Blood Specimen Collection Guidelines

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Questions and Answers

What is the primary reason for collecting blood specimens in the correct order of draw?

  • To avoid cross-contamination between different tube additives. (correct)
  • To reduce the time taken during the blood collection process.
  • To ensure that samples can be inverted multiple times.
  • To simplify the labeling process for each tube.

Which tube color requires the highest number of inversions after collection?

  • Red Top (plastic)
  • Gray Top (correct)
  • Light Blue Top
  • Lavender/Purple/Pink Top

What should be checked against a patient's identification bracelet after labeling a blood collection tube?

  • The tube color and type.
  • The collection site's location.
  • The patient's age and weight.
  • The labeled information on the tube. (correct)

Which of the following tubes is considered as the first tube to be collected when adhering to the order of draw?

<p>Blood culture tube. (B)</p> Signup and view all the answers

Which statement accurately describes a non-additive blood collection tube?

<p>Is represented by the Red Top (glass) tube and requires no inversions. (C)</p> Signup and view all the answers

What anticoagulant does the Pink Top tube contain for blood bank studies?

<p>K2 EDTA. (A)</p> Signup and view all the answers

Which blood collection tube color is typically used for coagulation tests?

<p>Light Blue Top. (D)</p> Signup and view all the answers

What is one of the risks associated with using plastic serum tubes that contain clot activators?

<p>They interfere with coagulation testing. (B)</p> Signup and view all the answers

What could cause a falsely high RBC count?

<p>Hemolysis (in vitro) (C), Cryoglobulin (D)</p> Signup and view all the answers

Which type of error can lead to a falsely low MCH value?

<p>Cold agglutinins (A)</p> Signup and view all the answers

What is a probable reason for a falsely high Mean Cell Volume (MCV)?

<p>Autoagglutination (C)</p> Signup and view all the answers

Which parameter is falsely low due to hemolysis in vitro?

<p>Hematocrit (B), Mean Cell Hemoglobin Concentration (MCHC) (C), RBC count (D)</p> Signup and view all the answers

Which of the following can lead to a falsely low Mean Cell Hemoglobin Concentration (MCHC)?

<p>Autoagglutination (D)</p> Signup and view all the answers

What probable reason could result in a falsely high hematocrit reading?

<p>Hemoconcentration (C)</p> Signup and view all the answers

Which type of error can contribute to a falsely high Mean Cell Hemoglobin (MCH)?

<p>Lipidemia (C)</p> Signup and view all the answers

What is a probable reason for falsely low hematocrit readings?

<p>Improper sealing of the capillary tube (A)</p> Signup and view all the answers

What could cause a falsely high reading in Hemoglobin testing?

<p>Autoagglutination (B)</p> Signup and view all the answers

Which condition is likely to cause a falsely low WBC count?

<p>Clotting in sample (B)</p> Signup and view all the answers

What is a potential reason for a falsely elevated Platelet Count?

<p>Autoagglutination (B)</p> Signup and view all the answers

Which of the following could influence the Mean Platelet Volume (MPV) to yield a falsely high result?

<p>Determining MPV too early in an EDTA specimen (C)</p> Signup and view all the answers

Which factor is most likely to produce a falsely low reading in Lymphocyte count?

<p>Large reactive lymphocytes (A)</p> Signup and view all the answers

What is a common cause for falsely low Neutrophil readings?

<p>Neutrophil aggregation (D)</p> Signup and view all the answers

In which scenario would Eosinophil counts likely be falsely elevated?

<p>Infection from microorganisms (A)</p> Signup and view all the answers

Which factor is linked to a falsely high Neutrophil count?

<p>Hyperglycemia above 600 mg/dL (B)</p> Signup and view all the answers

What is the primary action that must be taken when a critical value is obtained?

<p>Communicate with the responsible healthcare worker or physician. (B)</p> Signup and view all the answers

Which of the following critical value thresholds is correctly stated?

<p>Platelets &lt; 25,000/uL (B)</p> Signup and view all the answers

What should be done if platelet satellitosis is observed during an EDTA blood smear?

<p>Recollect a sample using a different anticoagulant. (C)</p> Signup and view all the answers

How is the correction for falsely low platelet counts computed when platelet satellitosis occurs?

