Biotechnology Basics Quiz
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Questions and Answers

What is the purpose of using TTT in the process of cDNA synthesis?

  • To initiate the reverse transcriptase activity
  • To create a complementary strand of DNA (correct)
  • To act as a template for mRNA synthesis
  • To bind to the primer for reverse transcription
  • In cDNA synthesis, what is the role of RNase H?

  • To stabilize the mRNA template
  • To initiate the synthesis of cDNA
  • To prevent DNA degradation
  • To degrade the RNA in RNA-DNA hybrid (correct)
  • What is the function of alkaline hydrolysis in cDNA synthesis?

  • To degrade the mRNA (correct)
  • To remove RNA primers
  • To activate DNA polymerase
  • To separate DNA strands
  • What is the purpose of using oligo dG primer in cDNA synthesis?

    <p>To prime the synthesis of cDNA from the poly A tail of mRNA</p> Signup and view all the answers

    What is the role of terminal transferase (TTTT) in cDNA synthesis?

    <p>To add nucleotides to the 3' end of DNA fragments</p> Signup and view all the answers

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