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Questions and Answers
______ stands for Enzyme-linked ImmunoSorbent Assay. Enzyme-Linked: enzyme amplifies antigen-antibody interaction ImmunoSorbent: reaction products adsorbed on container Assay: assessment of potential analyte ______: is an immunochemical assay that employs an enzyme linked to either anti-immunoglobulin or antibody specific for antigen and detects either antibody or antigen in a sample.
ELISA
The ______ has been used as a diagnostic tool in medicine and plant pathology, as well as a quality-control check in various industries.
ELISA
Components of ELISA Well: may be coated with known antibody or known antigen. Sample: unknown antibody or unknown antigen. Conjugate: is enzyme - labeled antibody ______: – Horseradish Peroxidase (HRP): is a more commonly used enzyme. A.
Enzyme
At the end of this lecture students should be able to … Understand the concept of ______ Describe types of ______ Be familiar with ______ device and apparatus Clinical application of ______ Perform ______ test and Interpreting the results What is an ______.
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______ ‫زانكويا ثوليتةكنيكى يا دهؤك‬ MSc. ‫دهــوك التقــنية‬ ‫ جامعـة‬In Immunology Duhok Polytechnic University
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Study Notes
ELISA (Enzyme-Linked ImmunoSorbent Assay)
- ELISA is an immunochemical assay that employs an enzyme linked to either anti-immunoglobulin or antibody specific for antigen
- It detects either antibody or antigen in a sample
Components of ELISA
- Well: may be coated with known antibody or known antigen
- Sample: unknown antibody or unknown antigen
- Conjugate: enzyme-labeled antibody
Enzymes Used in ELISA
- Horseradish Peroxidase (HRP): a commonly used enzyme
Learning Objectives
- Understand the concept of ELISA
- Describe types of ELISA
- Be familiar with ELISA device and apparatus
- Apply ELISA test and interpret the results
- Define what is an ELISA
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Description
Test your knowledge of ELISA (Enzyme Linked Immunosorbent Assay) and practical immunology with this quiz. Assess how well you understand the concepts taught by Dr. Amir Hani Raziq and Assistant Lecturer Hadi H. Hamad at Duhok Polytechnic University.
