Podcast
Questions and Answers
What should be done if deterioration of the Sysmex XN Check BF control is suspected?
What should be done if deterioration of the Sysmex XN Check BF control is suspected?
- Call the Sysmex Technical Assistance Center and inform the department supervisor. (correct)
- Discard the control vial immediately without further checks.
- Store the control at room temperature to prevent further damage.
- Continue using the control until results are received.
Which of the following is NOT a recommended practice for the Sysmex XN Check BF controls?
Which of the following is NOT a recommended practice for the Sysmex XN Check BF controls?
- Storing the controls in frozen conditions. (correct)
- Using controls only within their expected assay ranges.
- Maintaining the recommended temperature range for storage.
- Discarding vials confirmed to be damaged.
What action should be taken if a control vial of Quantimetrix Spinalscopics shows signs of microbial contamination?
What action should be taken if a control vial of Quantimetrix Spinalscopics shows signs of microbial contamination?
- Notify the supervisor but continue using the vial.
- Use the vial after sterilizing it.
- Retest the control before deciding on discarding it.
- Discard the control vial immediately. (correct)
What is indicated by the mean of a control result being outside the expected assay ranges?
What is indicated by the mean of a control result being outside the expected assay ranges?
Which of the following statements about automated and manual fluid control levels is correct?
Which of the following statements about automated and manual fluid control levels is correct?
What is the effective date of the current version 8.0 of the Body Fluid Counts and Differentials procedure?
What is the effective date of the current version 8.0 of the Body Fluid Counts and Differentials procedure?
Which of the following steps is NOT included in routine body fluid analysis?
Which of the following steps is NOT included in routine body fluid analysis?
What must be used if similar results are not produced when running specimens in duplicate?
What must be used if similar results are not produced when running specimens in duplicate?
What is the status of version 7.0 of the procedure?
What is the status of version 7.0 of the procedure?
For which type of analysis are validated fluid sample types analyzed on Sysmex XN Hematology analyzers?
For which type of analysis are validated fluid sample types analyzed on Sysmex XN Hematology analyzers?
Which device is utilized for manual counts of lower cell counts?
Which device is utilized for manual counts of lower cell counts?
What type of staining is used for manual differential counts?
What type of staining is used for manual differential counts?
Which of the following represents the timeline for version 8.0?
Which of the following represents the timeline for version 8.0?
When performing a 100 cell differential, what should you do if it is not possible?
When performing a 100 cell differential, what should you do if it is not possible?
What is the primary function of the MEM in reporting results?
What is the primary function of the MEM in reporting results?
What is required for slides showing abnormal results?
What is required for slides showing abnormal results?
What is indicated if results are abnormal or concerning during reporting?
What is indicated if results are abnormal or concerning during reporting?
What fluid types are mentioned that might require a differential count?
What fluid types are mentioned that might require a differential count?
What should be done with the Path Review form after completion?
What should be done with the Path Review form after completion?
What action is NOT recommended before completing a fluid differential?
What action is NOT recommended before completing a fluid differential?
What is the first step in performing a 100 cell differential?
What is the first step in performing a 100 cell differential?
What is the depth of the cell counting chamber in a hemacytometer?
What is the depth of the cell counting chamber in a hemacytometer?
What is the volume of the sample chamber in a hemacytometer?
What is the volume of the sample chamber in a hemacytometer?
Which ruling pattern is used in the metallized hemacytometer?
Which ruling pattern is used in the metallized hemacytometer?
How many small squares are in each group within the hemacytometer's central square millimeter?
How many small squares are in each group within the hemacytometer's central square millimeter?
What is the total area covered by the rulings in a hemacytometer?
What is the total area covered by the rulings in a hemacytometer?
Which of the following descriptions correctly identifies the groups in the hemacytometer's ruling?
Which of the following descriptions correctly identifies the groups in the hemacytometer's ruling?
What is indicated by the ruling lines in a hemacytometer?
What is indicated by the ruling lines in a hemacytometer?
When does the specified version of the Body Fluid Counts and Differentials procedures become effective?
When does the specified version of the Body Fluid Counts and Differentials procedures become effective?
What is the minimum number of white cells required in CSF to prepare a cytosmear slide?
What is the minimum number of white cells required in CSF to prepare a cytosmear slide?
What type of stain is used for staining the slide after smear preparation?
What type of stain is used for staining the slide after smear preparation?
Which fluid appearance requires using 1-3 drops for slide preparation?
Which fluid appearance requires using 1-3 drops for slide preparation?
When preparing a cytosmear slide, what is added to the sample chamber to potentially reduce cell distortion?
When preparing a cytosmear slide, what is added to the sample chamber to potentially reduce cell distortion?
At what relative centrifugal force (RCF) should the cytocentrifuge be set for processing the fluid?
At what relative centrifugal force (RCF) should the cytocentrifuge be set for processing the fluid?
What should be labeled on the cytosmear slide?
