Questions and Answers
Which step involves identifying hazards associated with an infectious agent or material?
What is the minimum time that 5.25% hypochlorite (bleach) should be in contact with a surface for effective decontamination?
When evaluating risk, which factor should be considered regarding laboratory staff?
Which of the following measures is NOT a routine agent for surface decontamination?
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What should be done before disposing of strong acids or bases?
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Which of the following pathogens does a 10% solution of household bleach inactivate in just 2 minutes?
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Which step in the risk assessment process involves the review of national and institutional regulations?
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What is the purpose of a biorisk assessment?
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Which of the following is an example of a physical decontamination method?
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In the risk assessment process, what does reviewing risks and risk control measures focus on?
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What is the correct disposal method for contaminated sharps like needles and scalpels?
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What must be done to contaminated material if it is to be reused or recycled?
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Which of the following materials can be reused or recycled without any prior treatment?
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What is the first step in disposing of contaminated material for disposal?
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What is the proper action for laboratory coats that have been in contact with infectious materials?
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What should happen to pipettes and glass objects before disposal?
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What type of waste must always be treated as infectious and handled accordingly?
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What must be done to contaminated material before it can be considered uncontaminated?
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Which of the following describes contaminated materials for disposal regarding their treatment?
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Study Notes
Biorisk Assessment Overview
- Biorisk assessment reviews laboratory practices, control measures, biosafety standards, and the properties of infectious agents.
- Aims to prevent laboratory-acquired infections (LAIs) and exposure to high-risk pathogens.
- Evaluates potential risks to laboratory personnel and possible outcomes of exposure.
- Provides strategies to mitigate identified risks, conducted by institutions periodically using standardized checklists.
Steps in Performing Risk Assessment
- Gather information on hazards associated with infectious agents or materials.
- Evaluate risks related to activities that may cause exposure to these agents.
- Develop a risk control strategy that considers staff competencies and resource availability.
- Select and implement measures that align with national and institutional regulations.
- Review and continuously assess risks and control measures to ensure effectiveness.
Common Agents for Surface Decontamination
- Effective decontamination agents include heat (250° C for 15 minutes), ethylene oxide, glutaraldehyde (2%), 10% hydrogen peroxide, and 5.25% hypochlorite (10% bleach).
- Household bleach can inactivate hepatitis B virus in 10 minutes and HIV in 2 minutes.
- Bleach should remain in contact with surfaces for at least 20 minutes for effective decontamination.
Disposal of Contaminated Waste
- Strong acids or bases must be neutralized prior to disposal; do not add water to concentrated acids.
- Personal protective equipment (PPE) that may contact infectious material must be removed before exiting the laboratory and should not leave the facility.
- Contaminated PPE must be properly disposed of, ensuring it does not leave the laboratory.
Treatment of Laboratory Waste
- Uncontaminated (Non-infectious) Material: Can be reused, recycled, or disposed of as general waste.
- Contaminated Sharps (e.g., needles, scalpels): Must be collected in puncture-proof containers and treated as infectious waste.
- Contaminated Material for Reuse: Should be decontaminated before washing and treating as non-infectious.
- Contaminated Material for Disposal: Requires decontamination on-site or safe storage before transportation for further decontamination and disposal.
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Description
This quiz covers the essential aspects of biorisk assessment, including laboratory practices, biosafety standards, and risk mitigation strategies. Learn how to evaluate and prevent laboratory-acquired infections (LAIs) and the importance of reviewing infectious agents' hazardous characteristics. Test your knowledge on best practices in laboratory safety and risk management.
