Podcast
Questions and Answers
In Benedict's test, which color change most accurately indicates the presence of reducing sugars?
In Benedict's test, which color change most accurately indicates the presence of reducing sugars?
Barfoed’s test is primarily used to differentiate between which types of carbohydrates?
Barfoed’s test is primarily used to differentiate between which types of carbohydrates?
A positive Seliwanoff’s test, indicated by a red color, denotes the presence of which type of carbohydrate?
A positive Seliwanoff’s test, indicated by a red color, denotes the presence of which type of carbohydrate?
What is the primary function of Molisch's test in carbohydrate analysis?
What is the primary function of Molisch's test in carbohydrate analysis?
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Which characteristic accurately describes carbohydrates?
Which characteristic accurately describes carbohydrates?
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Which of the following accurately describes a characteristic of polysaccharides?
Which of the following accurately describes a characteristic of polysaccharides?
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What is the main role of D-glucose in the human body?
What is the main role of D-glucose in the human body?
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Which statement correctly differentiates between reducing and non-reducing sugars?
Which statement correctly differentiates between reducing and non-reducing sugars?
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What defines the classification of carbohydrates based on complexity?
What defines the classification of carbohydrates based on complexity?
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Which laboratory technique is used for carbohydrate analysis?
Which laboratory technique is used for carbohydrate analysis?
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What is the general chemical formula for most carbohydrates?
What is the general chemical formula for most carbohydrates?
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Which of the following describes monosaccharides?
Which of the following describes monosaccharides?
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The process of breaking down complex carbohydrates into simpler sugars is known as:
The process of breaking down complex carbohydrates into simpler sugars is known as:
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Which of the following statements is true regarding reducing sugars?
Which of the following statements is true regarding reducing sugars?
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What characterizes polysaccharides in terms of their solubility in water?
What characterizes polysaccharides in terms of their solubility in water?
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Which test is specifically used to distinguish between monosaccharides and disaccharides?
Which test is specifically used to distinguish between monosaccharides and disaccharides?
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What color complex does starch form in the iodine test?
What color complex does starch form in the iodine test?
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Which of the following carbohydrates cannot be identified using Molisch's Test?
Which of the following carbohydrates cannot be identified using Molisch's Test?
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Why are large polymers of glucose, such as starch, considered non-reducing sugars?
Why are large polymers of glucose, such as starch, considered non-reducing sugars?
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What type of sugar does Bial’s Test specifically detect?
What type of sugar does Bial’s Test specifically detect?
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Which reducing sugar type reacts the fastest?
Which reducing sugar type reacts the fastest?
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Which statement about Benedict's Test is correct?
Which statement about Benedict's Test is correct?
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Which of the following best describes the reactivity of free anomeric carbons?
Which of the following best describes the reactivity of free anomeric carbons?
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What is produced when pentoses react with Bial's reagent?
What is produced when pentoses react with Bial's reagent?
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Seliwanoff's test is primarily used to distinguish between which types of sugars?
Seliwanoff's test is primarily used to distinguish between which types of sugars?
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What color indicates the presence of ketoses in Seliwanoff's test?
What color indicates the presence of ketoses in Seliwanoff's test?
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Which reagent is a component of Bial's test for detecting pentoses?
Which reagent is a component of Bial's test for detecting pentoses?
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In glucose test strips, elevated glucose levels in urine typically suggest what condition?
In glucose test strips, elevated glucose levels in urine typically suggest what condition?
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What is the main purpose of Benedict's test?
What is the main purpose of Benedict's test?
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What is indicated by a yellow to faint pink color in Seliwanoff's test?
What is indicated by a yellow to faint pink color in Seliwanoff's test?
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What does Molisch's test specifically detect?
What does Molisch's test specifically detect?
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Which of the following best describes the reaction times of reducing monosaccharides compared to disaccharides?
Which of the following best describes the reaction times of reducing monosaccharides compared to disaccharides?
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What is the primary purpose of Seliwanoff's Test?
What is the primary purpose of Seliwanoff's Test?
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Which reagents are used in the Molisch test to detect carbohydrates?
Which reagents are used in the Molisch test to detect carbohydrates?
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What visual indicator is observed in the Molisch test to confirm the presence of carbohydrates?
What visual indicator is observed in the Molisch test to confirm the presence of carbohydrates?
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Which type of sugar does Benedict's test specifically identify?
Which type of sugar does Benedict's test specifically identify?