<p>PC × 1.1 (C)</p> Signup and view all the answers

What is one of the benefits of using EDTA for blood smears?

<p>It allows for multiple blood smears to be prepared. (D)</p> Signup and view all the answers

What is the main issue that resulted in the patient's initial low platelet count in the case study?

<p>Presence of EDTA-induced platelet clumping. (C)</p> Signup and view all the answers

Which statement accurately describes a common misunderstanding about pseudothrombocytopenia?

<p>It leads to falsely low platelet counts due to clumping in EDTA. (B)</p> Signup and view all the answers

What adjustment should be made to accurately retest the platelet and leukocyte counts following pseudothrombocytopenia?

<p>Recollect using 3.2% NaCl and retest PC and WBC count. (C)</p> Signup and view all the answers

Which outcome does not correctly reflect the expected results after using the 3.2% sodium citrate tube?

<p>Platelet count lower than 90,000/mm³. (B)</p> Signup and view all the answers

Which method is predominantly used in blood film preparation and involves a specific angle between slides?

<p>Two-glass slide method (Manual Wedge Technique). (C)</p> Signup and view all the answers

Flashcards

Blood Collection Order

Specific sequence for drawing blood samples to avoid contamination between different specimens and potential additive carryover.

Order of draw

The recommended sequence for collecting blood samples to reduce contamination and ensure accurate test results.

Additive carryover

Contamination of a blood sample with substances from a previous tube, leading to inaccurate lab results.

Blood culture tube

Tube specifically designed to grow microorganisms from a blood sample.

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Citrated tube

Tube containing anticoagulant citrate to prevent blood clotting during testing.

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Serum tube (non-additive)

Tube used to obtain serum (the liquid part of the blood after clotting). No anticoagulant.

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Heparinized tube

Tube using heparin as an anticoagulant, appropriate for testing where clotting is a problem.

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EDTA tube

Tube containing EDTA, an anticoagulant preserving cellular components like for blood counts efficiently.

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Sodium Fluoride tube

Tube containing fluoride preservative, important for tests that require glucose measurements to be precise and reliable.

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Gray Top Tube

Tube with gray color containing specific anticoagulant used for glucose measurements.

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Tube Inversion

Mixing the blood sample with the anticoagulant in the tube by inverting.

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Labeling Standards

The standardized procedure of labeling blood tubes including patient data (name, ID), date/time, and collector's initials.

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Falsely High RBC Count

A laboratory result showing a higher-than-actual red blood cell count due to factors like high white blood cell count, large platelets, or blood clotting issues.

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Falsely Low RBC Count

A lab result showing a lower-than-actual red blood cell count due to conditions like abnormal blood proteins or blood breakdown.

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Falsely High MCV

An inflated Mean Cell Volume (MCV) blood test result, potentially due to conditions like blood clumping or high white blood cell counts.

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Falsely Low MCV

Indicates an abnormally low Mean Cell Volume (MCV) lab result potentially caused by conditions that shrink the size of red blood cells.

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Falsely High MCH

An inflated Mean Cell Hemoglobin (MCH) test result likely due to abnormal amounts of lipids, or high white blood cell counts.

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Falsely Low MCH

A measurement of Mean Cell Hemoglobin (MCH) being lower than expected, potentially due to conditions like blood breakdown or clotting.

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Falsely High MCHC

Lab result indicating a higher-than-actual Mean Cell Hemoglobin Concentration (MCHC) potentially due to blood breakdown or clotting.

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Falsely Low MCHC

Lab result showing a lower-than-normal Mean Cell Hemoglobin Concentration (MCHC), potentially caused by various conditions.

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Falsely Low Hematocrit

A hematocrit result lower than actual, possibly due to blood breakdown, improper tube sealing, or extra anticoagulant.

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Falsely High Hematocrit

A hematocrit test that appears higher than the actual value, potentially caused by dehydration or improper equipment use.

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Falsely High Hemoglobin

Hemoglobin test result higher than the actual value, due to factors like large platelets, high white blood cells, or specific protein types.

