Podcast
Questions and Answers
Why are open flames not necessary within the clean environment of a Class II or III BSC?
Why are open flames not necessary within the clean environment of a Class II or III BSC?
- They emit harmful radiation
- They generate too much heat
- They are too expensive to maintain
- They cause disruption of the airflow inside (correct)
What is the recommended distance to keep clean materials from aerosol-generating activities inside a BSC?
What is the recommended distance to keep clean materials from aerosol-generating activities inside a BSC?
- 18 inches (45 cm)
- 6 inches (15 cm)
- 12 inches (30 cm) (correct)
- 24 inches (60 cm)
What is the recommended procedure for arranging the work flow inside a BSC?
What is the recommended procedure for arranging the work flow inside a BSC?
- From contaminated to clean
- Randomly
- From high to low intensity activities
- From clean to contaminated (correct)
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Study Notes
Biosafety Cabinets (BSCs)
- Open flames are not necessary within the clean environment of a Class II or III BSC because they can create turbulence, disrupt airflow, and potentially contaminate the environment.
- Clean materials should be kept at a recommended distance of at least 12 inches (30 cm) from aerosol-generating activities inside a BSC to prevent cross-contamination.
- The recommended procedure for arranging the work flow inside a BSC is to work from clean to contaminated, following a unidirectional workflow, and keeping the most critical activities, such as inoculation, in the center of the cabinet.
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