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What is the result of the reaction in Benedict's test when a reducing sugar is present?
What is the result of the reaction in Benedict's test when a reducing sugar is present?
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In Barfoed’s test, which sugars are identified based on their reaction speed?
In Barfoed’s test, which sugars are identified based on their reaction speed?
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What does the cupric ion do in Barfoed’s test?
What does the cupric ion do in Barfoed’s test?
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Which carbohydrate is specifically identified as a non-reducing sugar in these tests?
Which carbohydrate is specifically identified as a non-reducing sugar in these tests?
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What occurs when molisch’s reagent is added to the sample in the Molisch test?
What occurs when molisch’s reagent is added to the sample in the Molisch test?
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Why must sulfuric acid be added slowly in the Molisch test?
Why must sulfuric acid be added slowly in the Molisch test?
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Flashcards
What are carbohydrates?
What are carbohydrates?
Polyhydroxy aldehydes or ketones, commonly with the formula (CH2O)n, used as a source of energy for the body.
Monosaccharide
Monosaccharide
Simple sugars containing one monosaccharide unit. Examples are glucose and fructose.
Disaccharide
Disaccharide
Complex sugars containing two monosaccharide units. Examples are sucrose and lactose.
Oligosaccharide
Oligosaccharide
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Polysaccharide
Polysaccharide
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Hydrolysis
Hydrolysis
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Reducing sugar
Reducing sugar
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Non-reducing sugar
Non-reducing sugar
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What is Molisch's test?
What is Molisch's test?
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What is Seliwanoff's test?
What is Seliwanoff's test?
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What is Benedict's test?
What is Benedict's test?
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What are reducing sugars?
What are reducing sugars?
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What are non-reducing sugars?
What are non-reducing sugars?
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What is Barfoed's test?
What is Barfoed's test?
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What is a monosaccharide?
What is a monosaccharide?
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What is a disaccharide?
What is a disaccharide?
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What is hydrolysis?
What is hydrolysis?
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What is a mutarotation?
What is a mutarotation?
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Do all disaccharides act as reducing sugars?
Do all disaccharides act as reducing sugars?
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Why are large polymers of glucose like starch not reducing sugars?
Why are large polymers of glucose like starch not reducing sugars?
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How do monosaccharides and disaccharides dissolve in water?
How do monosaccharides and disaccharides dissolve in water?
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Why are polysaccharides less soluble in water than monosaccharides and disaccharides?
Why are polysaccharides less soluble in water than monosaccharides and disaccharides?
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Explain the principle of the iodine test for polysaccharides.
Explain the principle of the iodine test for polysaccharides.
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What are the color changes observed in the iodine test with starch, dextrin, and glycogen?
What are the color changes observed in the iodine test with starch, dextrin, and glycogen?
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What is the purpose of the Molisch's test?
What is the purpose of the Molisch's test?
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What is the purpose of the Benedict's test?
What is the purpose of the Benedict's test?
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How does Barfoed's test distinguish between monosaccharides and disaccharides?
How does Barfoed's test distinguish between monosaccharides and disaccharides?
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What color change in Benedict's test indicates reducing sugars?
What color change in Benedict's test indicates reducing sugars?
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What types of carbohydrates does Barfoed's test differentiate between?
What types of carbohydrates does Barfoed's test differentiate between?
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What does a red color in Seliwanoff's test indicate?
What does a red color in Seliwanoff's test indicate?
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What is Molisch's test used for?
What is Molisch's test used for?
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What is a reducing sugar?
What is a reducing sugar?
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What is Bial's test used for?
What is Bial's test used for?
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What is Seliwanoff's test used for?
What is Seliwanoff's test used for?
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What are glucose test strips used for?
What are glucose test strips used for?
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What is Benedict's test used for?
What is Benedict's test used for?
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How do reducing monosaccharides and disaccharides differ in reaction rates?
How do reducing monosaccharides and disaccharides differ in reaction rates?
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What is Barfoed's test used for?
What is Barfoed's test used for?
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Study Notes
Course Information
- Faculty of Medicine
- Academic Year: 2024-2025
- Year: 1
- Module: Human Body Function (HBF) 102
- Module Code: 102
Carbohydrate Chemistry Lab
- Lab: Carbohydrates chemistry (Lab 2)
- Lecturer: Dr. Hanaa B. Atya
- Department: Biochemistry & Molecular Biology
- Objectives:
- Understand the structure, function, and classification of carbohydrates (monosaccharides, disaccharides, polysaccharides).
