Biotechnology and Plant Tissue Culture

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Questions and Answers

What is a primary advantage of plant tissue culture?

  • Elimination of the need for soil
  • Rapid multiplication of disease-free plants (correct)
  • Increased genetic diversity among plants
  • Decreased need for environmental control

Which of the following is a disadvantage of plant tissue culture?

  • Wide applicability for all plant species
  • High initial costs for setup (correct)
  • Requires minimal technical skills
  • Long process for plant maturation

What role do growth regulators play in plant tissue culture?

  • They prevent the growth of any plant tissues
  • They are used solely for rooting media
  • They control the growth and differentiation of plant cells (correct)
  • They enhance the genetic diversity of plant cultures

Which media component is typically responsible for supporting the growth of plant tissues in culture?

<p>Agar or gelatin (B)</p> Signup and view all the answers

What is a fundamental concept in increasing the production of pharmaceuticals through plant tissue culture?

<p>Optimizing culture conditions for specific metabolites (A)</p> Signup and view all the answers

Why is it important to record advances in plant biotechnology?

<p>To improve efficiency and stay updated in the field (B)</p> Signup and view all the answers

What is a common method for micropropagation in endangered plants?

<p>Using tissue culture techniques (B)</p> Signup and view all the answers

Which of the following statements best explains the term 'elicitation protocols' in natural product enhancement?

<p>They stimulate plant responses to enhance secondary metabolite production (C)</p> Signup and view all the answers

What is one of the primary advantages of plant tissue culture?

<p>Ability to propagate plants rapidly (D)</p> Signup and view all the answers

Which of the following describes a common problem associated with plant tissue culture?

<p>Formation of somaclonal variation (D)</p> Signup and view all the answers

What is typically included in the nutritional requirements for plant tissue culture?

<p>Minerals, vitamins, and hormones (C)</p> Signup and view all the answers

Which stage is NOT typically part of the plant tissue culture process?

<p>Implementation (A)</p> Signup and view all the answers

What is the primary goal of plant regeneration in tissue culture?

<p>To produce new plant varieties (A)</p> Signup and view all the answers

Which of the following factors can negatively affect plant tissue culture productivity?

<p>Contamination by microorganisms (C)</p> Signup and view all the answers

What is one method used to increase secondary metabolite production in plant tissue culture?

<p>Application of elicitors (B)</p> Signup and view all the answers

What is one of the primary principles of plant tissue culture?

<p>Isolation of plant cells (A)</p> Signup and view all the answers

Which is an advantage of plant tissue culture?

<p>Rapid propagation of disease-free plants (B)</p> Signup and view all the answers

A major disadvantage of plant tissue culture is its:

<p>Potential for low genetic variation (C)</p> Signup and view all the answers

During plant tissue culture, what is a crucial stage in the process?

<p>Sterilization of tools and media (B)</p> Signup and view all the answers

What is one factor that can limit the effectiveness of plant tissue culture?

<p>Lack of specialized skills in handling plant tissues (C)</p> Signup and view all the answers

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Study Notes

Biotechnology: Four Colors

  • Biotechnology is the application of biological principles to produce goods and services
  • Green biotechnology focuses on environmentally friendly solutions to low crop productivity caused by human activities
  • Red biotechnology focuses on medical applications, for example, producing pharmaceuticals
  • White biotechnology focuses on industrial applications, for example, bioremediating pollutants
  • Blue biotechnology focuses on marine applications, such as aquaculture and biofuel production

Plant Tissue Culture

  • Advantages:
    • Production of disease-free plants
    • Mass propagation
    • Conservation of endangered species
    • Production of valuable secondary metabolites
  • Disadvantages:
    • Labor-intensive
    • Sterile environment required
    • Expensive
  • Stages:
    • Initiation: Explant is sterilized and cultured on growth medium
    • Multiplication: Culture is maintained and propagated
    • Rooting: Roots develop
    • Acclimatization: Plants are transferred to greenhouse or field conditions

Biotechnological Production of Herbal Drugs

  • The course focuses on the scope and practice of plant biotechnology
  • Covers the basic principles of biotechnology and the four colors of biotechnology
  • Discusses downstream processes of plant cells and tissue culture
  • Examines genetic engineering techniques
  • Explores potential applications of biotechnology in the market

Plant Genetic Manipulation

  • Advantages of Genetically Modified (GM) Crops:
    • Increased yield
    • Pest resistance
    • Herbicide tolerance
    • Improved nutrient content
  • Disadvantages of Genetically Modified (GM) Crops:
    • Potential environmental risks, such as gene flow to non-GM crops
    • Potential human health risks, such as allergies
    • Concerns over corporate control of agriculture

Pharmaceutical Applications of Biotechnology

  • Plant biotechnology can be used to produce:
    • Pharmaceuticals, such as insulin and human growth hormone
    • Vaccines, such as the Hepatitis B vaccine
    • Diagnostic tools
    • Agricultural products, such as pest-resistant crops
  • Plant tissue culture has been used to produce valuable secondary metabolites, like:
    • Taxol for cancer treatment
    • Vincristine and vinblastine for leukemia treatment

Green Biotechnology

  • Green biotechnology aims to provide environmentally friendly solutions to low crop productivity
  • It can be used to create crops resistant to insects and pests, and with increased drought tolerance
  • Potential benefits include:
    • Increased crop yield
    • Reduced pesticide use
    • Improved nutritional value of crops
    • Enhanced durability of harvested products
  • Potential risks:
    • Gene transfer to other species
    • Allergic reactions
    • Socioeconomic consequences, such as unequal access to technology and resources

Midterm:

  • 30 marks

Semester Activity:

  • 10 marks for quizzes
  • 10 marks for activities & presentations
  • 5 marks for student attendance
  • 5 marks for student portfolio

Final Exam:

  • 40 marks

Total marks: 100

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