Cell Biology Concepts

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Questions and Answers

A microscope has an objective lens with 4x magnification and an ocular lens with 10x magnification. What is the total magnification?

  • 4
  • 14
  • 40 (correct)
  • 10

Which of the following is the primary advantage of using a scanning power (e.g., 4x objective lens) on a microscope?

  • Ability to view the entire slide to locate specific areas of interest (correct)
  • Maximum magnification for observing subcellular organelles
  • Highest possible resolution of cellular structures
  • Narrow field of view for detailed examination

During which phase of mitosis do the sister chromatids separate and move towards opposite poles of the cell?

  • Metaphase
  • Anaphase (correct)
  • Prophase
  • Telophase

Given a microscopic image displaying epithelial cells with nuclei aligned in a single layer and columnar shape, but lacking surface projections, which of the following classifications is the MOST accurate?

<p>Simple Columnar Epithelium (A)</p> Signup and view all the answers

Which characteristic primarily differentiates telophase from cytokinesis?

<p>Formation of two distinct daughter cells (D)</p> Signup and view all the answers

Epithelial cells lining the female reproductive tract (specifically, the uterine tubes) are characterized by cilia on their apical surface. What is MOST likely the primary function of these cilia in this location?

<p>To facilitate the movement of the egg cell and sperm. (C)</p> Signup and view all the answers

A cell is placed in a solution, and water moves out of the cell, causing it to shrink. Which of the following best describes the solution relative to the cell?

<p>Hypertonic (D)</p> Signup and view all the answers

In a microscopic observation of a tissue sample, you notice cells that appear to have multiple layers of nuclei at various heights, but upon closer examination, all cells are in contact with the basement membrane. Which of the following classifications BEST describes this tissue?

<p>Pseudostratified Columnar Epithelium (A)</p> Signup and view all the answers

Which process describes the movement of solute molecules from an area of high concentration to an area of low concentration?

<p>Diffusion (C)</p> Signup and view all the answers

Why are large molecules like starch typically unable to cross a cell membrane through simple diffusion?

<p>They are too large to pass through the membrane's selective pores. (A)</p> Signup and view all the answers

Goblet cells are frequently observed within certain types of epithelia. What is the primary function of goblet cells within these tissues?

<p>Producing and secreting mucus for lubrication and protection. (D)</p> Signup and view all the answers

What type of tissue is characterized by cells that are specialized to conduct electrical impulses?

<p>Nervous tissue (B)</p> Signup and view all the answers

If a tissue sample is described as 'pseudostratified,' what key feature distinguishes it from truly stratified epithelium?

<p>The appearance of multiple layers of cells, but all cells are attached to the basement membrane. (A)</p> Signup and view all the answers

Which characteristic BEST differentiates simple columnar epithelium found in the stomach from simple columnar epithelium found in the uterine tubes?

<p>The presence of microvilli in the stomach epithelium to increase absorption versus the presence of cilia in the uterine tubes to facilitate movement. (A)</p> Signup and view all the answers

What is the functional significance of the 'squidged' appearance of nuclei in pseudostratified columnar epithelium?

<p>It contributes to the illusion of multiple cell layers within a single-layered tissue. (C)</p> Signup and view all the answers

In the context of the female reproductive tract, if the ciliated simple columnar epithelium were damaged or non-functional, what is the MOST likely consequence?

<p>Impaired movement of oocytes towards the uterus, potentially affecting fertility. (C)</p> Signup and view all the answers

If a simple squamous epithelium in the lungs were damaged, what immediate physiological consequence would be MOST likely to occur?

<p>Compromised gas exchange efficiency due to increased diffusion distance. (C)</p> Signup and view all the answers

What key adaptation allows transitional epithelium to effectively accommodate significant changes in organ volume, such as in the bladder?

<p>The ability of cells to change shape from dome-like to flattened, allowing for stretching. (A)</p> Signup and view all the answers

Which characteristic of keratinized stratified squamous epithelium is MOST critical for its protective function in the skin?

<p>The presence of a waterproof, abrasion-resistant layer of keratin. (C)</p> Signup and view all the answers

What is the functional significance of having both collagen and elastic fibers within areolar connective tissue?

