Cell Biology Chapter Overview
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Questions and Answers

What is the primary function of the nucleus?

  • Production of proteins
  • Storage of genetic material (correct)
  • Cellular respiration
  • Transportation of nutrients
  • What is the role of the nucleolus within the nucleus?

  • Generating energy for the cell
  • Producing ribosomal RNA (rRNA) and assembling ribosomes (correct)
  • Packaging proteins into vesicles
  • Synthesizing messenger RNA (mRNA)
  • What is the function of nuclear pores?

  • Producing enzymes for cellular processes
  • Regulating the flow of molecules between the nucleus and cytoplasm (correct)
  • Synthesizing lipids for cell membranes
  • Storing energy in the form of ATP
  • What is the primary component of chromatin?

    <p>DNA and proteins (D)</p> Signup and view all the answers

    What happens to chromatin during cell division?

    <p>It condenses to form chromosomes (D)</p> Signup and view all the answers

    What is the estimated total length of DNA in a single human cell, if it were stretched out?

    <p>6.5 kilometers (A)</p> Signup and view all the answers

    What is the MOST LIKELY reason why the nucleus is considered the most conspicuous organelle?

    <p>It is the largest organelle in the cell (B)</p> Signup and view all the answers

    Based on the provided information, what can you conclude about the structure of the nuclear envelope?

    <p>It is composed of two lipid bilayers (D)</p> Signup and view all the answers

    Which of the following is NOT a component of the endomembrane system?

    <p>Mitochondria (C)</p> Signup and view all the answers

    What is the main difference between rough endoplasmic reticulum (RER) and smooth endoplasmic reticulum (SER)?

    <p>RER is studded with ribosomes, while SER lacks ribosomes. (D)</p> Signup and view all the answers

    Where are proteins synthesized that are destined for secretion from a cell?

    <p>Rough endoplasmic reticulum (C)</p> Signup and view all the answers

    Which of the following best describes the transfer of proteins from the endoplasmic reticulum (ER) to the Golgi apparatus?

    <p>Transport within membrane-bound vesicles. (A)</p> Signup and view all the answers

    What is a primary function of the Golgi apparatus?

    <p>Modification and packaging of proteins. (C)</p> Signup and view all the answers

    Which of the following is NOT a function of the smooth endoplasmic reticulum (SER)?

    <p>Protein synthesis. (C)</p> Signup and view all the answers

    Which of these organelles is primarily responsible for the breakdown and recycling of cellular waste products?

    <p>Lysosomes (A)</p> Signup and view all the answers

    The endomembrane system is described as being interconnected. What does this mean?

    <p>Organelles can exchange materials through direct physical connections or vesicles. (D)</p> Signup and view all the answers

    Which of the following statements accurately describes the endosymbiotic theory of the origin of mitochondria and chloroplasts?

    <p>Both mitochondria and chloroplasts originated from prokaryotic cells that were engulfed by eukaryotic cells. (D)</p> Signup and view all the answers

    What evidence supports the idea that mitochondria and chloroplasts evolved from prokaryotes?

    <p>They reproduce independently of the host cell through binary fission. (A)</p> Signup and view all the answers

    Which of the following is NOT a feature shared by mitochondria and chloroplasts?

    <p>Contain an internal membrane system called thylakoids. (D)</p> Signup and view all the answers

    Which of the following best describes the symbiotic relationship between the host cell and the engulfed prokaryotic cell in the endosymbiotic theory?

    <p>The host cell benefits, while the engulfed prokaryotic cell also benefits. (A)</p> Signup and view all the answers

    What is the role of the intermembrane space in mitochondria?

    <p>It plays a role in the production of ATP during oxidative phosphorylation. (B)</p> Signup and view all the answers

    The mitochondrial matrix is the location of which of the following processes?

    <p>Cellular respiration. (A)</p> Signup and view all the answers

    What is the function of the thylakoid membrane in chloroplasts?

