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Questions and Answers
What is cell culture?
The removal of cells from an animal, plant, or microorganism and their subsequent growth in a favorable artificial environment.
Define microbial culture.
A method of multiplying microbial organisms by letting them reproduce in predetermined culture medium under controlled laboratory conditions.
What are the primary diagnostic methods of microbiology?
Microbial cultures.
How are microbial cultures used in molecular biology?
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What is the purpose of a microbiological culture?
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Why is a favorable artificial environment important in cell culture?
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What is the primary diagnostic method of microbiology used to determine the cause of infectious disease?
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What type of cells are usually used in animal cell culture?
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What is the causative agent of strep throat that can be screened for using a throat culture?
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What are some of the areas where animal cell culture has found most applications?
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What is the term used informally to refer to 'selectively growing' a specific kind of microorganism in the lab?
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What is the type of biotechnological technique where animal cells are artificially grown in a favorable environment?
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What has enabled the working of a wide variety of cells under different conditions in animal cell culture?
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What can be used to initiate organ culture in vitro for animal cell culture?
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What is more difficult than growing microorganisms on artificial media in animal cell culture?
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What has made it possible to culture both undifferentiated and differentiated cells on artificial media in animal cell culture?
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Study Notes
Cell Culture and Microbiology
- Cell culture is the process of growing cells in a controlled environment, outside of their natural habitat.
- Microbial culture is the growth of microorganisms, such as bacteria, viruses, and fungi, in a controlled environment.
Primary Diagnostic Methods of Microbiology
- Primary diagnostic methods of microbiology include:
- Microbial culture: used to determine the cause of infectious disease
- Microscopy: used to visualize microorganisms
- Biochemical tests: used to identify microorganisms
Microbial Cultures in Molecular Biology
- Microbial cultures are used in molecular biology to:
- Isolate and study microorganisms
- Produce recombinant DNA and proteins
- Develop vaccines and diagnostic tools
Purpose of Microbiological Culture
- The purpose of microbiological culture is to:
- Isolate and identify microorganisms
- Determine the cause of infectious disease
- Develop vaccines and diagnostic tools
Importance of Artificial Environment in Cell Culture
- A favorable artificial environment is important in cell culture because it:
- Supports the growth and survival of cells
- Allows for the control of experimental conditions
- Enables the study of cellular processes and responses
Primary Diagnostic Method of Microbiology
- The primary diagnostic method of microbiology used to determine the cause of infectious disease is microbial culture.
Animal Cell Culture
- Animal cell culture typically involves the use of:
- Immortalized cells or primary cells
- Cells derived from animal tissues, such as skin or muscle
Causative Agent of Strep Throat
- The causative agent of strep throat that can be screened for using a throat culture is Group A beta-hemolytic streptococcus (GABHS).
Applications of Animal Cell Culture
- Animal cell culture has found most applications in:
- Biotechnology and biopharmaceutical industries
- Cancer research and therapy
- Tissue engineering and regenerative medicine
Selective Growth of Microorganisms
- The term used informally to refer to 'selectively growing' a specific kind of microorganism in the lab is "selective culture".
Animal Cell Culture Technique
- The type of biotechnological technique where animal cells are artificially grown in a favorable environment is cell culture.
Enabling Technology in Animal Cell Culture
- The development of serum-free media has enabled the working of a wide variety of cells under different conditions in animal cell culture.
Initiation of Organ Culture
- Organ culture can be initiated in vitro for animal cell culture using:
- Tissue fragments or explants
- Dissociated cells
Difficulty in Animal Cell Culture
- Growing microorganisms on artificial media is generally easier than growing animal cells, because animal cells require more complex and defined media.
Culture of Undifferentiated and Differentiated Cells
- Advances in cell culture media and technology have made it possible to culture both undifferentiated and differentiated cells on artificial media in animal cell culture.
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Description
Test your knowledge about cell and microbial culture with this quiz. Learn about the growth of cells in culture and the factors that affect their growth, as well as the method of multiplying microbial organisms in predetermined culture medium under controlled laboratory conditions.
