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Questions and Answers
What is the primary function of the nucleus?
What is the primary function of the nucleus?
What is the role of the nucleolus within the nucleus?
What is the role of the nucleolus within the nucleus?
What is the function of nuclear pores?
What is the function of nuclear pores?
What is the primary component of chromatin?
What is the primary component of chromatin?
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What happens to chromatin during cell division?
What happens to chromatin during cell division?
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What is the estimated total length of DNA in a single human cell, if it were stretched out?
What is the estimated total length of DNA in a single human cell, if it were stretched out?
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What is the MOST LIKELY reason why the nucleus is considered the most conspicuous organelle?
What is the MOST LIKELY reason why the nucleus is considered the most conspicuous organelle?
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Based on the provided information, what can you conclude about the structure of the nuclear envelope?
Based on the provided information, what can you conclude about the structure of the nuclear envelope?
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Which of the following is NOT a component of the endomembrane system?
Which of the following is NOT a component of the endomembrane system?
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What is the main difference between rough endoplasmic reticulum (RER) and smooth endoplasmic reticulum (SER)?
What is the main difference between rough endoplasmic reticulum (RER) and smooth endoplasmic reticulum (SER)?
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Where are proteins synthesized that are destined for secretion from a cell?
Where are proteins synthesized that are destined for secretion from a cell?
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Which of the following best describes the transfer of proteins from the endoplasmic reticulum (ER) to the Golgi apparatus?
Which of the following best describes the transfer of proteins from the endoplasmic reticulum (ER) to the Golgi apparatus?
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What is a primary function of the Golgi apparatus?
What is a primary function of the Golgi apparatus?
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Which of the following is NOT a function of the smooth endoplasmic reticulum (SER)?
Which of the following is NOT a function of the smooth endoplasmic reticulum (SER)?
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Which of these organelles is primarily responsible for the breakdown and recycling of cellular waste products?
Which of these organelles is primarily responsible for the breakdown and recycling of cellular waste products?
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The endomembrane system is described as being interconnected. What does this mean?
The endomembrane system is described as being interconnected. What does this mean?
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Which of the following statements accurately describes the endosymbiotic theory of the origin of mitochondria and chloroplasts?
Which of the following statements accurately describes the endosymbiotic theory of the origin of mitochondria and chloroplasts?
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What evidence supports the idea that mitochondria and chloroplasts evolved from prokaryotes?
What evidence supports the idea that mitochondria and chloroplasts evolved from prokaryotes?
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Which of the following is NOT a feature shared by mitochondria and chloroplasts?
Which of the following is NOT a feature shared by mitochondria and chloroplasts?
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Which of the following best describes the symbiotic relationship between the host cell and the engulfed prokaryotic cell in the endosymbiotic theory?
Which of the following best describes the symbiotic relationship between the host cell and the engulfed prokaryotic cell in the endosymbiotic theory?
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What is the role of the intermembrane space in mitochondria?
What is the role of the intermembrane space in mitochondria?
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The mitochondrial matrix is the location of which of the following processes?
The mitochondrial matrix is the location of which of the following processes?
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What is the function of the thylakoid membrane in chloroplasts?
What is the function of the thylakoid membrane in chloroplasts?
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Peroxisomes are single membrane-bound organelles that are involved in various metabolic processes. Which of the following is NOT a function of peroxisomes?
Peroxisomes are single membrane-bound organelles that are involved in various metabolic processes. Which of the following is NOT a function of peroxisomes?
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What is the key function of the signal recognition particle (SRP) in protein synthesis?
What is the key function of the signal recognition particle (SRP) in protein synthesis?
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Which of the following processes is NOT directly involved in insulin synthesis?
Which of the following processes is NOT directly involved in insulin synthesis?
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What is the primary function of the Golgi apparatus in the process of insulin secretion?
What is the primary function of the Golgi apparatus in the process of insulin secretion?
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What is a likely consequence of a defective SRP in a pancreatic beta cell?
