Podcast
Questions and Answers
A microscope has an objective lens with 4x magnification and an ocular lens with 10x magnification. What is the total magnification?
A microscope has an objective lens with 4x magnification and an ocular lens with 10x magnification. What is the total magnification?
- 4
- 14
- 40 (correct)
- 10
Which of the following is the primary advantage of using a scanning power (e.g., 4x objective lens) on a microscope?
Which of the following is the primary advantage of using a scanning power (e.g., 4x objective lens) on a microscope?
- Ability to view the entire slide to locate specific areas of interest (correct)
- Maximum magnification for observing subcellular organelles
- Highest possible resolution of cellular structures
- Narrow field of view for detailed examination
During which phase of mitosis do the sister chromatids separate and move towards opposite poles of the cell?
During which phase of mitosis do the sister chromatids separate and move towards opposite poles of the cell?
- Metaphase
- Anaphase (correct)
- Prophase
- Telophase
Given a microscopic image displaying epithelial cells with nuclei aligned in a single layer and columnar shape, but lacking surface projections, which of the following classifications is the MOST accurate?
Given a microscopic image displaying epithelial cells with nuclei aligned in a single layer and columnar shape, but lacking surface projections, which of the following classifications is the MOST accurate?
Which characteristic primarily differentiates telophase from cytokinesis?
Which characteristic primarily differentiates telophase from cytokinesis?
Epithelial cells lining the female reproductive tract (specifically, the uterine tubes) are characterized by cilia on their apical surface. What is MOST likely the primary function of these cilia in this location?
Epithelial cells lining the female reproductive tract (specifically, the uterine tubes) are characterized by cilia on their apical surface. What is MOST likely the primary function of these cilia in this location?
A cell is placed in a solution, and water moves out of the cell, causing it to shrink. Which of the following best describes the solution relative to the cell?
A cell is placed in a solution, and water moves out of the cell, causing it to shrink. Which of the following best describes the solution relative to the cell?
In a microscopic observation of a tissue sample, you notice cells that appear to have multiple layers of nuclei at various heights, but upon closer examination, all cells are in contact with the basement membrane. Which of the following classifications BEST describes this tissue?
In a microscopic observation of a tissue sample, you notice cells that appear to have multiple layers of nuclei at various heights, but upon closer examination, all cells are in contact with the basement membrane. Which of the following classifications BEST describes this tissue?
Which process describes the movement of solute molecules from an area of high concentration to an area of low concentration?
Which process describes the movement of solute molecules from an area of high concentration to an area of low concentration?
Why are large molecules like starch typically unable to cross a cell membrane through simple diffusion?
Why are large molecules like starch typically unable to cross a cell membrane through simple diffusion?
Goblet cells are frequently observed within certain types of epithelia. What is the primary function of goblet cells within these tissues?
Goblet cells are frequently observed within certain types of epithelia. What is the primary function of goblet cells within these tissues?
What type of tissue is characterized by cells that are specialized to conduct electrical impulses?
What type of tissue is characterized by cells that are specialized to conduct electrical impulses?
If a tissue sample is described as 'pseudostratified,' what key feature distinguishes it from truly stratified epithelium?
If a tissue sample is described as 'pseudostratified,' what key feature distinguishes it from truly stratified epithelium?
Which characteristic BEST differentiates simple columnar epithelium found in the stomach from simple columnar epithelium found in the uterine tubes?
Which characteristic BEST differentiates simple columnar epithelium found in the stomach from simple columnar epithelium found in the uterine tubes?
What is the functional significance of the 'squidged' appearance of nuclei in pseudostratified columnar epithelium?
What is the functional significance of the 'squidged' appearance of nuclei in pseudostratified columnar epithelium?
In the context of the female reproductive tract, if the ciliated simple columnar epithelium were damaged or non-functional, what is the MOST likely consequence?
In the context of the female reproductive tract, if the ciliated simple columnar epithelium were damaged or non-functional, what is the MOST likely consequence?
