structured, while slime layers are loosely attached and unstructured.</p> Signup and view all the answers

The __________ transport system utilizes energy to move molecules against their concentration gradient.

<p>active</p> Signup and view all the answers

Match the following components with their correct functions:

<p>Porins = Facilitate the movement of molecules across the outer membrane Braun’s lipoprotein = Anchors the outer membrane to the peptidoglycan layer Capsule = Protects against phagocytosis and aids in attachment Pili = Facilitates attachment and conjugation between bacteria</p> Signup and view all the answers

What is the role of the periplasmic space in Gram-negative bacteria?

<p>It contains enzymes and proteins for nutrient processing (A)</p> Signup and view all the answers

Facilitated diffusion requires energy to transport molecules across the cytoplasmic membrane.

<p>False (B)</p> Signup and view all the answers

What is the difference between symport and antiport transport systems?

<p>Symport moves two molecules in the same direction, while antiport moves two molecules in opposite directions.</p> Signup and view all the answers

Which organism would be classified as a thermophile?

<p>Archaeon found in hot springs (D)</p> Signup and view all the answers

Psychrophiles can only survive at temperatures above 0°C.

<p>False (B)</p> Signup and view all the answers

What major challenge do acidophiles face in extreme pH environments?

<p>Maintaining internal pH levels suitable for cellular function.</p> Signup and view all the answers

Organisms that can survive or grow in high salt concentrations are known as ______.

<p>halophiles</p> Signup and view all the answers

What does the term 'decimal reduction time' refer to?

<p>The time required to reduce microbial populations by 90% (D)</p> Signup and view all the answers

Obligate anaerobes thrive in the presence of oxygen.

<p>False (B)</p> Signup and view all the answers

Name one adaptation that allows thermophiles to survive in high temperatures.

<p>Heat-stable enzymes.</p> Signup and view all the answers

Match the following types of microorganisms with their characteristics:

<p>Obligate Aerobes = Require oxygen for growth Facultative Anaerobes = Can grow with or without oxygen Obligate Anaerobes = Cannot tolerate oxygen Microaerophilic Organisms = Require low levels of oxygen</p> Signup and view all the answers

Which of the following is NOT a type of symbiotic relationship?

<p>Absorption (B)</p> Signup and view all the answers

Obligate symbionts can survive without their host organisms.

<p>False (B)</p> Signup and view all the answers

What is a lichen?

<p>A lichen is a symbiotic association between a fungus and a photosynthetic partner, often algae or cyanobacteria.</p> Signup and view all the answers

The production of antibiotics by Streptomyces species is believed to be a form of ______ warfare.

<p>microbial</p> Signup and view all the answers

Match the following terms with their corresponding definitions:

<p>T6SS = A mechanism used by bacteria to inject toxic molecules into target cells Bacteriocins = Antimicrobial peptides produced by bacteria to kill similar or closely related bacterial strains Killer yeast = Yeasts that produce compounds to inhibit or kill competing microorganisms Amoebae = Single-celled organisms that consume bacteria</p> Signup and view all the answers

How do organisms benefit from the symbiotic relationship in photosynthetic consortia?

<p>The photosynthetic partner provides energy, while the other partner offers protection (B)</p> Signup and view all the answers

Legionella pneumophila is thought to have evolved its virulence due to its relationship with predatory amoebae.

<p>True (A)</p> Signup and view all the answers

What are the primary targets of type VI secretion systems (T6SS)?

<p>T6SS typically targets other bacterial cells.</p> Signup and view all the answers

Flashcards

Spontaneous Generation

The idea that living organisms can arise spontaneously from non-living matter.

Importance of Microscopy in Microbiology

Microscopes allowed scientists to observe and study microbes, leading to numerous discoveries in microbiology.

Pasteur's Contributions

Pasteur demonstrated that microbes are responsible for fermentation and disease, and developed pasteurization.

Koch's Contributions

Koch established the link between specific microbes and specific diseases.

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Koch's Postulates

A set of criteria used to establish a causal relationship between a microorganism and a disease.

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Isolating Pure Cultures

The process of isolating and growing a pure culture of a single type of microbe.

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Domains of Life

The three domains of life are Bacteria, Archaea, and Eukarya.

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RNA World Hypothesis

The hypothesis that RNA was the first genetic material, preceding DNA.

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Polysome

A complex of multiple ribosomes translating the same mRNA molecule simultaneously, enhancing protein synthesis efficiency.

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Transcriptional/Translational Coupling

A process where translation begins while transcription is still ongoing, enabling rapid protein production in prokaryotes where both processes occur in the cytoplasm.

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Chaperones

Proteins that assist in proper protein folding and prevent misfolded proteins from aggregating, crucial for maintaining cellular function and preventing disease.

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Protein Translocation

The movement of newly synthesized proteins from the site of synthesis (ribosomes) to their specific destinations within the cell (e.g., organelles, cell membrane), ensuring proper function and preventing mislocalization.

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Sec Secretion System

A protein secretion system in bacteria that translocates proteins across the cell membrane, with two main branches: SecA-dependent pathway for folded proteins and SecYEG-dependent pathway for unfolded proteins.