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Falsely Low Hemoglobin

Hemoglobin test result lower than the actual value, possibly caused by blood clotting problems or cell breakdown during testing.

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Falsely High WBC Count

White blood cell count appears higher than the actual count, potentially because of abnormal blood components and types.

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Falsely Low WBC Count

White blood cell count appears lower than the actual count, maybe due to leukemia (especially during chemotherapy), or specific blood conditions.

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Falsely High Platelet Count

Platelet count appears higher than the actual value, potentially due to issues with certain blood cells or clotting problems.

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Falsely Low Platelet Count

Platelet count appears lower than the actual value, possible due to clotting problems, or blood cell clumps.

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Falsely High Neutrophils

Neutrophil count seems higher than actual, possibly due to neutrophil aggregation or them appearing as other cell types

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Falsely High Lymphocytes

Lymphocyte count is inaccurate, appearing higher than actual, potentially due to various cell types

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Critical Values

Blood test results that require immediate communication to the physician or healthcare worker.

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Hematocrit Critical Value

A hematocrit level of 50,000/uL in a new patient.

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Platelet Critical Value (low)

A platelet count below 20,000/uL, or if significantly higher than 1 million/uL and not reported before.

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Prothrombin Time Critical Value

A prothrombin time above 40 seconds.

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EDTA Blood Smear

Blood smear using EDTA to prevent platelet clumping, allowing for multiple smears and preparation later.

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Pseudothrombocytopenia

A falsely low platelet count due to platelet satellitosis (platelets sticking to white blood cells).

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Platelet Satellitosis

Platelets sticking to white blood cells, leading to a falsely low platelet count.

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Correction for Pseudothrombocytopenia

Recollect a new sample using 3.2% citrate, retest, compute corrected platelet count by multiplying by 1.1.

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EDTA-induced platelet clumping

Platelets clump together due to interactions with EDTA, sometimes making them appear as WBCs.

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Pseudothrombocytopenia

A falsely low platelet count caused by platelet clumping due to EDTA's effect.

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Platelet Clumping

Aggregation of platelets caused by certain anticoagulants, specifically EDTA.

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Pseudothrombocytopenia correction

Correcting a falsely low platelet count by collecting a new sample using 3.2% sodium citrate and correcting the counts

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Two-glass slide method

A common method for making blood smears by precisely angling two slides to create a thin, uniform smear for microscopy.

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Angle between slides (smear)

The appropriate angle (30-45 degrees) between slides when creating blood films.

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3.2% Sodium Citrate

Anticoagulant used to collect blood samples where platelet clumping is a concern, it's used to avoid pseudothrombocytopenia.

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Blood Film Preparation

Procedure used to create a thin, uniform blood smear for microscopic analysis, essential for identifying blood cells.

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Study Notes

Reminders for Blood Specimen Collection

  • Collect blood specimens using the correct order of draw to prevent cross-contamination from tube additives.
  • Invert tubes containing additives immediately after collection.
  • Label tubes appropriately with patient's full name, unique identification number, date and time of collection, phlebotomist's initials, and compare with patient's identification bracelet.

Order of Blood Draw

  • Blood culture tube (Yellow Top) - 8 inversions
  • Citrated tube (Light Blue Top) - 3-4 inversions
  • Serum tube (Red Top glass) - 0 inversions
  • Serum tube (Red Top plastic) - 5 inversions
  • Heparinized tube (Green Top) - 8 inversions
  • EDTA tube (Lavender/Purple/Pink Top) - 8 inversions
  • Sodium fluoride tube (Gray Top) - 8 inversions

Other Blood Collection Tubes

  • Black Top: Contains 3.8% sodium citrate, blood to anticoagulant ratio 4:1, used for Westergren ESR.
  • Pink Top: Requires a special label, contains K2 EDTA, and can be used for a, 3.2% sodium citrate, or b, Citrate, Theophylline, Adenosine, Dipyridamole. Use cases include Blood Bank Studies and Whole blood Hematologic determinations.
  • Light Blue Top: may contain either 3.2% sodium citrate or Citrate, Theophylline, Adenosine, Dipyridamole. Uses include coagulation tests, platelet factor 4, and beta-thromboglobulin assays, and in Gray platelet syndrome.

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