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Questions and Answers
What is the function of a microtome in histology?
What is the function of a microtome in histology?
- Enhancing the resolution of microscopic images
- Staining tissues with different dyes
- Sectioning paraffin-embedded tissues for light microscopy (correct)
- Embedding tissues in paraffin
What is the purpose of fixation in histology?
What is the purpose of fixation in histology?
- To enhance tissue visibility under the microscope
- To increase tissue flexibility
- To preserve tissues & prevent post-mortem changes (correct)
- To remove excess paraffin from tissues
What is the role of the slide cover in microscopy?
What is the role of the slide cover in microscopy?
- To provide structural support to the slide
- To prevent light from passing through the specimen
- To protect the specimen on the slide (correct)
- To enhance the magnification of the specimen
What is the function of the arm of a microscope?
What is the function of the arm of a microscope?
What is the role of the base in a light microscope?
What is the role of the base in a light microscope?
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Study Notes
Histology and Microscopy
Tissue Preparation
- A microtome is a device used to cut thin slices of tissue, typically 1-10 micrometers, for histological examination.
- Fixation is a process to preserve tissue structure and prevent decay, allowing for histological examination.
Microscopy Components
- The slide cover, also known as a coverslip, is a thin transparent plate that protects the specimen and allows for even sample illumination.
- The arm of a microscope is the vertical component that connects the microscope stage to the base, providing stability and support.
- The base of a light microscope provides a stable foundation, allowing for smooth and precise movement during observation.
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