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Questions and Answers
What is the purpose of pulling back the plunger to the 20ml mark in the sampling procedure for the bioreactor?
What is the purpose of pulling back the plunger to the 20ml mark in the sampling procedure for the bioreactor?
- To clear the tube of any unreacted broth (correct)
- To reintroduce broth back into the bioreactor
- To ensure an anaerobic environment during sampling
- To collect a 20ml sample for testing
In the sampling procedure for the bioreactor, what is the purpose of injecting the sample into a 30ml capped test tube?
In the sampling procedure for the bioreactor, what is the purpose of injecting the sample into a 30ml capped test tube?
- To ensure an aerobic reaction during testing
- To clear any sediment from the sample
- To start the procedures for optical density and ethanol content (correct)
- To measure the glucose concentration
What must be done after taking all samples to maintain an anaerobic environment in the bioreactor?
What must be done after taking all samples to maintain an anaerobic environment in the bioreactor?
- Keep the tube clamp closed tightly (correct)
- Disconnect the hose from the syringe
- Inject air into the bioreactor
- Ensure the tube clamp is open
What should be selected when using the CECIL 2011 Visible Spectrophotometer for determining biomass content?
What should be selected when using the CECIL 2011 Visible Spectrophotometer for determining biomass content?
Why is it necessary for the blank used in determining ethanol, glucose, and yeast cell concentration to be clear?
Why is it necessary for the blank used in determining ethanol, glucose, and yeast cell concentration to be clear?
What is the purpose of pressing the zero button on the refractometer after placing distilled water on the lens?
What is the purpose of pressing the zero button on the refractometer after placing distilled water on the lens?
Why is it important to take 2 or 3 readings when using the refractometer for the sample distillate?
Why is it important to take 2 or 3 readings when using the refractometer for the sample distillate?
What is the reason for wiping off the lens before turning off the refractometer?
What is the reason for wiping off the lens before turning off the refractometer?
Why is it advised to plot % ethanol concentration, yeast cell optical density, and glucose assay on one graph for each measurement?
Why is it advised to plot % ethanol concentration, yeast cell optical density, and glucose assay on one graph for each measurement?
What is the primary purpose of analyzing data based on different variables studied by various groups in the experiment?
What is the primary purpose of analyzing data based on different variables studied by various groups in the experiment?
If the results obtained did not follow the expected path in the experiment, what should researchers do next?
If the results obtained did not follow the expected path in the experiment, what should researchers do next?
What action should be taken if there are discrepancies between individual 'Original Data' and 'Results/Observations'?
What action should be taken if there are discrepancies between individual 'Original Data' and 'Results/Observations'?
Why is it important to include photocopies/scans of individual 'Original Data' in a lab report?
Why is it important to include photocopies/scans of individual 'Original Data' in a lab report?
'% Ethanol yield,' 'yeast biomass,' and 'glucose assay' are variables studied to understand different aspects of what in the experiment?
'% Ethanol yield,' 'yeast biomass,' and 'glucose assay' are variables studied to understand different aspects of what in the experiment?
'Anaerobic environment' in the bioreactor must be maintained primarily for what reason?
'Anaerobic environment' in the bioreactor must be maintained primarily for what reason?
What is the purpose of measuring the optical density of the yeast culture in the experiment?
What is the purpose of measuring the optical density of the yeast culture in the experiment?
Why is it necessary for yeast to use extra enzymes when fermenting substrates other than glucose?
Why is it necessary for yeast to use extra enzymes when fermenting substrates other than glucose?
What is the significance of the refractometer readings changing over time in the fermentation broth?
What is the significance of the refractometer readings changing over time in the fermentation broth?
Why do researchers need to compare the pH values at day 0 and day 1 of the experiment?
Why do researchers need to compare the pH values at day 0 and day 1 of the experiment?
What change(s) in the bioreactor conditions/parameters could potentially affect the limit of % ethanol that can be obtained?
What change(s) in the bioreactor conditions/parameters could potentially affect the limit of % ethanol that can be obtained?
What is impacted by maintaining an anaerobic environment in the bioreactor for the fermentation process?
What is impacted by maintaining an anaerobic environment in the bioreactor for the fermentation process?
How does measuring yeast concentration using optical density differ from direct counting methods?
How does measuring yeast concentration using optical density differ from direct counting methods?
"Do the time and concentration data for glucose, ethanol, and yeast follow an expected path?" aims to assess which aspect of the experiment?
"Do the time and concentration data for glucose, ethanol, and yeast follow an expected path?" aims to assess which aspect of the experiment?
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