Cell Biology and Transport Mechanisms
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Questions and Answers

Which of the following actions is permitted under the copyright policy for year-1 course materials at St. George's University School of Medicine?

  • Downloading and sharing 'view only' lecture recordings with colleagues.
  • Distributing modified versions of downloaded files to students not enrolled in the course.
  • Publishing parts of the course material on a personal website without permission.
  • Creating print copies of downloadable files for personal use while maintaining the copyright statement. (correct)
  • Which cellular component is responsible for synthesizing proteins?

  • Lysosome
  • Golgi Complex
  • Ribosome (correct)
  • Endoplasmic Reticulum
  • Which organelle is primarily involved in the modification, sorting, and packaging of proteins and lipids?

  • Golgi Complex (correct)
  • Peroxisome
  • Mitochondria
  • Endoplasmic Reticulum
  • What is the main function of the lysosomes?

    <p>Breaking down cellular waste and debris. (A)</p> Signup and view all the answers

    Which of the following best describes the role of the cytoskeleton?

    <p>Providing structural support and facilitating cell movement. (B)</p> Signup and view all the answers

    What is the primary role of the plasma membrane?

    <p>To regulate the movement of substances into and out of the cell. (D)</p> Signup and view all the answers

    Which transport mechanism moves molecules against their concentration gradient, requiring energy?

    <p>Active Transport (B)</p> Signup and view all the answers

    How does increased membrane fluidity affect the permeability of a cell membrane?

    <p>It increases permeability to small, nonpolar molecules. (D)</p> Signup and view all the answers

    A cell is placed in a solution, and it begins to swell. Which of the following cellular components is primarily responsible for preventing the cell from bursting due to the influx of water?

    <p>Cytoskeleton (D)</p> Signup and view all the answers

    A researcher is studying a cell and observes that it is actively secreting a large protein. Which of the following transport mechanisms is most likely involved in this process?

    <p>Exocytosis (A)</p> Signup and view all the answers

    In a patient with hyperglycemia (high blood glucose), such as the 65-year-old female described, which cellular process is most directly affected, leading to a disruption in cellular homeostasis?

    <p>Glycolysis in the cytosol (C)</p> Signup and view all the answers

    If a toxin disrupts the function of the cytoskeleton, which of the following cellular processes would be most immediately affected?

    <p>Cellular movement and shape (B)</p> Signup and view all the answers

    Which of the following best describes the role of the plasma membrane in maintaining cellular homeostasis?

    <p>Selectively regulating the passage of substances into and out of the cell. (B)</p> Signup and view all the answers

    In the provided scenario of a patient with Type 2 Diabetes, which organelle is most directly involved in the initial response to elevated blood glucose levels through insulin production?

    <p>Ribosome (B)</p> Signup and view all the answers

    Which of the following represents the correct sequence of cellular structures involved in the production and secretion of insulin, starting from the synthesis of the insulin protein?

    <p>Ribosome → Cytosol → Plasma membrane (C)</p> Signup and view all the answers

    A pharmaceutical company is designing a drug that needs to enter cells rapidly. Which strategy would best facilitate the drug's entry across the plasma membrane?

    <p>Formulating the drug with a hydrophobic carrier to enhance diffusion. (B)</p> Signup and view all the answers

    Which of the following characteristics distinguishes microtubules from microfilaments?

    <p>Microtubules are larger in size compared to microfilaments. (B)</p> Signup and view all the answers

    Keratin, neurofilaments, and glial fibrillary acidic protein are the main components of which type of filament?

    <p>Intermediate filaments (B)</p> Signup and view all the answers

    If a cell were unable to produce tubulin, which of the following functions would be most directly affected?

    <p>Chromosome movement during cell division (B)</p> Signup and view all the answers

    Which of these cellular activities does NOT involve microfilaments?

    <p>Movement of cilia and flagella (A)</p> Signup and view all the answers

    Which of the following is a primary function of the cell nucleus?

    <p>Control of cellular activities through gene expression (B)</p> Signup and view all the answers

    What is the role of nuclear pores found in the nuclear envelope?

    <p>To regulate the movement of substances into and out of the nucleus. (C)</p> Signup and view all the answers

    Ribosomal subunits are assembled from rRNA and proteins within which cellular structure?

    <p>Nucleolus (A)</p> Signup and view all the answers

    Which of the following accurately describes the role of genes within a cell?

    <p>Controlling cellular structure and directing cellular activities. (C)</p> Signup and view all the answers

    How does the presence of multiple nucleoli in a cell such as a hepatocyte relate to the cell's function?

