Podcast
Questions and Answers
What is the most commonly used vector for propagating recombinant DNA?
What is the most commonly used vector for propagating recombinant DNA?
- Biolistic particles
- Microinjection needles
- Plasmids (correct)
- CRISPR-Cas9 complexes
Which step in the recombinant DNA process involves 'joining' the gene of interest to the vector?
Which step in the recombinant DNA process involves 'joining' the gene of interest to the vector?
- Transfer of the Recombinant DNA into a host cell
- Cutting or Cleavage of DNA
- Selection of appropriate Vector
- Ligation (correct)
What is the purpose of the 'Selection Process' step in recombinant DNA technology?
What is the purpose of the 'Selection Process' step in recombinant DNA technology?
- To identify the gene or protein of interest
- To select the cells that contain the gene of interest (correct)
- To make copies of the recombined DNA
- To harvest the desired product
Which technique is used to directly deliver DNA into the nucleus of a cell?
Which technique is used to directly deliver DNA into the nucleus of a cell?
Which gene editing technology relies on the use of guide RNA to direct Cas9 endonuclease to a specific DNA sequence?
Which gene editing technology relies on the use of guide RNA to direct Cas9 endonuclease to a specific DNA sequence?
What is the process called when a bacterial plasmid is cut by restriction enzymes to make space for a gene of interest?
What is the process called when a bacterial plasmid is cut by restriction enzymes to make space for a gene of interest?
Which technique involves directly injecting genetic material into an organism's cells?
Which technique involves directly injecting genetic material into an organism's cells?
What is the main function of ligase in genetic engineering?
What is the main function of ligase in genetic engineering?
Which of the following is NOT a method of introducing foreign DNA into bacteria for genetic engineering?
Which of the following is NOT a method of introducing foreign DNA into bacteria for genetic engineering?
In the context of genetic engineering, what do vectors refer to?
In the context of genetic engineering, what do vectors refer to?
Which method involves coating tiny particles with DNA and shooting them directly into cells using a gun-like device?
Which method involves coating tiny particles with DNA and shooting them directly into cells using a gun-like device?
What is the main purpose of using vectors in biotechnology?
What is the main purpose of using vectors in biotechnology?
Which technique involves the introduction of rDNA into an organism using tiny particles shot from a gene gun?
Which technique involves the introduction of rDNA into an organism using tiny particles shot from a gene gun?
What is the primary role of CRISPR in genetic modification?
What is the primary role of CRISPR in genetic modification?
In the process of transformation in biotechnology, what is the function of vectors?
In the process of transformation in biotechnology, what is the function of vectors?
Which method involves the direct injection of genetic material into a cell using a fine needle under a microscope?
Which method involves the direct injection of genetic material into a cell using a fine needle under a microscope?
What is the purpose of heat shock treatment in genetic engineering?
What is the purpose of heat shock treatment in genetic engineering?
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