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Which of the following is NOT a primary function of the plasma membrane?
Which of the following is NOT a primary function of the plasma membrane?
The plasma membrane is described as 'semi-permeable,' what does this term most accurately describe?
The plasma membrane is described as 'semi-permeable,' what does this term most accurately describe?
What is the role of the plasma membrane in maintaining ion concentration differences?
What is the role of the plasma membrane in maintaining ion concentration differences?
In the context of cellular function, what is the significance of the plasma membrane forming the boundary of the cell?
In the context of cellular function, what is the significance of the plasma membrane forming the boundary of the cell?
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Which statement best describes the relationship between the plasma membrane and the intracellular fluid (ICF) and extracellular fluid (ECF)?
Which statement best describes the relationship between the plasma membrane and the intracellular fluid (ICF) and extracellular fluid (ECF)?
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What is the primary role of carbohydrate markers on the cell membrane?
What is the primary role of carbohydrate markers on the cell membrane?
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Which component of the extracellular matrix is predominantly responsible for providing tensile strength to tissues?
Which component of the extracellular matrix is predominantly responsible for providing tensile strength to tissues?
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What is the main function of elastin within the extracellular matrix?
What is the main function of elastin within the extracellular matrix?
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Which type of cell junction best describes structures that prevent molecules from passing through the intercellular space?
Which type of cell junction best describes structures that prevent molecules from passing through the intercellular space?
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Which of the following best describes the permeability of the plasma membrane?
Which of the following best describes the permeability of the plasma membrane?
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Which of these substances can pass directly through the lipid bilayer of the plasma membrane?
Which of these substances can pass directly through the lipid bilayer of the plasma membrane?
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Which of the following is NOT a transport mechanism across the plasma membrane?
Which of the following is NOT a transport mechanism across the plasma membrane?
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What is the function of fibronectin in the extracellular matrix?
What is the function of fibronectin in the extracellular matrix?
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Which of the following best describes a substance that is considered impermeable to a cell membrane?
Which of the following best describes a substance that is considered impermeable to a cell membrane?
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What are the two primary properties that determine if a substance can permeate a cell membrane without assistance?
What are the two primary properties that determine if a substance can permeate a cell membrane without assistance?
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A substance that can pass through the membrane via simple diffusion must be:
A substance that can pass through the membrane via simple diffusion must be:
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Assisted transport is required for which types of substances to cross a cell membrane?
Assisted transport is required for which types of substances to cross a cell membrane?
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Which of the following is an example of a passive force that drives unassisted transport across a cell membrane?
Which of the following is an example of a passive force that drives unassisted transport across a cell membrane?
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Which of the following is primarily transported across a cell via unassisted means?
Which of the following is primarily transported across a cell via unassisted means?
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What distinguishes active transport from passive transport across a cell membrane?
What distinguishes active transport from passive transport across a cell membrane?
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What is the significance of simple diffusion for cells?
What is the significance of simple diffusion for cells?
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What will happen to a cell when it is placed in a hypertonic solution?
What will happen to a cell when it is placed in a hypertonic solution?
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Facilitated diffusion is a form of:
Facilitated diffusion is a form of:
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Which transport process is used by cells to move glucose across the plasma membrane?
Which transport process is used by cells to move glucose across the plasma membrane?
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What is the primary difference between facilitated diffusion and active transport?
What is the primary difference between facilitated diffusion and active transport?
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Which of the following statements is TRUE regarding the behavior of a cell in a hypotonic solution?
Which of the following statements is TRUE regarding the behavior of a cell in a hypotonic solution?
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Which type of membrane transport is primarily responsible for moving large molecules and multimolecular particles?
Which type of membrane transport is primarily responsible for moving large molecules and multimolecular particles?
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What is a shared characteristic between both active and facilitated membrane transport?
What is a shared characteristic between both active and facilitated membrane transport?
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In facilitated diffusion, how does the carrier protein facilitate the movement of solute across the membrane?
