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Cell Biology: Fractionation and Organelles

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Cell Biology: Fractionation and Organelles

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Questions and Answers

What is the primary purpose of forming a cell homogenate?

To separate organelles from one another

What type of cells frequently require the use of an abrasive during disruption?

Plant cells

What is the principle behind differential centrifugation?

Separation of organelles based on their size and density using gravity

What is the purpose of increasing the rotation rate of the centrifuge rotor in differential centrifugation?

To increase the gravitational force experienced by the homogenate Signup and view all the answers

What is the term for the explosive decompression of cells saturated with a compressed gas?

Cavitation Signup and view all the answers

What is the common characteristic shared by the two most popular methods of organelle separation?

They use gravity to separate organelles based on their size and density Signup and view all the answers

What is the meaning of the term 'fractionate'?

To separate or divide into component parts Signup and view all the answers

Why is cell fractionation important in understanding eukaryotic cells?

To discover and elucidate the functional properties of different organelles Signup and view all the answers

What is the first step in fractionating cells into organelles?

Disruption of the cells to release their contents into a benign buffer Signup and view all the answers

What is the ideal result of a fractionation procedure?

A series of fractions, each containing only one, highly purified organelle or protein Signup and view all the answers

What can be further fractionated from a purified organelle, such as mitochondria?

Its unique set of proteins, for example the enzymes of the tricarboxylic acid cycle Signup and view all the answers

What is an example of a fraction that could be obtained from a liver cell?

A fraction containing only nuclei, or one containing only mitochondria, or one containing only lysosomes Signup and view all the answers