Podcast
Questions and Answers
What approach utilizes the analysis of genomes present in different environments to identify various molecules?
What approach utilizes the analysis of genomes present in different environments to identify various molecules?
- Metagenomics (correct)
- Proteomics
- Mutant selection
- Strain preservation
What is the commonly maintained CO2 level in incubators for animal cell culture?
What is the commonly maintained CO2 level in incubators for animal cell culture?
- 11-15%
- 3-4%
- 1-2%
- 5-10% (correct)
What factors contribute to the challenge of accurately counting genes in a genome?
What factors contribute to the challenge of accurately counting genes in a genome?
- (iii), (iv)
- (i), (iii)
- (ii), (iv)
- (i), (ii) (correct)
To prevent ripening of avocados during shipping, which type of gene should be introduced?
To prevent ripening of avocados during shipping, which type of gene should be introduced?
Which of the following is a microbial species used for commercial production of citric acid?
Which of the following is a microbial species used for commercial production of citric acid?
Which process would most likely enhance the genetic stability of a cultivar?
Which process would most likely enhance the genetic stability of a cultivar?
In biotechnology, which of the following techniques is crucial for separating DNA fragments?
In biotechnology, which of the following techniques is crucial for separating DNA fragments?
What is the role of adjuvants in vaccine formulations?
What is the role of adjuvants in vaccine formulations?
Which method helps reduce the possibility of religation of vector without picking the insert?
Which method helps reduce the possibility of religation of vector without picking the insert?
What does a yellow color spot indicate in a cDNA microarray experiment using red and green fluorescent dyes?
What does a yellow color spot indicate in a cDNA microarray experiment using red and green fluorescent dyes?
Which disease is associated with loci on chromosome number 1 and 6?
Which disease is associated with loci on chromosome number 1 and 6?
Which molecular disease causes increased hydrophobic interactions within the hemoglobin protein?
Which molecular disease causes increased hydrophobic interactions within the hemoglobin protein?
Interleukin-2 produced using CHO cell line is primarily used for which therapy?
Interleukin-2 produced using CHO cell line is primarily used for which therapy?
What contributes to the dynamic nature of a cell's proteome?
What contributes to the dynamic nature of a cell's proteome?
A novel gene introduced into yeast cells is used to produce which of the following?
A novel gene introduced into yeast cells is used to produce which of the following?
Which of the following techniques involves the use of hybridoma technology?
Which of the following techniques involves the use of hybridoma technology?
What is the molecular basis of sickle cell anaemia?
What is the molecular basis of sickle cell anaemia?
Why is autoclaving important for culturing microbes?
Why is autoclaving important for culturing microbes?
Which antibiotic is typically used for selecting recombinant cells in genetic engineering?
Which antibiotic is typically used for selecting recombinant cells in genetic engineering?
What is the function of chymotrypsin in the charge relay system?
What is the function of chymotrypsin in the charge relay system?
What could cause a recombinant polypeptide hormone to be inactive after purification?
What could cause a recombinant polypeptide hormone to be inactive after purification?
What is a potential solution to the extinction of medicinal plants?
What is a potential solution to the extinction of medicinal plants?
Which technique can be used to identify mutations linked to a disease?
Which technique can be used to identify mutations linked to a disease?
What is the role of taxol derived from Taxus spp. in medicine?
What is the role of taxol derived from Taxus spp. in medicine?
What is the first step involved in the isolation of a desired microbial product after fermentation?
What is the first step involved in the isolation of a desired microbial product after fermentation?
Why is cell disruption necessary when isolating intracellular metabolites?
Why is cell disruption necessary when isolating intracellular metabolites?
At which stage of isolation is the metabolite purified to a high degree?
At which stage of isolation is the metabolite purified to a high degree?
What is the final step in the isolation process for achieving nearly complete purity?
What is the final step in the isolation process for achieving nearly complete purity?
Which option describes the entire process of isolating a microbial product?
Which option describes the entire process of isolating a microbial product?
If a metabolite is extracellular, what step is preferred after separating the cells?
If a metabolite is extracellular, what step is preferred after separating the cells?
What could happen if there are too many steps in the isolation process?
What could happen if there are too many steps in the isolation process?
What type of product is purified during the metabolite-specific purification step?
What type of product is purified during the metabolite-specific purification step?
What is the correct relationship between the assertion that patients administered monoclonal antibodies against CD3 can accept renal allograft and the reason that monoclonal antibodies block T cell function?
What is the correct relationship between the assertion that patients administered monoclonal antibodies against CD3 can accept renal allograft and the reason that monoclonal antibodies block T cell function?
What causes foaming in microbiological cultures according to the reason provided?
What causes foaming in microbiological cultures according to the reason provided?
