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Questions and Answers
What is the primary reason for the decrease in streptomycin production after carbohydrate depletion?
What is the primary reason for the decrease in streptomycin production after carbohydrate depletion?
- Denaturation of proteins
- Inhibition of enzyme activity
- Lysis of fungal cells (correct)
- Disruption of cellular metabolism
Which of the following is NOT a method used for microbial biotransformation?
Which of the following is NOT a method used for microbial biotransformation?
- Crude extract (correct)
- Resting cells
- Growing cultures
- Immobilized enzymes
What is the primary role of enzymes in biotransformation?
What is the primary role of enzymes in biotransformation?
- To inhibit microbial growth
- To regulate gene expression
- To adsorb onto charcoal
- To catalyze chemical reactions (correct)
What is the purpose of adding the substrate in a concentrated form during biotransformation using growing cells?
What is the purpose of adding the substrate in a concentrated form during biotransformation using growing cells?
Which of the following is a characteristic of biotransformation?
Which of the following is a characteristic of biotransformation?
What is the purpose of immobilized cells or enzymes in biotransformation?
What is the purpose of immobilized cells or enzymes in biotransformation?
What is the primary advantage of using fungal spores in steroid transformation?
What is the primary advantage of using fungal spores in steroid transformation?
What is the result of incubating spores of Streptomyces griseus with chloramphenicol?
What is the result of incubating spores of Streptomyces griseus with chloramphenicol?
What is the optimal incubation time for maximum spore-catalyzed chloramphenicol transformation activity?
What is the optimal incubation time for maximum spore-catalyzed chloramphenicol transformation activity?
What is the effect of adding glucose or acetate to the spores during the biotransformation process?
What is the effect of adding glucose or acetate to the spores during the biotransformation process?
What is the enzyme involved in the biotransformation of natural penicillin G to semi-synthetic penicillins?
What is the enzyme involved in the biotransformation of natural penicillin G to semi-synthetic penicillins?
What is the primary application of microbial fermentation in the production of antibiotics?
What is the primary application of microbial fermentation in the production of antibiotics?
What is the purpose of adding phenyl-alanine or phenyl acetic acid as a side-chain to the fermentation medium?
What is the purpose of adding phenyl-alanine or phenyl acetic acid as a side-chain to the fermentation medium?
What is the primary reason for using Penicillium chrysogenum mutants instead of Penicillium notatum in penicillin production?
What is the primary reason for using Penicillium chrysogenum mutants instead of Penicillium notatum in penicillin production?
What is the role of lactose in the penicillin production medium?
What is the role of lactose in the penicillin production medium?
What is the function of CaCO3 and KH2PO4 in the penicillin production medium?
What is the function of CaCO3 and KH2PO4 in the penicillin production medium?
What is the significance of 6-APA in penicillin production?
What is the significance of 6-APA in penicillin production?
What is the difference between penicillin produced by Penicillium notatum and Penicillium chrysogenum?
What is the difference between penicillin produced by Penicillium notatum and Penicillium chrysogenum?
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