What should be labeled on the cytosmear slide?
If the fluid is bloody, how should it be diluted for slide preparation?
If the fluid is bloody, how should it be diluted for slide preparation?
What action must be taken with the sample chamber after it is filled?
What action must be taken with the sample chamber after it is filled?
Which of the following fluid types is classified under the LIS code 'PTDFL'?
Which of the following fluid types is classified under the LIS code 'PTDFL'?
What is the LIS code for colorless fluids?
What is the LIS code for colorless fluids?
Which site is responsible for all fluid types according to the provided content?
Which site is responsible for all fluid types according to the provided content?
Which fluid type corresponds to the LIS code 'SYN'?
Which fluid type corresponds to the LIS code 'SYN'?
What color does the LIS code 'AMBER' represent?
What color does the LIS code 'AMBER' represent?
Which of the following is NOT a type of body fluid mentioned?
Which of the following is NOT a type of body fluid mentioned?
What fluid type is represented by the LIS code 'JP'?
What fluid type is represented by the LIS code 'JP'?
The LIS code 'GRN' corresponds to which color of fluid?
The LIS code 'GRN' corresponds to which color of fluid?
Which site is primarily used for spinal fluids?
Which site is primarily used for spinal fluids?
What type of fluid is associated with the LIS code 'PFLD'?
What type of fluid is associated with the LIS code 'PFLD'?
Which LIS code should be used if a code isn't available for a fluid type?
Which LIS code should be used if a code isn't available for a fluid type?
What is the LIS code for pleural fluids?
What is the LIS code for pleural fluids?
Which color of fluid is represented by the LIS code 'PINK'?
Which color of fluid is represented by the LIS code 'PINK'?
For which type of fluid is the LIS code 'BROWN' used?
For which type of fluid is the LIS code 'BROWN' used?
Flashcards
Sysmex XN Check BF
Sysmex XN Check BF
A specialized type of control material used for validating the accuracy and precision of automated body fluid analysis systems.
Levels 1 and 2
Levels 1 and 2
Levels 1 and 2 refer to the different concentrations of analytes within the control material, allowing for assessment of both high and low ranges of measurement.
Storage Temperature
Storage Temperature
Storing controls outside the recommended temperature range can lead to degradation, affecting the reliability of test results.
Control Deterioration
Control Deterioration
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Quantimetrix Spinalscopics
Quantimetrix Spinalscopics
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Major revision
Major revision
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Approved and Current
Approved and Current
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Retired
Retired
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Date Added
Date Added
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Date Effective
Date Effective
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Date Retired
Date Retired
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Body Fluid Analysis
Body Fluid Analysis
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Sysmex XN Hematology Analyzer
Sysmex XN Hematology Analyzer
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Fluid Differential
Fluid Differential
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Fluid Type
Fluid Type
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100 Cell Differential
100 Cell Differential
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Adjusted Differential Count
Adjusted Differential Count
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Pathologist Review
Pathologist Review
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Reference Ranges
Reference Ranges
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LIS Reporting
LIS Reporting
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Hemacytometer
Hemacytometer
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Hausser Bright-Line Hemacytometer
Hausser Bright-Line Hemacytometer
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Cell Depth
Cell Depth
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Volume
Volume
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Ruling Pattern
Ruling Pattern
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Neubauer Ruling
Neubauer Ruling
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Boundary Lines
Boundary Lines
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Triple Lines
Triple Lines
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Cytosmear slide preparation
Cytosmear slide preparation
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Cytocentrifugation
Cytocentrifugation
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Wright's stain
Wright's stain
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Fluid volume for cytosmear preparation
Fluid volume for cytosmear preparation
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Bovine Serum Albumin (BSA)
Bovine Serum Albumin (BSA)
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Centrifugation speed adjustment
Centrifugation speed adjustment
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Slide preservation
Slide preservation
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Slide labeling
Slide labeling
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Pericardial Fluid
Pericardial Fluid
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Peritoneal Fluid
Peritoneal Fluid
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Pleural Fluid
Pleural Fluid
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Bronchial Washing Fluid
Bronchial Washing Fluid
