Blood Clotting Time Procedure

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18 Questions

What is the normal range for clot retraction?

48-64%

What is the computation formula for platelet estimate?

Cells/uL = Average no.of platelets x 20,000

Which method of determining clotting time has less contamination of plasma with tissue fluids?

Sabraze's Coagulation time using capillary tubes

What is the objective of Platelet Estimate mentioned in the text?

Platelet count determination

Which temperature is mentioned for placing the tube in the water bath during clotting?

37°C

What is the average volume of serum expressed as a percentage of the original volume of whole blood after clotting?

55%

Which component is bound by sodium citrate in the whole blood to prevent anticoagulation?

Calcium

What is the first step in the capillary method of clotting time?

Disinfect the area of puncture

What is the normal capillary clotting time in the slide or drop method?

2-4 minutes

In the capillary method of clotting time, when should the timer be started?

As soon as the second drop of blood appears

Which method uses a clean slide and is considered the simplest among all clotting time methods?

Slide or Drop Method

What does the plasma contain after centrifugation in Prothrombin Time (PT) - Quick Method?

Intrinsic coagulation factors except calcium and platelets

Which pathway of hemostasis does the prothrombin time (PT) evaluate?

Extrinsic and common pathways

What is the purpose of adding calcium, phospholipids, and an activator to plasma in the clotting process?

To activate factor VII

What initiates the activation of factor VII in the clotting process?

Tissue extract (Factors III)

Which test is used as a means for measuring the effect of anticoagulants, especially coumarin?

Prothrombin time (PT)

What converts fibrinogen into fibrin during the clotting process?

Activated thrombin

Which factors is the PT test dependent upon for monitoring anticoagulant therapy?

I, II, V, VII, and X

Learn the step-by-step procedure for determining blood clotting time using the slide or drop method, including disinfecting the area, puncturing to a specific depth, and observing fibrin strands. Test your knowledge on this important laboratory technique.

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