In facilitated diffusion, how does the carrier protein facilitate the movement of solute across the membrane?
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Which statement accurately describes the function of carrier proteins in membrane transport?
Which statement accurately describes the function of carrier proteins in membrane transport?
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A substance is moving across a cell membrane from an area of high concentration to an area of low concentration, with the help of a transport protein. What type of transport is most likely occurring?
A substance is moving across a cell membrane from an area of high concentration to an area of low concentration, with the help of a transport protein. What type of transport is most likely occurring?
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What is a key difference between channel proteins and carrier proteins in membrane transport?
What is a key difference between channel proteins and carrier proteins in membrane transport?
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The 'transport maximum' (Tm) in carrier-mediated transport is a direct result of which of the following?
The 'transport maximum' (Tm) in carrier-mediated transport is a direct result of which of the following?
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A cell membrane is said to exhibit 'specificity' in carrier-mediated transport. What does this imply?
A cell membrane is said to exhibit 'specificity' in carrier-mediated transport. What does this imply?
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Which of the following is NOT a characteristic of facilitated diffusion?
Which of the following is NOT a characteristic of facilitated diffusion?
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What is the primary difference between transport via carriers compared with transport via channels?
What is the primary difference between transport via carriers compared with transport via channels?
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The phenomenon where several related molecules compete to bind to the same transporter protein is known as what?
The phenomenon where several related molecules compete to bind to the same transporter protein is known as what?
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What is the primary mechanism by which protein hormones are secreted from cells?
What is the primary mechanism by which protein hormones are secreted from cells?
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What is the fundamental characteristic of a cell's membrane when a membrane potential exists?
What is the fundamental characteristic of a cell's membrane when a membrane potential exists?
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If a membrane has a higher potential, what does that indicate about the charge separation across the membrane?
If a membrane has a higher potential, what does that indicate about the charge separation across the membrane?
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What is the direct impact of the Na+-K+ pump on the membrane potential?
What is the direct impact of the Na+-K+ pump on the membrane potential?
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What is the main mechanism behind most of the resting membrane potential?
What is the main mechanism behind most of the resting membrane potential?
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What does the negative sign of the resting membrane potential indicate?
What does the negative sign of the resting membrane potential indicate?
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Which of the following ions directly contributes to the resting membrane potential?
Which of the following ions directly contributes to the resting membrane potential?
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In addition to the Na+/K+ pump, how do Na+ and K+ ions cross the plasma membrane?
In addition to the Na+/K+ pump, how do Na+ and K+ ions cross the plasma membrane?
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What primarily maintains the concentration gradient of Na+ and K+ that is crucial for the resting membrane potential?
What primarily maintains the concentration gradient of Na+ and K+ that is crucial for the resting membrane potential?
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A cell has a resting membrane potential of -70 mV. How would you describe the charge inside the cell relative to the outside?
A cell has a resting membrane potential of -70 mV. How would you describe the charge inside the cell relative to the outside?
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Flashcards
What is the function of the plasma membrane?
What is the function of the plasma membrane?
The plasma membrane provides a physical barrier for cells, controlling what enters and leaves.
What is the lipid bilayer?
What is the lipid bilayer?
The lipid bilayer forms the foundation of the plasma membrane, acting as a selective barrier.
What are the components of the plasma membrane?
What are the components of the plasma membrane?
The plasma membrane is composed of a lipid bilayer, proteins, and carbohydrates, each contributing to its function.
What is the role of proteins in the plasma membrane?
What is the role of proteins in the plasma membrane?
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How are cells held together?
How are cells held together?
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What are tight junctions?
What are tight junctions?
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What are gap junctions?
What are gap junctions?
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What is fibronectin?
What is fibronectin?
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What is collagen?
What is collagen?
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What is elastin?
What is elastin?
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What is the extracellular matrix?
What is the extracellular matrix?
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What is passive transport?