    <p>It houses pigments that capture light energy. (C)</p> Signup and view all the answers

    Peroxisomes are single membrane-bound organelles that are involved in various metabolic processes. Which of the following is NOT a function of peroxisomes?

    <p>Production of ATP. (D)</p> Signup and view all the answers

    What is the key function of the signal recognition particle (SRP) in protein synthesis?

    <p>SRP facilitates the insertion of proteins into the endoplasmic reticulum (ER) membrane. (B)</p> Signup and view all the answers

    Which of the following processes is NOT directly involved in insulin synthesis?

    <p>Glycosylation of proinsulin in the Golgi apparatus. (A)</p> Signup and view all the answers

    What is the primary function of the Golgi apparatus in the process of insulin secretion?

    <p>The Golgi apparatus processes and packages insulin into secretory vesicles. (B)</p> Signup and view all the answers

    What is a likely consequence of a defective SRP in a pancreatic beta cell?

    <p>Alteration in the glucose levels in the bloodstream. (A)</p> Signup and view all the answers

    Which organelle is responsible for breaking down damaged or unneeded macromolecules, such as proteins, nucleic acids, lipids, and carbohydrates?

    <p>Lysosome (A)</p> Signup and view all the answers

    What is a primary function of the endoplasmic reticulum (ER) that is crucial for the production of proteins like insulin?

    <p>Synthesis of lipids and proteins (B)</p> Signup and view all the answers

    Which of the following cellular processes involves the fusion of a secretory vesicle with the cell membrane, releasing its contents to the exterior?

    <p>Exocytosis (C)</p> Signup and view all the answers

    Which of the following is NOT a function of the Golgi apparatus?

    <p>Directing ribosomes to the ER membrane (A)</p> Signup and view all the answers

    Which of the following is NOT an organelle found in eukaryotic cells?

    <p>Ribosomes (B)</p> Signup and view all the answers

    What is the primary function of the endoplasmic reticulum (ER)?

    <p>Protein synthesis and modification (A)</p> Signup and view all the answers

    Which organelle is responsible for the breakdown of cellular waste products and damaged organelles?

    <p>Lysosomes (D)</p> Signup and view all the answers

    What is the main function of the nucleus in a eukaryotic cell?

    <p>Storage of genetic information (A)</p> Signup and view all the answers

    Which of the following structures is NOT found in both animal and plant cells?

    <p>Cell wall (C)</p> Signup and view all the answers

    Which of the following statements is TRUE about the cytoskeleton?

    <p>It is involved in cellular movement and structural support (A)</p> Signup and view all the answers

    What is the primary difference between rough ER and smooth ER?

    <p>Rough ER has ribosomes attached to its surface, while smooth ER does not. (C)</p> Signup and view all the answers

    Where are ribosomes made in eukaryotic cells?

    <p>Nucleolus (C)</p> Signup and view all the answers

    Which of the following is NOT a function of ribosomes?

    <p>Transporting proteins to the Golgi apparatus (B)</p> Signup and view all the answers

    What type of signal sequence directs a protein to the peroxisome?

    <p>Ser-Lys-Leu- (B)</p> Signup and view all the answers

    What happens to a protein if its nuclear localization signal (NLS) is altered?

    <p>The protein will remain in the cytoplasm. (B)</p> Signup and view all the answers

    What is the role of a signal peptide in protein sorting?

    <p>To direct proteins to specific organelles. (C)</p> Signup and view all the answers

    How do proteins synthesized by free ribosomes differ from those synthesized by ribosomes attached to the ER?

    <p>Free ribosomes synthesize proteins destined for the cytoplasm, while ER-bound ribosomes synthesize proteins destined for secretion. (D)</p> Signup and view all the answers

    Which of the following is NOT a component of a ribosome?

    <p>mRNA (C)</p> Signup and view all the answers

    What is the difference between the protein A and protein B signals shown in the content?

