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Questions and Answers
What is the purpose of using TTT in the process of cDNA synthesis?
What is the purpose of using TTT in the process of cDNA synthesis?
- To initiate the reverse transcriptase activity
- To create a complementary strand of DNA (correct)
- To act as a template for mRNA synthesis
- To bind to the primer for reverse transcription
In cDNA synthesis, what is the role of RNase H?
In cDNA synthesis, what is the role of RNase H?
- To stabilize the mRNA template
- To initiate the synthesis of cDNA
- To prevent DNA degradation
- To degrade the RNA in RNA-DNA hybrid (correct)
What is the function of alkaline hydrolysis in cDNA synthesis?
What is the function of alkaline hydrolysis in cDNA synthesis?
- To degrade the mRNA (correct)
- To remove RNA primers
- To activate DNA polymerase
- To separate DNA strands
What is the purpose of using oligo dG primer in cDNA synthesis?
What is the purpose of using oligo dG primer in cDNA synthesis?
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What is the role of terminal transferase (TTTT) in cDNA synthesis?
What is the role of terminal transferase (TTTT) in cDNA synthesis?
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