18 Questions
What is the purpose of Electrophoresis in the laboratory?
Separate DNA, RNA, or protein molecules based on size and charge
Which laboratory technique is commonly used to detect antibodies in the blood?
ELISA
How is Hybridoma technology used in the laboratory?
Producing large numbers of identical antibodies
What is the function of Centrifugation in the molecular biology laboratory?
Separating molecules based on density by spinning at high speed
Which technique involves injecting an antigen into a mammal to provoke an immune response?
Hybridoma technology
What is the primary purpose of ELISA in the laboratory?
Detect antibodies in the blood
What is the purpose of centrifugation in the process described?
To separate components based on density or particle size
In the context of centrifugation, what happens if the subcellular component is larger?
Higher centrifugal force is required
Which method is used to separate organelles and other sub-cellular components based on sedimentation rate?
Differential centrifugation
What happens during lysis in this context?
Membranous organelles become fragmented
What is the main effect of using a dense medium in density gradient centrifugation?
Speeds up the sedimentation rate of organelles
Which technique involves filtering a homogenate solution through gauze to obtain a filtrate containing a mixture of organelles?
Filtration
What is the primary objective of cell fractionation?
To isolate components and organelles for molecular composition study
Why is it necessary to use an ice-cold solution during the extraction step of cell fractionation?
To maintain the structural integrity of the organelles by reducing enzymatic activity
Which step of cell fractionation involves placing sample tissues under mild conditions before using them?
Extraction
What is the purpose of using an isotonic buffer solution during cell fractionation?
To prevent water from moving into the organelles via osmosis
Why is it important to use a buffered solution in cell fractionation?
To prevent organelle proteins from becoming denatured
Which technique is used to separate cellular components in cell fractionation?
Centrifugation
Test your knowledge on cell fractionation techniques such as lysis, filtration, and centrifugation. Learn how organelles are separated based on density and size to obtain different components of a cell mixture.
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