If a simple squamous epithelium in the lungs were damaged, what immediate physiological consequence would be MOST likely to occur?
If a simple squamous epithelium in the lungs were damaged, what immediate physiological consequence would be MOST likely to occur?
What key adaptation allows transitional epithelium to effectively accommodate significant changes in organ volume, such as in the bladder?
What key adaptation allows transitional epithelium to effectively accommodate significant changes in organ volume, such as in the bladder?
Which characteristic of keratinized stratified squamous epithelium is MOST critical for its protective function in the skin?
Which characteristic of keratinized stratified squamous epithelium is MOST critical for its protective function in the skin?
What is the functional significance of having both collagen and elastic fibers within areolar connective tissue?
What is the functional significance of having both collagen and elastic fibers within areolar connective tissue?
What is the MAIN role of cilia in pseudostratified ciliated columnar epithelium found in the trachea?
What is the MAIN role of cilia in pseudostratified ciliated columnar epithelium found in the trachea?
In the context of the kidney, why is simple cuboidal epithelium particularly well-suited for filtration processes?
In the context of the kidney, why is simple cuboidal epithelium particularly well-suited for filtration processes?
What is the PRIMARY determinant of whether an epithelium is classified as simple or stratified?
What is the PRIMARY determinant of whether an epithelium is classified as simple or stratified?
What is a critical functional consequence of losing cells from a single-layered epithelium compared to a multi-layered epithelium?
What is a critical functional consequence of losing cells from a single-layered epithelium compared to a multi-layered epithelium?
How does the arrangement of adipocytes in adipose tissue contribute to its function in protecting organs such as the kidneys?
How does the arrangement of adipocytes in adipose tissue contribute to its function in protecting organs such as the kidneys?
If a tissue sample from the uterus is described as stratified squamous epithelium, what specific functional requirement does this structural characteristic likely address?
If a tissue sample from the uterus is described as stratified squamous epithelium, what specific functional requirement does this structural characteristic likely address?
A researcher is examining a tissue sample and observes cells with nuclei that appear as small dots within large, clear areas. This is MOST indicative of which cell type?
A researcher is examining a tissue sample and observes cells with nuclei that appear as small dots within large, clear areas. This is MOST indicative of which cell type?
How might a disruption in the elastic fibers within the dermis of the skin manifest physically?
How might a disruption in the elastic fibers within the dermis of the skin manifest physically?
If the collagen fibers in areolar connective tissue were selectively degraded, which of the following functional deficits would MOST likely result?
If the collagen fibers in areolar connective tissue were selectively degraded, which of the following functional deficits would MOST likely result?
In a scenario where the simple cuboidal epithelium of the kidney tubules is damaged, leading to impaired filtration, what compensatory mechanism might the body employ to maintain homeostasis?
In a scenario where the simple cuboidal epithelium of the kidney tubules is damaged, leading to impaired filtration, what compensatory mechanism might the body employ to maintain homeostasis?
Considering the structural properties of transitional epithelium, what cellular process is MOST directly responsible for its ability to accommodate stretching in the bladder?
Considering the structural properties of transitional epithelium, what cellular process is MOST directly responsible for its ability to accommodate stretching in the bladder?
Which of the following represents the most accurate sequence of components involved in maintaining homeostasis?
Which of the following represents the most accurate sequence of components involved in maintaining homeostasis?
A researcher is studying the homeostatic response to increased blood glucose levels. If the pancreas is the effector, what would be the direct stimulus initiating this homeostatic mechanism?
A researcher is studying the homeostatic response to increased blood glucose levels. If the pancreas is the effector, what would be the direct stimulus initiating this homeostatic mechanism?
If the parietal pleura is damaged, which structure would be most immediately affected?
If the parietal pleura is damaged, which structure would be most immediately affected?
A surgeon needs to make an incision that divides the kidney into anterior and posterior portions. Which anatomical plane should the surgeon use?
A surgeon needs to make an incision that divides the kidney into anterior and posterior portions. Which anatomical plane should the surgeon use?