What is passive transport?
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What is active transport?
What is active transport?
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Unassisted Membrane Transport
Unassisted Membrane Transport
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Diffusion
Diffusion
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Concentration Gradient
Concentration Gradient
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Electrical Gradient
Electrical Gradient
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Lipid Solubility
Lipid Solubility
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Active Transport
Active Transport
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Which substances can dissolve through the plasma membrane?
Which substances can dissolve through the plasma membrane?
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Which substances need assisted transport?
Which substances need assisted transport?
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Passive transport
Passive transport
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Osmosis
Osmosis
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Isotonic solution
Isotonic solution
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Hypotonic solution
Hypotonic solution
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Hypertonic solution
Hypertonic solution
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Carrier-mediated transport
Carrier-mediated transport
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Facilitated diffusion
Facilitated diffusion
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What is facilitated diffusion?
What is facilitated diffusion?
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What is a carrier protein?
What is a carrier protein?
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What is a channel protein?
What is a channel protein?
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What is Transport Maximum (Tm)?
What is Transport Maximum (Tm)?
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What is Competition in carrier-mediated transport?
What is Competition in carrier-mediated transport?
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What is Saturation in carrier-mediated transport?
What is Saturation in carrier-mediated transport?
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Exocytosis - What is it?
Exocytosis - What is it?
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Membrane Potential - What is it?
Membrane Potential - What is it?
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Na+/K+ Pump - Function?
Na+/K+ Pump - Function?
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K+ Leak Channels - Role in membrane potential?
K+ Leak Channels - Role in membrane potential?
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Resting Membrane Potential - What is it?
Resting Membrane Potential - What is it?
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Ions involved in RMP - Which ones?
Ions involved in RMP - Which ones?
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Na+/K+ Pump - Importance for RMP?
Na+/K+ Pump - Importance for RMP?
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Passive Transport - What is it?
Passive Transport - What is it?
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Active Transport - What is it?
Active Transport - What is it?
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Vesicular Transport - What is it?
Vesicular Transport - What is it?
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Study Notes
Introduction to Pathophysiology (MPLC031)
- Course instructor: Dr. N.S. Mapfumari
- Email: [email protected]
- Phone: 012 521.5692
- Office: N407 BMS
- Institution: Sefako Makgatho Health Sciences University
Excitable Tissues
- Excitable tissues are a topic related to the subject of the lecture
Plasma Membrane
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The plasma membrane is a critical component of cells, forming a boundary around every cell.
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It controls movement of substances between the intracellular and extracellular fluids (ICF and ECF).
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It maintains differences in ion concentrations inside and outside the cell, impacting membrane electrical activity.
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It participates in joining cells to form tissues and organs.
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It enables cells to respond to changes in the environment or receive signals from other cells (cell-to-cell communication).
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The membrane is composed primarily of a phospholipid bilayer, studded with various proteins (integral and peripheral).
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Phospholipid Molecule:
- Composed of a polar head (hydrophilic) and nonpolar fatty acid tails (hydrophobic).
- The hydrophilic heads interact with the aqueous environments (ICF and ECF).
- The hydrophobic tails are oriented inward, forming a barrier to water-soluble substances.
Lecture I - Expected Outcomes
- List the functions of the plasma membrane.
- Describe the organization of the lipid bilayer.
- Describe the structure of the plasma membrane.
- List the components of the plasma membrane.
- Discuss the function of the components of the plasma membrane.
- Describe cell-to-cell adhesion.
- Identify ways cells are held together.
Functions of the Plasma Membrane
- Forms a boundary for every cell, acting as a mechanical barrier.
- Controls the movement of molecules between the ICF and ECF (semi-permeable).
- Maintains differences in ion concentrations inside and outside the cell, crucial for membrane electrical activity.
- Participates in joining cells to form tissues and organs.
- Plays a role in the ability of a cell to respond to environmental changes or signals (intercellular communication).
