Cell Biology: Plasma Membrane Functions
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Questions and Answers

Which of the following is NOT a primary function of the plasma membrane?

  • Regulating the movement of substances between the intracellular and extracellular fluids.
  • Establishing a mechanical barrier for the cell.
  • Generating energy for cellular functions. (correct)
  • Maintaining ion concentration differences across the membrane.
  • The plasma membrane is described as 'semi-permeable,' what does this term most accurately describe?

  • The membrane allows all substances to pass freely across it.
  • The membrane restricts movement of all molecules at all times.
  • The membrane only allows for the exit of secretory and waste products, and no other molecules.
  • The membrane selectively allows some substances to cross while restricting others. (correct)
  • What is the role of the plasma membrane in maintaining ion concentration differences?

  • It facilitates the free movement of ions to reach equilibrium.
  • It uses active transport to equalize ion concentrations on both sides.
  • It prevents movement of all ions to maintain a static environment.
  • It employs selective permeability and ion pumps to create concentration gradients. (correct)
  • In the context of cellular function, what is the significance of the plasma membrane forming the boundary of the cell?

    <p>It provides a physical barrier that contains cellular contents, and creates a specific micro-environment. (B)</p> Signup and view all the answers

    Which statement best describes the relationship between the plasma membrane and the intracellular fluid (ICF) and extracellular fluid (ECF)?

    <p>The plasma membrane regulates movement of substances, controlling the composition of both ICF and ECF. (A)</p> Signup and view all the answers

    What is the primary role of carbohydrate markers on the cell membrane?

    <p>To enable cells to organize into appropriate groupings. (A)</p> Signup and view all the answers

    Which component of the extracellular matrix is predominantly responsible for providing tensile strength to tissues?

    <p>Collagen (C)</p> Signup and view all the answers

    What is the main function of elastin within the extracellular matrix?

    <p>To allow tissues to stretch and then recoil. (A)</p> Signup and view all the answers

    Which type of cell junction best describes structures that prevent molecules from passing through the intercellular space?

    <p>Tight junctions (D)</p> Signup and view all the answers

    Which of the following best describes the permeability of the plasma membrane?

    <p>It is selectively permeable, allowing some substances to pass while preventing others. (B)</p> Signup and view all the answers

    Which of these substances can pass directly through the lipid bilayer of the plasma membrane?

    <p>Oxygen (B)</p> Signup and view all the answers

    Which of the following is NOT a transport mechanism across the plasma membrane?

    <p>Granulation (C)</p> Signup and view all the answers

    What is the function of fibronectin in the extracellular matrix?

    <p>To facilitate cell adhesion and position. (C)</p> Signup and view all the answers

    Which of the following best describes a substance that is considered impermeable to a cell membrane?

    <p>A substance that cannot cross the membrane by any means. (B)</p> Signup and view all the answers

    What are the two primary properties that determine if a substance can permeate a cell membrane without assistance?

    <p>Lipid solubility and particle size. (A)</p> Signup and view all the answers

    A substance that can pass through the membrane via simple diffusion must be:

    <p>Nonpolar and lipid soluble. (B)</p> Signup and view all the answers

    Assisted transport is required for which types of substances to cross a cell membrane?

    <p>Charged particles like ions and polar molecules. (B)</p> Signup and view all the answers

    Which of the following is an example of a passive force that drives unassisted transport across a cell membrane?

    <p>Diffusion down a concentration gradient. (C)</p> Signup and view all the answers

    Which of the following is primarily transported across a cell via unassisted means?

    <p>Oxygen (B)</p> Signup and view all the answers

    What distinguishes active transport from passive transport across a cell membrane?

    <p>Active transport requires cellular energy, while passive does not. (B)</p> Signup and view all the answers

    What is the significance of simple diffusion for cells?

    <p>It is a critical passive process that all cells use for survival. (D)</p> Signup and view all the answers

    What will happen to a cell when it is placed in a hypertonic solution?

    <p>The cell will shrink as water diffuses out of it. (D)</p> Signup and view all the answers

    Facilitated diffusion is a form of:

    <p>Carrier-mediated transport that does not require cellular energy. (C)</p> Signup and view all the answers

    Which transport process is used by cells to move glucose across the plasma membrane?