During a clinical examination, a doctor observes that a patient's elbow is positioned superior to their wrist. Given this information, which of the following statements must also be true?
During a clinical examination, a doctor observes that a patient's elbow is positioned superior to their wrist. Given this information, which of the following statements must also be true?
After conducting a food testing lab, a student observes a color change in a sample treated with Benedict's reagent. What specific conclusion can be drawn from this observation?
After conducting a food testing lab, a student observes a color change in a sample treated with Benedict's reagent. What specific conclusion can be drawn from this observation?
A researcher is preparing a sample for microscopic examination and needs to finely adjust the focus at high magnification. Which component of the microscope should they use?
A researcher is preparing a sample for microscopic examination and needs to finely adjust the focus at high magnification. Which component of the microscope should they use?
In a practical exam, an image displays a tissue sample with cells arranged in multiple layers, where the cells at the basal layer are cuboidal and progressively flatten towards the surface. Which type of epithelium is most likely shown in the image?
In a practical exam, an image displays a tissue sample with cells arranged in multiple layers, where the cells at the basal layer are cuboidal and progressively flatten towards the surface. Which type of epithelium is most likely shown in the image?
A histology student is examining a slide and observes a tissue with collagen fibers aligned parallel to each other. Which type of connective tissue is the student most likely observing?
A histology student is examining a slide and observes a tissue with collagen fibers aligned parallel to each other. Which type of connective tissue is the student most likely observing?
During a lab practical, you are presented with a slide showing nervous tissue. Which of the following features would definitively identify it as nervous tissue rather than another type?
During a lab practical, you are presented with a slide showing nervous tissue. Which of the following features would definitively identify it as nervous tissue rather than another type?
A student is asked to identify a tissue sample under a microscope. The cells are hexagonal, tightly packed, and appear to form a honeycomb-like structure. Which type of tissue is the student most likely observing?
A student is asked to identify a tissue sample under a microscope. The cells are hexagonal, tightly packed, and appear to form a honeycomb-like structure. Which type of tissue is the student most likely observing?
Which of the adjustments to the microscope will increase the amount of light reaching the specimen?
Which of the adjustments to the microscope will increase the amount of light reaching the specimen?
A researcher is examining a tissue sample that consists of cells suspended in a fluid matrix. The matrix contains collagen and elastin fibers, and the cells appear to be mobile. Which specific type of connective tissue is the researcher most likely observing?
A researcher is examining a tissue sample that consists of cells suspended in a fluid matrix. The matrix contains collagen and elastin fibers, and the cells appear to be mobile. Which specific type of connective tissue is the researcher most likely observing?
In a practical exam, a slide shows a single layer of elongated cells with the nuclei located near the base and cilia on the apical surface. What type of epithelium is most likely being observed?
In a practical exam, a slide shows a single layer of elongated cells with the nuclei located near the base and cilia on the apical surface. What type of epithelium is most likely being observed?
A student performs a food test using Biuret reagent and observes a violet color change. This result indicates the presence of which macromolecule?
A student performs a food test using Biuret reagent and observes a violet color change. This result indicates the presence of which macromolecule?
In connective tissues, what is the primary functional difference between dense regular and dense irregular connective tissues?
In connective tissues, what is the primary functional difference between dense regular and dense irregular connective tissues?
Why is it important to avoid using abbreviations like 'RBC' or 'WBC' when identifying cell types in blood samples in a practical setting?
Why is it important to avoid using abbreviations like 'RBC' or 'WBC' when identifying cell types in blood samples in a practical setting?
How does the structure of reticular connective tissue in the spleen contribute to its function of filtering out old red blood cells?
How does the structure of reticular connective tissue in the spleen contribute to its function of filtering out old red blood cells?
What is the functional significance of the elastic fibers present in elastic cartilage, such as that found in the ear?
What is the functional significance of the elastic fibers present in elastic cartilage, such as that found in the ear?