Components of the Plasma Membrane
- Phospholipids: forms the bilayer.
- Cholesterol: contributes to membrane stability and fluidity .
- Integral proteins: extends through the membrane, act as channels, carriers, receptors.
- Peripheral proteins: do not penetrate the membrane, found on the inner or outer surface, act as enzymes or structural components.
- Carbohydrates: found on the outer surface, often bound to proteins or lipids (glycoproteins/glycolipids), serve as cell markers and recognition sites for other cells.
Functions of Phospholipid Bilayer
- Forms the basic structure of the plasma membrane.
- Acts as the primary barrier for diffusion of water-soluble substances.
- Maintains different concentrations of dissolved substances within ICF and ECF.
- Provides the fluidity of the membrane.
Organization of Phospholipids
- Phospholipids organize into a bilayer in water, with hydrophilic heads facing outward and hydrophobic tails facing inward.
- This arrangement creates a barrier to water-soluble substances.
Intracellular Fluid (ICF)
- The fluid inside the cell
Extracellular Fluid (ECF)
- The fluid outside the cell
Functions of Cholesterol
- Cholesterol contributes to the stability and fluidity of the membrane.
Types of Membrane Proteins
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Integral proteins: extend through the entire membrane (transmembrane proteins)
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Peripheral proteins: associated with the inner or outer surface of the membrane.
Functions of Membrane Proteins
- Channels: Facilitate passage of small ions (e.g. Na+, K+).
- Leak channels: allows unregulated passage.
- Gated channels: passage controlled by internal or external stimuli.
- Carriers: facilitate passage across the membrane of substances that do not readily pass (e.g., glucose, proteins)..
- Docking Marker acceptors for secretory vesicles via stimulation.
- Membrane-bound enzymes (control specific chemical reactions)
- Receptors (respond to chemical messengers, water-soluble hormones).
- Cell adhesion molecules (CAMs)that hold cells together.
- Self-identity markers (in conjunction with carbs).
Characteristics & Functions of Membrane Carbohydrates
- Short carbohydrate chains on the outer membrane surface.
- Bound to proteins (glycoproteins) and lipids (glycolipids).
- Membrane carbohydrates serve as self-recognition markers.
- Allows the joining and forming of tissues.
- Involved in tissue growth.
Function of Membrane Proteins
- Serve as channels
- Serve as carriers
- Serve as receptor sites
- Serve as docking marker acceptors
- Determine the fluidity of the membrane
Functions Of Lipid Bilayer
- Serves as a structural basement
- Serves as primary barrier to diffusion of water soluble solutions between ICF & ECF
Cell-to-cell Adhesion
- Similar cells organize into appropriate groupings by means of carbohydrate markers on the membrane surface.
- Arranged cells are held together in three ways:
- Extracellular matrix.
- CAMs (membrane proteins).
- Specialized cell junctions.
Extracellular Matrix
- Acts like biological glue.
- Binds cells together.
- Composed of collagen, elastin, and fibronectin.
- Collagen forms flexible non-elastic fibers or sheets providing tensile strength.
- Elastin allows tissues to stretch and recoil.
- Fibronectin promotes cell adhesion and holds cells in position.
Specialized Cell Junctions
- Tight junctions: Prevent molecules from passing through the intercellular space.
- Desmosomes: Bind adjacent cells together like molecular velcro, helping form a tension-reducing network of fibers.
- Gap junctions: Allow ions and small molecules to pass for intercellular communication.
Membrane Transport
- Materials can pass between the ECF and ICF by unassisted (e.g., O2, CO2, fatty acids) and assisted (e.g. ions such as Na+, K+, glucose, proteins - facilitated transport, carrier-mediated transport, and vesicular transport) mechanisms.
- Transport mechanisms may be passive (e.g., simple diffusion, osmosis) or active (e.g., primary active transport, secondary active transport).
Particle Solubility and Size
- Lipid solubility and particle size influence whether particles can permeate the cell membrane without assistance.
