Blood Specimen Types and Collection Methods

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17 Questions

What does the Nitroprusside test detect?

Acetoacetate

Which test is recommended for detecting dyslipidemia as a risk factor for Type 2 DM?

C-Peptide test

Why is OGTT not recommended for non-pregnant adults?

It is not accurate for non-pregnant adults

What does HBA1c reflect in terms of blood glucose levels?

Glucose levels over the past 2-3 months

Why is it important to properly prepare a patient before blood collection?

To ensure accurate test results

What happens if the plasma/serum is not separated from the red top or red cells for a period of time?

Glucose level increases

What is the difference in estimated glucose levels if yellow top is used instead of red top for testing?

12% lower in yellow top

How long is the glucose level stable at room temperature according to the text?

8 hours

Which chemical method involves the reduction of cupric ions to cuprous ions by glucose in hot alkaline conditions?

Alkaline Copper Reduction Method

What is the chromogen used in the Folin-Wu Method for glucose measurement?

Phosphomolybdate

Which method uses Alkaline Ferric Reduction for glucose measurement?

Benedict’s Method

Which method is the most specific and only reacts with beta-d-glucosidase?

Glucose Oxidase Method

In the context of glucose measurement, which method uses a toxic and corrosive agent, making it not commonly done?

Ortho-Toluidine Method

Which method is considered the reference method and measures NADH at 340 nm?

Hexokinase Method

Which enzyme-based method is derived from bacteria (Bacillus cereus) and highly specific for glucose?

Glucose Dehydrogenase

Which glucose measurement method involves the rate of disappearance of oxygen?

Glucose Oxidase Method

Which non-enzymatic method for glucose is considered to be the most specific?

Ortho-Toluidine Method

Learn about the types of blood specimens used in chemistry, such as red top and yellow top tubes, and the importance of separating plasma/serum from red cells. Discover how the choice of blood collection tube can affect glucose level measurement accuracy. Explore different methods of blood collection and the stability of collected blood samples.

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