Podcast
Questions and Answers
Which step involves identifying hazards associated with an infectious agent or material?
Which step involves identifying hazards associated with an infectious agent or material?
- Evaluate the risk
- Gather information (correct)
- Select and implement risk control measures
- Develop a risk control strategy
What is the minimum time that 5.25% hypochlorite (bleach) should be in contact with a surface for effective decontamination?
What is the minimum time that 5.25% hypochlorite (bleach) should be in contact with a surface for effective decontamination?
- 5 minutes
- 20 minutes (correct)
- 15 minutes
- 10 minutes
When evaluating risk, which factor should be considered regarding laboratory staff?
When evaluating risk, which factor should be considered regarding laboratory staff?
- Competencies and experience (correct)
- Availability of safety equipment
- Location of the laboratory
- Type of infectious agent handled
Which of the following measures is NOT a routine agent for surface decontamination?
Which of the following measures is NOT a routine agent for surface decontamination?
What should be done before disposing of strong acids or bases?
What should be done before disposing of strong acids or bases?
Which of the following pathogens does a 10% solution of household bleach inactivate in just 2 minutes?
Which of the following pathogens does a 10% solution of household bleach inactivate in just 2 minutes?
Which step in the risk assessment process involves the review of national and institutional regulations?
Which step in the risk assessment process involves the review of national and institutional regulations?
What is the purpose of a biorisk assessment?
What is the purpose of a biorisk assessment?
Which of the following is an example of a physical decontamination method?
Which of the following is an example of a physical decontamination method?
In the risk assessment process, what does reviewing risks and risk control measures focus on?
In the risk assessment process, what does reviewing risks and risk control measures focus on?
What is the correct disposal method for contaminated sharps like needles and scalpels?
What is the correct disposal method for contaminated sharps like needles and scalpels?
What must be done to contaminated material if it is to be reused or recycled?
What must be done to contaminated material if it is to be reused or recycled?
Which of the following materials can be reused or recycled without any prior treatment?
Which of the following materials can be reused or recycled without any prior treatment?
What is the first step in disposing of contaminated material for disposal?
What is the first step in disposing of contaminated material for disposal?
What is the proper action for laboratory coats that have been in contact with infectious materials?
What is the proper action for laboratory coats that have been in contact with infectious materials?
What should happen to pipettes and glass objects before disposal?
What should happen to pipettes and glass objects before disposal?
What type of waste must always be treated as infectious and handled accordingly?
What type of waste must always be treated as infectious and handled accordingly?
What must be done to contaminated material before it can be considered uncontaminated?
What must be done to contaminated material before it can be considered uncontaminated?
Which of the following describes contaminated materials for disposal regarding their treatment?
Which of the following describes contaminated materials for disposal regarding their treatment?
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Study Notes
Biorisk Assessment Overview
- Biorisk assessment reviews laboratory practices, control measures, biosafety standards, and the properties of infectious agents.
- Aims to prevent laboratory-acquired infections (LAIs) and exposure to high-risk pathogens.
- Evaluates potential risks to laboratory personnel and possible outcomes of exposure.
- Provides strategies to mitigate identified risks, conducted by institutions periodically using standardized checklists.
Steps in Performing Risk Assessment
- Gather information on hazards associated with infectious agents or materials.
- Evaluate risks related to activities that may cause exposure to these agents.
- Develop a risk control strategy that considers staff competencies and resource availability.
- Select and implement measures that align with national and institutional regulations.
- Review and continuously assess risks and control measures to ensure effectiveness.
Common Agents for Surface Decontamination
- Effective decontamination agents include heat (250° C for 15 minutes), ethylene oxide, glutaraldehyde (2%), 10% hydrogen peroxide, and 5.25% hypochlorite (10% bleach).
- Household bleach can inactivate hepatitis B virus in 10 minutes and HIV in 2 minutes.
- Bleach should remain in contact with surfaces for at least 20 minutes for effective decontamination.
Disposal of Contaminated Waste
- Strong acids or bases must be neutralized prior to disposal; do not add water to concentrated acids.
- Personal protective equipment (PPE) that may contact infectious material must be removed before exiting the laboratory and should not leave the facility.
- Contaminated PPE must be properly disposed of, ensuring it does not leave the laboratory.
Treatment of Laboratory Waste
- Uncontaminated (Non-infectious) Material: Can be reused, recycled, or disposed of as general waste.
- Contaminated Sharps (e.g., needles, scalpels): Must be collected in puncture-proof containers and treated as infectious waste.
- Contaminated Material for Reuse: Should be decontaminated before washing and treating as non-infectious.
- Contaminated Material for Disposal: Requires decontamination on-site or safe storage before transportation for further decontamination and disposal.
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