Cell and Microbial Culture

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Questions and Answers

What is cell culture?

The removal of cells from an animal, plant, or microorganism and their subsequent growth in a favorable artificial environment.

Define microbial culture.

A method of multiplying microbial organisms by letting them reproduce in predetermined culture medium under controlled laboratory conditions.

What are the primary diagnostic methods of microbiology?

Microbial cultures.

How are microbial cultures used in molecular biology?

As research tools. Signup and view all the answers

What is the purpose of a microbiological culture?

To determine the type of organism and its abundance in the sample being tested. Signup and view all the answers

Why is a favorable artificial environment important in cell culture?

To facilitate the growth of cells outside of their natural habitat. Signup and view all the answers

What is the primary diagnostic method of microbiology used to determine the cause of infectious disease?

Microbial cultures Signup and view all the answers

What type of cells are usually used in animal cell culture?

Multicellular eukaryotic cells Signup and view all the answers

What is the causative agent of strep throat that can be screened for using a throat culture?

Streptococcus pyogenes Signup and view all the answers

What are some of the areas where animal cell culture has found most applications?

Cancer research, vaccine production, and gene therapy Signup and view all the answers

What is the term used informally to refer to 'selectively growing' a specific kind of microorganism in the lab?

Culture Signup and view all the answers

What is the type of biotechnological technique where animal cells are artificially grown in a favorable environment?

Animal cell culture Signup and view all the answers

What has enabled the working of a wide variety of cells under different conditions in animal cell culture?

Development of basic tissue culture media Signup and view all the answers

What can be used to initiate organ culture in vitro for animal cell culture?

Complex structures like organs Signup and view all the answers

What is more difficult than growing microorganisms on artificial media in animal cell culture?

The growth of animal cells Signup and view all the answers

What has made it possible to culture both undifferentiated and differentiated cells on artificial media in animal cell culture?

Advances in the culture media Signup and view all the answers

Study Notes

Cell Culture and Microbiology

Cell culture is the process of growing cells in a controlled environment, outside of their natural habitat.
Microbial culture is the growth of microorganisms, such as bacteria, viruses, and fungi, in a controlled environment.

Primary Diagnostic Methods of Microbiology

Primary diagnostic methods of microbiology include:
- Microbial culture: used to determine the cause of infectious disease
- Microscopy: used to visualize microorganisms
- Biochemical tests: used to identify microorganisms

Microbial Cultures in Molecular Biology

Microbial cultures are used in molecular biology to:
- Isolate and study microorganisms
- Produce recombinant DNA and proteins
- Develop vaccines and diagnostic tools

Purpose of Microbiological Culture

The purpose of microbiological culture is to:
- Isolate and identify microorganisms
- Determine the cause of infectious disease
- Develop vaccines and diagnostic tools

Importance of Artificial Environment in Cell Culture

A favorable artificial environment is important in cell culture because it:
- Supports the growth and survival of cells
- Allows for the control of experimental conditions
- Enables the study of cellular processes and responses

Primary Diagnostic Method of Microbiology

The primary diagnostic method of microbiology used to determine the cause of infectious disease is microbial culture.

Animal Cell Culture

Animal cell culture typically involves the use of:
- Immortalized cells or primary cells
- Cells derived from animal tissues, such as skin or muscle

Causative Agent of Strep Throat

The causative agent of strep throat that can be screened for using a throat culture is Group A beta-hemolytic streptococcus (GABHS).

Applications of Animal Cell Culture

Animal cell culture has found most applications in:
- Biotechnology and biopharmaceutical industries
- Cancer research and therapy
- Tissue engineering and regenerative medicine

Selective Growth of Microorganisms

The term used informally to refer to 'selectively growing' a specific kind of microorganism in the lab is "selective culture".

Animal Cell Culture Technique

The type of biotechnological technique where animal cells are artificially grown in a favorable environment is cell culture.

Enabling Technology in Animal Cell Culture

The development of serum-free media has enabled the working of a wide variety of cells under different conditions in animal cell culture.

Initiation of Organ Culture

Organ culture can be initiated in vitro for animal cell culture using:
- Tissue fragments or explants
- Dissociated cells

Difficulty in Animal Cell Culture

Growing microorganisms on artificial media is generally easier than growing animal cells, because animal cells require more complex and defined media.

Culture of Undifferentiated and Differentiated Cells

Advances in cell culture media and technology have made it possible to culture both undifferentiated and differentiated cells on artificial media in animal cell culture.

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Description

Test your knowledge about cell and microbial culture with this quiz. Learn about the growth of cells in culture and the factors that affect their growth, as well as the method of multiplying microbial organisms in predetermined culture medium under controlled laboratory conditions.