BTE 204 Fundamentals of Genetic Engineering: Introduction Quiz
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Questions and Answers

What is the main function of DNA ligase?

  • Distinguish between transformed cells
  • Quantify gene expression
  • Join separate DNA molecules (correct)
  • Cleave DNA into fragments

What is the role of restriction enzymes in bacteria and archaea?

  • Join DNA molecules
  • Distinguish between transformed cells
  • Cleave DNA into fragments (correct)
  • Quantify gene expression

Which type of cloning vector is mainly used in bacterial cloning?

  • HAC
  • YAC
  • BAC (correct)
  • Plasmid

What is the purpose of a selective marker in genetic engineering?

<p>Distinguish between transformed and non-transformed cells (C)</p> Signup and view all the answers

Which enzyme recognizes specific short nucleotide sequences known as restriction sites?

<p>Restriction enzyme (D)</p> Signup and view all the answers

What is the function of a reporter gene in genetic engineering?

<p>Quantify gene expression (C)</p> Signup and view all the answers

What is the main purpose of DNA extraction?

<p>To quantify the concentration and/or mass of DNA extracted (C)</p> Signup and view all the answers

Which scientist is credited with inventing the polymerase chain reaction (PCR)?

<p>Kary Mullis (D)</p> Signup and view all the answers

What is the term used to describe the process of multiplying the number of copies of a DNA molecule?

<p>DNA Amplification (A)</p> Signup and view all the answers

During which step of PCR does the specific DNA sequence get cloned to generate multiple copies?

<p>Elongation (C)</p> Signup and view all the answers

What is the role of probes in DNA hybridization?

<p>To bind to specific target sequences (C)</p> Signup and view all the answers

What is the primary aim of hybridization in molecular biology?

<p>To bind complementary nucleotide sequences together (B)</p> Signup and view all the answers

What is the main purpose of Genetic Engineering?

<p>Making deliberate changes in an organism's genetic material (D)</p> Signup and view all the answers

Why are organisms generated through Genetic Engineering considered genetically modified (GM)?

<p>As they are created by deliberate changes in their genetic material (D)</p> Signup and view all the answers

Which term is used to refer to an organism created through Genetic Engineering?

<p>Genetic modified organism (GMO) (A)</p> Signup and view all the answers

What are some key components required for recombinant DNA technology?

<p>Identifying and cutting specific DNA segments (D)</p> Signup and view all the answers

What is the significance of Werner Arber's discovery of restriction enzymes in Genetic Engineering?

<p>To identify specific segments of DNA (C)</p> Signup and view all the answers

What was the breakthrough achievement resulting from recombinant DNA technology?

<p>Production of human insulin (B)</p> Signup and view all the answers

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