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Questions and Answers
Which of these organelles is NOT found in animal cells, but is found in plant cells?
Which of these organelles is NOT found in animal cells, but is found in plant cells?
What is the function of the endoplasmic reticulum?
What is the function of the endoplasmic reticulum?
Which organelle is responsible for packaging and modifying proteins?
Which organelle is responsible for packaging and modifying proteins?
What is the function of lysosomes?
What is the function of lysosomes?
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Which of these is NOT a component of the endomembrane system?
Which of these is NOT a component of the endomembrane system?
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Which of these cellular components is responsible for providing structural support and movement within a eukaryotic cell?
Which of these cellular components is responsible for providing structural support and movement within a eukaryotic cell?
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What is the main function of ribosomes?
What is the main function of ribosomes?
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Which of these organelles contains its own DNA?
Which of these organelles contains its own DNA?
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What is the function of the nucleolus?
What is the function of the nucleolus?
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What is the primary component of chromatin?
What is the primary component of chromatin?
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What is the function of the nuclear envelope?
What is the function of the nuclear envelope?
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What is the name of the distinct units that chromatin condenses into during cell division?
What is the name of the distinct units that chromatin condenses into during cell division?
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Which of these is NOT a function of the nuclear pores?
Which of these is NOT a function of the nuclear pores?
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Based on the provided text, why are eukaryotic cells called eukaryotic?
Based on the provided text, why are eukaryotic cells called eukaryotic?
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If you were to uncoil and lay out end-to-end the DNA from a single human cell, how long would it be?
If you were to uncoil and lay out end-to-end the DNA from a single human cell, how long would it be?
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Which of the following statements is TRUE regarding the DNA in the human body?
Which of the following statements is TRUE regarding the DNA in the human body?
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What is a signal peptide, and what does it do?
What is a signal peptide, and what does it do?
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Which of the following is NOT a correct statement about ribosomes?
Which of the following is NOT a correct statement about ribosomes?
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What is the role of the endoplasmic reticulum (ER) in protein synthesis?
What is the role of the endoplasmic reticulum (ER) in protein synthesis?
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What would happen if a protein's signal sequence was altered or removed?
What would happen if a protein's signal sequence was altered or removed?
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What is the function of a nuclear localization signal (NLS)?
What is the function of a nuclear localization signal (NLS)?
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Which of the following is a characteristic of a signal sequence that directs a protein to the peroxisome?
Which of the following is a characteristic of a signal sequence that directs a protein to the peroxisome?
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Where is the nucleolus located?
Where is the nucleolus located?
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What is the function of the Golgi apparatus in protein synthesis?
What is the function of the Golgi apparatus in protein synthesis?
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Which of the following is NOT a component of the endomembrane system?
Which of the following is NOT a component of the endomembrane system?
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What is the primary function of the rough endoplasmic reticulum?
What is the primary function of the rough endoplasmic reticulum?
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How does the smooth endoplasmic reticulum differ from the rough endoplasmic reticulum?
How does the smooth endoplasmic reticulum differ from the rough endoplasmic reticulum?
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Which of the following is a primary function of the Golgi apparatus?
Which of the following is a primary function of the Golgi apparatus?
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Which of the following is the most likely destination of a protein synthesized on the rough endoplasmic reticulum?
Which of the following is the most likely destination of a protein synthesized on the rough endoplasmic reticulum?
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Which of the following is a key difference between the nuclear envelope and the endoplasmic reticulum?
Which of the following is a key difference between the nuclear envelope and the endoplasmic reticulum?
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How do transport vesicles move between different components of the endomembrane system?
How do transport vesicles move between different components of the endomembrane system?
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What is the main function of the endoplasmic reticulum (ER) in the synthesis of insulin?
What is the main function of the endoplasmic reticulum (ER) in the synthesis of insulin?
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What is the role of the Golgi apparatus in the processing of insulin?
What is the role of the Golgi apparatus in the processing of insulin?
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What is the function of the signal recognition particle (SRP) in the synthesis of insulin?
What is the function of the signal recognition particle (SRP) in the synthesis of insulin?
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What is the main function of mitochondria?
What is the main function of mitochondria?
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What is the role of the lysosomes in the cell?
What is the role of the lysosomes in the cell?
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Which of the following is NOT an evidence supporting the endosymbiotic theory?
Which of the following is NOT an evidence supporting the endosymbiotic theory?
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What is the name of the space within the chloroplast where the Calvin cycle takes place?
What is the name of the space within the chloroplast where the Calvin cycle takes place?
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What is the process by which insulin is released from the cell?
What is the process by which insulin is released from the cell?
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What is the role of the signal peptide in the synthesis of insulin?
What is the role of the signal peptide in the synthesis of insulin?
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Which of the following organelles is involved in the process of detoxification?
Which of the following organelles is involved in the process of detoxification?
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What is the relationship between insulin and diabetes mellitus?
What is the relationship between insulin and diabetes mellitus?
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What is the name of the stacks of thylakoids in chloroplasts?
What is the name of the stacks of thylakoids in chloroplasts?
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What is the name of the process by which mitochondria and chloroplasts reproduce?
What is the name of the process by which mitochondria and chloroplasts reproduce?
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Which of the following is NOT a function of the Golgi apparatus?
Which of the following is NOT a function of the Golgi apparatus?
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Which of the following statements is TRUE about the endosymbiotic theory?
