Podcast
Questions and Answers
What is the basic principle behind ELISpot?
What is the basic principle behind ELISpot?
- Detection of infectious diseases
- Using a washer system
- Reading ELISA plates
- Counting spot forming cells (correct)
What is the material used for most ELISA plates?
What is the material used for most ELISA plates?
- Aluminum
- Polystyrene (correct)
- Plastic
- Glass
How many wells do 96-well ELISA plates typically have?
How many wells do 96-well ELISA plates typically have?
- 8
- 12
- 6
- 96 (correct)
What is the volume each well of an ELISA plate can hold?
What is the volume each well of an ELISA plate can hold?
Which type of plates are available for high throughput screening in ELISA?
Which type of plates are available for high throughput screening in ELISA?
What is the main advantage of ELISA in terms of sensitivity?
What is the main advantage of ELISA in terms of sensitivity?
What is the purpose of Northern blotting?
What is the purpose of Northern blotting?
What is a disadvantage of the standard northern blot method?
What is a disadvantage of the standard northern blot method?
Which technique is used to analyze protein post translational modifications?
Which technique is used to analyze protein post translational modifications?
What happens when RNA samples are degraded by RNAses in Northern blotting?
What happens when RNA samples are degraded by RNAses in Northern blotting?
Why does the use of radioactivity in Northern blotting pose a challenge?
Why does the use of radioactivity in Northern blotting pose a challenge?
What is the main application of Northern blotting?
What is the main application of Northern blotting?
Which technique is suitable for detecting carbohydrate epitopes?
Which technique is suitable for detecting carbohydrate epitopes?
What is a drawback of using the standard northern blot method with multiple probes?
What is a drawback of using the standard northern blot method with multiple probes?
Why is the standard northern blot method less sensitive than nuclease protection assays and RT-PCR?
Why is the standard northern blot method less sensitive than nuclease protection assays and RT-PCR?
What happens if RNA samples are slightly degraded during northern blot analysis?
What happens if RNA samples are slightly degraded during northern blot analysis?
