Podcast
Questions and Answers
What is the purpose of a blood sample?
What is the purpose of a blood sample?
Who is responsible for taking a blood sample?
Who is responsible for taking a blood sample?
Which stain is commonly used for staining blood films?
Which stain is commonly used for staining blood films?
What should be done to the lancet for finger puncture?
What should be done to the lancet for finger puncture?
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Why should the selected site for finger puncture be clean and free from infection, edema, or skin disease?
Why should the selected site for finger puncture be clean and free from infection, edema, or skin disease?
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What is the purpose of making blood films from anticoagulated or finger-prick blood?
What is the purpose of making blood films from anticoagulated or finger-prick blood?
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What should you do after allowing the blood drop to fall on the slide?
What should you do after allowing the blood drop to fall on the slide?
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What should you do with the spreader in step 8?
What should you do with the spreader in step 8?
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What is the purpose of dropping the diluted stain after drying the film?
What is the purpose of dropping the diluted stain after drying the film?
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What will happen if there is hesitation while spreading the smear?
What will happen if there is hesitation while spreading the smear?
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Study Notes
Blood Sample Purpose and Collection
- Blood samples are crucial for diagnosing medical conditions, monitoring health, and conducting research.
- A trained healthcare professional, such as a phlebotomist or nurse, is responsible for collecting blood samples.
Blood Film Staining
- The most commonly used stain for blood films is Giemsa stain, known for its ability to differentiate cellular components.
Finger Puncture Preparation
- Before using a lancet for finger puncture, it should be sterilized to prevent contamination and infection.
Site Preparation for Finger Puncture
- The selected site for finger puncture must be clean and free from infection, edema, or skin disease to ensure accurate results and minimize complications.
Purpose of Making Blood Films
- Making blood films from anticoagulated or finger-prick blood allows for examination of blood cells under a microscope and identification of abnormalities.
Blood Drop Application
- After allowing a drop of blood to fall onto the slide, it should be carefully spread to create a thin film for optimal visualization.
Spreader Handling
- The spreader should be held at an angle to ensure an even smear; a steady hand is required to achieve a consistent thickness.
Staining Process
- Dropping diluted stain onto a dried blood film serves to color the cells, allowing for better visibility of their morphology and differential diagnosis.
Effects of Hesitation During Smear
- Hesitation while spreading the smear can lead to an uneven thickness, which may affect the examination and interpretation of the blood film results.
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Description
Test your knowledge about the process of obtaining and analyzing blood samples for diagnostic and prognostic purposes. Learn about the procedures, significance, and applications of blood tests in medical investigations.