What is a likely consequence of a defective SRP in a pancreatic beta cell?
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Which organelle is responsible for breaking down damaged or unneeded macromolecules, such as proteins, nucleic acids, lipids, and carbohydrates?
Which organelle is responsible for breaking down damaged or unneeded macromolecules, such as proteins, nucleic acids, lipids, and carbohydrates?
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What is a primary function of the endoplasmic reticulum (ER) that is crucial for the production of proteins like insulin?
What is a primary function of the endoplasmic reticulum (ER) that is crucial for the production of proteins like insulin?
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Which of the following cellular processes involves the fusion of a secretory vesicle with the cell membrane, releasing its contents to the exterior?
Which of the following cellular processes involves the fusion of a secretory vesicle with the cell membrane, releasing its contents to the exterior?
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Which of the following is NOT a function of the Golgi apparatus?
Which of the following is NOT a function of the Golgi apparatus?
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Which of the following is NOT an organelle found in eukaryotic cells?
Which of the following is NOT an organelle found in eukaryotic cells?
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What is the primary function of the endoplasmic reticulum (ER)?
What is the primary function of the endoplasmic reticulum (ER)?
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Which organelle is responsible for the breakdown of cellular waste products and damaged organelles?
Which organelle is responsible for the breakdown of cellular waste products and damaged organelles?
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What is the main function of the nucleus in a eukaryotic cell?
What is the main function of the nucleus in a eukaryotic cell?
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Which of the following structures is NOT found in both animal and plant cells?
Which of the following structures is NOT found in both animal and plant cells?
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Which of the following statements is TRUE about the cytoskeleton?
Which of the following statements is TRUE about the cytoskeleton?
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What is the primary difference between rough ER and smooth ER?
What is the primary difference between rough ER and smooth ER?
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Where are ribosomes made in eukaryotic cells?
Where are ribosomes made in eukaryotic cells?
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Which of the following is NOT a function of ribosomes?
Which of the following is NOT a function of ribosomes?
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What type of signal sequence directs a protein to the peroxisome?
What type of signal sequence directs a protein to the peroxisome?
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What happens to a protein if its nuclear localization signal (NLS) is altered?
What happens to a protein if its nuclear localization signal (NLS) is altered?
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What is the role of a signal peptide in protein sorting?
What is the role of a signal peptide in protein sorting?
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How do proteins synthesized by free ribosomes differ from those synthesized by ribosomes attached to the ER?
How do proteins synthesized by free ribosomes differ from those synthesized by ribosomes attached to the ER?
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Which of the following is NOT a component of a ribosome?
Which of the following is NOT a component of a ribosome?
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What is the difference between the protein A and protein B signals shown in the content?
What is the difference between the protein A and protein B signals shown in the content?
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Flashcards
Eukaryotic Cell
Eukaryotic Cell
A cell with internal membranes forming organelles, specialized for various functions.
Nucleus
Nucleus
The organelle that contains the cell's genetic material (DNA).
Endomembrane System
Endomembrane System
A series of membranes in eukaryotic cells that divide the cell into compartments.
