Biomolecular Detection Assays Chapter 2

True (A)

Its strong ds-DNA intercalating ability.

<p>redox</p> Signup and view all the answers

<p>Calcein (5.0mM) = Colorimetric detection MnCl2 (0.1mM) = Colorimetric detection Polyvinyl Alcohol (PVA) = Amplification unit RT-LAMP reagents = Amplification unit Sample RNA = Amplification unit</p> Signup and view all the answers

<p>Amplification unit (A)</p> Signup and view all the answers

<p>By compressing the sponge-like amplification pad using stick with a defined protrusion, which squeezes the reaction solution into the detection zone.</p> Signup and view all the answers

<p>False (B)</p> Signup and view all the answers

<p>Alcohol (A)</p> Signup and view all the answers

<p>False (B)</p> Signup and view all the answers

<p>Wound healing and oral health maintenance</p> Signup and view all the answers

<p>colorless</p> Signup and view all the answers

<p>Albumin (D)</p> Signup and view all the answers

<p>False (B)</p> Signup and view all the answers

<p>Saliva = Detection of viral diseases and drugs Urine = Monitoring kidney function and metabolic processes Cerebrospinal fluid (CSF) = Research in neurodegenerative disorders</p> Signup and view all the answers

<p>central nervous system</p> Signup and view all the answers

<p>Detection of specific proteins (B)</p> Signup and view all the answers

<p>False (B)</p> Signup and view all the answers

<p>Polymerase chain reaction (PCR)</p> Signup and view all the answers

<p>4-6 primers</p> Signup and view all the answers

<p>FIP = Forward inner primer BIP = Backward inner primer F3 = Forward outer primer B3 = Backward outer primer</p> Signup and view all the answers

<p>LAMP (B)</p> Signup and view all the answers

<p>False (B)</p> Signup and view all the answers

<p>Reverse transcription polymerase chain reaction</p> Signup and view all the answers

<p>Polymerase Chain Reaction (B)</p> Signup and view all the answers

<p>False (B)</p> Signup and view all the answers

<p>Fluorescence is recovered and increases.</p> Signup and view all the answers

<p>distance</p> Signup and view all the answers

<p>Fluorophore-labeled aptamer = Cocaine Aptamer TLS11a = Cancer cells TNF-a aptasensor = Tumor necrosis factor Methylene Blue = Redox label</p> Signup and view all the answers

<p>Incubation with target molecules (B)</p> Signup and view all the answers

<p>False (B)</p> Signup and view all the answers

<p>To decrease fluorescence.</p> Signup and view all the answers

<p>FAM (A)</p> Signup and view all the answers

<p>True (A)</p> Signup and view all the answers

<p>To detect the SARS-CoV-2 nucleoprotein.</p> Signup and view all the answers

<p>cross-linker</p> Signup and view all the answers

<p>They separate. (C)</p> Signup and view all the answers

<p>FAM = Fluorophore Eclipse = Quencher PmPD = Functional monomer for MIP Au-thin film electrode = Modification for ncovNP sensor</p> Signup and view all the answers

<p>False (B)</p> Signup and view all the answers

<p>A pre-complex.</p> Signup and view all the answers

<p>To catalyze the oxidation of TMB (D)</p> Signup and view all the answers

<p>False (B)</p> Signup and view all the answers

<p>blue</p> Signup and view all the answers

<p>biological recognition element (BRE)</p> Signup and view all the answers

<p>TMB = Colorless agent oxidized to blue Affinity-based assays = Utilize specific binding interactions Uric acid = Analyte related to chronic diseases Oxidized TMB = Resulting product of TMB oxidation</p> Signup and view all the answers

<p>652 nm (B)</p> Signup and view all the answers

<p>False (B)</p> Signup and view all the answers

<p>0.5–200 μM</p> Signup and view all the answers

Proteins and substances in saliva that indicate health conditions.

Home tests that analyze saliva for substances like drugs and alcohol.

Noninvasive evaluation of body functions, particularly kidneys.

The presence of albumin in urine, indicating potential kidney disease.

Metabolite levels linked to kidney dysfunction and prostate cancer.

Clear fluid surrounding the brain and spinal cord, indicating CNS health.

Indicators of molecular changes in the central nervous system.

Proteins promoting healing and oral health found in saliva.

A catalyst that helps oxidize TMB using hydroxyl radicals.

Highly reactive species used in the oxidation of TMB.

The blue product formed from colorless TMB through oxidation.

Biomarker related to chronic and organ-related diseases, detected in assays.

Measurement relating absorbance change to UA concentration in solutions.

Detection methods utilizing specific binding interactions for analytes.

Component that selectively binds to an analyte in affinity assays.

Device that converts binding interaction into a measurable signal.

A process where a library is incubated with target molecules, and unbound sequences are removed.

Polymerase Chain Reaction (PCR) is used to amplify target-bound sequences after selection rounds.

A method for selecting DNA aptamers through iterative rounds of selection and amplification.

Similar to DNA SELEX, it focuses on selecting RNA aptamers using reverse transcription.

An assay where a fluorescent aptamer's signal increases upon target binding, like cocaine displacing a quencher.

An aptamer-based biosensor that detects tumor necrosis factor through electrochemical signals.

A label used in aptamer assays that participates in electron transfer, changing with target binding.

A specific aptamer that binds with high affinity to cancer cells for targeted detection.

A short DNA sequence added to each end, used in fluorescence detection.

A fluorescent dye conjugated to one end of the C-strand DNA.

A molecular compound that reduces the fluorescence signal.

The configuration formed by C-strands when they hybridize.

A technique to create cavities in polymers for specific molecule recognition.

The target molecule detected using an MIP-based assay.

The modified electrode used in the ncovNP sensor development.

The functional monomer used to create the ncovNP-MIP film.

A DNA-binding redox probe used in electrochemical LAMP.

The process where a molecule, like MB, inserts itself between DNA bases.

Electric currents that reflect the oxidation/reduction state of a substance.

Double-stranded DNA, which is the common form of DNA in cells.

Loop-mediated isothermal amplification, a technique to amplify DNA.

A method that uses color change to indicate the presence of a substance.

A component that increases the concentration of DNA in a sample.

A fluorescent dye used in colorimetric detection for visible results.

A synthetic polymer designed to have specific binding sites for target molecules, like H-FABP.

Heart Fatty Acid Binding Protein, associated with chronic and organ-related diseases.

A polymer fabricated without a template, used as a control in experiments with MIPs.

The process where two complementary single-stranded nucleic acids bind to form a double-strand.

A test that measures the quantitative binding of complementary nucleic acid strands.

Reverse Transcription Polymerase Chain Reaction, a sensitive method for detecting RNA, like SARS-CoV-2.

Four essential primers used in LAMP: FIP, BIP, F3, B3 for targeting specific DNA regions.