Body Fluid Counts and Differentials Quiz

Podcast

Play an AI-generated podcast conversation about this lesson

Questions and Answers

What should be done if deterioration of the Sysmex XN Check BF control is suspected?

Call the Sysmex Technical Assistance Center and inform the department supervisor. (correct)

Discard the control vial immediately without further checks.

Store the control at room temperature to prevent further damage.

Continue using the control until results are received.

Which of the following is NOT a recommended practice for the Sysmex XN Check BF controls?

Storing the controls in frozen conditions. (correct)

Using controls only within their expected assay ranges.

Maintaining the recommended temperature range for storage.

Discarding vials confirmed to be damaged.

What action should be taken if a control vial of Quantimetrix Spinalscopics shows signs of microbial contamination?

Notify the supervisor but continue using the vial.

Use the vial after sterilizing it.

Retest the control before deciding on discarding it.

Discard the control vial immediately. (correct)

What is indicated by the mean of a control result being outside the expected assay ranges?

Deterioration of the control may be suspected. Signup and view all the answers

Which of the following statements about automated and manual fluid control levels is correct?

Levels 1 and 2 are available for both Sysmex XN and Quantimetrix. Signup and view all the answers

What is the effective date of the current version 8.0 of the Body Fluid Counts and Differentials procedure?

6/12/2024 Signup and view all the answers

Which of the following steps is NOT included in routine body fluid analysis?

Bacterial culture Signup and view all the answers

What must be used if similar results are not produced when running specimens in duplicate?

Manual counts Signup and view all the answers

What is the status of version 7.0 of the procedure?

Retired Signup and view all the answers

For which type of analysis are validated fluid sample types analyzed on Sysmex XN Hematology analyzers?

Total WBC and RBC counts Signup and view all the answers

Which device is utilized for manual counts of lower cell counts?

Hemacytometer Signup and view all the answers

What type of staining is used for manual differential counts?

Wright's staining Signup and view all the answers

Which of the following represents the timeline for version 8.0?

Approved now, Effective later Signup and view all the answers

When performing a 100 cell differential, what should you do if it is not possible?

Set count to a lesser number under ‘Set Count’. Signup and view all the answers

What is the primary function of the MEM in reporting results?

To record body fluid counts. Signup and view all the answers

What is required for slides showing abnormal results?

They must be reviewed by a pathologist. Signup and view all the answers

What is indicated if results are abnormal or concerning during reporting?

Delivery and/or call to the pathologist is required for immediate review. Signup and view all the answers

What fluid types are mentioned that might require a differential count?

CSF and synovial fluid. Signup and view all the answers

What should be done with the Path Review form after completion?

It must be submitted for review. Signup and view all the answers

What action is NOT recommended before completing a fluid differential?

Change the fluid type mid-test. Signup and view all the answers

What is the first step in performing a 100 cell differential?

Select the type of fluid on the GUI keyboard. Signup and view all the answers

What is the depth of the cell counting chamber in a hemacytometer?

0.100 mm Signup and view all the answers

What is the volume of the sample chamber in a hemacytometer?

0.1 Microliter Signup and view all the answers

Which ruling pattern is used in the metallized hemacytometer?

Improved Neubauer Signup and view all the answers

How many small squares are in each group within the hemacytometer's central square millimeter?

16 Signup and view all the answers

What is the total area covered by the rulings in a hemacytometer?

9 square millimeters Signup and view all the answers

Which of the following descriptions correctly identifies the groups in the hemacytometer's ruling?

The middle line of the triple lines acts as a boundary. Signup and view all the answers

What is indicated by the ruling lines in a hemacytometer?

The volume for each small square Signup and view all the answers

When does the specified version of the Body Fluid Counts and Differentials procedures become effective?

6/12/2024 Signup and view all the answers

What is the minimum number of white cells required in CSF to prepare a cytosmear slide?

5 white cells Signup and view all the answers

What type of stain is used for staining the slide after smear preparation?

Wright's stain Signup and view all the answers

Which fluid appearance requires using 1-3 drops for slide preparation?

Cloudy fluid Signup and view all the answers

When preparing a cytosmear slide, what is added to the sample chamber to potentially reduce cell distortion?

22% bovine serum albumin Signup and view all the answers

At what relative centrifugal force (RCF) should the cytocentrifuge be set for processing the fluid?

1500 RCF Signup and view all the answers

What should be labeled on the cytosmear slide?

Patient name, accession number, date, and tech code Signup and view all the answers

If the fluid is bloody, how should it be diluted for slide preparation?

1:5 saline dilution Signup and view all the answers

What action must be taken with the sample chamber after it is filled?

Cap the chamber and place in cytopsin Signup and view all the answers

Which of the following fluid types is classified under the LIS code 'PTDFL'?

Peritoneal Dialysis Signup and view all the answers

What is the LIS code for colorless fluids?

COL Signup and view all the answers

Which site is responsible for all fluid types according to the provided content?

RMANH Signup and view all the answers

Which fluid type corresponds to the LIS code 'SYN'?

Synovial Signup and view all the answers

What color does the LIS code 'AMBER' represent?

Amber Signup and view all the answers

Which of the following is NOT a type of body fluid mentioned?

Lymphatic Signup and view all the answers

What fluid type is represented by the LIS code 'JP'?

Jackson Pratt Signup and view all the answers

The LIS code 'GRN' corresponds to which color of fluid?

Green Signup and view all the answers

Which site is primarily used for spinal fluids?

SFWC Signup and view all the answers

What type of fluid is associated with the LIS code 'PFLD'?

