Biotechnology Explorer ELISA Quiz

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What is the basic principle behind ELISpot?

Detection of infectious diseases

Using a washer system

Reading ELISA plates

Counting spot forming cells (correct)

What is the material used for most ELISA plates?

Aluminum

Polystyrene (correct)

Plastic

Glass

How many wells do 96-well ELISA plates typically have?

8

12

6

96 (correct)

What is the volume each well of an ELISA plate can hold?

350 μl Signup and view all the answers

Which type of plates are available for high throughput screening in ELISA?

384-well plates Signup and view all the answers

What is the main advantage of ELISA in terms of sensitivity?

High sensitivity Signup and view all the answers

What is the purpose of Northern blotting?

Detect the expression level and transcript size of a specific gene Signup and view all the answers

What is a disadvantage of the standard northern blot method?

Laborious if many genes need to be tested Signup and view all the answers

Which technique is used to analyze protein post translational modifications?

Eastern blotting Signup and view all the answers

What happens when RNA samples are degraded by RNAses in Northern blotting?

Quality of data and quantitation of expression are negatively affected Signup and view all the answers

Why does the use of radioactivity in Northern blotting pose a challenge?

Laborious if many genes need to be tested Signup and view all the answers

What is the main application of Northern blotting?

Detect mRNA expression levels and transcript sizes Signup and view all the answers

Which technique is suitable for detecting carbohydrate epitopes?

Eastern blotting Signup and view all the answers

What is a drawback of using the standard northern blot method with multiple probes?

Difficulty in signal detection Signup and view all the answers

Why is the standard northern blot method less sensitive than nuclease protection assays and RT-PCR?

Its sensitivity is not as high as other techniques Signup and view all the answers

What happens if RNA samples are slightly degraded during northern blot analysis?

The data and quantitation of expression may be negatively affected Signup and view all the answers

Biotechnology Explorer ELISA Quiz

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