BTE 204 Fundamentals of Genetic Engineering: Introduction Quiz
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Questions and Answers

What is the main function of DNA ligase?

  • Distinguish between transformed cells
  • Quantify gene expression
  • Join separate DNA molecules (correct)
  • Cleave DNA into fragments

    • What is the role of restriction enzymes in bacteria and archaea?

  • Join DNA molecules
  • Distinguish between transformed cells
  • Cleave DNA into fragments (correct)
  • Quantify gene expression

    • Which type of cloning vector is mainly used in bacterial cloning?

  • HAC
  • YAC
  • BAC (correct)
  • Plasmid

    • What is the purpose of a selective marker in genetic engineering?

    <p>Distinguish between transformed and non-transformed cells</p> Signup and view all the answers

    Which enzyme recognizes specific short nucleotide sequences known as restriction sites?

    <p>Restriction enzyme</p> Signup and view all the answers

    What is the function of a reporter gene in genetic engineering?

    <p>Quantify gene expression</p> Signup and view all the answers

    What is the main purpose of DNA extraction?

    <p>To quantify the concentration and/or mass of DNA extracted</p> Signup and view all the answers

    Which scientist is credited with inventing the polymerase chain reaction (PCR)?

    <p>Kary Mullis</p> Signup and view all the answers

    What is the term used to describe the process of multiplying the number of copies of a DNA molecule?

    <p>DNA Amplification</p> Signup and view all the answers

    During which step of PCR does the specific DNA sequence get cloned to generate multiple copies?

    <p>Elongation</p> Signup and view all the answers

    What is the role of probes in DNA hybridization?

    <p>To bind to specific target sequences</p> Signup and view all the answers

    What is the primary aim of hybridization in molecular biology?

    <p>To bind complementary nucleotide sequences together</p> Signup and view all the answers

    What is the main purpose of Genetic Engineering?

    <p>Making deliberate changes in an organism's genetic material</p> Signup and view all the answers

    Why are organisms generated through Genetic Engineering considered genetically modified (GM)?

    <p>As they are created by deliberate changes in their genetic material</p> Signup and view all the answers

    Which term is used to refer to an organism created through Genetic Engineering?

    <p>Genetic modified organism (GMO)</p> Signup and view all the answers

    What are some key components required for recombinant DNA technology?

    <p>Identifying and cutting specific DNA segments</p> Signup and view all the answers

    What is the significance of Werner Arber's discovery of restriction enzymes in Genetic Engineering?

    <p>To identify specific segments of DNA</p> Signup and view all the answers

    What was the breakthrough achievement resulting from recombinant DNA technology?

    <p>Production of human insulin</p> Signup and view all the answers

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