In the context of bone tissue, what is the critical role of blood vessels that penetrate the compact bone?
In the context of bone tissue, what is the critical role of blood vessels that penetrate the compact bone?
How do intercalated discs contribute to the coordinated function of cardiac muscle cells in the heart?
How do intercalated discs contribute to the coordinated function of cardiac muscle cells in the heart?
If a patient is diagnosed with osteoarthritis, and it is found that the hyaline cartilage at the end of their long bones has eroded, what is the primary consequence of this erosion?
If a patient is diagnosed with osteoarthritis, and it is found that the hyaline cartilage at the end of their long bones has eroded, what is the primary consequence of this erosion?
What is the primary role of platelets (thrombocytes) present in blood, and how do they achieve this function?
What is the primary role of platelets (thrombocytes) present in blood, and how do they achieve this function?
How does the multi-nucleated structure of skeletal muscle cells directly contribute to their function?
How does the multi-nucleated structure of skeletal muscle cells directly contribute to their function?
What is the main distinguishing factor between smooth muscle and skeletal muscle when viewed under a microscope?
What is the main distinguishing factor between smooth muscle and skeletal muscle when viewed under a microscope?
What is the role of fibrocartilage found in locations such as between the vertebrae and the pubic symphysis?
What is the role of fibrocartilage found in locations such as between the vertebrae and the pubic symphysis?
What structural features differentiate a neuron, and name the functions?
What structural features differentiate a neuron, and name the functions?
How does the adipose tissue surrounding the heart contribute to cardiac function?
How does the adipose tissue surrounding the heart contribute to cardiac function?
Considering the locations of hyaline cartilage, fibrocartilage, and elastic cartilage, which of the following statements is most accurate?
Considering the locations of hyaline cartilage, fibrocartilage, and elastic cartilage, which of the following statements is most accurate?
During the microscopic analysis of a tissue sample, you observe cells with multiple nuclei and distinct striations. How would you classify this tissue, and what is its primary function?
During the microscopic analysis of a tissue sample, you observe cells with multiple nuclei and distinct striations. How would you classify this tissue, and what is its primary function?
Flashcards
Simple Columnar Epithelium
Simple Columnar Epithelium
A single layer of column-shaped cells with nuclei aligned at the base.
Non-ciliated Simple Columnar Epithelium
Non-ciliated Simple Columnar Epithelium
Simple columnar epithelium without cilia on its surface.
Ciliated Simple Columnar Epithelium
Ciliated Simple Columnar Epithelium
A single layer of column-shaped cells with cilia on their apical surface.
Cilia
Cilia
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Goblet Cells
Goblet Cells
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Pseudostratified Epithelium
Pseudostratified Epithelium
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Apical Surface
Apical Surface
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Epithelial Identification
Epithelial Identification
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Ciliated Pseudostratified Epithelium Function
Ciliated Pseudostratified Epithelium Function
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Simple Squamous Epithelium
Simple Squamous Epithelium
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Simple Squamous Function
Simple Squamous Function
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Simple Cuboidal Epithelium
Simple Cuboidal Epithelium
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Simple Cuboidal Function
Simple Cuboidal Function
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Transitional Epithelium
Transitional Epithelium
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Stratified Squamous Epithelium
Stratified Squamous Epithelium
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Stratified Epithelium Function
Stratified Epithelium Function
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Keratinized Stratified Squamous
Keratinized Stratified Squamous
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Keratinized Epithelium Function
Keratinized Epithelium Function
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Areolar Connective Tissue
Areolar Connective Tissue
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Collagen Function (Areolar)
Collagen Function (Areolar)
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Elastic Fiber Function (Areolar)
Elastic Fiber Function (Areolar)
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Adipose Tissue
Adipose Tissue