    <p>Facilitated diffusion using carrier proteins. (C)</p> Signup and view all the answers

    What is the primary difference between facilitated diffusion and active transport?

    <p>Facilitated diffusion does not require energy; active transport requires cellular energy. (A)</p> Signup and view all the answers

    Which of the following statements is TRUE regarding the behavior of a cell in a hypotonic solution?

    <p>Water diffuses into the cell, causing it to swell. (B)</p> Signup and view all the answers

    Which type of membrane transport is primarily responsible for moving large molecules and multimolecular particles?

    <p>Vesicular transport. (D)</p> Signup and view all the answers

    What is a shared characteristic between both active and facilitated membrane transport?

    <p>Both require carrier proteins as part of the process. (A)</p> Signup and view all the answers

    In facilitated diffusion, how does the carrier protein facilitate the movement of solute across the membrane?

    <p>By changing its shape to expose binding sites to both sides of the membrane. (A)</p> Signup and view all the answers

    Which statement accurately describes the function of carrier proteins in membrane transport?

    <p>They facilitate the movement of water-soluble substances across the membrane through conformational changes. (D)</p> Signup and view all the answers

    A substance is moving across a cell membrane from an area of high concentration to an area of low concentration, with the help of a transport protein. What type of transport is most likely occurring?

    <p>Facilitated diffusion (A)</p> Signup and view all the answers

    What is a key difference between channel proteins and carrier proteins in membrane transport?

    <p>Channel proteins are always open, while carrier proteins must change conformation. (D)</p> Signup and view all the answers

    The 'transport maximum' (Tm) in carrier-mediated transport is a direct result of which of the following?

    <p>The limited number of carrier proteins available in the membrane. (D)</p> Signup and view all the answers

    A cell membrane is said to exhibit 'specificity' in carrier-mediated transport. What does this imply?

    <p>Each carrier protein will only transport specific substances or a few closely related compounds. (D)</p> Signup and view all the answers

    Which of the following is NOT a characteristic of facilitated diffusion?

    <p>It requires direct input of energy in the form of ATP. (D)</p> Signup and view all the answers

    What is the primary difference between transport via carriers compared with transport via channels?

    <p>Carriers need to undergo a conformational change, but channels do not. (B)</p> Signup and view all the answers

    The phenomenon where several related molecules compete to bind to the same transporter protein is known as what?

    <p>Competition (C)</p> Signup and view all the answers

    What is the primary mechanism by which protein hormones are secreted from cells?

    <p>Endocytic vesicle fusion with the plasma membrane (B)</p> Signup and view all the answers

    What is the fundamental characteristic of a cell's membrane when a membrane potential exists?

    <p>A separation of opposite charges across the membrane. (C)</p> Signup and view all the answers

    If a membrane has a higher potential, what does that indicate about the charge separation across the membrane?

    <p>The charge separation is greater. (C)</p> Signup and view all the answers

    What is the direct impact of the Na+-K+ pump on the membrane potential?

    <p>It makes a small direct contribution to the resting membrane potential. (A)</p> Signup and view all the answers

    What is the main mechanism behind most of the resting membrane potential?

    <p>Passive diffusion of K+ ions (D)</p> Signup and view all the answers

    What does the negative sign of the resting membrane potential indicate?

    <p>The intracellular space is negatively charged compared to the outside. (B)</p> Signup and view all the answers

    Which of the following ions directly contributes to the resting membrane potential?

    <p>K+ (C)</p> Signup and view all the answers

    In addition to the Na+/K+ pump, how do Na+ and K+ ions cross the plasma membrane?

    <p>Through protein channels specific to each ion (C)</p> Signup and view all the answers

    What primarily maintains the concentration gradient of Na+ and K+ that is crucial for the resting membrane potential?

    <p>The activity of the Na+-K+ pump (D)</p> Signup and view all the answers

    A cell has a resting membrane potential of -70 mV. How would you describe the charge inside the cell relative to the outside?