    <p>It reflects a high level of ribosome subunit production. (B)</p> Signup and view all the answers

    Mature red blood cells lack a nucleus. How does this affect their function?

    <p>It allows them to carry more oxygen. (B)</p> Signup and view all the answers

    What is the primary function of the cell membrane's lipid bilayer?

    <p>To control the flow of substances into and out of the cell. (C)</p> Signup and view all the answers

    Which of the following is a characteristic feature of integral proteins embedded in the cell membrane?

    <p>Many act as transmembrane proteins, spanning the entire lipid bilayer. (C)</p> Signup and view all the answers

    How does the glycocalyx contribute to the function of a cell?

    <p>By enabling cell recognition, adhesion, and protection. (B)</p> Signup and view all the answers

    Peripheral proteins are associated with the cell membrane in what way?

    <p>They are attached to the polar heads of membrane lipids or integral proteins. (D)</p> Signup and view all the answers

    If a drug were designed to block the function of the glycocalyx, which cellular process would be MOST directly affected?

    <p>Cell-to-cell recognition and adhesion. (D)</p> Signup and view all the answers

    Which of the following is NOT a function of integral proteins?

    <p>Synthesizing lipids for the cell membrane. (B)</p> Signup and view all the answers

    What cellular function is NOT directly facilitated by proteins associated with the plasma membrane?

    <p>Direct synthesis of ATP for cellular energy. (B)</p> Signup and view all the answers

    A cell membrane is permeable to oxygen, carbon dioxide and steroids, moderately permeable to water and urea but impermeable to ions and large uncharged polar molecules. This is an example of:

    <p>Selective permeability. (A)</p> Signup and view all the answers

    Which of the following scenarios best illustrates the concept of an electrochemical gradient?

    <p>The clustering of negatively charged ions near the inner surface of a cell membrane coupled with a higher concentration of these ions outside the cell. (B)</p> Signup and view all the answers

    Cilia and flagella share a common structural element in their core. What is this element, and what is its arrangement?

    <p>Microtubules in a '9+2' arrangement. (B)</p> Signup and view all the answers

    In which of the following locations would you expect to find cells with a high density of microvilli?

    <p>The lining of the small intestine. (A)</p> Signup and view all the answers

    Primary active transport directly utilizes which of the following as an energy source?

    <p>Adenosine triphosphate (ATP). (D)</p> Signup and view all the answers

    Which transport process is characterized by movement down a concentration or electrical gradient?

    <p>Passive transport. (D)</p> Signup and view all the answers

    A researcher observes a cell using energy to transport a substance across its membrane against its concentration gradient. What type of transport is MOST likely occurring?

    <p>Active transport. (D)</p> Signup and view all the answers

    Which of the following transport mechanisms relies directly on ATP hydrolysis to move substances across the cell membrane?

    <p>Primary active transport of sodium ions (B)</p> Signup and view all the answers

    A cell needs to import a large quantity of LDLs (low-density lipoproteins). Which transport mechanism would be MOST efficient for this purpose?

    <p>Receptor-mediated endocytosis (C)</p> Signup and view all the answers

    How do symporters and antiporters facilitate secondary active transport?

    <p>By using the electrochemical gradient of one ion to move another molecule (B)</p> Signup and view all the answers

    Which of the following factors has the LEAST direct impact on the rate of simple diffusion across a plasma membrane?

    <p>Presence of integral membrane proteins (D)</p> Signup and view all the answers

    A macrophage encounters a bacterium. Which process would the macrophage use to engulf and internalize the bacterium?

    <p>Phagocytosis (C)</p> Signup and view all the answers

    Which of the following BEST describes the process of transcytosis?

    <p>The movement of substances into, across, and out of a cell. (A)</p> Signup and view all the answers

    What is a key difference between bulk-phase endocytosis (pinocytosis) and receptor-mediated endocytosis?

    <p>Pinocytosis is non-selective, while receptor-mediated endocytosis is highly selective. (C)</p> Signup and view all the answers

    Which transport process is responsible for the secretion of digestive enzymes by pancreatic cells?

    <p>Exocytosis (C)</p> Signup and view all the answers

    A nerve cell releases neurotransmitters into the synapse. Which transport mechanism is directly involved in this process?

    <p>Exocytosis (A)</p> Signup and view all the answers

    Which of the following substances would MOST likely require facilitated diffusion to cross the cell membrane?

    <p>Glucose (D)</p> Signup and view all the answers

    Flashcards

    Copyright protection

    All year-1 course materials are legally protected from copying and distribution.

    Cytoplasm

    The jelly-like substance inside the cell where organelles are located.

    Cytosol

    The liquid portion of the cytoplasm, which houses organelles and cellular components.