<p>Collagen contributes strength and toughness, while elastic fibers allow for stretch and recoil. (D)</p> Signup and view all the answers

What is the MAIN role of cilia in pseudostratified ciliated columnar epithelium found in the trachea?

<p>To propel mucus containing trapped particles away from the lungs. (B)</p> Signup and view all the answers

In the context of the kidney, why is simple cuboidal epithelium particularly well-suited for filtration processes?

<p>Its single layer facilitates efficient fluid passage and selective absorption. (C)</p> Signup and view all the answers

What is the PRIMARY determinant of whether an epithelium is classified as simple or stratified?

<p>The number of cell layers between the basement membrane and the apical surface. (C)</p> Signup and view all the answers

What is a critical functional consequence of losing cells from a single-layered epithelium compared to a multi-layered epithelium?

<p>Cell loss in single-layered epithelia directly exposes underlying connective tissue. (C)</p> Signup and view all the answers

How does the arrangement of adipocytes in adipose tissue contribute to its function in protecting organs such as the kidneys?

<p>Adipocytes provide cushioning and insulation, protecting the kidneys from physical trauma and temperature fluctuations. (D)</p> Signup and view all the answers

If a tissue sample from the uterus is described as stratified squamous epithelium, what specific functional requirement does this structural characteristic likely address?

<p>Providing protection against abrasion and erosion in the uterine lining. (C)</p> Signup and view all the answers

A researcher is examining a tissue sample and observes cells with nuclei that appear as small dots within large, clear areas. This is MOST indicative of which cell type?

<p>Adipocytes within adipose tissue. (A)</p> Signup and view all the answers

How might a disruption in the elastic fibers within the dermis of the skin manifest physically?

<p>Reduced ability of the skin to return to its original shape after stretching. (C)</p> Signup and view all the answers

If the collagen fibers in areolar connective tissue were selectively degraded, which of the following functional deficits would MOST likely result?

<p>A decreased ability of the tissue to resist tensile forces. (D)</p> Signup and view all the answers

In a scenario where the simple cuboidal epithelium of the kidney tubules is damaged, leading to impaired filtration, what compensatory mechanism might the body employ to maintain homeostasis?

<p>Increase blood pressure to force more fluid through the remaining functional filtration units. (B)</p> Signup and view all the answers

Considering the structural properties of transitional epithelium, what cellular process is MOST directly responsible for its ability to accommodate stretching in the bladder?

<p>Alterations in cell shape facilitated by changes in the cytoskeleton. (B)</p> Signup and view all the answers

Which of the following represents the most accurate sequence of components involved in maintaining homeostasis?

<p>Stimulus, Sensor, Integration, Effector, Response (D)</p> Signup and view all the answers

A researcher is studying the homeostatic response to increased blood glucose levels. If the pancreas is the effector, what would be the direct stimulus initiating this homeostatic mechanism?

<p>Elevated blood glucose levels detected by pancreatic islet cells. (B)</p> Signup and view all the answers

If the parietal pleura is damaged, which structure would be most immediately affected?

<p>The inner lining of the thoracic cavity. (D)</p> Signup and view all the answers

A surgeon needs to make an incision that divides the kidney into anterior and posterior portions. Which anatomical plane should the surgeon use?

<p>Frontal (coronal) plane (A)</p> Signup and view all the answers

During a clinical examination, a doctor observes that a patient's elbow is positioned superior to their wrist. Given this information, which of the following statements must also be true?

<p>The wrist is inferior to the elbow. (C)</p> Signup and view all the answers

After conducting a food testing lab, a student observes a color change in a sample treated with Benedict's reagent. What specific conclusion can be drawn from this observation?

<p>The sample contains reducing sugars such as glucose. (D)</p> Signup and view all the answers

A researcher is preparing a sample for microscopic examination and needs to finely adjust the focus at high magnification. Which component of the microscope should they use?

<p>Fine adjustment knob (D)</p> Signup and view all the answers

In a practical exam, an image displays a tissue sample with cells arranged in multiple layers, where the cells at the basal layer are cuboidal and progressively flatten towards the surface. Which type of epithelium is most likely shown in the image?

