BTB Indicator and Gas Exchange
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Questions and Answers

Bromothymol blue (BTB) is used to indicate the presence of certain gases in water. What does yellow BTB indicate?

  • High levels of carbon dioxide (CO2). (correct)
  • High levels of oxygen (O2).
  • Equal levels of oxygen (O2) and carbon dioxide (CO2).
  • The absence of both oxygen (O2) and carbon dioxide (CO2).

A test tube containing a plant stays blue after 24 hours. What does this indicate about the gas concentration in the test tube?

  • A balanced level of oxygen (O2) and carbon dioxide (CO2).
  • Dominant presence of carbon dioxide (CO2).
  • Dominant presence of oxygen (O2). (correct)
  • Absence of gas exchange.

In an experiment with plants in a dark environment, what change in gas levels would you expect to observe, and what color change would you see in the BTB indicator?

  • Decrease in O2, water turns pale green. (correct)
  • Increase in CO2, water turns blue.
  • Increase in O2, water turns blue.
  • Decrease in CO2, water turns yellow.

Snails are placed in a test tube under light conditions; what effect would this have on the oxygen levels and the color of the water?

<p>O2 levels decrease; water turns yellow. (A)</p> Signup and view all the answers

Why did the plants in the experiment eventually turn brown when kept under specific experimental conditions?

<p>Lack of proper conditions. (B)</p> Signup and view all the answers

A setup with both plants and snails is kept off lights. What is the likely outcome for oxygen levels and the organisms?

<p>O2 drops to zero, both organisms die. (D)</p> Signup and view all the answers

In the carbon dioxide-oxygen cycle, what roles do plants and animals play concerning these gases?

<p>Animals take in O2 and release CO2; plants take in CO2 and release O2 in sunlight. (A)</p> Signup and view all the answers

Which statement accurately describes the gas release pattern of plants and animals in different lighting conditions?

<p>Plants release O2 in light and CO2 in dark; animals release CO2 continuously. (C)</p> Signup and view all the answers

During the Calvin Cycle, what is the immediate result of CO₂ combining with RuBP?

<p>Creation of an unstable 6-carbon compound that splits into two 3-PGA molecules. (B)</p> Signup and view all the answers

How many turns of the Calvin Cycle are required to produce one molecule of glucose?

<p>6 turns (C)</p> Signup and view all the answers

In photosynthesis, which of the following statements accurately describes the roles of H₂O and CO₂?

<p>H₂O is oxidized to O₂, while CO₂ is reduced to form glucose. (C)</p> Signup and view all the answers

During the reduction phase of the Calvin Cycle, what is the role of NADPH?

<p>It donates electrons to convert 1,3-BPG into glyceraldehyde-3-phosphate (G3P). (A)</p> Signup and view all the answers

After 6 molecules of CO₂ have entered the Calvin cycle, how many molecules of G3P are produced, and how many exit the cycle to be used for glucose synthesis?

<p>12 G3P produced, 2 G3P exit. (A)</p> Signup and view all the answers

What is the primary function of the regeneration phase in the Calvin Cycle?

<p>To regenerate RuBP from G3P, ensuring the cycle can continue. (B)</p> Signup and view all the answers

In an experiment using bromothymol blue (BTB), a scientist observes that a solution containing BTB turns yellow. What does this color change indicate about the conditions of the solution?

<p>The solution is acidic due to high CO₂ levels. (A)</p> Signup and view all the answers

How did the oxygen-18 isotope tracing experiment contribute to our understanding of photosynthesis?

<p>Showed that O₂ produced during photosynthesis comes from H₂O. (B)</p> Signup and view all the answers

During the light-dependent reactions of photosynthesis, what is the primary role of water photolysis?

<p>To replenish electrons lost by chlorophyll a in Photosystem II. (D)</p> Signup and view all the answers

How does the electron transport chain (ETC) contribute to ATP synthesis during the light-dependent reactions?

<p>By using the energy from electron transfer to pump H⁺ ions into the thylakoid lumen, creating a proton gradient. (C)</p> Signup and view all the answers

In the Calvin Cycle, what is the function of RuBisCO (Ribulose-1,5-bisphosphate carboxylase/oxygenase)?

<p>To catalyze the initial fixation of carbon dioxide by attaching it to RuBP. (D)</p> Signup and view all the answers

If a plant were exposed to only green light, what would be the immediate consequence regarding photosynthesis?

<p>The rate of photosynthesis would significantly decrease as chlorophyll absorbs red and blue light more effectively. (A)</p> Signup and view all the answers

What is the ultimate source of electrons that are passed along the electron transport chain during the light-dependent reactions of photosynthesis?

<p>Water molecules through photolysis. (D)</p> Signup and view all the answers

How would a decrease in the concentration of NADP⁺ in the stroma directly affect the light-dependent reactions?

