Bioreactor Setup and Yeast Inoculation Procedure

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What is the purpose of pulling back the plunger to the 20ml mark in the sampling procedure for the bioreactor?

To clear the tube of any unreacted broth (correct)

To reintroduce broth back into the bioreactor

To ensure an anaerobic environment during sampling

To collect a 20ml sample for testing

In the sampling procedure for the bioreactor, what is the purpose of injecting the sample into a 30ml capped test tube?

To ensure an aerobic reaction during testing

To clear any sediment from the sample

To start the procedures for optical density and ethanol content (correct)

To measure the glucose concentration

What must be done after taking all samples to maintain an anaerobic environment in the bioreactor?

Keep the tube clamp closed tightly (correct)

Disconnect the hose from the syringe

Inject air into the bioreactor

Ensure the tube clamp is open

What should be selected when using the CECIL 2011 Visible Spectrophotometer for determining biomass content?

A cuvette with a V on the bottom Signup and view all the answers

Why is it necessary for the blank used in determining ethanol, glucose, and yeast cell concentration to be clear?

To ensure accurate readings by not shaking it Signup and view all the answers

What is the purpose of pressing the zero button on the refractometer after placing distilled water on the lens?

To calibrate the refractometer with distilled water Signup and view all the answers

Why is it important to take 2 or 3 readings when using the refractometer for the sample distillate?

To confirm the consistency and accuracy of the measurements Signup and view all the answers

What is the reason for wiping off the lens before turning off the refractometer?

To prevent residue buildup on the lens Signup and view all the answers

Why is it advised to plot % ethanol concentration, yeast cell optical density, and glucose assay on one graph for each measurement?

To compare and analyze all data points simultaneously Signup and view all the answers

What is the primary purpose of analyzing data based on different variables studied by various groups in the experiment?

To identify experimental errors Signup and view all the answers

If the results obtained did not follow the expected path in the experiment, what should researchers do next?

Analyze potential reasons for unexpected outcomes Signup and view all the answers

What action should be taken if there are discrepancies between individual 'Original Data' and 'Results/Observations'?

Investigate and resolve any discrepancies Signup and view all the answers

Why is it important to include photocopies/scans of individual 'Original Data' in a lab report?

To provide evidence of the experiment's raw data Signup and view all the answers

'% Ethanol yield,' 'yeast biomass,' and 'glucose assay' are variables studied to understand different aspects of what in the experiment?

'Bioreactor efficiency' Signup and view all the answers

'Anaerobic environment' in the bioreactor must be maintained primarily for what reason?

'Facilitating cellular respiration' Signup and view all the answers

What is the purpose of measuring the optical density of the yeast culture in the experiment?

To indirectly measure the yeast concentration Signup and view all the answers

Why is it necessary for yeast to use extra enzymes when fermenting substrates other than glucose?

To break down the different chemical structures of non-glucose substrates Signup and view all the answers

What is the significance of the refractometer readings changing over time in the fermentation broth?

They indicate variations in sugar concentration Signup and view all the answers

Why do researchers need to compare the pH values at day 0 and day 1 of the experiment?

To observe changes in acidity during fermentation Signup and view all the answers

What change(s) in the bioreactor conditions/parameters could potentially affect the limit of % ethanol that can be obtained?

Temperature fluctuations in the bioreactor Signup and view all the answers

What is impacted by maintaining an anaerobic environment in the bioreactor for the fermentation process?

% Ethanol yield Signup and view all the answers

How does measuring yeast concentration using optical density differ from direct counting methods?

Optical density provides an indirect estimate based on cell mass and structure Signup and view all the answers

"Do the time and concentration data for glucose, ethanol, and yeast follow an expected path?" aims to assess which aspect of the experiment?

The correlation between fermentation products over time Signup and view all the answers