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Total Magnification
Total Magnification
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Scanning Power
Scanning Power
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High Power
High Power
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Metaphase
Metaphase
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Anaphase
Anaphase
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Telophase
Telophase
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Cytokinesis
Cytokinesis
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Osmosis
Osmosis
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Reticular Connective Tissue
Reticular Connective Tissue
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Dense Regular Connective Tissue
Dense Regular Connective Tissue
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Dense Irregular Connective Tissue
Dense Irregular Connective Tissue
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Hyaline Cartilage
Hyaline Cartilage
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Fibrocartilage
Fibrocartilage
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Elastic Cartilage
Elastic Cartilage
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Compact Bone
Compact Bone
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Blood
Blood
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Red Blood Cells (Erythrocytes)
Red Blood Cells (Erythrocytes)
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White Blood Cells (Leukocytes)
White Blood Cells (Leukocytes)
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Platelets
Platelets
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Skeletal Muscle
Skeletal Muscle
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Cardiac Muscle
Cardiac Muscle
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Smooth Muscle
Smooth Muscle
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Neuron
Neuron
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Homeostasis
Homeostasis
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Stimulus (Homeostasis)
Stimulus (Homeostasis)
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Sensor (Homeostasis)
Sensor (Homeostasis)
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Integration Center (Homeostasis)
Integration Center (Homeostasis)
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Effector (Homeostasis)
Effector (Homeostasis)
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Visceral Membrane
Visceral Membrane
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Parietal Membrane
Parietal Membrane
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Main Organs (Examples)
Main Organs (Examples)
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Lateral
Lateral
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Distal
Distal
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Proximal
Proximal
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Frontal (Coronal) Plane
Frontal (Coronal) Plane
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Sagittal Plane
Sagittal Plane
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Transverse Plane
Transverse Plane
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Benedict's Reagent
Benedict's Reagent
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Study Notes
Histology Practical - Epithelial Tissue
- For epithelial cells always identify the free space first
- Look down to find the cells of interest
- Simple columnar epithelium has nuclei in a perfect line at the top layer of cells
- The cells are column-shaped with a single layer of nuclei
Simple Columnar Epithelium
- Non-ciliated simple columnar epithelium does not have projections
- This tissue is found in the stomach
- Just putting simple columnar as the answer to a practical question is acceptable
- Ciliated simple columnar epithelium has brush-like projections (cilia)
- Cilia are for movement and movement facilitation
- This tissue is in the female reproductive tract (uterine tubes)
- Cilia helps move the oocyte down and sperm up
- Goblet cells (black lines) secrete mucus for lubrication
Pseudo-stratified Columnar Epithelium
- The tissue tricks the eye into thinking there's more than one layer
- Nuclei are squished giving the impression of multiple layers
- Despite the appearance, it is a single layer
- Cilia can be seen blowing in the breeze on the surface
- Trachea (windpipe) uses cilia to trap dust and debris
Simple Squamous Epithelium
- Difficult to identify looks like Adipose cells
- The nuclei appear as small dots
- The cells are drawn out
- This tissue is found in the lungs
- Its one layer minimizes the mass of cells in the lungs
Simple Cuboidal Epithelium
- Has small holes (luminal spaces)
- Cells directly around the luminal space are of interest
- Found in the kidney
- Fluid is filtered through the luminal spaces
- Filtration is more efficient through one layer of cells
