5 Questions
What is the purpose of using TTT in the process of cDNA synthesis?
To create a complementary strand of DNA
In cDNA synthesis, what is the role of RNase H?
To degrade the RNA in RNA-DNA hybrid
What is the function of alkaline hydrolysis in cDNA synthesis?
To degrade the mRNA
What is the purpose of using oligo dG primer in cDNA synthesis?
To prime the synthesis of cDNA from the poly A tail of mRNA
What is the role of terminal transferase (TTTT) in cDNA synthesis?
To add nucleotides to the 3' end of DNA fragments
Test your knowledge of biotechnology basics with this quiz covering topics such as recombinant DNA technology, vector transformation, genomic libraries, screening, and prokaryotic and eukaryotic cells.
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