    <p>Protein A directs the protein to the nucleus while Protein B directs the protein to the peroxisome. (D)</p> Signup and view all the answers

    Flashcards

    Eukaryotic Cell

    A cell with internal membranes forming organelles, specialized for various functions.

    Nucleus

    The organelle that contains the cell's genetic material (DNA).

    Endomembrane System

    A series of membranes in eukaryotic cells that divide the cell into compartments.

    Rough Endoplasmic Reticulum (RER)

    A type of ER studded with ribosomes, involved in protein synthesis.

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    Smooth Endoplasmic Reticulum (SER)

    A type of ER that lacks ribosomes and is involved in lipid synthesis and detoxification.

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    Golgi Apparatus

    An organelle that modifies, sorts, and packages proteins and lipids for secretion or delivery.

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    Mitochondria

    Organelles known as the powerhouses of the cell, generating ATP through respiration.

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    Chloroplasts

    Organelles found in plant cells that convert light energy into chemical energy through photosynthesis.

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    Nuclear Envelope

    The boundary of the nucleus consisting of two lipid bilayer membranes.

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    Nuclear Pores

    Gateways in the nuclear envelope that control molecule traffic in and out of the nucleus.

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    Chromatin

    The complex of DNA and proteins, like histones, that make up chromosomes in non-dividing cells.

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    Chromosome

    Condensed form of chromatin that occurs during cell division, organizing DNA into distinct units.

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    Histone Proteins

    Proteins around which DNA is wrapped, helping to organize chromatin.

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    DNA Length in Cells

    If laid end to end, DNA in a single human cell measures about 6.5 feet to kilometers of DNA total in body.

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    Ribosomes

    Cellular structures that synthesize proteins from amino acids.

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    Protein synthesis

    The process in which cells assemble proteins from amino acids using ribosomes.

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    Signal peptide

    A short amino acid sequence that directs proteins to their proper destination.

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    Nuclear localization signal (NLS)

    A sequence that directs proteins into the nucleus.

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    Free ribosomes

    Ribosomes floating in the cytosol synthesizing proteins that usually stay within the cell.

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    Rough ER

    Endoplasmic reticulum with ribosomes on its surface, synthesizing proteins for secretion or membrane integration.

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    Secretory vesicles

    Vesicles that transport proteins from the Golgi apparatus to the plasma membrane for secretion.

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    Proteins' fates based on synthesis location

    Proteins' destination is determined by whether they are made by free ribosomes or rough ER ribosomes.

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    Components of Endomembrane System

    Includes nuclear envelope, ER, Golgi apparatus, lysosomes, vacuoles, and plasma membrane.

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    Endoplasmic Reticulum (ER)

    A network of membranous tubules; can be rough (with ribosomes) or smooth (without ribosomes).

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    Smooth ER

    ER without ribosomes, involved in lipid synthesis, detoxification, and calcium storage.

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    Vesicles

    Small membrane-bound sacs that transport substances within the endomembrane system.

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    Insulin

    A peptide hormone produced by pancreas beta cells that regulates glucose levels in the body.

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    Proinsulin

    The precursor to insulin, formed by cleaving the signal peptide from preproinsulin.

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    Exocytosis

    The process by which secretory vesicles release their contents outside the cell.

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    Lysosome

    Specialized vesicles full of hydrolytic enzymes that degrade damaged macromolecules.

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    Hydrolysis

    The process of breaking down polymers by adding water to their bonds.

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    Protein Modification

    The process where proteins are altered, often involving glycosylation or lipidation.

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    Intermembrane Space

    The narrow region between the inner and outer membranes of mitochondria.

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    Mitochondrial Matrix

    The innermost space of mitochondria, containing enzymes, DNA, and ribosomes.

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    Thylakoid

    Membrane-bound compartments in chloroplasts arranged in stacks called grana, essential for photosynthesis.

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    Stroma

    The inner space of chloroplasts that contains enzymes, DNA, and ribosomes.