Rough Endoplasmic Reticulum (RER)
Rough Endoplasmic Reticulum (RER)
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Smooth Endoplasmic Reticulum (SER)
Smooth Endoplasmic Reticulum (SER)
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Golgi Apparatus
Golgi Apparatus
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Mitochondria
Mitochondria
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Chloroplasts
Chloroplasts
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Nuclear Envelope
Nuclear Envelope
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Nuclear Pores
Nuclear Pores
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Chromatin
Chromatin
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Chromosome
Chromosome
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Histone Proteins
Histone Proteins
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DNA Length in Cells
DNA Length in Cells
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Ribosomes
Ribosomes
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Protein synthesis
Protein synthesis
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Signal peptide
Signal peptide
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Nuclear localization signal (NLS)
Nuclear localization signal (NLS)
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Free ribosomes
Free ribosomes
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Rough ER
Rough ER
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Secretory vesicles
Secretory vesicles
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Proteins' fates based on synthesis location
Proteins' fates based on synthesis location
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Components of Endomembrane System
Components of Endomembrane System
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Endoplasmic Reticulum (ER)
Endoplasmic Reticulum (ER)
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Smooth ER
Smooth ER
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Vesicles
Vesicles
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Insulin
Insulin
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Proinsulin
Proinsulin
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Exocytosis
Exocytosis
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Lysosome
Lysosome
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Hydrolysis
Hydrolysis
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Protein Modification
Protein Modification
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Intermembrane Space
Intermembrane Space
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Mitochondrial Matrix
Mitochondrial Matrix
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Thylakoid
Thylakoid
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Stroma
Stroma
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Endosymbiosis Theory
Endosymbiosis Theory
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Evidence of Endosymbiosis
Evidence of Endosymbiosis
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Peroxisomes
Peroxisomes
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Binary Fission
Binary Fission
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Study Notes
Biology 1 - Cells, Molecular Biology and Genetics (Biol 1000)
- Course instructor: Dr. Michael Cardinal-Aucoin
- Course dates: Winter 2025
The Eukaryotic Cell
- Eukaryotic cells have internal membranes dividing the cell into compartments called organelles.
- Each organelle is specialized for a specific function.
- Plant, animal, and protist cells have most of the same organelles.
- Organelles include: nucleus, endomembrane system (endoplasmic reticulum, Golgi apparatus, lysosomes, vacuoles), mitochondria, chloroplasts, peroxisomes, and cytoskeleton.
Eukaryotes
- Eukaryotic cells have internal membranes to divide the cell into organelles.
- Organelles have specialized functions.
- Plant, animal, and protist cells have similar organelles.
Nucleus
- Most conspicuous organelle in eukaryotic cells.
- Stores the DNA.
- Nuclear envelope: defines the boundary of the nucleus, consists of two lipid bilayer membranes with associated proteins.
- Nuclear pores: act as gateways allowing molecules to enter and leave the nucleus.
- Nucleolus: appears as a mass of densely stained granules and fibers. Site where rRNA is synthesized and assembly site of large and small ribosomal subunits.
- Chromatin: organized DNA molecules associated with proteins (histones and others).
- Chromosomes: condensed chromatin during cell division.
Nucleus - Continued
- DNA in the nucleus is organized as chromatin associated with proteins.
- Chromatin condenses to form chromosomes during cell division.
- The DNA in a single human cell stretched end-to-end would measure approximately 6.5 kilometers.
Nuclear Pore
- Multiprotein complex that controls traffic into and out of the nucleus.
- Certain molecules (proteins, building blocks of DNA and RNA, ribosomes, mRNA) travel into or out of the nucleus.
- Proteins destined for the nucleus have a nuclear localization signal.
Ribosomes
- Present in both prokaryotes and eukaryotes.
- In eukaryotes made in the nucleolus.
- Complexes made of rRNA and proteins.
- Sites of protein synthesis where amino acids are assembled into polypeptides.
Protein Synthesis
- Proteins synthesized by free ribosomes and those attached to the rough ER have different fates.
- Free ribosomes in the cytosol produce proteins for use inside the cell.
- Membrane-bound ribosomes on the rough ER produce proteins for secretion from the cell or for use in lysosomes, vacuoles, and other organelles. (Proteins synthesized by free ribosomes stay in the cytosol)
How do proteins end up where they should?
- Proteins possess a signal peptide which acts as a signal sequence directing the protein to the correct organelle.
Endomembrane System
- Composed of internal membranes that communicate through physical connections or via transfer vesicles.
- Components: nuclear envelope, endoplasmic reticulum, Golgi apparatus, lysosomes, vacuoles, and plasma membrane.
Endoplasmic Reticulum
- Continuous with the nuclear envelope.
- Accounts for more than half of the total membrane in eukaryotic cells.
- Network of membranous tubules and cisternae.
- Two distinct regions: rough ER and smooth ER.