<p>Stratified squamous epithelium (A)</p> Signup and view all the answers

A histology student is examining a slide and observes a tissue with collagen fibers aligned parallel to each other. Which type of connective tissue is the student most likely observing?

<p>Dense regular connective tissue (B)</p> Signup and view all the answers

During a lab practical, you are presented with a slide showing nervous tissue. Which of the following features would definitively identify it as nervous tissue rather than another type?

<p>Presence of neurons with visible axons (C)</p> Signup and view all the answers

A student is asked to identify a tissue sample under a microscope. The cells are hexagonal, tightly packed, and appear to form a honeycomb-like structure. Which type of tissue is the student most likely observing?

<p>Adipose tissue (B)</p> Signup and view all the answers

Which of the adjustments to the microscope will increase the amount of light reaching the specimen?

<p>Opening the iris diaphragm (C)</p> Signup and view all the answers

A researcher is examining a tissue sample that consists of cells suspended in a fluid matrix. The matrix contains collagen and elastin fibers, and the cells appear to be mobile. Which specific type of connective tissue is the researcher most likely observing?

<p>Blood (C)</p> Signup and view all the answers

In a practical exam, a slide shows a single layer of elongated cells with the nuclei located near the base and cilia on the apical surface. What type of epithelium is most likely being observed?

<p>Pseudostratified columnar epithelium (C)</p> Signup and view all the answers

A student performs a food test using Biuret reagent and observes a violet color change. This result indicates the presence of which macromolecule?

<p>Proteins (D)</p> Signup and view all the answers

In connective tissues, what is the primary functional difference between dense regular and dense irregular connective tissues?

<p>Dense regular connective tissue provides unidirectional strength, whereas dense irregular connective tissue offers strength in multiple directions. (C)</p> Signup and view all the answers

Why is it important to avoid using abbreviations like 'RBC' or 'WBC' when identifying cell types in blood samples in a practical setting?

<p>Abbreviations are considered unprofessional and do not fully demonstrate understanding of the cell types. (A)</p> Signup and view all the answers

How does the structure of reticular connective tissue in the spleen contribute to its function of filtering out old red blood cells?

<p>The net-like network of fibers in reticular tissue provides a framework that traps and filters out old red blood cells. (D)</p> Signup and view all the answers

What is the functional significance of the elastic fibers present in elastic cartilage, such as that found in the ear?

<p>The elastic fibers allow the cartilage to be stretched and deformed, then return to its original shape. (D)</p> Signup and view all the answers

In the context of bone tissue, what is the critical role of blood vessels that penetrate the compact bone?

<p>They facilitate the exchange of oxygen and nutrients to keep bone cells alive and functioning. (D)</p> Signup and view all the answers

How do intercalated discs contribute to the coordinated function of cardiac muscle cells in the heart?

<p>They facilitate rapid electrical communication, allowing the heart muscle cells to contract in a coordinated manner. (C)</p> Signup and view all the answers

If a patient is diagnosed with osteoarthritis, and it is found that the hyaline cartilage at the end of their long bones has eroded, what is the primary consequence of this erosion?

<p>Pain and inflammation due to bone-on-bone contact within the joint. (A)</p> Signup and view all the answers

What is the primary role of platelets (thrombocytes) present in blood, and how do they achieve this function?

<p>They facilitate blood clotting by aggregating at the site of vessel injury. (D)</p> Signup and view all the answers

How does the multi-nucleated structure of skeletal muscle cells directly contribute to their function?

<p>The multiple nuclei increase the cell's capacity to produce proteins required for muscle contraction and repair. (C)</p> Signup and view all the answers

What is the main distinguishing factor between smooth muscle and skeletal muscle when viewed under a microscope?

<p>The presence of striations (stripes) in skeletal muscle, which are absent in smooth muscle. (A)</p> Signup and view all the answers

What is the role of fibrocartilage found in locations such as between the vertebrae and the pubic symphysis?

<p>To provide strong support and withstand heavy pressure. (A)</p> Signup and view all the answers

What structural features differentiate a neuron, and name the functions?

<p>Soma, axons, and dendrites; transmits electrical signals. (C)</p> Signup and view all the answers

How does the adipose tissue surrounding the heart contribute to cardiac function?

