Questions and Answers
What is the magnification of the ocular lens?
What is the magnification of the scanning lens?
What is the magnification of the low power lens?
What is the magnification of the high power lens?
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What is the magnification of the oil immersion lens?
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What is total magnification when using a 10x ocular lens and a 45x objective lens?
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What is the total magnification of the scanning lens?
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What is the total magnification of the low power lens?
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What is the total magnification of the high power lens?
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What is the total magnification of the oil immersion lens?
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What does depth of field refer to?
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What does parfocal mean?
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What is working distance in microscopy?
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What is the eyepiece lens?
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Study Notes
Magnification Overview
- Ocular lens magnification is 10x, crucial for viewing specimens.
- Scanning lens has a magnification of 4x, useful for initial observations.
- Low Power lens magnifies at 10x, allowing for a wider view of specimens.
- High Power lens provides a magnification of 40x, suitable for detailed study.
- Oil Immersion lens achieves a magnification of 100x, enhancing resolution for fine details.
Total Magnification
- Total Magnification (TM) is calculated by multiplying the ocular lens power with the objective lens power.
- For instance, using a 10x ocular lens and a 45x objective lens results in a total magnification of 450x (10 x 45).
Total Magnifications for Different Lenses
- TM of Scanning lens equals 40x (10 x 4).
- TM of Low Power lens equals 100x (10 x 10).
- TM of High Power lens equals 400x (10 x 40).
- TM of Oil Immersion lens equals 1000x (10 x 100).
Microscopy Terms
- Depth of Field refers to the thickness of a specimen that remains in focus, critical for viewing multi-layered samples.
- Parfocal lenses keep specimens nearly in focus when switching between magnification powers, enhancing usability.
- Working distance is the distance between the slide surface and the objective lens, important for avoiding damage to the slide.
- Field refers to the area viewed through the eyepiece lens, typically magnified at 10x.
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Description
This set of flashcards focuses on the magnification values of different microscopes' lenses. Learn the specifications of ocular, scanning, low power, high power, and oil immersion lenses. Perfect for biology students to master microscope terminology.
