3 Questions
What is the length of the query sequence? How many homologous sequences are in the database search? What are the ranges in %ide, %cov, and E-value? Are there any structures known?
The length of the query sequence is 381 amino acids. The number of homologous sequences in the database search will depend on the specific search performed. The ranges in %ide (percentage of identical matches), %cov (percentage of query coverage), and E-value (expect value) will also depend on the specific search parameters. The presence of known structures can be determined by searching against the Protein Data Bank (PDB).
Get the sequence of the protein mptpA (Tyrosine phosphatase A) from Mycobacterium tuberculosis from Uniprot.
The sequence of the protein mptpA (Tyrosine phosphatase A) from Mycobacterium tuberculosis can be retrieved from the Uniprot database.
How many homologous sequences are found using the NR database and default parameters on the Blast home page?
The number of homologous sequences found using the NR database and default parameters can be determined by performing a search on the Blast home page (https://blast.ncbi.nlm.nih.gov/Blast.cgi).
Identify Protein and Organism using BLAST Program Learn how to use BLAST to identify a protein sequence and determine which organism it belongs to. Test your skills with a provided protein sequence and discover the power of the BLAST program.
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