<p>It provides a protective cushion and insulation to the heart, while also serving as an energy reserve. (D)</p> Signup and view all the answers

Considering the locations of hyaline cartilage, fibrocartilage, and elastic cartilage, which of the following statements is most accurate?

<p>Long bone ends need minimal friction, the outer ear needs flexibility, intervertebral discs need resistance of stress. (C)</p> Signup and view all the answers

During the microscopic analysis of a tissue sample, you observe cells with multiple nuclei and distinct striations. How would you classify this tissue, and what is its primary function?

<p>Skeletal muscle; voluntary movements. (D)</p> Signup and view all the answers

Flashcards

Simple Columnar Epithelium

A single layer of column-shaped cells with nuclei aligned at the base.

Non-ciliated Simple Columnar Epithelium

Simple columnar epithelium without cilia on its surface.

Ciliated Simple Columnar Epithelium

A single layer of column-shaped cells with cilia on their apical surface.

Cilia

Hair-like projections on the apical surface of some epithelial cells, used for movement across the cell surface.

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Goblet Cells

Cells that secrete mucus, often found among epithelial cells.

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Pseudostratified Epithelium

Epithelium that appears to have multiple layers but is, in fact, a single layer of cells.

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Apical Surface

The surface of an epithelial cell that is exposed to the external environment or a body cavity.

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Epithelial Identification

Epithelial cells, spot the free space, look down those cells that you come to.Those are the ones that you're interested in.

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Ciliated Pseudostratified Epithelium Function

Located in the trachea, it traps dust and debris with its cilia.

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Simple Squamous Epithelium

A single layer of flattened cells, found in the lungs.

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Simple Squamous Function

Minimizes cell mass for efficient gas exchange in the lungs.

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Simple Cuboidal Epithelium

A single layer of cube-shaped cells, found in the kidney.

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Simple Cuboidal Function

Facilitates filtration through a single layer of cells in the kidney.

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Transitional Epithelium

Epithelium with transitioning cell types that can stretch, found in the bladder.

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Stratified Squamous Epithelium

Multiple layers of cells, with the top layer having a squamous shape.

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Stratified Epithelium Function

Provides protection from abrasion; multiple layers can replace lost surface cells.

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Keratinized Stratified Squamous

Stratified squamous epithelium with a keratin protein layer on the surface.

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Keratinized Epithelium Function

Provides protection to the epidermis of the skin.

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Areolar Connective Tissue

Connective tissue with collagen and elastic fibers.

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Collagen Function (Areolar)

Provides toughness to the areolar connective tissue.

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Elastic Fiber Function (Areolar)

Provides elasticity to the areolar connective tissue.

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Adipose Tissue

Connective tissue composed of fat cells (adipocytes).

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Total Magnification

Total magnification is calculated by multiplying the objective lens power by the ocular lens power.

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Scanning Power

Lower magnification provides a wider field of view, useful for scanning a whole slide.

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High Power

Higher magnification provides greater resolution and more detail, but reduces the field of view.

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Metaphase

A stage of mitosis where chromosomes line up in the middle of the cell.

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Anaphase

A stage of mitosis where sister chromatids are pulled apart towards opposite poles of the cell.

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Telophase

The final stage of mitosis, where two distinct cells form with new nuclear membranes.

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Cytokinesis

The division of the cytoplasm, resulting in two separate daughter cells.

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Osmosis

The movement of water across a semipermeable membrane from an area of high water concentration to low water concentration.

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Reticular Connective Tissue

Connective tissue with fibers arranged like a net, important for trapping and filtering substances.

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Dense Regular Connective Tissue

Connective tissue with fibers aligned in the same direction providing strength in one direction

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Dense Irregular Connective Tissue

Connective tissue with fibers arranged in multiple directions, providing strength.

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Hyaline Cartilage

Connective tissue found at the ends of long bones, preventing bone-on-bone contact.

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Fibrocartilage

Connective tissue found between vertebrae and in the pubic symphysis.

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Elastic Cartilage

Cartilage containing elastic fibers, found in the ears.

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Compact Bone

Type of bone tissue that is dense and compact.