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    Endosymbiosis Theory

    The theory that mitochondria and chloroplasts originated from ingested bacteria that formed symbiotic relationships with cells.

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    Evidence of Endosymbiosis

    Mitochondria and chloroplasts are similar in size to bacteria and have double membranes, free ribosomes, and circular DNA.

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    Peroxisomes

    Specialized metabolic compartments in cells, bounded by a single membrane, performing various reactions.

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    Binary Fission

    A method of asexual reproduction in which a cell divides into two parts, used by bacteria and organelles like mitochondria.

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    Study Notes

    Biology 1 - Cells, Molecular Biology and Genetics (Biol 1000)

    • Course instructor: Dr. Michael Cardinal-Aucoin
    • Course dates: Winter 2025

    The Eukaryotic Cell

    • Eukaryotic cells have internal membranes dividing the cell into compartments called organelles.
    • Each organelle is specialized for a specific function.
    • Plant, animal, and protist cells have most of the same organelles.
    • Organelles include: nucleus, endomembrane system (endoplasmic reticulum, Golgi apparatus, lysosomes, vacuoles), mitochondria, chloroplasts, peroxisomes, and cytoskeleton.

    Eukaryotes

    • Eukaryotic cells have internal membranes to divide the cell into organelles.
    • Organelles have specialized functions.
    • Plant, animal, and protist cells have similar organelles.

    Nucleus

    • Most conspicuous organelle in eukaryotic cells.
    • Stores the DNA.
    • Nuclear envelope: defines the boundary of the nucleus, consists of two lipid bilayer membranes with associated proteins.
    • Nuclear pores: act as gateways allowing molecules to enter and leave the nucleus.
    • Nucleolus: appears as a mass of densely stained granules and fibers. Site where rRNA is synthesized and assembly site of large and small ribosomal subunits.
    • Chromatin: organized DNA molecules associated with proteins (histones and others).
    • Chromosomes: condensed chromatin during cell division.

    Nucleus - Continued

    • DNA in the nucleus is organized as chromatin associated with proteins.
    • Chromatin condenses to form chromosomes during cell division.
    • The DNA in a single human cell stretched end-to-end would measure approximately 6.5 kilometers.

    Nuclear Pore

    • Multiprotein complex that controls traffic into and out of the nucleus.
    • Certain molecules (proteins, building blocks of DNA and RNA, ribosomes, mRNA) travel into or out of the nucleus.
    • Proteins destined for the nucleus have a nuclear localization signal.

    Ribosomes

    • Present in both prokaryotes and eukaryotes.
    • In eukaryotes made in the nucleolus.
    • Complexes made of rRNA and proteins.
    • Sites of protein synthesis where amino acids are assembled into polypeptides.

    Protein Synthesis

    • Proteins synthesized by free ribosomes and those attached to the rough ER have different fates.
    • Free ribosomes in the cytosol produce proteins for use inside the cell.
    • Membrane-bound ribosomes on the rough ER produce proteins for secretion from the cell or for use in lysosomes, vacuoles, and other organelles. (Proteins synthesized by free ribosomes stay in the cytosol)

    How do proteins end up where they should?

    • Proteins possess a signal peptide which acts as a signal sequence directing the protein to the correct organelle.

    Endomembrane System

    • Composed of internal membranes that communicate through physical connections or via transfer vesicles.
    • Components: nuclear envelope, endoplasmic reticulum, Golgi apparatus, lysosomes, vacuoles, and plasma membrane.

    Endoplasmic Reticulum

    • Continuous with the nuclear envelope.
    • Accounts for more than half of the total membrane in eukaryotic cells.
    • Network of membranous tubules and cisternae.
    • Two distinct regions: rough ER and smooth ER.

