Biosafety Cabinet Best Practices Quiz

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Questions and Answers

Why are open flames not necessary within the clean environment of a Class II or III BSC?

  • They emit harmful radiation
  • They generate too much heat
  • They are too expensive to maintain
  • They cause disruption of the airflow inside (correct)

What is the recommended distance to keep clean materials from aerosol-generating activities inside a BSC?

  • 18 inches (45 cm)
  • 6 inches (15 cm)
  • 12 inches (30 cm) (correct)
  • 24 inches (60 cm)

What is the recommended procedure for arranging the work flow inside a BSC?

  • From contaminated to clean
  • Randomly
  • From high to low intensity activities
  • From clean to contaminated (correct)

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Study Notes

Biosafety Cabinets (BSCs)

  • Open flames are not necessary within the clean environment of a Class II or III BSC because they can create turbulence, disrupt airflow, and potentially contaminate the environment.
  • Clean materials should be kept at a recommended distance of at least 12 inches (30 cm) from aerosol-generating activities inside a BSC to prevent cross-contamination.
  • The recommended procedure for arranging the work flow inside a BSC is to work from clean to contaminated, following a unidirectional workflow, and keeping the most critical activities, such as inoculation, in the center of the cabinet.

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