3 Questions
Why are open flames not necessary within the clean environment of a Class II or III BSC?
They cause disruption of the airflow inside
What is the recommended distance to keep clean materials from aerosol-generating activities inside a BSC?
12 inches (30 cm)
What is the recommended procedure for arranging the work flow inside a BSC?
From clean to contaminated
Study Notes
Biosafety Cabinets (BSCs)
- Open flames are not necessary within the clean environment of a Class II or III BSC because they can create turbulence, disrupt airflow, and potentially contaminate the environment.
- Clean materials should be kept at a recommended distance of at least 12 inches (30 cm) from aerosol-generating activities inside a BSC to prevent cross-contamination.
- The recommended procedure for arranging the work flow inside a BSC is to work from clean to contaminated, following a unidirectional workflow, and keeping the most critical activities, such as inoculation, in the center of the cabinet.
"Test Your Knowledge on Best Practices for Working in a Biosafety Cabinet" - Take this quiz to assess your understanding of the recommended safety measures for working within a Biosafety Cabinet (BSC). Topics covered include reducing aerosol generation, proper material placement, and efficient workflow organization to avoid contamination and personnel exposure.
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