Transitional Epithelium
- Features transitioning cell types
- Dome-shaped cells are squat and not elongated
- Deeper cells are elongated
- Stretching occurs as the bladder expands and contracts gradually over time
- This tissue type is found in the bladder
Stratified Squamous Epithelium
- Has multiple layers of epithelial cells
- Found in the uterus, specifically the endometrial lining
- Multiple layers provide protection from erosion
- Cells lost in the space don't matter due to multiple layers
- Multiple layers are for cases where abrasion erosion occurs
Keratinized Stratified Squamous Epithelium
- It's skin
- The top layer is keratin (protective protein)
- Protection is the key function
- Protects deeper layers and connective tissue from damage
Connective Tissue - Areolar
- Wishbone shape is collagen protein
- Squiggly, wiggly shapes are elastic fibers
- Found in the dermis of the skin
- Elastic fibers provide stretchiness (elasticity)
- Collagen provides toughness
Connective Tissue - Adipose
- The cell type is adipocytes
- Small specs are the nuclei of the cells
- Found around kidneys for protection and support
- Location includes around the heart as well for protection
Connective Tissue - Reticular
- Location is the spleen
- Network of fibers traps and filters
- Important for filtering
- Can be found in the lymph nodes
Connective Tissue - Dense Regular
- Fibers go in the same direction
- Found in joints
- Provides unidirectional strength
Connective Tissue - Dense Irregular
- Fibers go every which way
- Provides multi-directional strength
- Found in the dermis
Connective Tissue - Hyaline Cartilage
- Found around the ends of long bones (e.g., femur to tibia)
- Prevents bone from contacting bone
- Erosion can lead to bone-on-bone contact (osteoarthritis)
Connective Tissue - Fibrocartilage
- Found between the vertebrae
- Also between parts of the pelvis (pubic symphysis)
Connective Tissue - Elastic Cartilage
- Has elastic fibers
- Found in the ears
Connective Tissue - Bone
- It's compact bone
- Located in bones
- Functions to work with muscle for movement
- Blood vessels come in through the middle
- Bone is an active tissue that needs to be nourished
Connective Tissue - Blood
- Lots of red blood cells
- Cell types:
- Red blood cells (erythrocytes)
- White blood cells (leukocytes)
- Platelets
- Red blood cells carry oxygen, white blood cells fight infection, and platelets clot blood
Muscle Tissue - Skeletal
- Obvious striations (markings)
- Multi-nucleated (many nuclei)
- Enables movement
Muscle Tissue - Cardiac
- Has lines (striations)
- Has inter calculated discs (junctions) going across the cells
Muscle Tissue - Smooth
- Doesn't have striations
- Found in organs (stomach, intestines)
- Involuntarily controlled
Nervous Tissue - Neuron
- Large purple cell with projections (dendrites and axon)
- The large purple part is the soma (cell body)
Practical Exam Review
Homeostasis
- Definition of homeostasis must be known
- Give examples such as temperature regulation or blood pressure
- Know the role of each component:
- Stimulus: trigger
- Sensor: detects information
- Integration: brain or spinal cord determines what to do
- Effector: drives output
- Response: body sorts itself out
Membranes
- Name the membranes (visceral and parietal layers) of the heart (pericardium) and lungs
- Name the organ in a picture
- Organs to know: lungs, heart, liver, stomach, intestines, pancreas, kidneys, spleen
- Take pictures of the torso models
General Body Parts and Orientation
- Eyes, ears, nose, elbow, shoulder
- Ears are lateral to the nose
- Hand is distal to the elbow
- Know proximal, distal, superior, inferior, anterior, posterior
Slices
- Frontal, sagittal, transverse, oblique
- Important consideration for practical questions
Food Testing Lab
- Know what the different indicators test for:
- Benedict's: glucose
- Lugol's/iodine: starch
- Biuret: protein
- Don't need to know the colors they change
Microscopy
- Know the basic anatomy of the scope: ocular, objectives, course and fine adjustment, stage, iris diaphragm, light source
- Know how to calculate total magnification such as 4x * 10 eye piece = 40x magnification
- Scanning power (4x) gives a wide field of vision to scan the whole slide
- Higher power (40x) gives greater resolution, more magnification, but the field of view is reduced
Mitosis
- Name the stage displayed in images of cells in different stages of mitosis:
- Interphase
- Prophase
- Metaphase
- Anaphase
- Telophase
- Cytokinesis
Osmosis and Diffusion
- Osmosis: movement of water
- Diffusion: movement of solutes
- Large molecules (e.g., starch) cannot move across the membrane because they are too big
- Smaller molecules (e.g., ions) can diffuse through the membrane
Practical Exam Details
- 78 questions
- Questions 20-60 (40 questions) are histology images of epithelial and connective tissue (majority of the exam)
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