<p>It would slow down the electron flow from Photosystem I, limiting NADPH production. (C)</p> Signup and view all the answers

Which of the following accurately describes the relationship between the light-dependent reactions and the Calvin cycle?

<p>The light-dependent reactions provide ATP and NADPH, which are used in the Calvin cycle to convert CO₂ into glucose. (B)</p> Signup and view all the answers

In conditions where the stroma becomes more acidic (lower pH) than usual, what is the most likely immediate effect on the Calvin cycle?

<p>Impairment of enzyme activity, including RuBisCO, reducing the efficiency of carbon fixation. (B)</p> Signup and view all the answers

In an Elodea and snail ecosystem within a closed test tube, what is the primary reason for the observed stability in both $O_2$ and $CO_2$ levels, close to 6 ppm, when the system is exposed to light?

<p>The rate of photosynthesis in Elodea is precisely balanced by the rate of aerobic respiration of the snail. (C)</p> Signup and view all the answers

If an Elodea plant is placed in a sealed container filled with water, what will happen to the levels of $O_2$ and $CO_2$ in the water if the container is placed in complete darkness for an extended period?

<p>$O_2$ levels will decrease and $CO_2$ levels will increase, due to the plant's respiration. (B)</p> Signup and view all the answers

In a system containing Elodea plants and snails, what would be the immediate effect on the concentration of carbon dioxide ($CO_2$) if the plants were suddenly removed?

<p>An increase in $CO_2$ concentration as the snails continue to respire but the plants no longer photosynthesize. (A)</p> Signup and view all the answers

Consider a closed aquatic ecosystem with Elodea plants and snails. If the total amount of oxygen and carbon dioxide in the system is 12 ppm, and the oxygen level is observed to increase, what corresponding change would be expected in the carbon dioxide level, assuming the total gas amount remains constant?

<p>The carbon dioxide level would decrease, compensating for the rise in oxygen to maintain the 12 ppm total. (A)</p> Signup and view all the answers

Which of the following equations best represents cellular respiration?

<p>$C_6H_{12}O_6 + 6O_2 \rightarrow 6CO_2 + 6H_2O + energy$ (A)</p> Signup and view all the answers

In a balanced ecosystem of Elodea and snails, what effect would increasing the number of snails have on the Elodea if the light intensity remains constant?

<p>The Elodea would grow faster due to the increased carbon dioxide production by the snails. (C)</p> Signup and view all the answers

How does photosynthesis contribute to maintaining a stable carbon-oxygen balance in an environment with both plants and animals?

<p>By consuming carbon dioxide and producing oxygen. (C)</p> Signup and view all the answers

In an experiment with Elodea plants and snails in a sealed tube, a student observes that both oxygen and carbon dioxide levels have significantly decreased. What is the most likely explanation for this observation?

<p>A leak in the system allowed gases to escape. (A)</p> Signup and view all the answers

Which of the following correctly describes the flow of electrons during the light-dependent reactions of photosynthesis?

<p>PSII → electron transport chain → PSI → NADP⁺ reductase. (C)</p> Signup and view all the answers

What is the direct role of the proton gradient created within the thylakoid lumen during the light-dependent reactions?

<p>To provide the energy for ATP synthase to produce ATP. (C)</p> Signup and view all the answers

In the Calvin cycle, what is the primary function of RuBisCO?

<p>To fix carbon dioxide by attaching it to RuBP. (A)</p> Signup and view all the answers

How do the light-dependent reactions directly support the Calvin cycle?

<p>By supplying ATP and NADPH required for the cycle. (A)</p> Signup and view all the answers

During photosynthesis, at which point is light energy converted into chemical energy?

<p>When electrons are excited in chlorophyll by photons. (C)</p> Signup and view all the answers

What would be the immediate impact on the Calvin cycle if a plant cell suddenly ran out of ATP?

<p>The regeneration of RuBP would be inhibited. (C)</p> Signup and view all the answers

If a plant is exposed to a toxin that inhibits the function of NADP⁺ reductase, what would be the most direct consequence?

<p>Decreased production of NADPH. (C)</p> Signup and view all the answers

During the Calvin cycle, what is the immediate result of the combination of $CO_2$ with RuBP?

<p>The formation of 3-phosphoglycerate (3-PGA). (A)</p> Signup and view all the answers

Which of the following statements accurately describes the role of ATP and NADPH in the Calvin cycle?

<p>ATP phosphorylates 3-PGA, and NADPH donates electrons to reduce 1,3-BPG into G3P. (D)</p> Signup and view all the answers

How many turns of the Calvin cycle are required to produce one molecule of glucose ($C_6H_{12}O_6$)?

<p>6 turns (B)</p> Signup and view all the answers

In photosynthesis, which molecule is oxidized and which is reduced?