    Organelles

    Specialized structures within a cell, each with specific functions.

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    Plasma membrane

    The boundary of the cell controlling entry and exit of substances.

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    Passive transport

    Movement of substances across cell membranes without energy, like diffusion and osmosis.

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    Active transport

    Movement requiring energy to move substances against their gradient.

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    Electrochemical gradient

    A combination of concentration and electrical gradients affecting ion movement across membranes.

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    Microfilaments

    Thinnest filaments composed of actin, aiding in movement and shape.

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    Intermediate filaments

    Filaments made of keratin that stabilize cell structures and connections.

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    Microtubules

    Large filaments made of tubulin that shape cells and aid in movement.

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    Function of microfilaments

    Generate movement and provide mechanical support and shape to the cell.

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    Function of intermediate filaments

    Stabilize organelle positions and attach cells to one another.

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    Function of microtubules

    Determine cell shape and assist with movement of structures inside the cell.

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    Nucleus

    Prominent structure controlling cellular activities and producing ribosomal subunits.

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    Nuclear envelope

    Double membrane around the nucleus with pores for molecule exchange.

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    Genes

    Hereditary units in the nucleus that control cellular structure and activities, composed of DNA.

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    DNA

    A double helix structure that stores genetic information in cells and is packaged as nucleosomes.

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    Lipid bilayer

    A flexible membrane structure made of two layers of lipids, forming the cell's barrier.

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    Integral proteins

    Proteins that are embedded in the lipid bilayer, many functioning as channels or receptors.

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    Glycocalyx

    A coating formed by glycoproteins and glycolipids that help cells recognize and adhere to each other.

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    Selective permeability

    The ability of the plasma membrane to allow certain substances to pass more easily than others.

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    Peripheral proteins

    Proteins that are loosely attached to the membrane's surface, not firmly embedded.

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    Concentration gradient

    The difference in concentration of a substance across a space.

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    Transmembrane proteins

    Integral proteins that extend across the lipid bilayer, often acting as channels or transporters.

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    Electrical gradient

    The difference in charge across a membrane, where one side is more negatively charged than the other.

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    Cilia

    Hair-like structures that move fluid along cell surfaces, like in the respiratory tract.

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    Flagella

    Long projections that enable entire cells to move, such as sperm cells.

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    Microvilli

    Tiny projections that increase the surface area for absorption in the digestive system.

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    Passive vs Active Transport

    Passive Transport moves substances down gradients without energy; Active Transport moves substances against gradients requiring energy.

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    Secondary active transport

    Transport that indirectly uses ATP via ion gradients, moving substances against their concentration gradient.

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    Facilitated diffusion

    Transport of substances across a membrane through specific carrier proteins, without energy expenditure.

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    Osmosis

    The diffusion of water through a selectively permeable membrane, driven by concentration differences.

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    Vesicle

    A small spherical sac used to transport substances within cells or to release them outside the cell.

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    Endocytosis

    Process by which cells engulf external substances, creating vesicles from the plasma membrane.

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    Phagocytosis

    Type of endocytosis where a cell engulfs large solid particles to protect the body.

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    Receptor-mediated endocytosis

    Selective uptake of specific ligands by cells after binding to receptor proteins on the membrane.

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    Bulk-phase endocytosis

    Type of endocytosis that engulfs tiny droplets of extracellular fluid, often called pinocytosis.

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    Exocytosis

    The process of vesicles releasing their contents outside the cell, such as hormones or neurotransmitters.

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    Transcytosis

    Process of moving substances into, across, and out of a cell, especially in epithelial cells.

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    Plasma membrane role

    Controls communication and substance exchange between cells and their environment.

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    Cytoskeleton

    Network of protein filaments that gives the cell its shape and helps organize contents.

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    Cytosol functions

    Fluid portion of cytoplasm where biochemical reactions occur.

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    Nucleus role

    Controls cell activities and houses the cell's DNA.

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    Study Notes

    • All year-1 course materials, whether printed or online, are protected by copyright.
    • Copying, distributing, or publishing any part of the materials is prohibited.
    • Students and faculty are permitted to make copies for personal and classroom use only, as long as the copyright statement is maintained.
    • "View only" files (e.g., lecture recordings) cannot be copied.