Why can yeast cells with YEp plasmid be selected on a medium lacking leucine?
Why can yeast cells with YEp plasmid be selected on a medium lacking leucine?
What are somaclonal variations associated with in long-term callus and cell suspension cultures?
What are somaclonal variations associated with in long-term callus and cell suspension cultures?
What is the maximum insert size for plasmid vectors according to the provided information?
What is the maximum insert size for plasmid vectors according to the provided information?
What is one important reason for fusing B lymphocytes with myeloma cells in the hybridoma technique?
What is one important reason for fusing B lymphocytes with myeloma cells in the hybridoma technique?
What makes 2D Gel Electrophoresis suitable for studying proteomics?
What makes 2D Gel Electrophoresis suitable for studying proteomics?
Why has sickle cell trait persisted in populations where malaria is endemic?
Why has sickle cell trait persisted in populations where malaria is endemic?
Study Notes
General Instructions for Biotechnology Exam
- Maximum Marks: 70, Time allowed: 3 hours
- All questions are compulsory
- Five sections comprising a total of 33 questions
- Section A: 12 multiple choice questions and 4 assertion-reasoning questions (1 mark each)
- Section B: 5 short answer questions (2 marks each)
- Section C: 7 short answer questions (3 marks each)
- Section D: 2 case-based questions (4 marks each)
- Section E: 3 long answer questions (5 marks each)
- No overall choice but internal choices available in some questions
- Diagrams should be neat and properly labeled when necessary
Section A: Multiple Choice Questions
- Metagenomics evaluates gene presence in various environments.
- CO2 levels in animal cell culture are typically maintained at 5-10%.
- Difficulties in counting genes arise from splice variants, overlapping genes, post-transcriptional events, and mutations.
- Genetic engineering in avocados to delay ripening can involve introducing specific genes like those from Bacillus amyloliquefaciens.
- Aspergillus niger is a key microbial species for commercial citric acid production.
- Methods to prevent religation of vectors without insert include using alkaline phosphatase and two different restriction enzymes.
- Yellow spots in a cDNA microarray signify equal gene expression in normal and diseased cells.
- Huntington's disease and migraine are associated with loci on chromosomes 1 and 6.
- Sickle cell anemia leads to increased hydrophobic interactions in hemoglobin.
- Interleukin-2 from CHO cells is mainly used in cancer therapy.
- The proteome is dynamic due to biochemical changes in response to environmental influences.
- Yeast cells engineered to produce hepatitis B vaccine induce specific immune responses.
Section B: Short Answer Questions
- Cloning vectors include plasmids (0.5-8 kb), cosmids (9-23 kb), and YACs (50-500 kb).
- Pathologists differentiate between cancerous and non-cancerous cells through microscopic observations of structural differences.
- Inverted microscopes are preferred for cell culture to accommodate dish access, while hybridoma techniques merge B lymphocytes with myeloma cells for monoclonal antibody production.
- 2D Gel Electrophoresis is suitable for proteomics as it separates proteins based on charge and molecular weight.
- The dbEST database is designed to handle redundancy in Expressed Sequence Tag (EST) data, while curators maintain data integrity and relevance.
Section C: Detailed Short Answer Questions
- Sickle cell trait provides malaria resistance; the mutation alters β-globin locus, differentiating normal from sickle cells.
- Nutrient media must be autoclaved to eliminate microbial contaminants; heat-sensitive antibiotics can be sterilized through filtration.
- Industrial metabolites like Vincristine (source: Vinca rosea), Quinine (source: Cinchona), and Taxol (source: Taxus brevifolia) have significant medicinal uses.
- The charge relay system in chymotrypsin utilizes two histidine residues for enzymatic activity.
- Cloned eukaryotic hormones may be inactive in bacteria due to lack of post-translational modifications.
- Steps in creating a chimeric mouse include isolation of embryonic stem cells, introduction of foreign DNA, and implantation in a blastocyst.
Section D: Case-Based Questions
- Identify specific mutations and diseases through affected gene and employed techniques for diagnosis.
- Downstream processing involves metabolite recovery and purification techniques to isolate microbial products post-fermentation.
Section E: Long Answer Questions
- Calculate m/z ratios for proteins based on charges and molecular weight; draw corresponding Mass spectrum.
- Consideration for protein-rich diets includes amino acid profiles, digestibility, and biological value for performance enhancement in athletes.
- Regeneration of plants from tissue culture involves steps from explant preparation to regeneration; applications include rapid propagation and genetic improvement.
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Description
Prepare for your Class XII Biotechnology exam with this sample question paper. It includes 33 questions covering multiple choice and short answer formats, designed to test your knowledge and understanding of key concepts in biotechnology. Use this paper to identify your strengths and weaknesses before the actual exam.
