10 Questions
What angle should the spreader form with the slide?
40°
How should the smear be spread on the slide?
Gently by capillarity
How long should the diluted stain remain on the slide?
1–2 minutes
What should be done to dry the film on the slide?
Wave the slide in the air
What is a blood sample?
A small amount of blood obtained from a person for testing or investigations.
Who takes a blood sample?
A lab professional known as a phlebotomist.
What are the four most commonly used stains for blood smear?
Giemsa Stain, Leishman Stain, Wright's Stain, Jenner's Stain.
How is a blood film prepared?
Blood films can be made from anticoagulated or finger-prick blood.
What are the procedures for preparing a blood film from finger-prick blood?
- Keep the equipment ready. 2. Select a clean site. 3. Prepare the slides. 4. Use a sterile lancet to form a drop of blood on the fingertip. 5. Obtain free-flowing blood. 6. Do not press or squeeze the finger.
Why is it important for the selected site to be clean and free from infection?
To prevent contamination of the blood sample and reduce the risk of infection for the person being tested.
Study Notes
Blood Smear Preparation
- The spreader should form a 30-40° angle with the slide to create a smooth, even smear.
Staining a Blood Smear
- The diluted stain should remain on the slide for a specified time, usually 10-15 minutes.
- The film on the slide should be air-dried or dried with a cool air blower to prevent overheating, which can distort the blood cells.
Blood Sampling
- A blood sample is a specimen of blood collected for laboratory analysis.
- A phlebotomist or a medical professional trained in phlebotomy takes a blood sample.
- Blood samples can be collected through venipuncture or finger-prick methods.
Blood Film Preparation
- Four commonly used stains for blood smears are Giemsa, Wright, Jenner, and Leishman stains.
- A blood film is prepared by spreading a blood sample onto a slide, staining it, and then examining it under a microscope.
Finger-Prick Blood Film Preparation
- For preparing a blood film from finger-prick blood, the site should be cleaned and disinfected to prevent infection.
- The finger should be warmed to increase blood flow, and a small prick should be made with a sterile lancet.
Importance of Site Preparation
- It is crucial for the selected site to be clean and free from infection to prevent contamination and infection transmission during blood sampling.
Test your knowledge on the procedures and handling of blood samples for diagnostic purposes. Learn about the process of obtaining blood samples and the various tests conducted to examine its components.
Make Your Own Quizzes and Flashcards
Convert your notes into interactive study material.
Get started for free