<p>$H_2O$ is oxidized and $CO_2$ is reduced. (D)</p> Signup and view all the answers

What was the key finding of the Oxygen-18 isotope tracing experiment regarding photosynthesis?

<p>Water is the source of oxygen produced during photosynthesis. (A)</p> Signup and view all the answers

How are sugars transported to nonphotosynthetic cells, and in what form are excess sugars stored?

<p>As sucrose, stored as starch. (B)</p> Signup and view all the answers

Which process describes cells using oxygen to break down glucose for energy production?

<p>Aerobic respiration (C)</p> Signup and view all the answers

What color change would be observed in bromothymol blue (BTB) indicator when $CO_2$ concentration increases in a solution?

<p>The BTB would turn yellow. (D)</p> Signup and view all the answers

In an experiment using Bromothymol blue (BTB) as an indicator, what color change would you expect to observe in a test tube containing a snail after a period of increased respiration?

<p>The solution turns yellow, indicating increased carbon dioxide levels. (B)</p> Signup and view all the answers

A sealed test tube contains a plant and Bromothymol blue solution. If the test tube is placed in direct sunlight, what change in gas concentration and solution color do you anticipate?

<p>Oxygen levels increase, and the solution turns blue. (A)</p> Signup and view all the answers

Under which of the following conditions would a plant primarily release carbon dioxide ($CO_2$)?

<p>During periods of darkness when photosynthesis cannot occur. (B)</p> Signup and view all the answers

A researcher observes that a plant in a sealed container filled with Bromothymol blue solution eventually turns brown and dies, despite initial exposure to light. What is the most likely reason for this?

<p>Depletion of other essential resources or buildup of toxic substances. (C)</p> Signup and view all the answers

Consider a closed ecosystem containing only plants and snails in water. If the system is placed in complete darkness, what is the likely long-term outcome?

<p>Both the plant and snail populations will decline due to lack of energy production. (B)</p> Signup and view all the answers

How do plants and animals depend on each other through the exchange of gases?

<p>Plants use carbon dioxide released by animals, and animals use oxygen released by plants. (C)</p> Signup and view all the answers

In terms of the oxygen-carbon dioxide cycle, which of the following statements correctly describes the role of animals?

<p>Animals consume oxygen and release carbon dioxide during respiration. (D)</p> Signup and view all the answers

Consider a scenario where a large forest is removed and replaced with a parking lot. How would this change most significantly impact the local oxygen-carbon dioxide cycle?

<p>Reduced oxygen production and increased carbon dioxide levels due to loss of photosynthetic activity. (D)</p> Signup and view all the answers

In an environment containing only Elodea and snails, what is the primary reason for the stabilization of both oxygen and carbon dioxide levels when exposed to light?

<p>The Elodea's photosynthetic rate balances the consumption of oxygen by the snail's respiration. (D)</p> Signup and view all the answers

If Elodea and snails are placed in a closed test tube in the dark, which of the following changes in gas levels would be expected and why?

<p>Oxygen levels decrease, carbon dioxide levels increase, due to respiration surpassing photosynthesis. (C)</p> Signup and view all the answers

Considering the interdependence of plants and animals, what direct effect does an increase in the plant population have on the animal population in a closed environment?

<p>The animal population increases due to higher oxygen production by plants. (C)</p> Signup and view all the answers

In a balanced ecosystem containing plants and animals, what would be the immediate effect of drastically reducing the plant population on the carbon dioxide levels?

<p>Carbon dioxide levels would increase because fewer plants would be available to absorb it. (B)</p> Signup and view all the answers

Given the equation for photosynthesis, $6CO_2 + 6H_2O + light \rightarrow C_6H_{12}O_6 + 6O_2$, what happens to the rate of this reaction if the amount of available light is significantly reduced?

<p>The rate of photosynthesis decreases directly with the amount of light available. (A)</p> Signup and view all the answers

If the total amount of oxygen and carbon dioxide in a closed system containing Elodea is 12 ppm, and the oxygen level is measured at 8 ppm, what is the most likely level of carbon dioxide in the system?

<p>4 ppm (A)</p> Signup and view all the answers

Aerobic respiration is represented by the equation $C_6H_{12}O_6 + 6O_2 \rightarrow 6CO_2 + 6H_2O + energy$. If an animal's access to oxygen is limited, how is the process of aerobic respiration affected?

<p>Aerobic respiration is inhibited, leading to a potential buildup of glucose and a decrease in energy production. (D)</p> Signup and view all the answers

How does the interdependence of photosynthesis and aerobic respiration contribute to the stability of an ecosystem?

<p>Photosynthesis and aerobic respiration recycle oxygen and carbon dioxide, maintaining gas balance. (C)</p> Signup and view all the answers

Flashcards

Photosynthesis

Process where plants convert light energy into chemical energy (glucose) using CO₂ and water, releasing oxygen.

