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Questions and Answers
What is the purpose of using restriction enzymes in genome mapping?
What is the purpose of using restriction enzymes in genome mapping?
- To obtain small fragments
- To obtain large fragments (correct)
- To obtain fragments with overlapping sequences
- To obtain fragments from a specific chromosome
What is a genomic library?
What is a genomic library?
- A collection of large DNA fragments
- A collection of all DNA fragments from a single chromosome
- A collection of all DNA fragments from the entire genome (correct)
- A collection of small DNA fragments
What is the purpose of using a radioactive probe in genome mapping?
What is the purpose of using a radioactive probe in genome mapping?
- To identify other fragments with overlapping sequences (correct)
- To create a genomic library
- To identify the fragment of interest
- To cut the fragment of interest
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Study Notes
- Large maps of genomes require manageable mapped segments
- Chromosome walks are taken on large DNA fragments
- Restriction enzymes are used to obtain large fragments
- The fragments are cloned into hybrid plasmids to create a genomic library
- The library contains pieces of DNA from the entire genome
- The fragment of interest is cut with other restriction enzymes and sequenced
- A radioactive probe is used to identify other fragments with overlapping sequences
- These fragments are similarly mapped
- The process allows for the mapping of large segments or entire genomes
- The task can be overwhelming but is essential for genome mapping.
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