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Questions and Answers
Biotechnology is a broad term that involves many different types of technology. In some cases it involves analyzing DNA (simply _____ for its presence or maybe the presence of certain _____ ) and in others it involves manipulating the DNA (_____ it, copying it, or _____ it into other organisms).
Biotechnology is a broad term that involves many different types of technology. In some cases it involves analyzing DNA (simply _____ for its presence or maybe the presence of certain _____ ) and in others it involves manipulating the DNA (_____ it, copying it, or _____ it into other organisms).
looking, genes, cutting, inserting
How is DNA studied? In many cases, first the DNA has to be _____ from a cell. This is known as DNA _____. Nucleated cells (cells with a nucleus) are subjected to a chemical treatment. This is usually a mild _____ that ruptures the cell, releasing the DNA.
How is DNA studied? In many cases, first the DNA has to be _____ from a cell. This is known as DNA _____. Nucleated cells (cells with a nucleus) are subjected to a chemical treatment. This is usually a mild _____ that ruptures the cell, releasing the DNA.
removed, extraction, detergent
Since all of the DNA in a human cell would extend to 6 feet in length, it has to be chemically _____ into more manageable lengths. Scissors are not used for this process, instead chemicals known as _____ _____ are used.
Since all of the DNA in a human cell would extend to 6 feet in length, it has to be chemically _____ into more manageable lengths. Scissors are not used for this process, instead chemicals known as _____ _____ are used.
cut, restriction, enzymes
Once the DNA is cut, many things may be done. In forensics investigations, the DNA is separated and analyzed for _____. This performed with a process called _____ _____. The DNA fragments are placed on a gel and then an electric current is passed through. The smaller fragments travel _____ and further than the larger fragments and this produces a pattern on the gel. Everyone's DNA will produce different _____ . This is called a _____ _____.
Once the DNA is cut, many things may be done. In forensics investigations, the DNA is separated and analyzed for _____. This performed with a process called _____ _____. The DNA fragments are placed on a gel and then an electric current is passed through. The smaller fragments travel _____ and further than the larger fragments and this produces a pattern on the gel. Everyone's DNA will produce different _____ . This is called a _____ _____.
Since all of the DNA in a human cell would extend to 6 feet in length, it has to be chemically _____ into more manageable lengths. Scissors are not used for this process, instead chemicals known as _____ _____ are used. Restriction enzymes are very specific compounds that cut the DNA at predetermined locations.
Since all of the DNA in a human cell would extend to 6 feet in length, it has to be chemically _____ into more manageable lengths. Scissors are not used for this process, instead chemicals known as _____ _____ are used. Restriction enzymes are very specific compounds that cut the DNA at predetermined locations.
Once the DNA is cut, many things may be done. In forensics investigations, the DNA is separated and analyzed for _____. This performed with a process called - The DNA fragments are placed on a gel and then an electric current is passed through. The smaller fragments travel _____ than the larger fragments and this produces a pattern on the gel. Everyone's DNA will produce different _____ This is called a _____ _____.
Once the DNA is cut, many things may be done. In forensics investigations, the DNA is separated and analyzed for _____. This performed with a process called - The DNA fragments are placed on a gel and then an electric current is passed through. The smaller fragments travel _____ than the larger fragments and this produces a pattern on the gel. Everyone's DNA will produce different _____ This is called a _____ _____.
Flashcards
Biotechnology
Biotechnology
Analyzing or manipulating DNA for various applications.
DNA Extraction
DNA Extraction
Initial step in DNA study; removing it from cells.
Restriction Enzymes
Restriction Enzymes
Enzymes that cut DNA at specific sequences.
Gel Electrophoresis
Gel Electrophoresis
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DNA Fingerprint
DNA Fingerprint
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Why is DNA removed from cells?
Why is DNA removed from cells?
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Why must DNA be cut?
Why must DNA be cut?
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How do restriction enzymes work?
How do restriction enzymes work?
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How do detergents help in DNA study?
How do detergents help in DNA study?
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How does DNA fragment size affect movement in gel electrophoresis?
How does DNA fragment size affect movement in gel electrophoresis?
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Study Notes
- Biotechnology broadly involves different types of technology.
- Biotechnology involves analyzing DNA to look for the presence of certain genes.
- Biotechnology manipulates DNA by cutting, copying, or inserting it into other organisms.
DNA Study
- DNA needs to be removed from a cell.
- DNA extraction is subjecting nucleated cells (cells with a nucleus) to a mild detergent.
- Detergent ruptures the cell, releasing the DNA.
- DNA has to be chemically cut into more manageable lengths, because all of the DNA in a human cell would extend to six feet in length.
- Scissors are not used for cutting.
- Restriction enzymes are used.
- Restriction enzymes are very specific compounds that cut the DNA at predetermined locations.
- The restriction enzyme EcoRI finds the sequence GAATTC in DNA and performs a chemical cut wherever this sequence occurs.
- Restriction enzymes perform their cuts after different patterns.
DNA Analysis
- After the DNA is cut, DNA is separated and analyzed for patterns.
- The process is called gel electrophoresis.
- DNA fragments are placed on gel and then an electric current is passed through.
- Smaller fragments travel faster and further than larger fragments.
- Different patterns are produced on the gel.
- Everyone's DNA will produce different patterns, called a DNA fingerprint.
DNA Fingerprints
- Unknown blood stain is compared to possible suspects.
- The pattern of the DNA only matches one individual.
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