    <p>The inside of the cell is more negative than the outside. (D)</p> Signup and view all the answers

    Flashcards

    What is the function of the plasma membrane?

    The plasma membrane provides a physical barrier for cells, controlling what enters and leaves.

    What is the lipid bilayer?

    The lipid bilayer forms the foundation of the plasma membrane, acting as a selective barrier.

    What are the components of the plasma membrane?

    The plasma membrane is composed of a lipid bilayer, proteins, and carbohydrates, each contributing to its function.

    What is the role of proteins in the plasma membrane?

    Proteins embedded in the plasma membrane facilitate transport, communication, and structural support.

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    How are cells held together?

    Cells can adhere to each other through structures like tight junctions, desmosomes, and gap junctions.

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    What are tight junctions?

    A type of cell junction that acts like molecular Velcro, binding adjacent cells together and preventing leakage between them.

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    What are gap junctions?

    A specialized cell junction that forms channels between cells, allowing ions and small molecules to pass for intercellular communication.

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    What is fibronectin?

    A substance that promotes cell adhesion, holding cells in position within the extracellular matrix.

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    What is collagen?

    A type of protein fiber in the extracellular matrix that provides tensile strength, making tissues flexible and resistant to stretching.

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    What is elastin?

    A type of protein fiber in the extracellular matrix that allows tissues to stretch and then recoil, providing elasticity.

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    What is the extracellular matrix?

    The biological glue that binds cells together, consisting of interconnected protein fibers and ground substance.

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    What is passive transport?

    The movement of substances across the cell membrane without requiring energy, driven by concentration gradients or pressure differences.

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    What is active transport?

    The movement of substances across the cell membrane that requires energy, going against concentration gradients.

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    Unassisted Membrane Transport

    Materials move across the cell membrane without assistance from the cell. It's a passive process where substances follow natural gradients.

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    Diffusion

    The movement of a substance across a membrane from a region of higher concentration to a region of lower concentration. This is a passive process driven by the concentration difference.

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    Concentration Gradient

    The difference in concentration of a substance between two areas. It's the driving force for diffusion. The steeper the gradient, the faster diffusion occurs.

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    Electrical Gradient

    When a substance crosses the cell membrane due to the differences in electrical charge on either side of the membrane. It's a passive process.

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    Lipid Solubility

    It's the tendency of a substance to dissolve in a lipid bilayer. Lipid soluble substances can easily pass through the cell membrane.

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    Active Transport

    The cell spends energy to move substances across the membrane, going against their natural concentration or electrical gradient.

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    Which substances can dissolve through the plasma membrane?

    Substances can pass through the membrane in passive transport. These substances are highly soluble in lipids.

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    Which substances need assisted transport?

    Substances like Na+ ions, glucose, and proteins cannot dissolve in lipids and need assistance to cross the membrane.

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    Passive transport

    Movement of substances across cell membranes without the use of cellular energy. Examples include diffusion, osmosis, and filtration.

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    Osmosis

    Movement of water across a semipermeable membrane from an area of high water concentration to an area of low water concentration.

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    Isotonic solution

    A solution that has the same concentration of solutes as the cell's cytoplasm. Water moves equally in and out of the cell, maintaining its volume.

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    Hypotonic solution

    A solution that has a lower concentration of solutes than the cell's cytoplasm. Water moves into the cell, causing it to swell.

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    Hypertonic solution

    A solution that has a higher concentration of solutes than the cell's cytoplasm. Water moves out of the cell, causing it to shrink.

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    Carrier-mediated transport

    A type of membrane transport that uses carrier proteins to move molecules across the membrane. It can be either facilitated diffusion or active transport.

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    Facilitated diffusion

    A form of carrier-mediated transport that moves molecules across the membrane down their concentration gradient. It does not require energy.

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    What is facilitated diffusion?

    A type of passive transport where a substance moves across a membrane with the help of a protein carrier, but still following the concentration gradient, without requiring energy.

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    What is a carrier protein?

    The specific protein involved in facilitated diffusion that binds to a substance and helps its movement across the membrane, changing its conformation during the process.

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    What is a channel protein?