    Objectives

    • Identify and describe the three main parts of a cell.
    • Describe the basic structure and function of the cytoplasm:
      • Cytosol: Describe structure and function of cytoskeleton.
      • Organelles: Describe ribosomes, endoplasmic reticulum, Golgi complex, lysosomes, peroxisomes, proteasomes, and mitochondria.
      • Nucleus: Describe structure and function of the nucleus, including the nuclear envelope, pores, nucleoli, genes, and chromosomes.
    • Describe the composition and function of the plasma cell membrane.
      • List the different components of the plasma membrane (phospholipids, glycoproteins, glycolipids, cholesterol) and describe their functions
      • Explain membrane fluidity, permeability, and how it affects transport
    • Describe the various types of transport across the cell membrane:
      • Passive processes: simple diffusion, facilitated diffusion, osmosis, and osmotic pressure.
      • Active Transport: Differentiate from passive processes and compare types
      • Vesicular transport: endocytosis (3 types), exocytosis, transcytosis
    • Explain the concept of concentration and electrical gradients and their contribution to the electrochemical gradient.
    • Describe cell specializations (cilia, flagella, microvilli).
      • Include typical locations and functions

    Case Study (Patient)

    • A 65-year-old female with a history of Type 2 diabetes mellitus is brought to the emergency department with altered mental status (confused, disoriented), drowsiness, and dry oral mucosa.

    • Blood glucose levels are >600 mg/dL (normal is 70-100 mg/dL).

    • Treatment includes IV fluids, insulin, and electrolyte replacement.

    • Questions about the case:

      • Symptom indicating cell state: What symptom indicated the state of the cells in the patient?
      • Major cause of condition: What is the primary cause of the patient's condition?
      • Cellular barrier: Which cell part acts as a barrier between the cell's contents and its surroundings?
      • Membrane permeability's role: Explain the role of membrane permeability in response to the IV fluids given to the patient.
      • Insulin production's structure: Which organelle is responsible for the instructions for insulin production?
      • Insulin production process: List the cell structures involved in insulin production from synthesis to secretion.

    The Cell's Parts and Function

    • Plasma membrane

    • The outer boundary of the cell

    • Selective barrier; controls the passage of substances into and out of the cell

    • Maintains cellular integrity by isolating the intracellular environment

    • Critical in communication between cells and the extracellular environment

    • Cytoplasm

    • The region within the plasma membrane, excluding the nucleus

    • Contains cytosol, which is an intracellular fluid, and organelles.

    • Nucleus

    • Membrane-bound organelle containing most of the cell's genetic material (DNA).

    • Controls cellular activities and structure by regulating gene expression and protein synthesis

    Cell Structures and Function

    • Organelles (small, specialized components with their own specific structural characteristics and functions):
      • Centrosomes
      • Cilia and Flagella
      • Ribosomes
      • ER(Endoplasmic Reticulum) Types- Rough ER, Smooth ER
      • Golgi Complexes
      • Lysosomes
      • Perozisomes
      • Proteasomes
      • Mitochondria
    • Cytoskeleton: Network of protein filaments (microtubules, intermediate filaments, microfilaments) that provides structural support and facilitates movement of organelles, chromosomes, and cells.

    Membrane Transport

    • Permeable

    • Allows a substance to pass through

    • Impermeable

    • Does not allow a substance to pass through

    • Selective Permeability

    • Allows certain substances to pass more readily than others

    • Various types

    • Simple diffusion

    • Facilitated diffusion

    • Osmosis and osmotic pressure

    • Active transport (primary and secondary)

    • Vesicular transport (endocytosis, exocytosis, transcytosis)

    • Passive and Active Transport

      • Passive transport: Movement of substances across the membrane without energy expenditure (e.g., simple diffusion, facilitated diffusion, osmosis).
      • Active transport: requires energy (ATP) to move substances against their concentration gradients.
    • GRADIENTS

      • Concentration gradients:differences in the concentrations of a molecule across a space.
      • Electrical gradient:differences in electrical charge across a space(membrane potential)
      • Electrochemical gradient:Combined influence of concentration and electrical gradients on the movement of a given molecule or ion.

    Cell Membrane Specializations

    • Cilia and flagella: Motile projections that aid in movement or fluid transport
    • Microvilli: Small projections that increase the surface area for absorption and secretion.

    Cell Membrane Transport Mechanisms

    • Endocytosis: The process of taking substances into the cell through membrane vesicles, including:
      • Receptor-mediated endocytosis
      • Phagocytosis
      • Bulk-phase endocytosis (pinocytosis)
    • Exocytosis: The process of releasing substances out of the cell through membrane vesicles.
    • Transcytosis: The process of moving substances across cells (e.g., epithelial cells).

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    Description

    Test your knowledge of cellular components, protein synthesis, and transport mechanisms. This quiz covers the functions of organelles like lysosomes and the role of the cytoskeleton. It also tests understanding of membrane fluidity and cell response to osmotic changes.

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