Which of the following statements is TRUE about the endosymbiotic theory?
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What is the role of the inner membrane of the mitochondria?
What is the role of the inner membrane of the mitochondria?
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Study Notes
Biology 1 - Cells, Molecular Biology, and Genetics (Biol 1000)
- Course offered by Dr. Michael Cardinal-Aucoin in Winter 2025 at York University
- Course focuses on cells, molecular biology, and genetics.
The Eukaryotic Cell
- Eukaryotic cells have internal membranes forming compartments called organelles.
- Each organelle is specialized for a particular task.
- Plant, animal, and protist cells generally share the same organelles.
- Organelles include: nucleus, endomembrane system (endoplasmic reticulum, Golgi apparatus, lysosomes, vacuoles), mitochondria, chloroplasts, peroxisomes, and cytoskeleton.
Eukaryotes
- Eukaryotic cells have internal membranes that divide the cell into compartments (organelles) to help perform specific functions.
- Plant, animal and protist cells have similar organelles.
Nucleus
- Stores the DNA.
- The nuclear envelope encloses the nucleus with an inner and outer membrane.
- Nuclear pores in the envelope allow molecules to move in and out of the nucleus.
- The nucleolus is a dense region where rRNA (ribosomal RNA) is synthesized. Ribosomes are assembled here.
- DNA in the nucleus is organized into chromatin, which is DNA associated with proteins (like histones).
- During cell divison, chromatin condenses to form chromosomes (distinct units).
Nuclear Pore
- Controls traffic into and out of the nucleus.
- Multi-protein structure.
- Allows molecules like proteins and building blocks of DNA and RNA to enter or exit.
Ribosomes
- Present in both prokaryotes and eukaryotes.
- In eukaryotes, ribosomes are assembled in the nucleolus.
- Made up of proteins and rRNA.
- Some are free in the cytosol, others are bound to the endoplasmic reticulum membrane.
- Sites of protein synthesis; amino acids are assembled into proteins.
Protein Synthesis
- Proteins synthesized by free and bound ribosomes have different fates.
- Free ribosomes make proteins for use within the cytosol.
- Bound ribosomes make proteins that are destined for secretion or for use within organelles like the endoplasmic reticulum or Golgi.
- Proteins have signal sequences that direct them to the correct location.
Endomembrane System
- Composed of internal membranes that communicate via direct connections or via vesicles.
- Key components include nuclear envelope, endoplasmic reticulum (rough ER and smooth ER), Golgi apparatus, lysosomes, vacuoles, and plasma membrane.
Endoplasmic Reticulum (ER)
- A network of membranes, continuous with the nuclear envelope.
- Accounts for more than half the total membrane in eukaryotic cells.
- Two distinct regions differ in structure and function: rough ER and smooth ER.
- Rough ER: studded with ribosomes; synthesizes proteins destined for secretion or for use within other organelles; synthesizes membrane components.
- Smooth ER: lacks ribosomes; synthesizes lipids, metabolizes carbohydrates, detoxes drugs and poisons; stores calcium ions.
- The ER is involved in protein synthesis, lipid synthesis, and calcium storage.
Golgi Apparatus
- Looks like flattened membrane sacs (cisternae) and vesicles surrounding the cisternae.
- Not physically connected to the ER.
- Modifies, sorts, and packages proteins and lipids.
- Acts as a sorting station for proteins and lipids.
Lysosomes
- Specialized vesicles derived from the Golgi body.
- Filled with hydrolytic enzymes that break down damaged or unneeded macromolecules.
- Keeps pH around 5; optimal for enzymes to function.
- Important to cellular digestion, and recycling.
Vacuoles
- Large vesicles derived from the ER and Golgi.
- Perform diverse functions, including storing nutrients, acting as lysosomes in some plants, and maintaining turgor pressure in plant cells.
Mitochondria
- Sites of cellular respiration.
- Enclosed by two membranes; outer is smooth, inner is folded into cristae.
- Inner membrane divides the mitochondrion into intermembrane space and mitochondrial matrix, where enzymes, mitochondrial DNA and ribosomes are found.
- Critical for producing ATP, the cell's energy currency.
Chloroplasts
- Capture sunlight energy to produce sugars via photosynthesis.
- In plants and some other eukaryotes.
- Enclosed by a double membrane, contains thylakoid membrane with grana and stroma space.
Origins of Mitochondria and Chloroplasts (Endosymbiosis Theory)
- Mitochondria and chloroplasts evolved from free-living prokaryotic organisms.
- These organisms were engulfed by a larger cell and developed a symbiotic relationship, becoming organelles inside of the cell.
- Evidence supports this theory, including their double membranes, their having free ribosomes and circular DNA.
Peroxisomes
- Specialized metabolic compartments bounded by a single membrane. Perform reactions to break down fatty acids and reduce reactive oxygen species.
- Contain enzymes (catalase) to break down hydrogen peroxide (H2O2), which is a byproduct of these processes.
Other Topics
- Specific pages were given to the endomembrane system and its component parts, like insulin and secretion, in more detail.
- The functions and structures of plant and animal cells were outlined in diagram/image pages.
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Description
Test your knowledge on the structure and function of organelles found in eukaryotic cells, particularly focusing on the differences between animal and plant cells. Answer questions about the endoplasmic reticulum, lysosomes, ribosomes, and more to assess your understanding of cellular biology.