    Endoplasmic Reticulum (continued)

    • Rough ER: studded with ribosomes. Synthesizes proteins that are secreted, or destined for other organelles (lysosomes, vacuoles etc.) also synthesizes membrane components.
    • Smooth ER: lacks ribosomes. Functions in diverse metabolic processes (e.g., synthesis of lipids, metabolism of carbohydrates, detoxification of drugs/poisons, storage of calcium ions).

    Golgi Apparatus

    • Looks like a series of flattened membrane sacs (cisternae) surrounded by small vesicles.
    • Not physically continuous with the ER.
    • Next stop for most vesicles budding from the ER.
    • Three main roles: modifying lipids and proteins made by the ER, synthesis of carbohydrates, acting as a sorting station (routing proteins to their various destinations).

    Lysosomes

    • Specialized vesicles derived from the Golgi body filled with hydrolytic enzymes.
    • Break down damaged, unneeded macromolecules (e.g., proteins, nucleic acids, lipids, complex carbs).
    • Break down polymers by hydrolysis (chemical reaction to break bonds).
    • Contain proton pumps that maintain an acidic pH for the hydrolytic enzymes to function.
    • Only found in animal cells.

    Lysosomes (continued)

    • Can digest material ingested by cells via phagocytosis.
    • Break down damaged or defective organelles via autophagy.
    • Recycling via lysosomes: receptor-mediated endocytosis, phagocytosis, and autophagy are all part of recycling via the lysosome.

    Vacuoles

    • Large vesicles derived from the endoplasmic reticulum and Golgi apparatus.
    • Perform various functions
    • Food vacuoles store nutrients.
    • Digestive vacuoles act like lysosomes.
    • Contractile vacuoles (in many protists) expel water.
    • Central vacuoles (in many plant cells) regulate turgor pressure in the cell.

    Mitochondria

    • Sites of cellular respiration, a metabolic process that uses oxygen to generate ATP by extracting energy from sugars, fats, and other fuels.
    • Have a single or multiple large structures.
    • Number of mitochondria depends on the cell's function.
    • Enclosed by two membranes: outer smooth membrane, convoluted inner membrane with folds called cristae; inner membrane divides the mitochondrion into intermembrane space and matrix; matrix contains enzymes, mitochondrial DNA, and ribosomes.

    Chloroplasts

    • Capture the energy of sunlight to synthesize sugars (photosynthesis).
    • Surrounded by a double membrane.
    • Contains internal membrane-bound compartments (thylakoids) arranged in stacks called grana.
    • Thylakoid membrane houses pigments that capture energy from light.
    • Stroma (inner-most space) contains enzymes, chloroplast DNA, and ribosomes.

    Origins of Mitochondria and Chloroplasts (Endosymbiosis Theory)

    • Mitochondria may have evolved from ingested aerobic bacteria.
    • Chloroplasts may have evolved from ingested cyanobacteria.
    • Both benefited, forming a symbiotic relationship that became inseparable.

    Evidence supporting Endosymbiosis Theory

    • Similar size to bacteria.
    • Enveloped by a double membrane (ancient phagocytic event).
    • Contain free ribosomes similar to bacterial type and circular DNA molecules.
    • Grow and reproduce independently of the host cell via binary fission (similar to bacteria).

    Peroxisomes

    • Specialized metabolic compartments bounded by a single membrane.
    • Perform various reactions, including breakdown of fatty acids and reduction of reactive oxygen species (creating hydrogen peroxide).
    • Enzymes convert hydrogen peroxide to water and oxygen (catalase).
    • Peroxisomes relation to other organelles remains uncertain.

    Endomembrane System - Exceptions and Summary

    • Mitochondria and chloroplasts are not part of the endomembrane system.
    • The endomembrane system includes the nuclear envelope, ER, Golgi, lysosome, vacuoles, and plasma membrane.

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    This quiz covers key concepts related to the nucleus and endomembrane system in cell biology. Explore questions about the structure and function of these organelles, including the role of chromatin and organelles like the Golgi apparatus. Test your understanding of cellular organization and the synthesis of proteins.

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