    A type of membrane transport protein that allows specific substances to move across the membrane via a continuous passage, unlike carrier proteins.

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    What is Transport Maximum (Tm)?

    The maximum rate at which a substance can be transported by a carrier protein, determined by the number of available binding sites on the carrier.

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    What is Competition in carrier-mediated transport?

    The phenomenon where multiple substances compete for binding to the same carrier protein, potentially slowing down the transport of any one of them.

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    What is Saturation in carrier-mediated transport?

    The condition where all available binding sites on a carrier protein are occupied by the transported substance, resulting in a plateau in the transport rate.

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    Exocytosis - What is it?

    The process of releasing cellular contents, like hormones or enzymes, through fusion of a membrane-bound vesicle with the cell's outer membrane.

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    Membrane Potential - What is it?

    The difference in electrical charge across a cell's membrane, caused by an uneven distribution of ions.

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    Na+/K+ Pump - Function?

    The Na+/K+ pump moves three sodium ions (Na+) out of the cell for every two potassium ions (K+) it pumps in. This creates a negative charge inside the cell, contributing to the membrane potential.

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    K+ Leak Channels - Role in membrane potential?

    K+ leak channels allow a small amount of potassium ions (K+) to leak out of the cell, further increasing the negative charge inside. This contributes to the resting membrane potential.

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    Resting Membrane Potential - What is it?

    The stable, negative membrane potential of a cell when it's not actively signaling or transmitting impulses.

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    Ions involved in RMP - Which ones?

    Sodium (Na+), potassium (K+), and large, negatively charged intracellular proteins are the main players in generating the resting membrane potential (RMP).

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    Na+/K+ Pump - Importance for RMP?

    The Na+/K+ pump actively maintains the concentration gradients of sodium and potassium ions across the membrane, which is essential for the resting membrane potential.

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    Passive Transport - What is it?

    The movement of substances across a cell membrane without requiring energy from the cell, driven by a concentration gradient or pressure difference.

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    Active Transport - What is it?

    The movement of substances across a cell membrane that requires energy from the cell, typically going against a concentration gradient.

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    Vesicular Transport - What is it?

    A method of transporting substances across the cell membrane using membrane-bound sacs called vesicles. Examples include exocytosis and endocytosis.

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    Study Notes

    Introduction to Pathophysiology (MPLC031)

    • Course instructor: Dr. N.S. Mapfumari
    • Email: [email protected]
    • Phone: 012 521.5692
    • Office: N407 BMS
    • Institution: Sefako Makgatho Health Sciences University

    Excitable Tissues

    • Excitable tissues are a topic related to the subject of the lecture

    Plasma Membrane

    • The plasma membrane is a critical component of cells, forming a boundary around every cell.

    • It controls movement of substances between the intracellular and extracellular fluids (ICF and ECF).

    • It maintains differences in ion concentrations inside and outside the cell, impacting membrane electrical activity.

    • It participates in joining cells to form tissues and organs.

    • It enables cells to respond to changes in the environment or receive signals from other cells (cell-to-cell communication).

    • The membrane is composed primarily of a phospholipid bilayer, studded with various proteins (integral and peripheral).

    • Phospholipid Molecule:

      • Composed of a polar head (hydrophilic) and nonpolar fatty acid tails (hydrophobic).
      • The hydrophilic heads interact with the aqueous environments (ICF and ECF).
      • The hydrophobic tails are oriented inward, forming a barrier to water-soluble substances.

    Lecture I - Expected Outcomes

    • List the functions of the plasma membrane.
    • Describe the organization of the lipid bilayer.
    • Describe the structure of the plasma membrane.
    • List the components of the plasma membrane.
    • Discuss the function of the components of the plasma membrane.
    • Describe cell-to-cell adhesion.
    • Identify ways cells are held together.

    Functions of the Plasma Membrane

    • Forms a boundary for every cell, acting as a mechanical barrier.
    • Controls the movement of molecules between the ICF and ECF (semi-permeable).
    • Maintains differences in ion concentrations inside and outside the cell, crucial for membrane electrical activity.
    • Participates in joining cells to form tissues and organs.
    • Plays a role in the ability of a cell to respond to environmental changes or signals (intercellular communication).