- Gain hands-on experience in carbohydrate analysis techniques (e.g., Benedict's test, Barfoed's test).
- Develop the ability to analyze and interpret experimental data, drawing conclusions about the presence and concentration of carbohydrates in lab results.
Carbohydrate Introduction
- Carbohydrates are defined as polyhydroxy aldehydes or polyhydroxy ketones.
- Most carbohydrates in the human body have the formula (CH₂O)ₙ.
- Simple sugars end with an aldehyde or ketone group.
Carbohydrate Classification
- Complexity:
- Simple carbohydrates (one unit): Monosaccharides
- Complex carbohydrates (more than one unit):
- Disaccharides
- Oligosaccharides
- Polysaccharides
- Size:
- Tetroses (4 carbons)
- Pentoses (5 carbons)
- Hexoses (6 carbons)
- Heptoses (7 carbons)
- C=O Function:
- Aldoses (aldehyde group)
- Ketoses (ketone group)
- Reactivity:
- Reducing sugars (oxidized by Tollens' reagent, Benedict's reagent or Fehling's reagents)
- Non-reducing sugars (not oxidized by those reagents)
A - Classification based on Complexity
- Simple sugars (one unit): Monosaccharides.
- Complex sugars (more than one):
- Disaccharides (two monosaccharide units)
- Oligosaccharides (3-9 monosaccharide units)
- Polysaccharides (more than 9 monosaccharide units)
- Complex carbohydrates can be broken down into smaller units through hydrolysis.
B - Classification based on Reactivity
- Reducing sugars: The anomeric carbon's oxygen is not attached to any other structure. They can act as reducing agents.
- Non-reducing sugars: The anomeric carbon's oxygen is attached to another structure. They cannot act as reducing agents.
Solubility of Sugars
- Monosaccharides and disaccharides are freely dissolved in water due to its polarity
- Polysaccharides are not freely dissolved in water because their high molecular weight forms colloidal solutions.
Iodine Test
- The test distinguishes mono- and disaccharides from certain polysaccharides (starch, dextrin, glycogen).
- Polyiodide ions form colored adsorption complexes with glucose residues of polysaccharides.
- Monosaccharides, disaccharides, and unbranched polysaccharides are colorless in presence of iodine.
Chemical Properties
- Molisch's Test: To identify carbohydrates from other macromolecules (lipids, proteins).
- Benedict's Test: To detect reducing sugars.
- Barfoed's Test: To distinguish between monosaccharides and disaccharides (based on reaction time), detecting reducing sugars.
- Bial's Test: To distinguish between pentoses and hexoses (detecting pentoses).
- Seliwanoff's Test: To differentiate between aldoses and ketoses (detecting ketoses).
Experiment 1: Molisch's Test
- Objective: Identify carbohydrates from other macromolecules (lipids, proteins).
- Principle: Acid dehydrates the sugar and reacts with alpha-naphthol to produce a purple product.
Experiment 2: Benedict's Test
- Objective: Distinguish between reducing and non-reducing sugars.
- Principle: Cu²⁺ in alkaline medium oxidizes reducing sugars (leading to color change).
Experiment 3: Barfoed's Test
- Objective: Distinguish between monosaccharides and disaccharides (in terms of reaction time)
- Principle: Cu²⁺ in slightly acidic medium reacts with reducing sugars and forms cuprous oxide
Experiment 4: Bial's Test
- Objective: Differentiate between pentoses and hexoses.
- Principle: Bial's reagent dehydrates pentoses to form furfural, which reacts further to produce a bluish or green product.
Experiment 5: Seliwanoff's Test
- Objective: Distinguish between aldoses and ketoses.
- Principle: Ketohexoses react faster with Seliwanoffs reagent to form cherry red products while aldoses to form yellow/pink products.
Glucose Test Strips
- Measure glucose (sugar) in a person's urine.
- Monitor glucose levels in people with diabetes.
Practical Work
- Two biological samples will be provided (A & B).
- Test for the presence of glucose using the tests.
Interactive Questions
- Questions related to monosaccharides, disaccharides, carbohydrates, and the different tests.
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Description
Test your knowledge on carbohydrate analysis and identification through various laboratory tests like Benedict's, Barfoed’s, and Seliwanoff’s. This quiz covers the characteristics of carbohydrates, their classification, and their functions in human body. Perfect for students studying biochemistry or related fields.