Blood Specimen Collection Guidelines
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Questions and Answers

What is the primary reason for collecting blood specimens in the correct order of draw?

  • To avoid cross-contamination between different tube additives. (correct)
  • To reduce the time taken during the blood collection process.
  • To ensure that samples can be inverted multiple times.
  • To simplify the labeling process for each tube.

    • Which tube color requires the highest number of inversions after collection?

  • Red Top (plastic)
  • Gray Top (correct)
  • Light Blue Top
  • Lavender/Purple/Pink Top

    • What should be checked against a patient's identification bracelet after labeling a blood collection tube?

  • The tube color and type.
  • The collection site's location.
  • The patient's age and weight.
  • The labeled information on the tube. (correct)

    • Which of the following tubes is considered as the first tube to be collected when adhering to the order of draw?

    <p>Blood culture tube.</p> Signup and view all the answers

    Which statement accurately describes a non-additive blood collection tube?

    <p>Is represented by the Red Top (glass) tube and requires no inversions.</p> Signup and view all the answers

    What anticoagulant does the Pink Top tube contain for blood bank studies?

    <p>K2 EDTA.</p> Signup and view all the answers

    Which blood collection tube color is typically used for coagulation tests?

    <p>Light Blue Top.</p> Signup and view all the answers

    What is one of the risks associated with using plastic serum tubes that contain clot activators?

    <p>They interfere with coagulation testing.</p> Signup and view all the answers

    What could cause a falsely high RBC count?

    <p>Hemolysis (in vitro)</p> Signup and view all the answers

    Which type of error can lead to a falsely low MCH value?

    <p>Cold agglutinins</p> Signup and view all the answers

    What is a probable reason for a falsely high Mean Cell Volume (MCV)?

    <p>Autoagglutination</p> Signup and view all the answers

    Which parameter is falsely low due to hemolysis in vitro?

    <p>Hematocrit</p> Signup and view all the answers

    Which of the following can lead to a falsely low Mean Cell Hemoglobin Concentration (MCHC)?

    <p>Autoagglutination</p> Signup and view all the answers

    What probable reason could result in a falsely high hematocrit reading?

    <p>Hemoconcentration</p> Signup and view all the answers

    Which type of error can contribute to a falsely high Mean Cell Hemoglobin (MCH)?

    <p>Lipidemia</p> Signup and view all the answers

    What is a probable reason for falsely low hematocrit readings?

    <p>Improper sealing of the capillary tube</p> Signup and view all the answers

    What could cause a falsely high reading in Hemoglobin testing?

    <p>Autoagglutination</p> Signup and view all the answers

    Which condition is likely to cause a falsely low WBC count?

    <p>Clotting in sample</p> Signup and view all the answers

    What is a potential reason for a falsely elevated Platelet Count?

    <p>Autoagglutination</p> Signup and view all the answers

    Which of the following could influence the Mean Platelet Volume (MPV) to yield a falsely high result?

    <p>Determining MPV too early in an EDTA specimen</p> Signup and view all the answers

    Which factor is most likely to produce a falsely low reading in Lymphocyte count?

    <p>Large reactive lymphocytes</p> Signup and view all the answers

    What is a common cause for falsely low Neutrophil readings?

    <p>Neutrophil aggregation</p> Signup and view all the answers

    In which scenario would Eosinophil counts likely be falsely elevated?

    <p>Infection from microorganisms</p> Signup and view all the answers

    Which factor is linked to a falsely high Neutrophil count?

    <p>Hyperglycemia above 600 mg/dL</p> Signup and view all the answers

    What is the primary action that must be taken when a critical value is obtained?

    <p>Communicate with the responsible healthcare worker or physician.</p> Signup and view all the answers

    Which of the following critical value thresholds is correctly stated?

    <p>Platelets &lt; 25,000/uL</p> Signup and view all the answers

    What should be done if platelet satellitosis is observed during an EDTA blood smear?

    <p>Recollect a sample using a different anticoagulant.</p> Signup and view all the answers

    How is the correction for falsely low platelet counts computed when platelet satellitosis occurs?

    <p>PC × 1.1</p> Signup and view all the answers

    What is one of the benefits of using EDTA for blood smears?

    <p>It allows for multiple blood smears to be prepared.</p> Signup and view all the answers

    What is the main issue that resulted in the patient's initial low platelet count in the case study?

    <p>Presence of EDTA-induced platelet clumping.</p> Signup and view all the answers

    Which statement accurately describes a common misunderstanding about pseudothrombocytopenia?

    <p>It leads to falsely low platelet counts due to clumping in EDTA.</p> Signup and view all the answers

    What adjustment should be made to accurately retest the platelet and leukocyte counts following pseudothrombocytopenia?

    <p>Recollect using 3.2% NaCl and retest PC and WBC count.</p> Signup and view all the answers

    Which outcome does not correctly reflect the expected results after using the 3.2% sodium citrate tube?

    <p>Platelet count lower than 90,000/mm³.</p> Signup and view all the answers

    Which method is predominantly used in blood film preparation and involves a specific angle between slides?

    <p>Two-glass slide method (Manual Wedge Technique).</p> Signup and view all the answers

    Study Notes

    Reminders for Blood Specimen Collection

    • Collect blood specimens using the correct order of draw to prevent cross-contamination from tube additives.
    • Invert tubes containing additives immediately after collection.
    • Label tubes appropriately with patient's full name, unique identification number, date and time of collection, phlebotomist's initials, and compare with patient's identification bracelet.

    Order of Blood Draw

    • Blood culture tube (Yellow Top) - 8 inversions
    • Citrated tube (Light Blue Top) - 3-4 inversions
    • Serum tube (Red Top glass) - 0 inversions
    • Serum tube (Red Top plastic) - 5 inversions
    • Heparinized tube (Green Top) - 8 inversions
    • EDTA tube (Lavender/Purple/Pink Top) - 8 inversions
    • Sodium fluoride tube (Gray Top) - 8 inversions

    Other Blood Collection Tubes

    • Black Top: Contains 3.8% sodium citrate, blood to anticoagulant ratio 4:1, used for Westergren ESR.
    • Pink Top: Requires a special label, contains K2 EDTA, and can be used for a, 3.2% sodium citrate, or b, Citrate, Theophylline, Adenosine, Dipyridamole. Use cases include Blood Bank Studies and Whole blood Hematologic determinations.
    • Light Blue Top: may contain either 3.2% sodium citrate or Citrate, Theophylline, Adenosine, Dipyridamole. Uses include coagulation tests, platelet factor 4, and beta-thromboglobulin assays, and in Gray platelet syndrome.

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    Description

    This quiz focuses on the essential procedures for blood specimen collection, including the correct order of draw and proper labeling techniques. Participants will learn about different blood collection tubes and their uses to ensure accurate results and patient safety.

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