    Components of the Plasma Membrane

    • Phospholipids: forms the bilayer.
    • Cholesterol: contributes to membrane stability and fluidity .
    • Integral proteins: extends through the membrane, act as channels, carriers, receptors.
    • Peripheral proteins: do not penetrate the membrane, found on the inner or outer surface, act as enzymes or structural components.
    • Carbohydrates: found on the outer surface, often bound to proteins or lipids (glycoproteins/glycolipids), serve as cell markers and recognition sites for other cells.

    Functions of Phospholipid Bilayer

    • Forms the basic structure of the plasma membrane.
    • Acts as the primary barrier for diffusion of water-soluble substances.
    • Maintains different concentrations of dissolved substances within ICF and ECF.
    • Provides the fluidity of the membrane.

    Organization of Phospholipids

    • Phospholipids organize into a bilayer in water, with hydrophilic heads facing outward and hydrophobic tails facing inward.
    • This arrangement creates a barrier to water-soluble substances.

    Intracellular Fluid (ICF)

    • The fluid inside the cell

    Extracellular Fluid (ECF)

    • The fluid outside the cell

    Functions of Cholesterol

    • Cholesterol contributes to the stability and fluidity of the membrane.

    Types of Membrane Proteins

    • Integral proteins: extend through the entire membrane (transmembrane proteins)

    • Peripheral proteins: associated with the inner or outer surface of the membrane.

    Functions of Membrane Proteins

    • Channels: Facilitate passage of small ions (e.g. Na+, K+).
      • Leak channels: allows unregulated passage.
      • Gated channels: passage controlled by internal or external stimuli.
    • Carriers: facilitate passage across the membrane of substances that do not readily pass (e.g., glucose, proteins)..
    • Docking Marker acceptors for secretory vesicles via stimulation.
    • Membrane-bound enzymes (control specific chemical reactions)
    • Receptors (respond to chemical messengers, water-soluble hormones).
    • Cell adhesion molecules (CAMs)that hold cells together.
    • Self-identity markers (in conjunction with carbs).

    Characteristics & Functions of Membrane Carbohydrates

    • Short carbohydrate chains on the outer membrane surface.
    • Bound to proteins (glycoproteins) and lipids (glycolipids).
    • Membrane carbohydrates serve as self-recognition markers.
    • Allows the joining and forming of tissues.
    • Involved in tissue growth.

    Function of Membrane Proteins

    • Serve as channels
    • Serve as carriers
    • Serve as receptor sites
    • Serve as docking marker acceptors
    • Determine the fluidity of the membrane

    Functions Of Lipid Bilayer

    • Serves as a structural basement
    • Serves as primary barrier to diffusion of water soluble solutions between ICF & ECF

    Cell-to-cell Adhesion

    • Similar cells organize into appropriate groupings by means of carbohydrate markers on the membrane surface.
    • Arranged cells are held together in three ways:
      • Extracellular matrix.
      • CAMs (membrane proteins).
      • Specialized cell junctions.

    Extracellular Matrix

    • Acts like biological glue.
    • Binds cells together.
    • Composed of collagen, elastin, and fibronectin.
      • Collagen forms flexible non-elastic fibers or sheets providing tensile strength.
      • Elastin allows tissues to stretch and recoil.
      • Fibronectin promotes cell adhesion and holds cells in position.

    Specialized Cell Junctions

    • Tight junctions: Prevent molecules from passing through the intercellular space.
    • Desmosomes: Bind adjacent cells together like molecular velcro, helping form a tension-reducing network of fibers.
    • Gap junctions: Allow ions and small molecules to pass for intercellular communication.

    Membrane Transport

    • Materials can pass between the ECF and ICF by unassisted (e.g., O2, CO2, fatty acids) and assisted (e.g. ions such as Na+, K+, glucose, proteins - facilitated transport, carrier-mediated transport, and vesicular transport) mechanisms.
    • Transport mechanisms may be passive (e.g., simple diffusion, osmosis) or active (e.g., primary active transport, secondary active transport).