- Highly lipid-soluble particles dissolve in the lipid bilayer, readily permeating the membrane. Uncharged or nonpolar molecules are highly lipid-soluble.
- Non-lipid soluble particles (e.g., ions + polar molecules like glucose and proteins) require assisted transport.
Simple Diffusion
- Unassisted, passive movement of molecules from high to low concentration.
- Occurs until equilibrium is reached.
- Molecules can permeate the lipid bilayer or channel proteins depending on permeability.
- Factors influencing the rate of simple diffusion:
- Concentration gradient magnitude.
- Membrane permeability of the substance.
- Surface area of the membrane.
- Molecular weight of the substance.
- Distance through which diffusion takes place
Electrochemical Gradient
- The combined effect of concentration and electrical gradients that influence the movement of ions across the cell membrane .
- Charged molecules always follow both the concentration gradient and electrical gradient together. This combination of forces is termed Electrochemical Gradient.
Water Transport
- Water passes through the membrane through gaps between the phospholipid tails and through specialized protein channels(aquaporins)..
- Net diffusion of water down its concentration gradient is osmosis.
Tonicity
- Isotonic solution has the same concentration of nonpenetrating solutes as the cell.
- Hypotonic solution has a lower concentration of nonpenetrating solutes than the cell.
- Hypertonic solution has a higher concentration of nonpenetrating solutes than the cell.
Assisted Membrane Transport
- Designed for poorly lipid-soluble, large molecules (e.g., glucose, proteins, amino acids).
- Two types:
- Carrier-mediated transport.
- Vesicular transport.
Carrier-mediated Transport
- Two types:
- Facilitated diffusion (moves substances down concentration and electrochemical gradients).
- Active transport (moves substances against concentration and electrochemical gradients - requires energy).
Facilitated Diffusion
- Small particles are transported by membrane carrier proteins.
- Carrier changes conformation to expose binding sites alternately to either side of the membrane.
- The process is passive, moving substances down the concentration gradient.
Active Transport
- Two types:
- Primary active transport: directly uses ATP energy to move a substance against its concentration gradient. (e.g., Na+/K+ pump).
- Secondary active transport: couples the movement of one substance down its concentration gradient to the movement of another substance against its concentration gradient.
Vesicular Transport
- Active process where materials are moved into or out of the cell wrapped in a membrane vesicle.
- Two types:
- Endocytosis.
- Exocytosis.
Endocytosis
- Three types:
- Pinocytosis (nonselective uptake of extracellular fluid - ECF).
- Receptor-mediated endocytosis (selective uptake of large molecules).
- Phagocytosis (selective uptake of multimolecular particles).
Exocytosis
- Reverse of endocytosis through which substances are secreted from the cell (e.g., protein hormones, enzymes).
Membrane Potential
- All cells have a membrane potential (electrical polarization).
- It's the separation of opposite charges across the membrane (difference in relative number of cations and anions in the ICF and ECF).
- Resting membrane potential is -70 mV (inside the cell is negative relative to the outside)..
- Primarily determined by the different permeability of the membrane to different ions.
Role of the Na+-K+ Pump
- Transports 3 Na+ ions out of the cell for every 2 K+ ions pumped into the cell.
- Results in a net loss of positive charge from inside the cell maintaining unequal distribution across the membrane.
- Plays a crucial indirect role in establishing the resting membrane potential.
Resting Membrane Potential
- A constant membrane potential in non-excitable cells at rest.
- Typically, resting membrane potential is -70mV.
- Due to unequal permeability of the cell membrane to potassium, Sodium, and other ions.
- Maintained by the Na+/K+ pump and the permeability differences to various ions.
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Description
This quiz explores key functions of the plasma membrane and its role in cellular integrity. Questions focus on permeability, cellular boundaries, and the extracellular matrix. Test your knowledge on how the plasma membrane contributes to maintaining essential cellular functions.