ELISA Practical Immunology Lecture Quiz
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Questions and Answers

______ stands for Enzyme-linked ImmunoSorbent Assay. Enzyme-Linked: enzyme amplifies antigen-antibody interaction ImmunoSorbent: reaction products adsorbed on container Assay: assessment of potential analyte ______: is an immunochemical assay that employs an enzyme linked to either anti-immunoglobulin or antibody specific for antigen and detects either antibody or antigen in a sample.

ELISA

The ______ has been used as a diagnostic tool in medicine and plant pathology, as well as a quality-control check in various industries.

ELISA

Components of ELISA Well: may be coated with known antibody or known antigen. Sample: unknown antibody or unknown antigen. Conjugate: is enzyme - labeled antibody ______: – Horseradish Peroxidase (HRP): is a more commonly used enzyme. A.

Enzyme

At the end of this lecture students should be able to … Understand the concept of ______ Describe types of ______ Be familiar with ______ device and apparatus Clinical application of ______ Perform ______ test and Interpreting the results What is an ______.

<p>ELISA</p> Signup and view all the answers

______ ‫زانكويا ثوليتةكنيكى يا دهؤك‬ MSc. ‫دهــوك التقــنية‬ ‫ جامعـة‬In Immunology Duhok Polytechnic University

<p>Hadi H. Hamad</p> Signup and view all the answers

Study Notes

ELISA (Enzyme-Linked ImmunoSorbent Assay)

  • ELISA is an immunochemical assay that employs an enzyme linked to either anti-immunoglobulin or antibody specific for antigen
  • It detects either antibody or antigen in a sample

Components of ELISA

  • Well: may be coated with known antibody or known antigen
  • Sample: unknown antibody or unknown antigen
  • Conjugate: enzyme-labeled antibody

Enzymes Used in ELISA

  • Horseradish Peroxidase (HRP): a commonly used enzyme

Learning Objectives

  • Understand the concept of ELISA
  • Describe types of ELISA
  • Be familiar with ELISA device and apparatus
  • Apply ELISA test and interpret the results
  • Define what is an ELISA

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Description

Test your knowledge of ELISA (Enzyme Linked Immunosorbent Assay) and practical immunology with this quiz. Assess how well you understand the concepts taught by Dr. Amir Hani Raziq and Assistant Lecturer Hadi H. Hamad at Duhok Polytechnic University.

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