Podcast
Questions and Answers
What is a major advantage of hybridoma technology in antibody production?
What is a major advantage of hybridoma technology in antibody production?
- Labor-intensive animal maintenance
- Highly pure and specific antibodies (correct)
- Require multiple animal species for production
- Limited production of monoclonal antibodies
What is the primary target of Evolocumab in reducing LDL cholesterol levels?
What is the primary target of Evolocumab in reducing LDL cholesterol levels?
- Proprotein convertase subtilisin/kexin type 9 (PCSK9) (correct)
- LDL receptors on the liver surface
- Liver cells producing LDL cholesterol
- Low-density lipoprotein (LDL) in the blood
What is the effect of Evolocumab binding to PCSK9?
What is the effect of Evolocumab binding to PCSK9?
- Inhibits the production of LDL cholesterol in the liver
- Reduces the liver's ability to remove LDL cholesterol from the blood
- Increases the degradation of LDL receptors on the liver surface
- Enhances the removal of LDL cholesterol from the blood (correct)
What type of antibody is Evolocumab?
What type of antibody is Evolocumab?
What is the purpose of Evolocumab in patients with familial hypercholesterolemia?
What is the purpose of Evolocumab in patients with familial hypercholesterolemia?
What is the primary function of PCSK9 in the liver?
What is the primary function of PCSK9 in the liver?
What is the composition of the infliximab antibody?
What is the composition of the infliximab antibody?
What is the function of the glycosylation pattern in the Fc region of the infliximab antibody?
What is the function of the glycosylation pattern in the Fc region of the infliximab antibody?
How does infliximab's Fab region interact with TNF-α?
How does infliximab's Fab region interact with TNF-α?
What is the significance of the complementary determining regions (CDRs) in infliximab's therapeutic efficacy?
What is the significance of the complementary determining regions (CDRs) in infliximab's therapeutic efficacy?
What is the initial step in the production of infliximab antibodies?
What is the initial step in the production of infliximab antibodies?
What is the role of the adjuvant in the murine A2 hybridoma development?
What is the role of the adjuvant in the murine A2 hybridoma development?
What is the primary mechanism by which PEG modifications increase the half-life of antibodies?
What is the primary mechanism by which PEG modifications increase the half-life of antibodies?
What is the effect of glycans on antigenic epitopes in proteins?
What is the effect of glycans on antigenic epitopes in proteins?
What is the result of substitution mutations in the constant region of the antibody, such as T250Q/M428L or M252Y/S254T/T256E?
What is the result of substitution mutations in the constant region of the antibody, such as T250Q/M428L or M252Y/S254T/T256E?
What is the effect of decreasing the pI of the variable region on the clearance of IgG antibodies?
What is the effect of decreasing the pI of the variable region on the clearance of IgG antibodies?
What is the purpose of using only the Fab fragment of the antibody?
What is the purpose of using only the Fab fragment of the antibody?
What is the role of the neonatal Fc receptor (FcRn) in the clearance of IgG antibodies?
What is the role of the neonatal Fc receptor (FcRn) in the clearance of IgG antibodies?
What is a key difference in the production method of biosimilars compared to generic drugs?
What is a key difference in the production method of biosimilars compared to generic drugs?
What is the primary reason for the higher risk of immunogenicity in biosimilars compared to generic drugs?
What is the primary reason for the higher risk of immunogenicity in biosimilars compared to generic drugs?
What is the FDA requirement for biosimilar manufacturers?
What is the FDA requirement for biosimilar manufacturers?
What is a potential consequence of immunogenicity in biosimilars?
What is a potential consequence of immunogenicity in biosimilars?
What is a key difference between biosimilars and generic drugs in terms of their structural similarity to the reference product?
What is a key difference between biosimilars and generic drugs in terms of their structural similarity to the reference product?
Why is it important for clinicians to monitor and report suspected immunological responses to biosimilars?
Why is it important for clinicians to monitor and report suspected immunological responses to biosimilars?
What is a benefit of developing biosimilars compared to standard biologic development pathways?
What is a benefit of developing biosimilars compared to standard biologic development pathways?
What is the type of monoclonal antibody that Trastuzumab is?
What is the type of monoclonal antibody that Trastuzumab is?
Where is Trastuzumab produced?
Where is Trastuzumab produced?
What is the target of Trastuzumab?
What is the target of Trastuzumab?
What is the normal function of HER2?
What is the normal function of HER2?
What is the result of HER2 activation in breast cancer?
What is the result of HER2 activation in breast cancer?