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Blood

Connective tissue consisting of red blood cells, white blood cells, and platelets.

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Red Blood Cells (Erythrocytes)

A component of blood that carries oxygen.

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White Blood Cells (Leukocytes)

Component of blood that fights infection.

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Platelets

Cell fragments in blood that are involved in clotting.

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Skeletal Muscle

Muscle tissue with striations and multiple nuclei per cell, enabling voluntary movement.

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Cardiac Muscle

Muscle tissue with striations and intercalated discs, found in the heart.

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Smooth Muscle

Muscle tissue lacking striations, found in the walls of internal organs.

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Neuron

A nerve cell with a soma, dendrites, and an axon, responsible for transmitting electrical signals.

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Homeostasis

Maintaining a stable internal environment.

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Stimulus (Homeostasis)

The initial event that triggers a homeostatic response.

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Sensor (Homeostasis)

Detects changes or deviations from a set point.

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Integration Center (Homeostasis)

Processes information and determines an appropriate response.

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Effector (Homeostasis)

Produces the output or action to correct the imbalance.

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Visceral Membrane

Outer membrane layer directly on an organ.

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Parietal Membrane

Membrane lining the body cavity wall.

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Main Organs (Examples)

Lungs, heart, liver, stomach, intestines, pancreas, kidneys, spleen.

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Lateral

Toward the side.

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Distal

Away from the point of attachment.

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Proximal

Closer to the point of attachment.

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Frontal (Coronal) Plane

Divides the body into front and back.

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Sagittal Plane

Divides the body into left and right.

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Transverse Plane

Divides the body into upper and lower portions.

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Benedict's Reagent

Tests for glucose.

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Study Notes

Histology Practical - Epithelial Tissue

  • For epithelial cells always identify the free space first
  • Look down to find the cells of interest
  • Simple columnar epithelium has nuclei in a perfect line at the top layer of cells
  • The cells are column-shaped with a single layer of nuclei

Simple Columnar Epithelium

  • Non-ciliated simple columnar epithelium does not have projections
  • This tissue is found in the stomach
  • Just putting simple columnar as the answer to a practical question is acceptable
  • Ciliated simple columnar epithelium has brush-like projections (cilia)
  • Cilia are for movement and movement facilitation
  • This tissue is in the female reproductive tract (uterine tubes)
  • Cilia helps move the oocyte down and sperm up
  • Goblet cells (black lines) secrete mucus for lubrication

Pseudo-stratified Columnar Epithelium

  • The tissue tricks the eye into thinking there's more than one layer
  • Nuclei are squished giving the impression of multiple layers
  • Despite the appearance, it is a single layer
  • Cilia can be seen blowing in the breeze on the surface
  • Trachea (windpipe) uses cilia to trap dust and debris

Simple Squamous Epithelium

  • Difficult to identify looks like Adipose cells
  • The nuclei appear as small dots
  • The cells are drawn out
  • This tissue is found in the lungs
  • Its one layer minimizes the mass of cells in the lungs

Simple Cuboidal Epithelium

  • Has small holes (luminal spaces)
  • Cells directly around the luminal space are of interest
  • Found in the kidney
  • Fluid is filtered through the luminal spaces
  • Filtration is more efficient through one layer of cells

Transitional Epithelium

  • Features transitioning cell types
  • Dome-shaped cells are squat and not elongated
  • Deeper cells are elongated
  • Stretching occurs as the bladder expands and contracts gradually over time
  • This tissue type is found in the bladder

Stratified Squamous Epithelium

  • Has multiple layers of epithelial cells
  • Found in the uterus, specifically the endometrial lining
  • Multiple layers provide protection from erosion
  • Cells lost in the space don't matter due to multiple layers
  • Multiple layers are for cases where abrasion erosion occurs

Keratinized Stratified Squamous Epithelium

  • It's skin
  • The top layer is keratin (protective protein)
  • Protection is the key function
  • Protects deeper layers and connective tissue from damage

Connective Tissue - Areolar

  • Wishbone shape is collagen protein
  • Squiggly, wiggly shapes are elastic fibers
  • Found in the dermis of the skin
  • Elastic fibers provide stretchiness (elasticity)
  • Collagen provides toughness

