16 Questions
What is the basic principle behind ELISpot?
Counting spot forming cells
What is the material used for most ELISA plates?
Polystyrene
How many wells do 96-well ELISA plates typically have?
96
What is the volume each well of an ELISA plate can hold?
350 μl
Which type of plates are available for high throughput screening in ELISA?
384-well plates
What is the main advantage of ELISA in terms of sensitivity?
High sensitivity
What is the purpose of Northern blotting?
Detect the expression level and transcript size of a specific gene
What is a disadvantage of the standard northern blot method?
Laborious if many genes need to be tested
Which technique is used to analyze protein post translational modifications?
Eastern blotting
What happens when RNA samples are degraded by RNAses in Northern blotting?
Quality of data and quantitation of expression are negatively affected
Why does the use of radioactivity in Northern blotting pose a challenge?
Laborious if many genes need to be tested
What is the main application of Northern blotting?
Detect mRNA expression levels and transcript sizes
Which technique is suitable for detecting carbohydrate epitopes?
Eastern blotting
What is a drawback of using the standard northern blot method with multiple probes?
Difficulty in signal detection
Why is the standard northern blot method less sensitive than nuclease protection assays and RT-PCR?
Its sensitivity is not as high as other techniques
What happens if RNA samples are slightly degraded during northern blot analysis?
The data and quantitation of expression may be negatively affected
Test your knowledge on ELISA (Enzyme-Linked Immunosorbent Assay) with this quiz. Explore topics such as antibody structure, principle of ELISA, and detecting specific proteins in samples.
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