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Lipopolysaccharide (LPS)

A component of the outer membrane of Gram-negative bacteria, composed of lipid A, core polysaccharide, and O-specific polysaccharide. It is recognized by the immune system and can trigger a strong inflammatory response, leading to sepsis in severe cases.

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Lipid A

The innermost part of LPS, deeply embedded in the outer membrane. It is a hydrophobic molecule consisting of two glucosamine sugars with fatty acyl chains attached.

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How LPS is used to classify bacteria

The variability in the O-specific polysaccharide of LPS, which is a chain of sugar molecules, allows for classification of different bacterial species.

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Porin

A transport protein embedded in the outer membrane of Gram-negative bacteria that facilitates the passage of small hydrophilic molecules, such as sugars and amino acids.

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Braun's Lipoprotein

A small protein that links the outer membrane to the peptidoglycan layer in Gram-negative bacteria. It helps maintain the structural integrity of the cell envelope.

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Periplasmic Space

A region located between the inner and outer membranes of Gram-negative bacteria, containing peptidoglycan and several important enzymes and proteins. It plays a vital role in metabolism, transport, and defense.

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Symport

A type of active transport system where the energy released by the movement of one molecule down its concentration gradient is used to drive the movement of another molecule against its concentration gradient.

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Antiport

A type of active transport system where the movement of one molecule down its concentration gradient is used to drive the movement of another molecule in the opposite direction.

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Cell envelope

The outer layer of a bacterial cell, which plays a vital role in protection, shape, and interaction with the environment.

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Ubiquity of Cell Membrane

All living organisms, from bacteria to plants and animals, possess a cell membrane that encloses their cytoplasm. Due to their shared evolutionary history, their basic structures resemble each other.

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Primary Function of Cell Membrane

The cell membrane's primary function is to act as a selective barrier controlling what enters and leaves the cell. It also plays crucial roles in energy production, cell signaling, and transport.

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Structure of Bacterial Cytoplasmic Membranes

Bacterial cytoplasmic membranes are phospholipid bilayers, with phospholipids consisting of a hydrophilic head and a hydrophobic tail. These molecules spontaneously arrange themselves in bilayers with hydrophilic heads facing outward and hydrophobic tails pointing inward.

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Importance of Cell Walls in Bacteria

Bacterial cell walls are rigid structures that maintain the shape of the cell and protect it against osmotic stress, which can cause the cell to burst. They primarily consist of peptidoglycan, a unique polymer not found in other domains of life.

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Structure of Peptidoglycan

Peptidoglycan is a complex polymer composed of repeating sugar units linked by short peptides. The sugar backbone consists of alternating N-acetylglucosamine (NAG) and N-acetylmuramic acid (NAM) residues, linked to each other by peptide cross-links.

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Gram-Positive vs. Gram-Negative Cell Walls

Gram-positive bacteria have a thick peptidoglycan layer, while Gram-negative bacteria have a thin peptidoglycan layer and an additional outer membrane. The presence or absence of this outer membrane allows the bacteria to be distinguished by Gram staining, a technique used for bacterial identification.

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Permeability of Peptidoglycan

The peptidoglycan layer is somewhat permeable, allowing small molecules like water and nutrients to pass through. However, it restricts the entry of larger molecules and can inhibit the penetration of certain antibiotics.

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Psychrophile

Organisms that thrive in extremely cold environments, typically below 15°C.

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Mesophile

Organisms that grow optimally at moderate temperatures, typically between 20°C and 45°C.

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Thermophile

Organisms that thrive in extremely hot environments, typically above 45°C.

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Hyperthermophile

Organisms that thrive in extremely hot environments, typically above 80°C.

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Acidophile

Organisms that thrive in acidic environments, typically with pH values below 3.

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Alkaliphile

Organisms that thrive in alkaline environments, typically with pH values above 9.

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Decimal Reduction Time (D-value)

The time required to reduce the number of microbes by 90% at a specific temperature.

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Autoclave

A device that uses high pressure steam to sterilize materials by killing all microorganisms and their spores.

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Symbiosis

A close and often long-term interaction between two different species where at least one species benefits.

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Mutualism

A symbiotic relationship where both organisms benefit from the interaction.

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Commensalism

A symbiotic relationship where one organism benefits while the other is neither harmed nor helped.

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Parasitism

A symbiotic relationship where one organism benefits at the expense of the other.

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Obligate symbiont

An organism that cannot survive without its symbiotic partner.

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Lichen

Complex organisms composed of a fungus (usually ascomycete) and an alga (usually a green alga).

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Predatory organisms

Organisms that consume other organisms. Predators can be single-celled (like amoebae) or multicellular (like lions).

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Microbial consortium

A complex, multi-species microbial community that can thrive in diverse environments.

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Study Notes

Learning Objectives for Topic 1: Introduction to Microbial Life

  • For most of human history, microbes and their influence on life were unknown.
  • Microscopy was crucial in microbiology's development, along with contributions from early pioneers.
  • The idea of spontaneous generation was disproven through key experiments.
  • Prominent microbiologists like Pasteur and Koch made significant contributions.
  • Koch's Postulates are used to determine the cause of infectious disease (though they have limitations).
  • Isolate microbial cultures are important in advancing the field of microbiology.