Endoplasmic Reticulum (continued)
- Rough ER: studded with ribosomes. Synthesizes proteins that are secreted, or destined for other organelles (lysosomes, vacuoles etc.) also synthesizes membrane components.
- Smooth ER: lacks ribosomes. Functions in diverse metabolic processes (e.g., synthesis of lipids, metabolism of carbohydrates, detoxification of drugs/poisons, storage of calcium ions).
Golgi Apparatus
- Looks like a series of flattened membrane sacs (cisternae) surrounded by small vesicles.
- Not physically continuous with the ER.
- Next stop for most vesicles budding from the ER.
- Three main roles: modifying lipids and proteins made by the ER, synthesis of carbohydrates, acting as a sorting station (routing proteins to their various destinations).
Lysosomes
- Specialized vesicles derived from the Golgi body filled with hydrolytic enzymes.
- Break down damaged, unneeded macromolecules (e.g., proteins, nucleic acids, lipids, complex carbs).
- Break down polymers by hydrolysis (chemical reaction to break bonds).
- Contain proton pumps that maintain an acidic pH for the hydrolytic enzymes to function.
- Only found in animal cells.
Lysosomes (continued)
- Can digest material ingested by cells via phagocytosis.
- Break down damaged or defective organelles via autophagy.
- Recycling via lysosomes: receptor-mediated endocytosis, phagocytosis, and autophagy are all part of recycling via the lysosome.
Vacuoles
- Large vesicles derived from the endoplasmic reticulum and Golgi apparatus.
- Perform various functions
- Food vacuoles store nutrients.
- Digestive vacuoles act like lysosomes.
- Contractile vacuoles (in many protists) expel water.
- Central vacuoles (in many plant cells) regulate turgor pressure in the cell.
Mitochondria
- Sites of cellular respiration, a metabolic process that uses oxygen to generate ATP by extracting energy from sugars, fats, and other fuels.
- Have a single or multiple large structures.
- Number of mitochondria depends on the cell's function.
- Enclosed by two membranes: outer smooth membrane, convoluted inner membrane with folds called cristae; inner membrane divides the mitochondrion into intermembrane space and matrix; matrix contains enzymes, mitochondrial DNA, and ribosomes.
Chloroplasts
- Capture the energy of sunlight to synthesize sugars (photosynthesis).
- Surrounded by a double membrane.
- Contains internal membrane-bound compartments (thylakoids) arranged in stacks called grana.
- Thylakoid membrane houses pigments that capture energy from light.
- Stroma (inner-most space) contains enzymes, chloroplast DNA, and ribosomes.
Origins of Mitochondria and Chloroplasts (Endosymbiosis Theory)
- Mitochondria may have evolved from ingested aerobic bacteria.
- Chloroplasts may have evolved from ingested cyanobacteria.
- Both benefited, forming a symbiotic relationship that became inseparable.
Evidence supporting Endosymbiosis Theory
- Similar size to bacteria.
- Enveloped by a double membrane (ancient phagocytic event).
- Contain free ribosomes similar to bacterial type and circular DNA molecules.
- Grow and reproduce independently of the host cell via binary fission (similar to bacteria).
Peroxisomes
- Specialized metabolic compartments bounded by a single membrane.
- Perform various reactions, including breakdown of fatty acids and reduction of reactive oxygen species (creating hydrogen peroxide).
- Enzymes convert hydrogen peroxide to water and oxygen (catalase).
- Peroxisomes relation to other organelles remains uncertain.
Endomembrane System - Exceptions and Summary
- Mitochondria and chloroplasts are not part of the endomembrane system.
- The endomembrane system includes the nuclear envelope, ER, Golgi, lysosome, vacuoles, and plasma membrane.
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Description
This quiz covers key concepts related to the nucleus and endomembrane system in cell biology. Explore questions about the structure and function of these organelles, including the role of chromatin and organelles like the Golgi apparatus. Test your understanding of cellular organization and the synthesis of proteins.