Biotechnology Overview and Tools
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Questions and Answers

What is biotechnology primarily concerned with?

  • The use of living organisms to develop products or processes (correct)
  • Creating artificial intelligence
  • Studying human behavior
  • The use of animals for research purposes
  • DNA ligases are enzymes that cut DNA at specific sequences.

    False

    What is the primary purpose of gel electrophoresis in DNA analysis?

    To separate DNA molecules by size

    The method used for amplifying DNA sequences is called ______.

    <p>Polymerase chain reaction (PCR)</p> Signup and view all the answers

    Match the following applications of biotechnology to their descriptions:

    <p>Red Biotechnology = Medical applications like vaccines and pharmaceuticals Green Biotechnology = Agricultural applications like transgenic plants Blue Biotechnology = Marine biotechnology applications Yellow Biotechnology = Food biotechnology processes</p> Signup and view all the answers

    Which enzyme protects DNA from cleavage or damage?

    <p>Methylases</p> Signup and view all the answers

    Cloning is exclusively a feature of green biotechnology.

    <p>False</p> Signup and view all the answers

    What is the function of plasmids in biotechnology?

    <p>To study antibacterial resistance and gene expression</p> Signup and view all the answers

    Restriction fragment length polymorphism (RFLP) is used to compare differences in ______ between individuals.

    <p>DNA fragments</p> Signup and view all the answers

    Which of the following is NOT an application of the Barcode of Life project?

    <p>Gene therapy</p> Signup and view all the answers

    Study Notes

    Biotechnology Overview

    • Biotechnology uses living organisms or substances from living organisms to create agricultural, medicinal, or environmental products or processes.

    Tools in Biotechnology

    • Restriction endonucleases: Enzymes that cut DNA at specific sequences. Insertion of new sequences isn't typical, unlike CRISPR.
    • Methylases: Enzymes protecting DNA from cleavage or damage.
    • DNA ligases: Enzymes joining DNA fragments together.
    • Gel electrophoresis: Separates DNA molecules by size, used for analysis, fingerprinting, and paternity testing.
    • Plasmids: Circular DNA in bacteria. Scientists use them to study antibiotic resistance and gene expression.
    • Polymerase Chain Reaction (PCR): Amplifies DNA sequences, producing multiple copies for testing and research.
    • DNA sequencing: Used in projects like the Human Genome Project and the Barcode of Life, determining the order of DNA bases.

    Applications of Biotechnology

    • Red biotechnology: Medical applications, like producing antibiotics, vaccines, pharmaceuticals, genetic screening, genetic testing, gene therapy, and cloning.
    • Green biotechnology: Applied to agricultural processes, including creating transgenic plants (with genes from other organisms) to increase crop yields, nutrition, pest resistance, and environmental stress tolerance. Specific examples include soybeans, canola, cotton, and corn.
    • White biotechnology: Related to industrial processes, such as bioremediation (using microorganisms to clean contaminated soil or water) and using enzymes as catalysts for producing or destroying chemicals.
    • Blue biotechnology: Applied to marine and aquatic processes, aiming to increase seafood supply and safety, control noxious aquatic organisms, and develop new drugs.
    • Other examples: Artificial selection, artificial insemination, brewing, fermentation, gene splicing, stem cell and tissue regeneration, DNA testing, genomic sequencing, protein purification, and microbial synthetic biology.

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    8.1 Intro to Biotech PDF

    Description

    This quiz explores the essentials of biotechnology, including various tools and applications. You'll learn about restriction endonucleases, DNA ligases, PCR, and more, alongside their role in agriculture, medicine, and environmental conservation. Test your knowledge of how these powerful tools are applied in real-world scenarios.

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