Connective Tissue - Adipose

  • The cell type is adipocytes
  • Small specs are the nuclei of the cells
  • Found around kidneys for protection and support
  • Location includes around the heart as well for protection

Connective Tissue - Reticular

  • Location is the spleen
  • Network of fibers traps and filters
  • Important for filtering
  • Can be found in the lymph nodes

Connective Tissue - Dense Regular

  • Fibers go in the same direction
  • Found in joints
  • Provides unidirectional strength

Connective Tissue - Dense Irregular

  • Fibers go every which way
  • Provides multi-directional strength
  • Found in the dermis

Connective Tissue - Hyaline Cartilage

  • Found around the ends of long bones (e.g., femur to tibia)
  • Prevents bone from contacting bone
  • Erosion can lead to bone-on-bone contact (osteoarthritis)

Connective Tissue - Fibrocartilage

  • Found between the vertebrae
  • Also between parts of the pelvis (pubic symphysis)

Connective Tissue - Elastic Cartilage

  • Has elastic fibers
  • Found in the ears

Connective Tissue - Bone

  • It's compact bone
  • Located in bones
  • Functions to work with muscle for movement
  • Blood vessels come in through the middle
  • Bone is an active tissue that needs to be nourished

Connective Tissue - Blood

  • Lots of red blood cells
  • Cell types:
    • Red blood cells (erythrocytes)
    • White blood cells (leukocytes)
    • Platelets
  • Red blood cells carry oxygen, white blood cells fight infection, and platelets clot blood

Muscle Tissue - Skeletal

  • Obvious striations (markings)
  • Multi-nucleated (many nuclei)
  • Enables movement

Muscle Tissue - Cardiac

  • Has lines (striations)
  • Has inter calculated discs (junctions) going across the cells

Muscle Tissue - Smooth

  • Doesn't have striations
  • Found in organs (stomach, intestines)
  • Involuntarily controlled

Nervous Tissue - Neuron

  • Large purple cell with projections (dendrites and axon)
  • The large purple part is the soma (cell body)

Practical Exam Review

Homeostasis

  • Definition of homeostasis must be known
  • Give examples such as temperature regulation or blood pressure
  • Know the role of each component:
    • Stimulus: trigger
    • Sensor: detects information
    • Integration: brain or spinal cord determines what to do
    • Effector: drives output
    • Response: body sorts itself out

Membranes

  • Name the membranes (visceral and parietal layers) of the heart (pericardium) and lungs
  • Name the organ in a picture
  • Organs to know: lungs, heart, liver, stomach, intestines, pancreas, kidneys, spleen
  • Take pictures of the torso models

General Body Parts and Orientation

  • Eyes, ears, nose, elbow, shoulder
  • Ears are lateral to the nose
  • Hand is distal to the elbow
  • Know proximal, distal, superior, inferior, anterior, posterior

Slices

  • Frontal, sagittal, transverse, oblique
  • Important consideration for practical questions

Food Testing Lab

  • Know what the different indicators test for:
    • Benedict's: glucose
    • Lugol's/iodine: starch
    • Biuret: protein
  • Don't need to know the colors they change

Microscopy

  • Know the basic anatomy of the scope: ocular, objectives, course and fine adjustment, stage, iris diaphragm, light source
  • Know how to calculate total magnification such as 4x * 10 eye piece = 40x magnification
  • Scanning power (4x) gives a wide field of vision to scan the whole slide
  • Higher power (40x) gives greater resolution, more magnification, but the field of view is reduced

Mitosis

  • Name the stage displayed in images of cells in different stages of mitosis:
    • Interphase
    • Prophase
    • Metaphase
    • Anaphase
    • Telophase
    • Cytokinesis

Osmosis and Diffusion

  • Osmosis: movement of water
  • Diffusion: movement of solutes
  • Large molecules (e.g., starch) cannot move across the membrane because they are too big
  • Smaller molecules (e.g., ions) can diffuse through the membrane

Practical Exam Details

  • 78 questions
  • Questions 20-60 (40 questions) are histology images of epithelial and connective tissue (majority of the exam)

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