Light-Dependent Reactions

Captures solar energy and converts it into ATP and NADPH, releasing oxygen.

Calvin Cycle

Uses ATP and NADPH to fix CO₂ into organic molecules like glucose.

Thylakoid Membrane

Site of light-dependent reactions; contains chlorophyll.

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Photosystem II (PSII)

Absorbs light energy, exciting electrons in chlorophyll a; splits water.

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Photosystem I (PSI)

Uses light energy to re-excite electrons; reduces NADP+ to NADPH.

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ATP Synthase

Uses the proton gradient to phosphorylate ADP, forming ATP.

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RuBisCO

Fixes carbon dioxide by attaching it to RuBP.

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Interdependence

Mutual reliance between organisms or systems.

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BTB (Bromothymol blue)

Indicates gas presence dissolved in water by color.

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Yellow water (with BTB)

High level of CO2

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Blue water (with BTB)

High level of O2

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Plant gas exchange

Plants take in CO2, release O2 in light; opposite in dark.

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Animal gas exchange

Animals take in O2 and release CO2.

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CO2-O2 cycle

Animals exhale CO2, which plants use. Plants release O2, which animals use

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O2 and CO2 roles

Animals require O2 and release CO2. Plants use CO2 for photosynthesis and release O2.

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Stable Environment

A balanced number of plants and snails keeps O2 and CO2 levels stable.

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Elodea in Light vs. Dark

In light, plants use CO2 and release O2. In dark, they use O2 and release CO2 through respiration.

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Constant Gas Levels

The total quantity of O2 and CO2 remains constant because photosynthesis and respiration balance each other.

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Photosynthesis Equation

6CO2 + 6H2O + light energy → C6H12O6 + 6O2

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Aerobic Respiration

C6H12O6 + 6O2 → 6CO2 + 6H2O + energy

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Complementary Processes

Photosynthesis and respiration are complementary. O2 produced by plants is used by animals, and CO2 released by animals is used by plants

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CO₂ Fixation

CO₂ combines with RuBP to form an unstable 6-carbon compound, which splits into two 3-PGA molecules.

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Calvin Cycle: Reduction

ATP phosphorylates 3-PGA into 1,3-BPG. NADPH donates electrons to convert 1,3-BPG into G3P. One G3P exits, five regenerate RuBP.

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RuBP Regeneration

Five G3P molecules rearrange to form three RuBP molecules, which requires ATP.

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Glucose Formation

Six turns of the Calvin Cycle are required to produce two G3P molecules, which combine to form one molecule of glucose.

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Photosynthesis Redox

Water is oxidized to oxygen, and carbon dioxide is reduced to glucose, driven by light energy.

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Water Splitting

O₂ comes from H₂O, not CO₂.This was confirmed using Oxygen-18 isotope tracing.

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Bromothymol Blue (BTB)

A pH indicator that turns yellow in acidic conditions (high CO₂) and blue in basic conditions (high O₂).

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Light Reactions Purpose

Converts light energy to chemical energy (ATP and NADPH), releasing O₂.

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Light Reactions Location

Thylakoid membranes within the chloroplast.

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PSII Function

Absorbs light, excites electrons, and splits water to replenish those electrons.

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Electron Transport Chain (ETC)

Transports electrons, pumps H⁺ to create a gradient for ATP synthesis.

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ATP Synthase Function

Utilizes H⁺ gradient to phosphorylate ADP into ATP.

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PSI Function

Re-excites electrons and forms NADPH.

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Calvin Cycle Purpose

Uses ATP and NADPH to incorporate CO₂ into organic molecules (glucose).

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Calvin Cycle Location

Stroma of the chloroplast.

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Elodea: Light vs. Dark

In light, plants use CO2 and release O2. In the dark, they use O2 and release CO2 through respiration.

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BTB Indicator

Indicates gas levels in water, turning yellow with high CO2 and blue with high O2, green when levels are equal.

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Plant Gas Release

In light, plants release O2. In dark, plants release CO2.

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Animal Gas Release

Animals release carbon dioxide (CO2).

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Plants in Light

Plants release oxygen (O2) in the presence of light.

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Plants in Dark

Plants release carbon dioxide (CO2) when in dark conditions.

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Glyceraldehyde-3-Phosphate (G3P)

The net product after reduction in the Calvin Cycle, used to create glucose or regenerate RuBP.

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Calvin Cycle Function

Uses ATP and NADPH to convert CO₂ into sugars.

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CO₂-O₂ Cycle

Animals release carbon dioxide, which plants use for photosynthesis; plants release oxygen, which animals use.

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NADPH Role

Converts 1,3-BPG into G3P.

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Photosynthesis Electron Flow

Photosynthesis reverses the electron flow of cellular respiration.

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Water Oxidation

Process where water is split and oxygen is released to the enviornment.

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