Learning Objectives for Topic 2: Structure & Function of Microbial Cells

  • Prokaryotic and eukaryotic cells differ in structure (e.g., the presence of a nucleus).
  • Cell size varies, but size impacts surface-to-volume ratio and growth.
  • Prokaryotic cell shapes (morphologies) are diverse and important in many aspects of their biology.
  • Key prokaryotic shapes include cocci, bacilli, and spirilla.
  • Cell envelopes include membranes, walls and layers.
  • Bacterial cytoplasmic membranes consist of phospholipids and proteins, serving as a barrier and carrying out several other functions.
  • The cell wall provides structure in prokaryotes; gram-negative versus gram-positive are important.
  • Peptidoglycan structure and permeability in cell walls.
  • Gram-positive and negative cells differ.
  • Outer membranes are present in Gram-negative bacteria.
  • LPS structure and properties.
  • Bacterial outer membranes.
  • Bacterial cell inclusions, endospores, and their functions.

Learning Objectives for Topic 3: Genomes, Genetics & Genomics

  • Nucleic acid structure (e.g., nucleotides, purines, pyrimidines).
  • Prokaryotic chromosomes and organization, compared with eukaryotes.
  • Genome composition (genes, non-coding regions).
  • Typical prokaryotic genome size.
  • Core genome and pan genome.
  • Genomic islands, prophages, plasmids.
  • Prokaryotic genetics terms.
  • Horizontal gene transfer (transformation, transduction, conjugation).
  • p(Tn) insertion sequences in bacterial DNA,
  • DNA Sequencing technology.
  • Sanger vs Next generation sequencing.
  • Metagenomics and RNA-seq.

Learning Objectives for Topic 4: Gene Expression & Regulation

  • Transcription, its role in gene expression.
  • RNA polymerase and sigma factors (housekeeping and specialized).
  • Transcription sequences (upstream/downstream).
  • RNA polymerase structure and function.
  • Transcriptional elongation and termination mechanisms.
  • Bacterial mRNA structure (e.g., 5' and 3' UTRs).
  • Bacterial transcription compared to archaeal and eukaryotic transcription.
  • Translation and protein structure and composition.
  • Different types of tRNA, mRNA, and ribosome function.
  • Differences in eukaryotic and prokaryotic translation.
  • Transcriptional regulation aspects, mechanisms and factors.

Learning Objectives for Topic 5: Energetics & Metabolism

  • Enzymes and their role in metabolic reactions, facilitating reactions by lowering activation energy.
  • Enzyme regulation (e.g., competitive inhibition and allosteric regulation).
  • The role of ATP in many metabolic pathways.
  • Importance of microbes in the context of metabolism.
  • Nutrients, ATP, metabolic processes in cells.
  • Redox reactions, electron donors and acceptors, reduction potentials.
  • Gibbs free energy.
  • Chemoorganotrophs and chemolithotrophs.
  • Redox (redox reactions) and ATP.
  • Different types of respiration and fermentation.
  • Catabolism (e.g. glycolysis, citric acid cycle, electron transport chain, oxidative phosphorylation; fermentation).
  • Key metabolic processes for microorganisms.
  • Carbon and energy utilization.
  • Photosynthesis, biosynthetic pathways.
  • Nitrogen fixation.
  • Microbial metabolisms.

Learning Objectives for Topic 6: Microbial Growth & Growth Control

  • Binary fission, steps in bacterial cell division.
  • Cell division mechanisms.
  • Divisomes and their function.
  • Microbial growth and cell division.
  • Factors influencing microbial growth including pH and temperature.
  • Culturing methods (selective, differential and enrichment).
  • Microbial growth phases in culture.
  • Microbial growth control and heat/pressure sterilization.
  • Factors that impact microbial growth.
  • Microbial cultures and cell divison.
  • Isolation and culture methods.
  • Biofilms, and their structure and function.

Learning Objectives for Topic 7: Microbial Communities & Interactions

  • Fundamental and realized niche concept.
  • Symbiosis and its different types (mutualistic, commensal, parasitic).
  • Lichens and photosynthetic consortia.
  • Microbial communities (e.g., in soil).
  • Microbial Interactions.
  • Methods to inhibit or kill neighboring microbes.
  • Bacteriocins and antibiotics.

Learning Objectives for Topic 8: Microbes in Health & Disease

  • Human microbiome; composition and diversity.
  • Microbiome composition and how they vary in health and disease.
  • Gut microbiota, and gut microbiota interactions.
  • Human microbiome and other microbes in humans - skin, gut and bacteria in humans.
  • Microbial pathogens and virulence factors.
  • Pathogenesis of microbes.
  • Microbial pathogen mechanisms and how they cause disease.
  • Effects and responses to microbial infections.
  • Microbial pathogenesis, including exotoxins, endotoxins and viral toxins.

Learning Objectives for Additional Topics in the Provided Text

  • Antibiotic resistance.
  • Early antibiotic discovery
  • Microbial toxins.
  • Specific bacterial examples and diseases.

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