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Jackson Pratt Fluid
Jackson Pratt Fluid
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Amniotic Fluid
Amniotic Fluid
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Cerebral Spinal Fluid
Cerebral Spinal Fluid
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Paracentesis Fluid
Paracentesis Fluid
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Peritoneal Dialysis Fluid
Peritoneal Dialysis Fluid
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Synovial Fluid
Synovial Fluid
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Fluid Sample
Fluid Sample
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Body Fluid Counts and Differentials
Body Fluid Counts and Differentials
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Fluid Color
Fluid Color
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Colorless Fluid
Colorless Fluid
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Red Fluid
Red Fluid
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Study Notes
Document Title and Number
- Title: Body Fluid Counts and Differentials – Automated and Manual
- Number: HEME-0001
Revision and Approval
- Revision: 8.0
- Approval Date: 6/12/2024
- Approved By: Clifton Bridges Laboratory Director SMH RGH (CLBRIDGE)
- Reviewed By: Clifton Bridges Laboratory Director SMH RGH (CLBRIDGE)
Review and Completion Dates
- Last Approval or Periodic Review Completed: 6/12/2024
- Next Periodic Review Needed On or Before: 6/12/2025
- Review Date: 6/12/2024
- Effective Date: 6/12/2024
Performed By
- Performed By: Clifton Bridges Laboratory Director SMH RGH (CLBRIDGE), Mark Williams Laboratory Director (WILL1050), Jack Wilson Laboratory Director SPEC RCH (JJWILSON), Angie Duong Laboratory Director (ADUONG), Jeffery Green MD (JTGREEN), Lindsey Matthews Laboratory Director (MATT0083), Cora G Uram-Tuculescu Laboratory Director (URAM0000), Thuy Sandy Pham Education Coordinator (101706)
Printed By
- Printed By: Leetta Bateman Lead MLS (12/12/2024)
Organization
- Organization: Richmond Market
Specimen Collection
- CSF: Submitted in 3 sterile tubes (labeled sequentially) - Tube 1: Chemical & Immunologic studies, Tube 2: Microbiologic examination, Tube 3: Cell Count and Cytological examination
- Other fluids (pleural, peritoneal, pericardial): Collected in EDTA tube or non-additive container (3-7mL volume ideal)
- Crystal analysis: Red top tube or non-additive container; avoid EDTA
- If EDTA is the only option for analysis, note.
- Include body fluids from other sites (e.g., bronchial washings, amniotic fluid) in analysis.
Handling Conditions
- Check for sterility in sample transfer.
- Use correct containers for testing.
- Label samples correctly.
- Follow sample transfer protocols.
- Track samples accurately to designated labs.
- If inadequate volume, communicate with caregiver.
Specimen Storage and Stability
- Prompt examination prioritised for all fluids.
- CSF samples are handled as STATs.
- Perform examination within 2 hours of arrival; if >8 hours, add comment (SPEDL).
- Fluid specimens stored for 7 days at 2-8°C.
- Fluid cytosmear slides stored at least 1 week at room temperature.
- Improper specimens noted in documentation.
QC Material
- Sysmex XN Check BF - Automated Body Fluid Controls (Levels 1 & 2)
- Do not freeze controls.
- Store controls within recommended temperatures.
- Discard vials if deteriorated.
- Contact supervisor with deterioration suspicion.
- Quantimetrix Spinalscopics – Manual Fluid Cell Count Controls (Levels 1 & 2)
- Check for microbial contamination.
- Use appropriate Cellpack dilutions for samples.
Performing QC - Sysmex XN Check BF
- Remove XN Check BF control and equilibrate to 15-30°C for 15 minutes.
- Analyze control in instrument QC Analysis mode.
- Switch to Manual mode and select Body Fluid.
- Wait for background check, print results, and file.
- Mix vial, load Level 1 and Level 2 onto holder.
- Process XN Check BF Level 2 after Level 1 status is green.
Performing QC - Quantimetrix Spinalscopics
- Remove controls and let come to room temperature.
- Mix controls thoroughly.
- Fill hemacytometer chambers with controls
- Count RBCs/WBCs.
- Document control results.
- If acceptability is not met, document troubleshooting and corrective action in Unity.
Performing QC - Background Checks
- Review and find acceptable before use.
- Document any corrective action needed in Unity.
Macroscopic Findings
- Appearance: Color, clarity, and other attributes (whole specimen and supernatant)
- Turbidity: (0–4+) for clarity
- Consistency: Describing consistency of fluid
Procedure: Body Fluid Counts and Differentials, Automated and Manual
- Include steps for preparation, specimen collection, and handling fluids.
- Describe testing procedures and QC requirements.
- Discuss microscopic findings and interpreting test results.
- Ensure accuracy and consistency in performing tests and interpreting results.
Procedural Notes
- Document abnormal CSF.
- Explain causes for different body fluids.
Equipment and Materials
- Sysmex XN Hematology Analyzer
- Neubauer Hemacytometer/Disposable Hemacytometer
- Microscope
- Cyto-Tek Centrifuge
- Cyto-Tek Specimen Chamber Assembly
- Manual Cell Counter
Reagents
- Cellpack - Diluting Fluid
- Wright-Giemsa Stain Pack
- New Methylene Blue
- Hyaluronidase
Notes (regarding reagents)
- Background checks performed on reagents.
- Reagent stability periods documented.
- Detailed information of reagent storage and use provided.
Reporting Automated Counts (from instrument)
- Report results, including TNC-BF/WBC and RBC-BF, as whole numbers on worksheets.
- Move decimal points for WBC/RBC reports (TNC-BF/WBC: 3 places to the right, RBC-BF=6 decimal places).
- Document count in worksheets.
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