    Particle Solubility and Size

    • Lipid solubility and particle size influence whether particles can permeate the cell membrane without assistance.
      • Highly lipid-soluble particles dissolve in the lipid bilayer, readily permeating the membrane. Uncharged or nonpolar molecules are highly lipid-soluble.
      • Non-lipid soluble particles (e.g., ions + polar molecules like glucose and proteins) require assisted transport.

    Simple Diffusion

    • Unassisted, passive movement of molecules from high to low concentration.
    • Occurs until equilibrium is reached.
    • Molecules can permeate the lipid bilayer or channel proteins depending on permeability.
    • Factors influencing the rate of simple diffusion:
      • Concentration gradient magnitude.
      • Membrane permeability of the substance.
      • Surface area of the membrane.
      • Molecular weight of the substance.
      • Distance through which diffusion takes place

    Electrochemical Gradient

    • The combined effect of concentration and electrical gradients that influence the movement of ions across the cell membrane .
    • Charged molecules always follow both the concentration gradient and electrical gradient together. This combination of forces is termed Electrochemical Gradient.

    Water Transport

    • Water passes through the membrane through gaps between the phospholipid tails and through specialized protein channels(aquaporins)..
    • Net diffusion of water down its concentration gradient is osmosis.

    Tonicity

    • Isotonic solution has the same concentration of nonpenetrating solutes as the cell.
    • Hypotonic solution has a lower concentration of nonpenetrating solutes than the cell.
    • Hypertonic solution has a higher concentration of nonpenetrating solutes than the cell.

    Assisted Membrane Transport

    • Designed for poorly lipid-soluble, large molecules (e.g., glucose, proteins, amino acids).
    • Two types:
      • Carrier-mediated transport.
      • Vesicular transport.

    Carrier-mediated Transport

    • Two types:
      • Facilitated diffusion (moves substances down concentration and electrochemical gradients).
      • Active transport (moves substances against concentration and electrochemical gradients - requires energy).

    Facilitated Diffusion

    • Small particles are transported by membrane carrier proteins.
    • Carrier changes conformation to expose binding sites alternately to either side of the membrane.
    • The process is passive, moving substances down the concentration gradient.

    Active Transport

    • Two types:
      • Primary active transport: directly uses ATP energy to move a substance against its concentration gradient. (e.g., Na+/K+ pump).
      • Secondary active transport: couples the movement of one substance down its concentration gradient to the movement of another substance against its concentration gradient.

    Vesicular Transport

    • Active process where materials are moved into or out of the cell wrapped in a membrane vesicle.
    • Two types:
      • Endocytosis.
      • Exocytosis.

    Endocytosis

    • Three types:
      • Pinocytosis (nonselective uptake of extracellular fluid - ECF).
      • Receptor-mediated endocytosis (selective uptake of large molecules).
      • Phagocytosis (selective uptake of multimolecular particles).

    Exocytosis

    • Reverse of endocytosis through which substances are secreted from the cell (e.g., protein hormones, enzymes).

    Membrane Potential

    • All cells have a membrane potential (electrical polarization).
    • It's the separation of opposite charges across the membrane (difference in relative number of cations and anions in the ICF and ECF).
    • Resting membrane potential is -70 mV (inside the cell is negative relative to the outside)..
    • Primarily determined by the different permeability of the membrane to different ions.

    Role of the Na+-K+ Pump

    • Transports 3 Na+ ions out of the cell for every 2 K+ ions pumped into the cell.
    • Results in a net loss of positive charge from inside the cell maintaining unequal distribution across the membrane.
    • Plays a crucial indirect role in establishing the resting membrane potential.

    Resting Membrane Potential

    • A constant membrane potential in non-excitable cells at rest.
    • Typically, resting membrane potential is -70mV.
    • Due to unequal permeability of the cell membrane to potassium, Sodium, and other ions.
    • Maintained by the Na+/K+ pump and the permeability differences to various ions.

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    This quiz explores key functions of the plasma membrane and its role in cellular integrity. Questions focus on permeability, cellular boundaries, and the extracellular matrix. Test your knowledge on how the plasma membrane contributes to maintaining essential cellular functions.

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