Cerebral Spinal Signup and view all the answers

Which LIS code should be used if a code isn't available for a fluid type?

FREE TEXT Signup and view all the answers

What is the LIS code for pleural fluids?

PLEU Signup and view all the answers

Which color of fluid is represented by the LIS code 'PINK'?

Pink Signup and view all the answers

For which type of fluid is the LIS code 'BROWN' used?

Brown Signup and view all the answers

Study Notes

Document Title and Number

Title: Body Fluid Counts and Differentials – Automated and Manual
Number: HEME-0001

Revision and Approval

Revision: 8.0
Approval Date: 6/12/2024
Approved By: Clifton Bridges Laboratory Director SMH RGH (CLBRIDGE)
Reviewed By: Clifton Bridges Laboratory Director SMH RGH (CLBRIDGE)

Review and Completion Dates

Last Approval or Periodic Review Completed: 6/12/2024
Next Periodic Review Needed On or Before: 6/12/2025
Review Date: 6/12/2024
Effective Date: 6/12/2024

Performed By

Performed By: Clifton Bridges Laboratory Director SMH RGH (CLBRIDGE), Mark Williams Laboratory Director (WILL1050), Jack Wilson Laboratory Director SPEC RCH (JJWILSON), Angie Duong Laboratory Director (ADUONG), Jeffery Green MD (JTGREEN), Lindsey Matthews Laboratory Director (MATT0083), Cora G Uram-Tuculescu Laboratory Director (URAM0000), Thuy Sandy Pham Education Coordinator (101706)

Printed By

Printed By: Leetta Bateman Lead MLS (12/12/2024)

Organization

Organization: Richmond Market

Specimen Collection

CSF: Submitted in 3 sterile tubes (labeled sequentially) - Tube 1: Chemical & Immunologic studies, Tube 2: Microbiologic examination, Tube 3: Cell Count and Cytological examination
Other fluids (pleural, peritoneal, pericardial): Collected in EDTA tube or non-additive container (3-7mL volume ideal)
Crystal analysis: Red top tube or non-additive container; avoid EDTA
If EDTA is the only option for analysis, note.
Include body fluids from other sites (e.g., bronchial washings, amniotic fluid) in analysis.

Handling Conditions

Check for sterility in sample transfer.
Use correct containers for testing.
Label samples correctly.
Follow sample transfer protocols.
Track samples accurately to designated labs.
If inadequate volume, communicate with caregiver.

Specimen Storage and Stability

Prompt examination prioritised for all fluids.
CSF samples are handled as STATs.
Perform examination within 2 hours of arrival; if >8 hours, add comment (SPEDL).
Fluid specimens stored for 7 days at 2-8°C.
Fluid cytosmear slides stored at least 1 week at room temperature.
Improper specimens noted in documentation.

QC Material

Sysmex XN Check BF - Automated Body Fluid Controls (Levels 1 & 2)
Do not freeze controls.
Store controls within recommended temperatures.
Discard vials if deteriorated.
Contact supervisor with deterioration suspicion.
Quantimetrix Spinalscopics – Manual Fluid Cell Count Controls (Levels 1 & 2)
Check for microbial contamination.
Use appropriate Cellpack dilutions for samples.

Performing QC - Sysmex XN Check BF

Remove XN Check BF control and equilibrate to 15-30°C for 15 minutes.
Analyze control in instrument QC Analysis mode.
Switch to Manual mode and select Body Fluid.
Wait for background check, print results, and file.
Mix vial, load Level 1 and Level 2 onto holder.
Process XN Check BF Level 2 after Level 1 status is green.

Performing QC - Quantimetrix Spinalscopics

Remove controls and let come to room temperature.
Mix controls thoroughly.
Fill hemacytometer chambers with controls
Count RBCs/WBCs.
Document control results.
If acceptability is not met, document troubleshooting and corrective action in Unity.

Performing QC - Background Checks

Review and find acceptable before use.
Document any corrective action needed in Unity.

Macroscopic Findings

Appearance: Color, clarity, and other attributes (whole specimen and supernatant)
Turbidity: (0–4+) for clarity
Consistency: Describing consistency of fluid

Procedure: Body Fluid Counts and Differentials, Automated and Manual

Include steps for preparation, specimen collection, and handling fluids.
Describe testing procedures and QC requirements.
Discuss microscopic findings and interpreting test results.
Ensure accuracy and consistency in performing tests and interpreting results.

Procedural Notes

Document abnormal CSF.
Explain causes for different body fluids.

Equipment and Materials

Sysmex XN Hematology Analyzer
Neubauer Hemacytometer/Disposable Hemacytometer
Microscope
Cyto-Tek Centrifuge
Cyto-Tek Specimen Chamber Assembly
Manual Cell Counter

Reagents

Cellpack - Diluting Fluid
Wright-Giemsa Stain Pack
New Methylene Blue
Hyaluronidase

Notes (regarding reagents)

Background checks performed on reagents.
Reagent stability periods documented.
Detailed information of reagent storage and use provided.

Reporting Automated Counts (from instrument)

Report results, including TNC-BF/WBC and RBC-BF, as whole numbers on worksheets.
Move decimal points for WBC/RBC reports (TNC-BF/WBC: 3 places to the right, RBC-BF=6 decimal places).
Document count in worksheets.

Studying That Suits You

Use AI to generate personalized quizzes and flashcards to suit your learning preferences.

Description

Test your knowledge on automated and manual methods for body fluid counts and differentials. This quiz covers essential concepts and practices used in hematology laboratories. Perfect for laboratory directors and medical education coordinators looking to refresh their skills.