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Questions and Answers
What is the primary function of a laboratory bag mixer?
What is the primary function of a laboratory bag mixer?
- To blend solid samples for homogenization (correct)
- To store samples at low temperatures
- To dispense controlled amounts of solutions
- To count colonies on agar plates
Which statement about the plate centrifuge is correct?
Which statement about the plate centrifuge is correct?
- It prepares solid sample mixtures only
- It creates pellets by freezing samples
- It uses high temperatures for separation
- It separates denser substances by spinning them away from the central axis (correct)
What is the main purpose of using an ultra-low freezer in microbiology?
What is the main purpose of using an ultra-low freezer in microbiology?
- To incubate samples for rapid growth
- To sterilize equipment and materials
- To grow microbes at a controlled temperature
- For cryogenic storage of reference cultures at -80°C (correct)
How does the optical film function when used with well-plates?
How does the optical film function when used with well-plates?
What role does the colony counter play in microbiology laboratories?
What role does the colony counter play in microbiology laboratories?
Which of the following is NOT a common inoculating technique?
Which of the following is NOT a common inoculating technique?
What is the main function of an automatic dispenser in a laboratory?
What is the main function of an automatic dispenser in a laboratory?
What is the primary use of cryogenic storage vials in a laboratory?
What is the primary use of cryogenic storage vials in a laboratory?
What is the primary purpose of using an autoclave in a microbiology lab?
What is the primary purpose of using an autoclave in a microbiology lab?
Which setting on an autoclave is considered standard for effective sterilization?
Which setting on an autoclave is considered standard for effective sterilization?
What is the main function of a biosafety cabinet in a microbiology laboratory?
What is the main function of a biosafety cabinet in a microbiology laboratory?
What is an essential practice for maintaining sterility during lab procedures?
What is an essential practice for maintaining sterility during lab procedures?
How does an incubator typically maintain a constant temperature for samples?
How does an incubator typically maintain a constant temperature for samples?
Which of the following is NOT a guideline for the use of Personal Protective Equipment (PPE) in the lab?
Which of the following is NOT a guideline for the use of Personal Protective Equipment (PPE) in the lab?
What is an important aspect of maintaining microbiological cultures over time?
What is an important aspect of maintaining microbiological cultures over time?
Which statement about refrigeration in microbiology is true?
Which statement about refrigeration in microbiology is true?
What is the primary function of a Biosafety Cabinet (BSC)?
What is the primary function of a Biosafety Cabinet (BSC)?
What is a key feature of the air flow in a Biosafety Cabinet?
What is a key feature of the air flow in a Biosafety Cabinet?
Why should alcohol lamps not be used for prolonged periods inside a Biosafety Cabinet?
Why should alcohol lamps not be used for prolonged periods inside a Biosafety Cabinet?
What is the main purpose of incubators in microbiology?
What is the main purpose of incubators in microbiology?
What does annual calibration of incubators ensure?
What does annual calibration of incubators ensure?
How does a water bath function in microbiological procedures?
How does a water bath function in microbiological procedures?
What is one of the functions of a refrigerator in microbiological contexts?
What is one of the functions of a refrigerator in microbiological contexts?
What is the significance of having a HEPA filter in a Biosafety Cabinet?
What is the significance of having a HEPA filter in a Biosafety Cabinet?
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Study Notes
Biosafety Cabinet (BSC)
- Provides protection from contamination by external sources, preventing sample contamination.
- Does not prevent contamination by the analyst; practicing aseptic techniques is essential.
- Equipped with a HEPA filter to maintain sterile conditions.
- Has negative airflow to ensure contaminants are kept out, crucial for maintaining safety.
- Alcohol lamps should not be used inside BSCs to avoid disruption of airflow.
Incubator
- Used to maintain constant temperatures for sample incubation.
- Calibrated annually to ensure precise temperature settings.
- Plays a essential role in experimental consistency for microbiological studies.
Water Bath
- Similar to an incubator, but specialized for maintaining the temperature of agar media.
- Prevents agar from solidifying, allowing for effective use in experiments.
Refrigerator
- Essential for storing prepared media and reagents.
- Supports the maintenance of bacterial suspensions and cell storage.
- Comes in various types, optimizing storage conditions for different laboratory needs.
Good Laboratory Practices (GLP)
- Adheres to FDA regulations for laboratory safety and efficacy.
- Lab sections must be distinct to minimize cross-contamination and optimize workflow.
- Aseptic technique is crucial for maintaining sterility and preventing contamination.
- Personal Protective Equipment (PPE) guidelines must be followed to ensure safety in the lab.
- Best practices for maintaining microbiological cultures to ensure their integrity over time.
- Quality assurance processes include routine checks and proper documentation to uphold lab standards.
Autoclave
- Sterilization device set at 121°C, 15 minutes, and 1.5 psi for media and tools.
- Also used for decontamination settings, typically at 30 minutes.
- Employs steam under pressure to effectively kill bacteria.
Plate Centrifuge
- Designed for spinning down denser substances to separate them from liquids.
- Useful in RT-PCR protocols, facilitating quick processing in microplates.
- Generates a pellet of concentrated cells during centrifugation.
Laboratory Bag Mixer
- Functions as a homogenizer for preparing solid samples.
- Paddle blender ensures thorough mixing and preparation in sample and broth.
Automatic Dispenser
- Allows for controlled and adjustable dispensing of solutions, streamlining laboratory workflows.
Ultra-Low Freezer
- Critical for the storage of reference cultures at -80°C.
- Accommodates cryogenic storage vials for preserving biological material.
Colony Counter
- Used to count microbial colonies on agar plates, ensuring proper quantification.
- Manual counting options allow for flexibility in counting isolated or overlapping colonies.
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