Biotechnology and Genetic Modification Quiz
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Questions and Answers

What is the most commonly used vector for propagating recombinant DNA?

  • Biolistic particles
  • Microinjection needles
  • Plasmids (correct)
  • CRISPR-Cas9 complexes
  • Which step in the recombinant DNA process involves 'joining' the gene of interest to the vector?

  • Transfer of the Recombinant DNA into a host cell
  • Cutting or Cleavage of DNA
  • Selection of appropriate Vector
  • Ligation (correct)
  • What is the purpose of the 'Selection Process' step in recombinant DNA technology?

  • To identify the gene or protein of interest
  • To select the cells that contain the gene of interest (correct)
  • To make copies of the recombined DNA
  • To harvest the desired product
  • Which technique is used to directly deliver DNA into the nucleus of a cell?

    <p>Microinjection</p> Signup and view all the answers

    Which gene editing technology relies on the use of guide RNA to direct Cas9 endonuclease to a specific DNA sequence?

    <p>CRISPR-Cas9</p> Signup and view all the answers

    What is the process called when a bacterial plasmid is cut by restriction enzymes to make space for a gene of interest?

    <p>Transformation</p> Signup and view all the answers

    Which technique involves directly injecting genetic material into an organism's cells?

    <p>CRISPR</p> Signup and view all the answers

    What is the main function of ligase in genetic engineering?

    <p>To insert genes into plasmids</p> Signup and view all the answers

    Which of the following is NOT a method of introducing foreign DNA into bacteria for genetic engineering?

    <p>Molecular Methods</p> Signup and view all the answers

    In the context of genetic engineering, what do vectors refer to?

    <p>DNA molecules that carry foreign genetic material into another cell</p> Signup and view all the answers

    Which method involves coating tiny particles with DNA and shooting them directly into cells using a gun-like device?

    <p>Biolistics</p> Signup and view all the answers

    What is the main purpose of using vectors in biotechnology?

    <p>Introducing new traits into an organism</p> Signup and view all the answers

    Which technique involves the introduction of rDNA into an organism using tiny particles shot from a gene gun?

    <p>Biolistics</p> Signup and view all the answers

    What is the primary role of CRISPR in genetic modification?

    <p>Cutting DNA at specific sites</p> Signup and view all the answers

    In the process of transformation in biotechnology, what is the function of vectors?

    <p>Delivering recombinant DNA into an organism</p> Signup and view all the answers

    Which method involves the direct injection of genetic material into a cell using a fine needle under a microscope?

    <p>Microinjection</p> Signup and view all the answers

    What is the purpose of heat shock treatment in genetic engineering?

    <p>Enhancing the efficiency of transformation</p> Signup and view all the answers

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