Biotechnology Explorer ELISA Quiz
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Questions and Answers

What is the basic principle behind ELISpot?

  • Detection of infectious diseases
  • Using a washer system
  • Reading ELISA plates
  • Counting spot forming cells (correct)
  • What is the material used for most ELISA plates?

  • Aluminum
  • Polystyrene (correct)
  • Plastic
  • Glass
  • How many wells do 96-well ELISA plates typically have?

  • 8
  • 12
  • 6
  • 96 (correct)
  • What is the volume each well of an ELISA plate can hold?

    <p>350 μl</p> Signup and view all the answers

    Which type of plates are available for high throughput screening in ELISA?

    <p>384-well plates</p> Signup and view all the answers

    What is the main advantage of ELISA in terms of sensitivity?

    <p>High sensitivity</p> Signup and view all the answers

    What is the purpose of Northern blotting?

    <p>Detect the expression level and transcript size of a specific gene</p> Signup and view all the answers

    What is a disadvantage of the standard northern blot method?

    <p>Laborious if many genes need to be tested</p> Signup and view all the answers

    Which technique is used to analyze protein post translational modifications?

    <p>Eastern blotting</p> Signup and view all the answers

    What happens when RNA samples are degraded by RNAses in Northern blotting?

    <p>Quality of data and quantitation of expression are negatively affected</p> Signup and view all the answers

    Why does the use of radioactivity in Northern blotting pose a challenge?

    <p>Laborious if many genes need to be tested</p> Signup and view all the answers

    What is the main application of Northern blotting?

    <p>Detect mRNA expression levels and transcript sizes</p> Signup and view all the answers

    Which technique is suitable for detecting carbohydrate epitopes?

    <p>Eastern blotting</p> Signup and view all the answers

    What is a drawback of using the standard northern blot method with multiple probes?

    <p>Difficulty in signal detection</p> Signup and view all the answers

    Why is the standard northern blot method less sensitive than nuclease protection assays and RT-PCR?

    <p>Its sensitivity is not as high as other techniques</p> Signup and view all the answers

    What happens if RNA samples are slightly degraded during northern blot analysis?

    <p>The data and quantitation of expression may be negatively affected</p> Signup and view all the answers

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